Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,633,590 A→G G94G (GGT→GGC)  b1547 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,633,5900AG100.0% 666.4 / NA 210G94G (GGT→GGC) b1547hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (108/102);  total (108/102)

Only 100 of 216 total aligned reads displayed.

AAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGT  >  NC_000913/1633500‑1633677
                                                                                          |                                                                                       
    ttCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACt                                                                                   >  1:5358634/1‑93 (MQ=18)
     tCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTg                                                                                  >  1:4176847/1‑93 (MQ=25)
         gTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATa                                                                              <  2:542206/93‑1 (MQ=25)
          tATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATaa                                                                             >  1:1984859/1‑93 (MQ=255)
          tATGCAGATTTGTCAAAAGGCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATaa                                                                             >  2:6084340/1‑93 (MQ=255)
           aTGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCAGATGGCCACGGGATTGGTGCGCCGACTGGATaaa                                                                            >  2:3503752/1‑93 (MQ=255)
            tGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATaaaa                                                                           >  1:1512561/1‑93 (MQ=255)
            tGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATaaaa                                                                           >  2:2933859/1‑93 (MQ=255)
            tGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATaaaa                                                                           <  2:616544/93‑1 (MQ=255)
            tGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATaaaa                                                                           >  2:3401071/1‑93 (MQ=255)
             gCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAAc                                                                          <  1:3366805/93‑1 (MQ=255)
               aGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAActc                                                                        >  2:2133603/1‑93 (MQ=255)
               aGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAActc                                                                        <  1:3497007/93‑1 (MQ=255)
                gATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAActct                                                                       >  2:143949/1‑93 (MQ=255)
                gATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAActct                                                                       >  2:2323953/1‑93 (MQ=255)
                  tttGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGc                                                                     <  1:4395981/93‑1 (MQ=255)
                  tttGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGc                                                                     >  1:306509/1‑93 (MQ=255)
                   ttGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCa                                                                    <  2:3992319/93‑1 (MQ=255)
                    tGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCag                                                                   <  1:186704/93‑1 (MQ=255)
                     gTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCagg                                                                  <  2:7084/93‑1 (MQ=255)
                      tCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCagga                                                                 <  1:4873719/93‑1 (MQ=255)
                         aaaGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAggagga                                                              <  1:5451937/93‑1 (MQ=255)
                          aaGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGAt                                                             <  1:3249910/93‑1 (MQ=255)
                           aGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATg                                                            >  1:2148967/1‑93 (MQ=255)
                           aGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATg                                                            <  2:35587/93‑1 (MQ=255)
                           aGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATg                                                            <  2:2049744/93‑1 (MQ=255)
                            gTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGa                                                           <  1:4123478/93‑1 (MQ=255)
                            gTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATAGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGa                                                           >  2:2262677/1‑93 (MQ=255)
                              cTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGc                                                         <  1:3395955/93‑1 (MQ=255)
                               tGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGcc                                                        >  2:3976840/1‑93 (MQ=255)
                                gCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCg                                                       >  2:1698689/1‑93 (MQ=255)
                                  cccTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAg                                                     <  1:6118402/93‑1 (MQ=255)
                                  cccTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAg                                                     >  1:4044100/1‑93 (MQ=255)
                                  cccTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAg                                                     >  2:4971704/1‑93 (MQ=255)
                                   ccTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAgg                                                    <  1:4083471/93‑1 (MQ=255)
                                   ccTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAgg                                                    >  1:4294999/1‑93 (MQ=255)
                                   ccTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAgg                                                    >  2:522250/1‑93 (MQ=255)
                                     tGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGgtg                                                  <  1:1371181/93‑1 (MQ=255)
                                     tGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGgtg                                                  <  2:5945522/93‑1 (MQ=255)
                                       cATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTa                                                <  1:2852546/93‑1 (MQ=255)
                                         tCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTaaa                                              >  2:4894778/1‑93 (MQ=255)
                                         tCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTaaa                                              >  1:1914042/1‑93 (MQ=255)
                                         tCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTaaa                                              >  1:1248486/1‑93 (MQ=255)
                                           aGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGc                                            <  1:4564213/93‑1 (MQ=255)
                                            gggCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCt                                           >  1:2061582/1‑93 (MQ=255)
                                            gggCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCt                                           >  1:1149968/1‑93 (MQ=255)
                                             ggCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTg                                          >  2:2377893/1‑93 (MQ=255)
                                              gCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTgc                                         <  2:5915230/93‑1 (MQ=255)
                                               gaTAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTgcg                                        <  2:1201518/92‑1 (MQ=255)
