Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,616,692 C→T G81S (GGC→AGC)  ydeB ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,616,6920CT99.5% 752.7 / ‑5.6 203G81S (GGC→AGC) ydeBhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (117/85);  total (118/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 207 total aligned reads displayed.

TCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCCGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCAAATA  >  NC_000913/1616604‑1616781
                                                                                        |                                                                                         
tctTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCt                                                                                       >  2:3909153/1‑93 (MQ=255)
 ctTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTg                                                                                      >  2:1798203/1‑93 (MQ=255)
  ttCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGctgcctgc                                                                                     <  2:2511956/93‑1 (MQ=255)
  ttCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGctgcctgc                                                                                     <  2:5845501/93‑1 (MQ=255)
  ttCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCCGCCTGc                                                                                     >  2:2812674/1‑93 (MQ=255)
   tCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCa                                                                                    >  1:2127698/1‑93 (MQ=255)
    ccGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCaa                                                                                   <  1:3784047/93‑1 (MQ=255)
     cGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAAt                                                                                  >  1:2371623/1‑93 (MQ=255)
     cGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTAGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAAt                                                                                  >  2:5136572/1‑93 (MQ=255)
      gACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAAtt                                                                                 >  2:3736768/1‑93 (MQ=255)
      gACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAAtt                                                                                 >  1:2968213/1‑93 (MQ=255)
         ttGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGc                                                                              >  2:4675160/1‑93 (MQ=255)
          tGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCa                                                                             >  2:2761047/1‑93 (MQ=255)
          tGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCa                                                                             <  1:1208270/93‑1 (MQ=255)
            cTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGa                                                                           >  1:3366785/1‑93 (MQ=255)
             ttttCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGAc                                                                          <  1:2239569/93‑1 (MQ=255)
             ttttCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGAc                                                                          <  2:1505709/93‑1 (MQ=255)
               ttCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCg                                                                        >  1:2435238/1‑93 (MQ=255)
                tCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCgg                                                                       <  1:4517620/93‑1 (MQ=255)
                 cGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCggg                                                                      <  1:3236618/93‑1 (MQ=255)
                  gCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGa                                                                     <  1:2851098/93‑1 (MQ=255)
                  gCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGa                                                                     >  1:901905/1‑93 (MQ=255)
                    cTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCCGACCGGGAAt                                                                   >  1:766516/1‑93 (MQ=255)
                    cTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAAt                                                                   >  1:5486025/1‑93 (MQ=255)
                     tCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATc                                                                  >  2:279653/1‑93 (MQ=255)
                      ccGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCg                                                                 >  2:4124119/1‑93 (MQ=255)
                      ccGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCg                                                                 >  1:3068741/1‑93 (MQ=255)
                      ccGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCg                                                                 >  1:5299078/1‑93 (MQ=255)
                       cGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGa                                                                >  2:4161602/1‑93 (MQ=255)
                       cGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGa                                                                >  1:5773210/1‑93 (MQ=255)
                         gTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGaaa                                                              <  2:2433931/93‑1 (MQ=255)
                          tGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAAt                                                             >  2:4925399/1‑93 (MQ=255)
                          tGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAAt                                                             >  1:5866468/1‑93 (MQ=255)
                           gAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATg                                                            <  2:5215532/93‑1 (MQ=255)
                              tcaatcGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCa                                                         >  2:3199442/1‑93 (MQ=255)
                               caatcGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCaa                                                        <  2:1691265/93‑1 (MQ=255)
                                aatcGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCaaa                                                       >  1:1662639/1‑93 (MQ=255)
                                  tcGCTTTCTGTTGCGGAAAGAGCATCCGAAACCCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATg                                                     <  2:2214823/93‑1 (MQ=255)
                                   cGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGt                                                    <  1:1043140/93‑1 (MQ=255)
                                   cGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGt                                                    <  2:3151486/93‑1 (MQ=255)
                                    gCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTa                                                   >  1:166915/1‑93 (MQ=255)
                                    gCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTa                                                   >  1:3895395/1‑93 (MQ=255)
                                    gCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTa                                                   >  1:4151303/1‑93 (MQ=255)
