Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,610,443	A→G	V278A (GTC→GCC)	yneH ←	putative glutaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,610,443	0	A	G	100.0%	590.3 / NA	183	V278A (GTC→GCC)	yneH	putative glutaminase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (108/75); total (108/75)

Only 100 of 187 total aligned reads displayed.

TAAACCGAACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCC  >  NC_000913/1610353‑1610532

taaACCGAACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCa                                                                                         <  1:5313758/93‑1 (MQ=255)

aaaaCCGAACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCa                                                                                         <  1:4156375/92‑1 (MQ=255)

 aaaCCGAACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAg                                                                                        >  1:2226335/1‑93 (MQ=255)

  aaCCGAACGCCCTATCTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGc                                                                                       <  2:508709/93‑1 (MQ=255)

  aaCCGAACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGc                                                                                       <  1:4753446/93‑1 (MQ=255)

     cGAACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGAt                                                                                    <  1:4809644/93‑1 (MQ=255)

     cGAACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGAt                                                                                    >  1:2964355/1‑93 (MQ=255)

       aaCGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATgg                                                                                  >  2:3488424/1‑93 (MQ=255)

       aaCGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATgg                                                                                  >  2:4311972/1‑93 (MQ=255)

        aCGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGc                                                                                 >  1:1847129/1‑93 (MQ=255)

         cGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGcc                                                                                <  1:1190953/93‑1 (MQ=255)

          gCCCTAGCTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTCCCGGACTCCAGGCAGCGATGGCCa                                                                               <  1:4106965/93‑1 (MQ=255)

          gCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCa                                                                               >  2:5808735/1‑93 (MQ=255)

          gCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCa                                                                               >  2:2255406/1‑93 (MQ=255)

            ccTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCAtt                                                                             <  2:628184/93‑1 (MQ=255)

             cTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCAttt                                                                            >  2:5026084/1‑93 (MQ=255)

             cTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCAttt                                                                            <  2:2698347/93‑1 (MQ=255)

             cTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCAttt                                                                            >  2:6033067/1‑93 (MQ=255)

              tAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTc                                                                           <  1:4907024/93‑1 (MQ=255)

              tAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTc                                                                           >  2:138330/1‑93 (MQ=255)

               aaCTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCa                                                                          >  2:5076724/1‑93 (MQ=255)

                 cTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCGTg                                                                        <  1:6175113/93‑1 (MQ=255)

                 cTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATg                                                                        <  2:3042389/93‑1 (MQ=255)

                 cTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATg                                                                        >  1:5181341/1‑93 (MQ=255)

                  tGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGc                                                                       >  1:5212778/1‑93 (MQ=255)

                  tGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGc                                                                       >  1:4609087/1‑93 (MQ=255)

                   gTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCg                                                                      >  2:5325528/1‑93 (MQ=255)

                   gTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCg                                                                      >  1:3955585/1‑93 (MQ=255)

                   gTTTCGTCAATTGTTCAAGAACGGC‑ATACCCGCCAGCCGGTTTACTGCATTATCCAGTTCCGGGCTCCCGGGAGGCATGGCCCTTTCATGCgg                                                                     >  2:5470496/1‑93 (MQ=255)

                      tCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGaa                                                                   <  2:2124902/93‑1 (MQ=255)

                       cGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAAc                                                                  <  2:1818062/93‑1 (MQ=255)

                        gTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACa                                                                 <  1:942019/93‑1 (MQ=255)

                         tCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACaa                                                                >  2:4075139/1‑93 (MQ=255)

                         tCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACaa                                                                <  2:635195/93‑1 (MQ=255)

                          cAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAAt                                                               >  1:2181777/1‑93 (MQ=255)

                          cAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAAt                                                               <  1:1674808/93‑1 (MQ=255)

                           aaTTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATc                                                              >  2:1769726/1‑93 (MQ=255)

                            aTTGTTCAAGAACGGCAATACCCGCAAGCGAGTTCCCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCg                                                             >  2:849890/1‑93 (MQ=255)

                             ttGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGc                                                            >  2:719880/1‑93 (MQ=255)

                             ttGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGc                                                            >  1:2055501/1‑93 (MQ=255)