                                               cATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTgcg                                        <  2:1555600/93‑1 (MQ=255)
                                                aTAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTgcgc                                       >  1:5694761/1‑93 (MQ=255)
                                                aTAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTgcgc                                       >  1:4947418/1‑93 (MQ=255)
                                                 tAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGcc                                      <  2:3121641/93‑1 (MQ=255)
                                                   aCCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCa                                    <  1:4246050/93‑1 (MQ=255)
                                                    ccAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCAc                                   <  1:605912/93‑1 (MQ=255)
                                                     cAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACg                                  <  1:557676/93‑1 (MQ=255)
                                                      aGACGGACCGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACgg                                 <  2:5361485/93‑1 (MQ=255)
                                                       gACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACAGc                                >  1:690032/1‑93 (MQ=255)
                                                        aCGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCg                               >  1:2699216/1‑93 (MQ=255)
                                                         cGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGa                              >  2:4293373/1‑93 (MQ=255)
                                                         cGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGa                              <  2:5528274/93‑1 (MQ=255)
                                                         cGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGa                              >  2:80202/1‑93 (MQ=255)
                                                           gAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGAcc                            <  2:5436733/93‑1 (MQ=255)
                                                           gAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGAcc                            >  1:652735/1‑93 (MQ=255)
                                                              gagTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGt                         <  1:6047315/91‑1 (MQ=255)
                                                              cGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGt                         <  1:5872097/93‑1 (MQ=255)
                                                               ggTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGtt                        >  1:5525701/1‑93 (MQ=255)
                                                                gTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGttt                       <  1:2771450/93‑1 (MQ=255)
                                                                gTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGttt                       >  2:2868851/1‑93 (MQ=255)
                                                                gTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGttt                       <  1:820846/93‑1 (MQ=255)
                                                                gTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGttt                       >  2:4671643/1‑93 (MQ=255)
                                                                  aTCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGc                     >  1:3914204/1‑93 (MQ=255)
                                                                   tCTGATGGCGACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCg                    <  2:322608/93‑1 (MQ=255)
                                                                    cTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgt                   <  2:5835534/93‑1 (MQ=255)
                                                                     tGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGGGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtc                  >  1:1767360/1‑93 (MQ=255)
                                                                      gATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcg                 <  1:2619640/93‑1 (MQ=255)
                                                                       aTGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcgg                >  2:2592442/1‑93 (MQ=255)
                                                                       aTGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcgg                >  2:440089/1‑93 (MQ=255)
                                                                        tGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcggt               >  1:136598/1‑93 (MQ=255)
                                                                        tGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcggt               >  1:5497815/1‑93 (MQ=255)
                                                                          gCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcggtcg             >  1:3541392/1‑93 (MQ=255)
                                                                          gCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcggtcg             >  1:5079188/1‑93 (MQ=255)
                                                                           ccACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGt            <  1:2234380/93‑1 (MQ=255)
                                                                            cACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTa           <  2:2088028/93‑1 (MQ=255)
                                                                             aCGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTAt          >  1:962937/1‑93 (MQ=255)
                                                                             aCGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTAt          >  1:1715429/1‑93 (MQ=255)
                                                                              cGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATc         >  1:6162761/1‑93 (MQ=255)
                                                                              cGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATc         >  1:6079597/1‑93 (MQ=255)
                                                                               gggATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATcc        >  1:4987089/1‑93 (MQ=255)
                                                                               gggATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATcc        <  2:515877/93‑1 (MQ=255)
                                                                               cggATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTTTAAAGCTGCGCCCACGGCGACCAATTTGCGTCGGTCGTATcc        <  2:6193911/92‑1 (MQ=255)
                                                                                ggATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATccc       >  1:540304/1‑93 (MQ=255)
                                                                                ggATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATccc       >  2:3745419/1‑93 (MQ=255)
                                                                                ggATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATccc       <  2:1456441/93‑1 (MQ=255)
                                                                                 gATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATTCcg      >  2:4445085/1‑93 (MQ=255)
                                                                                 gATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCcg      >  2:4493582/1‑93 (MQ=255)
                                                                                 gATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGATGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCcg      >  2:477334/1‑93 (MQ=255)
                                                                                   ttGGTGCGCCGACTGGATAAACCTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGCCCAGTTTGCGTCGGTCGTATCCcgtc    <  1:3589635/93‑1 (MQ=255)
                                                                                   ttGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCcgtc    <  2:2724250/93‑1 (MQ=255)
                                                                                     ggTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCcgtcgt  >  2:1939640/1‑93 (MQ=255)