                                    gCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTa                                                   >  2:1025988/1‑93 (MQ=255)
                                    gCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTa                                                   <  1:654309/93‑1 (MQ=255)
                                      tttCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATc                                                 <  2:3721467/93‑1 (MQ=255)
                                      tttCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATc                                                 >  2:5541861/1‑93 (MQ=255)
                                        tCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCa                                               >  1:50582/1‑93 (MQ=255)
                                        tCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCa                                               <  2:439581/93‑1 (MQ=255)
                                        tCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCa                                               <  2:4540936/93‑1 (MQ=255)
                                         cTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCAt                                              <  1:2825295/93‑1 (MQ=255)
                                         cTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCAt                                              >  2:5382263/1‑93 (MQ=255)
                                          tGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATc                                             <  1:4643362/93‑1 (MQ=255)
                                           gTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCa                                            >  1:4678648/1‑93 (MQ=255)
                                           gTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCa                                            >  1:4495190/1‑93 (MQ=255)
                                               cGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAg                                        <  1:3507532/93‑1 (MQ=255)
                                                   aaGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGc                                    >  2:3779547/1‑93 (MQ=255)
                                                   aaGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGc                                    >  1:3926968/1‑93 (MQ=255)
                                                    agagCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGcc                                   >  1:569496/1‑93 (MQ=255)
                                                      agCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCg                                 <  1:1445383/93‑1 (MQ=255)
                                                       gCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCgg                                >  2:2151691/1‑93 (MQ=255)
                                                       gCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCgg                                <  1:5999549/93‑1 (MQ=255)
                                                       gCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCgg                                >  2:2882208/1‑93 (MQ=255)
                                                        cATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGc                               <  1:3169829/93‑1 (MQ=255)
                                                        cATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGc                               <  2:34259/93‑1 (MQ=255)
                                                           ccGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTa                            >  1:4858364/1‑93 (MQ=255)
                                                           ccGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTa                            <  2:4761663/93‑1 (MQ=255)
                                                              aaaaCCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATa                         <  2:4015649/93‑1 (MQ=255)
                                                              aaaaCCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATa                         >  2:5442339/1‑93 (MQ=255)
                                                                aaCCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACg                       <  2:4034489/93‑1 (MQ=255)
                                                                aaCCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACg                       >  1:2625170/1‑93 (MQ=255)
                                                                  ccGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGcc                     >  2:2715908/1‑93 (MQ=255)
                                                                  ccGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGcc                     <  1:2032227/93‑1 (MQ=255)
                                                                    gATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGccac                   >  2:3005235/1‑93 (MQ=255)
                                                                    gATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGccac                   <  1:3970310/93‑1 (MQ=255)
                                                                     aTAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGccacc                  >  2:6068241/1‑93 (MQ=255)
                                                                      tAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGccacca                 <  1:1469089/93‑1 (MQ=255)
                                                                       aaaCGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACcat                >  1:2191975/1‑93 (MQ=255)
                                                                       aaaCGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACcat                <  1:6194226/93‑1 (MQ=255)
                                                                        aaCGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACcatc               >  2:5127335/1‑93 (MQ=255)
                                                                         aCGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACcatca              <  1:6290755/93‑1 (MQ=255)
                                                                          cgcgACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACcatcat             <  1:165458/93‑1 (MQ=255)
                                                                          cgcgACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACcatcat             <  1:426486/93‑1 (MQ=255)
                                                                          cgcgACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACcatcat             <  1:4762281/93‑1 (MQ=255)
                                                                           gcgACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACcatcata            >  2:1875387/1‑92 (MQ=255)
                                                                            cgacgaTTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCatga           >  2:4884416/1‑93 (MQ=255)
                                                                            cgacgaTTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCatga           >  1:1101130/1‑93 (MQ=255)
                                                                            cgacgaTTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCatga           >  2:3587803/1‑93 (MQ=255)
                                                                             gacgaTTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCatgat          <  2:3395398/93‑1 (MQ=255)
                                                                             gacgaTTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCatgat          <  2:1203608/93‑1 (MQ=255)