                             ttGTGCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGc                                                            <  2:3241276/93‑1 (MQ=255)

                               gTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCca                                                          >  1:1379205/1‑93 (MQ=255)

                                 tCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCcaca                                                        >  1:367789/1‑93 (MQ=255)

                                  cAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACaa                                                       <  2:2662595/93‑1 (MQ=255)

                                  cAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACaa                                                       >  2:1828244/1‑93 (MQ=255)

                                  cAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACaa                                                       <  1:4341484/93‑1 (MQ=255)

                                    aGAACGGCAATACCCGCAAGCGAGCTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATa                                                     >  1:3711879/1‑93 (MQ=255)

                                     gAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATAc                                                    <  1:5737566/93‑1 (MQ=255)

                                      aaCGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATAcc                                                   <  1:157073/93‑1 (MQ=255)

                                      aaCGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATAcc                                                   <  1:999808/93‑1 (MQ=255)

                                          gCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGcca                                               <  1:786318/93‑1 (MQ=255)

                                            aaTACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGccacc                                             >  2:4307360/1‑93 (MQ=255)

                                            aaTACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGccacc                                             >  2:885867/1‑93 (MQ=255)

                                              tACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCaa                                           <  1:872336/93‑1 (MQ=255)

                                              tACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCaa                                           >  2:5575282/1‑93 (MQ=255)

                                              tACCCGCAAGCGAGTTA‑CTGCATCATCCAGTTCCGGACTCCAGGCTGCGATGGCCATTTCATGCTGAACAATCGCCACAATACCGCCACCAAc                                          >  2:4145894/1‑93 (MQ=255)

                                               aCCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGGCTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAAc                                          <  1:2762660/93‑1 (MQ=255)

                                               aCCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCTATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAAc                                          <  1:1425198/93‑1 (MQ=255)

                                               aCCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAAc                                          <  2:618370/93‑1 (MQ=255)

                                               aCCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAAc                                          <  1:5786419/93‑1 (MQ=255)

                                               aCCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAAc                                          >  1:1719999/1‑93 (MQ=255)

                                                cccGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACg                                         >  2:3276988/1‑93 (MQ=255)

                                                 ccGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGc                                        >  1:1079795/1‑93 (MQ=255)

                                                 ccGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGc                                        >  1:4299294/1‑93 (MQ=255)

                                                 ccGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGc                                        >  1:6074891/1‑93 (MQ=255)

                                                  cGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGcc                                       >  1:1337940/1‑93 (MQ=255)

                                                   gCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCa                                      >  1:2380308/1‑93 (MQ=255)

                                                    cAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAg                                     >  1:1053130/1‑93 (MQ=255)

                                                     aaGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAAAGCCAGa                                    >  1:1915212/1‑93 (MQ=255)

                                                      aGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGAt                                   >  2:600893/1‑93 (MQ=255)

                                                      aGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGAt                                   >  1:6111952/1‑93 (MQ=255)

                                                       gCGAGTTACCTGCATCATCCAGTTCCGGACTCCAAGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGAtt                                  >  2:5039360/1‑93 (MQ=255)

                                                         gAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTc                                <  2:515548/93‑1 (MQ=255)

                                                         gAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTc                                >  1:4787585/1‑93 (MQ=255)

                                                          aGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCCGATTTCg                               >  2:457097/1‑93 (MQ=255)

                                                          aGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCg                               >  1:4267180/1‑93 (MQ=255)

                                                             tACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCg                            <  1:1262420/93‑1 (MQ=255)

                                                               ccTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGt                          >  2:3954373/1‑93 (MQ=255)

                                                                cTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTa                         <  2:4692088/93‑1 (MQ=255)

                                                                cTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTa                         >  2:4878000/1‑93 (MQ=255)

                                                                cTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTa                         >  1:5082644/1‑93 (MQ=255)

                                                                    atcatcCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGccc                     >  2:5913219/1‑93 (MQ=255)

                                                                    atcatcCAGTTACGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGccc                     >  1:1140950/1‑93 (MQ=255)

                                                                        tcCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCTGGTAGCCCCAcc                 <  1:2528578/93‑1 (MQ=255)

                                                                        tcCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCAcc                 <  2:4025735/93‑1 (MQ=255)