                                                                                          |                                                                                       
AAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGT  >  NC_000913/1633500‑1633677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGA  >  NC_000913/1633497‑1633683
                                                                                             |                                                                                             
TGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCG                                                                                               >  SRR4302533.321241/1‑94 (MQ=23)
   AAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCG                                                                                            <  SRR4302533.4530583/94‑1 (MQ=12)
     GTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGAC                                                                                          >  SRR4302533.3610265/1‑94 (MQ=10)
     GTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGAC                                                                                          <  SRR4302533.6287491/94‑1 (MQ=10)
      TTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACT                                                                                         >  SRR4302533.5390801/1‑94 (MQ=10)
       TTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTG                                                                                        >  SRR4302533.4202154/1‑94 (MQ=10)
          GGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGAT                                                                                     >  SRR4302533.1157924/1‑94 (MQ=15)
            GTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAA                                                                                   >  SRR4302533.4907569/1‑94 (MQ=20)
            GTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAA                                                                                   >  SRR4302533.1997002/1‑94 (MQ=20)
              ATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAA                                                                                 >  SRR4302533.2956622/1‑94 (MQ=20)
              ATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAA                                                                                 >  SRR4302533.1521674/1‑94 (MQ=20)
               TGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAAC                                                                                <  SRR4302533.792376/94‑1 (MQ=20)
               TGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAAC                                                                                <  SRR4302533.650176/94‑1 (MQ=20)
                GCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACT                                                                               <  SRR4302533.3387600/94‑1 (MQ=20)
                 CAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTC                                                                              <  SRR4302533.5846073/94‑1 (MQ=23)
                  AGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCT                                                                             <  SRR4302533.3518571/94‑1 (MQ=25)
                  AGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCT                                                                             >  SRR4302533.4256758/1‑94 (MQ=25)
                    ATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGGACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGC                                                                           >  SRR4302533.3405635/1‑94 (MQ=29)
                    ATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGC                                                                           >  SRR4302533.308384/1‑94 (MQ=30)
                    ATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGC                                                                           >  SRR4302533.4408794/1‑94 (MQ=30)
                     TTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCA                                                                          <  SRR4302533.4422522/94‑1 (MQ=33)
                     TTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCA                                                                          >  SRR4302533.3549957/1‑94 (MQ=33)
                       TGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGG                                                                        <  SRR4302533.187864/94‑1 (MQ=38)
                         TCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAG                                                                      <  SRR4302533.4903250/94‑1 (MQ=43)
                           AAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGA                                                                    <  SRR4302533.465805/94‑1 (MQ=48)
                            AAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGAT                                                                   <  SRR4302533.5484778/94‑1 (MQ=50)
                            AAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGAT                                                                   <  SRR4302533.4031441/94‑1 (MQ=50)
                            AAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGAT                                                                   <  SRR4302533.5272251/94‑1 (MQ=50)
                            AAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGAT                                                                   <  SRR4302533.1770428/94‑1 (MQ=50)
                             AAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATG                                                                  <  SRR4302533.3269949/94‑1 (MQ=50)
                             AAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATG                                                                  >  SRR4302533.2162200/1‑94 (MQ=50)
                              AGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGA                                                                 >  SRR4302533.3241649/1‑94 (MQ=50)
                              AGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGA                                                                 <  SRR4302533.1990613/94‑1 (MQ=50)
                               GTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAG                                                                <  SRR4302533.4148513/94‑1 (MQ=50)
                               GTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAG                                                                <  SRR4302533.583993/94‑1 (MQ=50)
                               GTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAG                                                                <  SRR4302533.2602525/94‑1 (MQ=50)
                                 CTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCC                                                              <  SRR4302533.3292428/94‑1 (MQ=50)
                                 CTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCC                                                              >  SRR4302533.6110779/1‑94 (MQ=50)
                                 CTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCC                                                              <  SRR4302533.3416927/94‑1 (MQ=50)
                                    CCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAG                                                           >  SRR4302533.4068714/1‑94 (MQ=58)
                                     CCCTGCATCGGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGG                                                          <  SRR4302533.6155301/94‑1 (MQ=58)
                                     CCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGG                                                          >  SRR4302533.4320829/1‑94 (MQ=60)
                                     CCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGG                                                          <  SRR4302533.6155302/94‑1 (MQ=60)
                                     CCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGG                                                          <  SRR4302533.6196823/94‑1 (MQ=60)
                                      CCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGT                                                         >  SRR4302533.6089602/1‑94 (MQ=60)