                                                                             gacgaTTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCatgat          >  1:329843/1‑93 (MQ=255)
                                                                               cgaTTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATgg        <  1:1754112/93‑1 (MQ=255)
                                                                                gaTTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGc       >  2:3047812/1‑93 (MQ=255)
                                                                                gaTTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGc       >  2:630371/1‑93 (MQ=255)
                                                                                gaTTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGc       >  2:806424/1‑93 (MQ=255)
                                                                                   tAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCaaa    >  2:3307894/1‑93 (MQ=255)
                                                                                   tAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCaaa    <  2:2053306/93‑1 (MQ=255)
                                                                                   tAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCaaa    <  1:983173/93‑1 (MQ=255)
                                                                                   tAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCaaa    <  1:6292418/93‑1 (MQ=255)
                                                                                   tAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCaaa    <  1:5944550/93‑1 (MQ=255)
                                                                                        |                                                                                         
TCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCCGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCAAATA  >  NC_000913/1616604‑1616781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCCGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCAAATACGT  >  NC_000913/1616599‑1616784
                                                                                             |                                                                                            
CCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCT                                                                                              <  SRR4302533.6103257/94‑1 (MQ=60)
 CAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTG                                                                                             >  SRR4302533.763745/1‑94 (MQ=60)
 CAGCTCTTCCGACTGGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTG                                                                                             <  SRR4302533.108037/94‑1 (MQ=60)
       TTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCA                                                                                       >  SRR4302533.2140818/1‑94 (MQ=60)
        TCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAA                                                                                      <  SRR4302533.2234204/94‑1 (MQ=60)
         CCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAAT                                                                                     >  SRR4302533.2386297/1‑94 (MQ=60)
         CCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAAT                                                                                     <  SRR4302533.3807218/94‑1 (MQ=60)
          CGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATT                                                                                    >  SRR4302533.2986360/1‑94 (MQ=60)
           GACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTC                                                                                   >  SRR4302533.632794/1‑94 (MQ=60)
           GACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTC                                                                                   >  SRR4302533.3100303/1‑94 (MQ=60)
           GACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTC                                                                                   <  SRR4302533.5934011/94‑1 (MQ=60)
              TTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCA                                                                                >  SRR4302533.1702018/1‑94 (MQ=60)
               TGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAG                                                                               <  SRR4302533.1215525/94‑1 (MQ=60)
                GCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGA                                                                              >  SRR4302533.3387580/1‑94 (MQ=60)
                  TTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACC                                                                            <  SRR4302533.2253408/94‑1 (MQ=60)
                   TTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCG                                                                           <  SRR4302533.3260308/94‑1 (MQ=60)
                   TTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCG                                                                           >  SRR4302533.2450278/1‑94 (MQ=60)
                    TTCGCCGCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGG                                                                          <  SRR4302533.4809746/94‑1 (MQ=60)
                     TCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGG                                                                         <  SRR4302533.4544978/94‑1 (MQ=60)
                      CGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGA                                                                        <  SRR4302533.3256564/94‑1 (MQ=60)
                      CGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGA                                                                        >  SRR4302533.4642769/1‑94 (MQ=60)
                      CGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGA                                                                        >  SRR4302533.907194/1‑94 (MQ=60)
                       GCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAA                                                                       <  SRR4302533.2868640/94‑1 (MQ=60)
                        CCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCCGACCGGGAAT                                                                      >  SRR4302533.771040/1‑94 (MQ=60)
                        CCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAAT                                                                      <  SRR4302533.566011/94‑1 (MQ=60)
                        CCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAAT                                                                      >  SRR4302533.5519091/1‑94 (MQ=60)
                        CCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAAT                                                                      >  SRR4302533.938269/1‑94 (MQ=60)
                         CTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATC                                                                     <  SRR4302533.1666190/94‑1 (MQ=60)
                         CTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATC                                                                     >  SRR4302533.2631786/1‑94 (MQ=60)
                          TCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCG                                                                    >  SRR4302533.3087584/1‑94 (MQ=60)
                          TCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCG                                                                    >  SRR4302533.5330824/1‑94 (MQ=60)