                                                                         ccAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCAccc                >  2:2872958/1‑93 (MQ=255)

                                                                          cAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCg               >  1:3196001/1‑93 (MQ=255)

                                                                           aGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGc              >  1:1014667/1‑93 (MQ=255)

                                                                            gTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGcc             >  1:3153292/1‑93 (MQ=255)

                                                                             ttCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCa            <  2:4502770/93‑1 (MQ=255)

                                                                             gtCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCa            <  1:3449059/92‑1 (MQ=255)

                                                                              tCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAg           >  1:3528974/1‑93 (MQ=255)

                                                                                cGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCACCGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGc         <  2:2243019/93‑1 (MQ=255)

                                                                                 ggACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCa        >  1:4669260/1‑93 (MQ=255)

                                                                                  gACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCaa       >  1:2394161/1‑93 (MQ=255)

                                                                                   aCTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCaac      >  2:2935830/1‑92 (MQ=255)

                                                                                   aCTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCaaa      >  1:4502389/1‑93 (MQ=255)

                                                                                    cTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAAc     >  2:2403345/1‑93 (MQ=255)

                                                                                     tCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACt    >  2:1482498/1‑93 (MQ=255)

                                                                                      ccAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTc   >  1:1977715/1‑93 (MQ=255)

TAAACCGAACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCC  >  NC_000913/1610353‑1610532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AATAAACCGAACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCCCG  >  NC_000913/1610351‑1610536

AATAAACCGAACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCCGTTCCGGACTCCAGGC                                                                                              >  SRR4302533.675514/1‑94 (MQ=60)

  TAAACCGAACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAG                                                                                            >  SRR4302533.2240078/1‑94 (MQ=60)

  TAAACCGAACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAG                                                                                            <  SRR4302533.5345599/94‑1 (MQ=60)

  AAAACCGAACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAG                                                                                            <  SRR4302533.4181555/94‑1 (MQ=60)

    AACCGAACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCG                                                                                          <  SRR4302533.4782263/94‑1 (MQ=60)

      CCGAACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGAT                                                                                        >  SRR4302533.2982475/1‑94 (MQ=60)

       CGAACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATG                                                                                       >  SRR4302533.2233715/1‑94 (MQ=60)

       CGAACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATG                                                                                       >  SRR4302533.4886051/1‑94 (MQ=60)

       CGAACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATG                                                                                       <  SRR4302533.4838796/94‑1 (MQ=60)

         AACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGC                                                                                     >  SRR4302533.1858389/1‑94 (MQ=60)

           CGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCA                                                                                   <  SRR4302533.1198109/94‑1 (MQ=60)

            GCCCTAGCTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTCCCGGACTCCAGGCAGCGATGGCCAT                                                                                  <  SRR4302533.4131937/94‑1 (MQ=60)

            GCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCAT                                                                                  >  SRR4302533.1628300/1‑94 (MQ=60)

             CCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATT                                                                                 <  SRR4302533.5478641/94‑1 (MQ=60)

             CCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATT                                                                                 >  SRR4302533.124698/1‑94 (MQ=60)

              CCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTT                                                                                <  SRR4302533.1372273/94‑1 (MQ=60)

               CTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTC                                                                               >  SRR4302533.2854587/1‑94 (MQ=60)

               CTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTC                                                                               <  SRR4302533.1077757/94‑1 (MQ=60)

               CTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTC                                                                               >  SRR4302533.521086/1‑94 (MQ=60)

               CTAACGGTTTCGTCAATTGTCCAAGAACGTCAATGCCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTC                                                                               <  SRR4302533.330479/94‑1 (MQ=60)

                TAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCA                                                                              <  SRR4302533.4936754/94‑1 (MQ=60)

                TAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCA                                                                              >  SRR4302533.1478262/1‑94 (MQ=60)

                  ACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATG                                                                            >  SRR4302533.5212628/1‑94 (MQ=60)

                   CTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCGTGC                                                                           <  SRR4302533.6212218/94‑1 (MQ=60)

                   CTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGC                                                                           >  SRR4302533.7904/1‑94 (MQ=60)

                   CTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGC                                                                           >  SRR4302533.4637001/1‑94 (MQ=60)