                           CCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGA                                                                   >  SRR4302533.5808076/1‑94 (MQ=60)
                              TTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAAT                                                                >  SRR4302533.5901931/1‑94 (MQ=60)
                                GAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGC                                                              >  SRR4302533.1645158/1‑94 (MQ=60)
                                   TCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAA                                                           >  SRR4302533.4307204/1‑94 (MQ=60)
                                    CAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAA                                                          >  SRR4302533.1672689/1‑94 (MQ=60)
                                    CAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAA                                                          >  SRR4302533.2949217/1‑94 (MQ=60)
                                        CGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTA                                                      >  SRR4302533.3919164/1‑94 (MQ=60)
                                        CGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTA                                                      >  SRR4302533.4176458/1‑94 (MQ=60)
                                        CGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTA                                                      <  SRR4302533.1049274/94‑1 (MQ=60)
                                        CGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTA                                                      >  SRR4302533.1676377/1‑94 (MQ=60)
                                        CGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTA                                                      >  SRR4302533.167974/1‑94 (MQ=60)
                                         GCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTAT                                                     <  SRR4302533.658170/94‑1 (MQ=60)
                                            TTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGCTTAAGCTGCCTGCCATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCA                                                  >  SRR4302533.2501510/1‑94 (MQ=60)
                                            TTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCA                                                  >  SRR4302533.50928/1‑94 (MQ=60)
                                            TTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCA                                                  <  SRR4302533.4681902/94‑1 (MQ=60)
                                            TTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCA                                                  >  SRR4302533.333122/1‑94 (MQ=60)
                                            TTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCA                                                  >  SRR4302533.5810576/1‑94 (MQ=60)
                                             TCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCAT                                                 <  SRR4302533.2037268/94‑1 (MQ=60)
                                             TCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGAAATCGAAATGCCAAATGTATCCAT                                                 <  SRR4302533.6335523/94‑1 (MQ=60)
                                              CTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATC                                                <  SRR4302533.2842682/94‑1 (MQ=60)
                                               TGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCA                                               <  SRR4302533.4106584/94‑1 (MQ=60)
                                               TGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCA                                               >  SRR4302533.4707005/1‑94 (MQ=60)
                                               TGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCA                                               <  SRR4302533.4671495/94‑1 (MQ=60)
                                               TGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCA                                               >  SRR4302533.4522411/1‑94 (MQ=60)
                                               TGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCA                                               <  SRR4302533.3236469/94‑1 (MQ=60)
                                                 TTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACC                                             <  SRR4302533.6281000/94‑1 (MQ=60)
                                                 TTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACC                                             >  SRR4302533.4149048/1‑94 (MQ=60)
                                                    CGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGT                                          <  SRR4302533.3529162/94‑1 (MQ=60)
                                                     GGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTT                                         >  SRR4302533.244928/1‑94 (MQ=60)
                                                     GGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTT                                         >  SRR4302533.1226758/1‑94 (MQ=60)
                                                     GGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTT                                         >  SRR4302533.5121041/1‑94 (MQ=60)
                                                      GAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTG                                        >  SRR4302533.385959/1‑94 (MQ=60)
                                                       AAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGC                                       >  SRR4302533.3950941/1‑94 (MQ=60)
                                                        AAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCC                                      >  SRR4302533.572863/1‑94 (MQ=60)
                                                          GAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCG                                    <  SRR4302533.2139083/94‑1 (MQ=60)
                                                          GAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCG                                    <  SRR4302533.66240/94‑1 (MQ=60)
                                                          GAGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCG                                    >  SRR4302533.5919704/1‑94 (MQ=60)
                                                           AGCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGG                                   <  SRR4302533.1454104/94‑1 (MQ=60)
                                                            GCATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGC                                  <  SRR4302533.6035887/94‑1 (MQ=60)
                                                             CATCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCG                                 <  SRR4302533.3189338/94‑1 (MQ=60)
                                                               TCCGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTA                               >  SRR4302533.4887810/1‑94 (MQ=60)
                                                                 CGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAAT                             <  SRR4302533.2997921/94‑1 (MQ=60)
                                                                 CGAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAAT                             >  SRR4302533.803182/1‑94 (MQ=60)
                                                                  GAAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATA                            >  SRR4302533.1466022/1‑94 (MQ=60)