                   CTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGC                                                                           >  SRR4302533.5244169/1‑94 (MQ=60)

                    TGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCG                                                                          >  SRR4302533.3979699/1‑94 (MQ=60)

                     GTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGG                                                                         >  SRR4302533.4976749/1‑94 (MQ=60)

                      TTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGA                                                                        >  SRR4302533.168994/1‑94 (MQ=60)

                         CGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACA                                                                     <  SRR4302533.875780/94‑1 (MQ=60)

                          GTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAA                                                                    <  SRR4302533.947561/94‑1 (MQ=60)

                           TCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAAT                                                                   >  SRR4302533.2195228/1‑94 (MQ=60)

                            CAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATC                                                                  <  SRR4302533.1684948/94‑1 (MQ=60)

                             AATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCG                                                                 >  SRR4302533.339775/1‑94 (MQ=60)

                              ATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGC                                                                >  SRR4302533.2068177/1‑94 (MQ=60)

                              ATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGC                                                                >  SRR4302533.4025512/1‑94 (MQ=60)

                              ATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGC                                                                <  SRR4302533.4266647/94‑1 (MQ=60)

                               TTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCC                                                               <  SRR4302533.5983216/94‑1 (MQ=60)

                                TGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCA                                                              >  SRR4302533.1387502/1‑94 (MQ=60)

                                  TTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACA                                                            >  SRR4302533.370014/1‑94 (MQ=60)

                                   TCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAA                                                           >  SRR4302533.2016850/1‑94 (MQ=60)

                                    CAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAAT                                                          <  SRR4302533.4367648/94‑1 (MQ=60)

                                     AAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATA                                                         <  SRR4302533.2481898/94‑1 (MQ=60)

                                     AAGAACGGCAATACCCGCAAGCGAGCTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATA                                                         >  SRR4302533.3734674/1‑94 (MQ=60)

                                       GAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACC                                                       <  SRR4302533.5772195/94‑1 (MQ=60)

                                       GAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACC                                                       <  SRR4302533.1174942/94‑1 (MQ=60)

                                        AACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCG                                                      <  SRR4302533.158073/94‑1 (MQ=60)

                                        AACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCG                                                      <  SRR4302533.1005643/94‑1 (MQ=60)

                                          CGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCC                                                    >  SRR4302533.3654252/1‑94 (MQ=60)

                                            GCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCAC                                                  <  SRR4302533.790938/94‑1 (MQ=60)

                                              AATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCA                                                >  SRR4302533.2698513/1‑94 (MQ=60)

                                               ATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAA                                               <  SRR4302533.1830112/94‑1 (MQ=60)

                                                TACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACACTACCGCCACCAAC                                              >  SRR4302533.4556653/1‑94 (MQ=60)

                                                TACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAAC                                              >  SRR4302533.1730427/1‑94 (MQ=60)

                                                TACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAAC                                              <  SRR4302533.877457/94‑1 (MQ=60)

                                                TACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAAC                                              <  SRR4302533.4834163/94‑1 (MQ=60)

                                                 ACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGGCTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACG                                             <  SRR4302533.2779595/94‑1 (MQ=60)

                                                 ACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCTATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACG                                             <  SRR4302533.1433818/94‑1 (MQ=60)

                                                 ACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACG                                             <  SRR4302533.5821371/94‑1 (MQ=60)

                                                 ACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACG                                             <  SRR4302533.3718008/94‑1 (MQ=60)

                                                 ACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACG                                             <  SRR4302533.3878064/94‑1 (MQ=60)

                                                  CCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGC                                            <  SRR4302533.2403497/94‑1 (MQ=60)

                                                  CCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGC                                            >  SRR4302533.6111636/1‑94 (MQ=60)

                                                  CCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGC                                            <  SRR4302533.6125629/94‑1 (MQ=60)

                                                  CCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGC                                            >  SRR4302533.1086158/1‑94 (MQ=60)

                                                  CCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGC                                            >  SRR4302533.4325150/1‑94 (MQ=60)

                                                   CCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCC                                           >  SRR4302533.1345991/1‑94 (MQ=60)

                                                    CGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCA                                          <  SRR4302533.1290509/94‑1 (MQ=60)