                                                                   AAAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATAC                           >  SRR4302533.1005791/1‑94 (MQ=60)
                                                                    AAACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACG                          >  SRR4302533.2641278/1‑94 (MQ=60)
                                                                     AACCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGC                         >  SRR4302533.5795143/1‑94 (MQ=60)
                                                                       CCGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCA                       <  SRR4302533.2044765/94‑1 (MQ=60)
                                                                        CGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCAC                      >  SRR4302533.3962225/1‑94 (MQ=60)
                                                                        CGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCAC                      >  SRR4302533.2725984/1‑94 (MQ=60)
                                                                        CGATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCAC                      >  SRR4302533.1113790/1‑94 (MQ=60)
                                                                         GATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACC                     <  SRR4302533.4536850/94‑1 (MQ=60)
                                                                         GATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACC                     <  SRR4302533.3994487/94‑1 (MQ=60)
                                                                          ATAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCA                    >  SRR4302533.1104137/1‑94 (MQ=60)
                                                                           TAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCAT                   <  SRR4302533.1477946/94‑1 (MQ=60)
                                                                           TAAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCAT                   >  SRR4302533.2205484/1‑94 (MQ=60)
                                                                            AAACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATC                  <  SRR4302533.6231393/94‑1 (MQ=60)
                                                                              ACGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCAT                <  SRR4302533.6328269/94‑1 (MQ=60)
                                                                               CGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATG               <  SRR4302533.5993021/94‑1 (MQ=60)
                                                                               CGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATG               <  SRR4302533.166509/94‑1 (MQ=60)
                                                                               CGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATG               <  SRR4302533.4791166/94‑1 (MQ=60)
                                                                               CGCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATG               <  SRR4302533.429029/94‑1 (MQ=60)
                                                                                GCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGA              >  SRR4302533.3670147/1‑94 (MQ=60)
                                                                                GCGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGA              >  SRR4302533.1107657/1‑94 (MQ=60)
                                                                                 CGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGAT             >  SRR4302533.2866313/1‑94 (MQ=60)
                                                                                 CGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGAT             <  SRR4302533.5379477/94‑1 (MQ=60)
                                                                                 CGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGAT             <  SRR4302533.2179285/94‑1 (MQ=60)
                                                                                 CGACGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGAT             >  SRR4302533.331858/1‑94 (MQ=60)
                                                                                    CGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGC          <  SRR4302533.1764796/94‑1 (MQ=60)
                                                                                    CGATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGC          <  SRR4302533.3526474/94‑1 (MQ=60)
                                                                                     GATTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCA         >  SRR4302533.476808/1‑94 (MQ=60)
                                                                                       TTAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCAAA       <  SRR4302533.6056881/94‑1 (MQ=60)
                                                                                        TAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCAAAT      <  SRR4302533.6329934/94‑1 (MQ=60)
                                                                                        TAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCAAAT      <  SRR4302533.988936/94‑1 (MQ=60)
                                                                                        TAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCAAAT      <  SRR4302533.5980500/94‑1 (MQ=60)
                                                                                        TAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCAAAT      <  SRR4302533.5077829/94‑1 (MQ=60)
                                                                                        TAAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCAAAT      >  SRR4302533.4288303/1‑94 (MQ=60)
                                                                                         AAGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCAAATA     >  SRR4302533.2306471/1‑94 (MQ=60)
                                                                                          AGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCAAATAC    <  SRR4302533.3934405/94‑1 (MQ=60)
                                                                                          AGCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCAAATAC    >  SRR4302533.3038234/1‑94 (MQ=60)
                                                                                           GCTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCACGATGGCAAATACG   >  SRR4302533.4188639/1‑94 (MQ=60)
                                                                                            CTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCAAATACGT  >  SRR4302533.6298729/1‑94 (MQ=60)
                                                                                            CTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCAAATACGT  <  SRR4302533.5247872/94‑1 (MQ=60)
                                                                                            CTGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATACATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCAAATACGT  >  SRR4302533.3405417/1‑94 (MQ=60)
                                                                                            CTGCCTGCAATTCGCAGACCGGGAATCGAAATGCAAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCAAATACGT  >  SRR4302533.4677500/1‑94 (MQ=60)
                                                                                             |                                                                                            
CCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGACGATTAAGCCGCCTGCAATTCGCAGACCGGGAATCGAAATGCCAAATGTATCCATCACCAGTTGCCCGGCGTAATACGCCACCATCATGATGGCAAATACGT  >  NC_000913/1616599‑1616784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