                                                    CGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCA                                          >  SRR4302533.2395031/1‑94 (MQ=60)

                                                     GCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAG                                         >  SRR4302533.3543802/1‑94 (MQ=60)

                                                     GCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAG                                         >  SRR4302533.1059333/1‑94 (MQ=60)

                                                      CAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAAAGCCAGA                                        >  SRR4302533.1926903/1‑94 (MQ=60)

                                                       AAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGAT                                       >  SRR4302533.6148843/1‑94 (MQ=60)

                                                       AAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGAT                                       <  SRR4302533.521776/94‑1 (MQ=60)

                                                          CGAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTC                                    >  SRR4302533.4816581/1‑94 (MQ=60)

                                                           GAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCG                                   <  SRR4302533.6203571/94‑1 (MQ=60)

                                                           GAGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCG                                   >  SRR4302533.4292875/1‑94 (MQ=60)

                                                            AGTTACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGC                                  >  SRR4302533.196381/1‑94 (MQ=60)

                                                               TACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGG                               >  SRR4302533.1109303/1‑94 (MQ=60)

                                                               TACCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGG                               <  SRR4302533.1269970/94‑1 (MQ=60)

                                                                 CCTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTA                             >  SRR4302533.5113364/1‑94 (MQ=60)

                                                                  CTGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAG                            <  SRR4302533.4353179/94‑1 (MQ=60)

                                                                   TGCATCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGC                           >  SRR4302533.5336624/1‑94 (MQ=60)

                                                                     CATCATCCAGTTACGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCC                         >  SRR4302533.1147746/1‑94 (MQ=60)

                                                                       TCATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCA                       <  SRR4302533.4713726/94‑1 (MQ=60)

                                                                         ATCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACC                     >  SRR4302533.4441644/1‑94 (MQ=60)

                                                                          TCCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCTGGTAGCCCCACCC                    <  SRR4302533.2544146/94‑1 (MQ=60)

                                                                           CCAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCG                   >  SRR4302533.3215666/1‑94 (MQ=60)

                                                                            CAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGC                  >  SRR4302533.1020627/1‑94 (MQ=60)

                                                                            CAGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGC                  >  SRR4302533.4684416/1‑94 (MQ=60)

                                                                             AGTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCC                 >  SRR4302533.3172676/1‑94 (MQ=60)

                                                                              GTTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCA                >  SRR4302533.191690/1‑94 (MQ=60)

                                                                               TTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAG               >  SRR4302533.3550722/1‑94 (MQ=60)

                                                                               GTCCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAG               <  SRR4302533.3470353/94‑1 (MQ=60)

                                                                                 CCGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGC             <  SRR4302533.3014112/94‑1 (MQ=60)

                                                                                  CGGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCA            >  SRR4302533.4697554/1‑94 (MQ=60)

                                                                                  CGGACCCCAGGCAGGAATGGCCATTTCATGCGGAGCAATCTCCACAATACCTCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCA            <  SRR4302533.4347786/94‑1 (MQ=60)

                                                                                   GGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAA           >  SRR4302533.2408940/1‑94 (MQ=60)

                                                                                   GGACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAA           >  SRR4302533.4309530/1‑94 (MQ=60)

                                                                                    GACTCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAA          >  SRR4302533.4529665/1‑94 (MQ=60)

                                                                                       TCCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTC       >  SRR4302533.1989826/1‑94 (MQ=60)

                                                                                        CCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCC      >  SRR4302533.2366912/1‑94 (MQ=60)

                                                                                        CCAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGAAGCCCCACCCGCCAGGCAAACTCC      <  SRR4302533.913863/94‑1 (MQ=60)

                                                                                         CAGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCC     >  SRR4302533.5626720/1‑94 (MQ=60)

                                                                                         CAGGCAGCGATGCCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCCCCGGTAGCCCCACCCGCCAGGCAAACTCCC     <  SRR4302533.6241680/94‑1 (MQ=60)

                                                                                          AGGCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCC    <  SRR4302533.3299483/94‑1 (MQ=60)

                                                                                            GCAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCCCG  >  SRR4302533.5304372/1‑94 (MQ=60)

AATAAACCGAACGCCCTAACTGTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCCCG  >  NC_000913/1610351‑1610536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 6 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`