                                      CCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGT                                                         <  SRR4302533.4108328/94‑1 (MQ=60)
                                        TGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGT                                                       <  SRR4302533.1379422/94‑1 (MQ=60)
                                          CATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAA                                                     <  SRR4302533.2870092/94‑1 (MQ=60)
                                          CATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAA                                                     >  SRR4302533.2699960/1‑94 (MQ=60)
                                           ATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAA                                                    >  SRR4302533.1255946/1‑94 (MQ=60)
                                           ATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAA                                                    >  SRR4302533.2100286/1‑94 (MQ=60)
                                           ATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAA                                                    >  SRR4302533.1925723/1‑94 (MQ=60)
                                            TCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAG                                                   <  SRR4302533.1874376/94‑1 (MQ=60)
                                             CAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGC                                                  >  SRR4302533.5473786/1‑94 (MQ=60)
                                              AGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCT                                                 >  SRR4302533.1156836/1‑94 (MQ=60)
                                              AGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCT                                                 >  SRR4302533.6079783/1‑94 (MQ=60)
                                              AGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCT                                                 <  SRR4302533.4591887/94‑1 (MQ=60)
                                              AGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCT                                                 >  SRR4302533.4173010/1‑94 (MQ=60)
                                              AGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCT                                                 >  SRR4302533.2074296/1‑94 (MQ=60)
                                               GGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTG                                                <  SRR4302533.4590521/94‑1 (MQ=60)
                                                GGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGC                                               <  SRR4302533.2734837/94‑1 (MQ=60)
                                                GGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGC                                               <  SRR4302533.4986270/94‑1 (MQ=60)
                                                  CATAACCAGACGGGACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGC                                             >  SRR4302533.2464898/1‑94 (MQ=60)
                                                  CATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGC                                             >  SRR4302533.5729153/1‑94 (MQ=60)
                                                  CATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGC                                             >  SRR4302533.5404938/1‑94 (MQ=60)
                                                  CATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGC                                             >  SRR4302533.4977371/1‑94 (MQ=60)
                                                    TAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCC                                           >  SRR4302533.329542/1‑94 (MQ=60)
                                                     AACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCA                                          >  SRR4302533.827810/1‑94 (MQ=60)
                                                      ACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCAC                                         <  SRR4302533.4271681/94‑1 (MQ=60)
                                                      ACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCAC                                         <  SRR4302533.1438945/94‑1 (MQ=60)
                                                       CCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACG                                        <  SRR4302533.609475/94‑1 (MQ=60)
                                                        CAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGG                                       <  SRR4302533.560988/94‑1 (MQ=60)
                                                         AGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGC                                      >  SRR4302533.5025581/1‑94 (MQ=60)
                                                         AGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACAGC                                      >  SRR4302533.694112/1‑94 (MQ=60)
                                                         AGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTACAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGC                                      <  SRR4302533.669603/94‑1 (MQ=60)
                                                          GACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCG                                     <  SRR4302533.4085075/94‑1 (MQ=60)
                                                          GACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCG                                     >  SRR4302533.2715773/1‑94 (MQ=60)
                                                            CGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGAC                                   <  SRR4302533.563106/94‑1 (MQ=60)
                                                             GGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACC                                  >  SRR4302533.656588/1‑94 (MQ=60)
                                                             GGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACC                                  <  SRR4302533.4298226/94‑1 (MQ=60)
                                                             GGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACC                                  <  SRR4302533.2218153/94‑1 (MQ=60)
                                                               AACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAG                                >  SRR4302533.137873/1‑94 (MQ=60)
                                                                 gaGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTT                              <  SRR4302533.6083958/92‑1 (MQ=60)
                                                                 CGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTT                              >  SRR4302533.5559059/1‑94 (MQ=60)
                                                                 CGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTT                              <  SRR4302533.5907591/94‑1 (MQ=60)
                                                                   GTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTG                            <  SRR4302533.2788454/94‑1 (MQ=60)
                                                                   GTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTG                            <  SRR4302533.5556564/94‑1 (MQ=60)
                                                                   GTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTG                            <  SRR4302533.825660/94‑1 (MQ=60)
                                                                    TATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGC                           >  SRR4302533.3938114/1‑94 (MQ=60)
                                                                    TATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGC                           >  SRR4302533.1892823/1‑94 (MQ=60)
                                                                      TCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGT                         >  SRR4302533.2814131/1‑94 (MQ=60)
                                                                       CTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTCGCGAC                        >  SRR4302533.2019247/1‑94 (MQ=60)
                                                                       CTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGGGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTC                        >  SRR4302533.1778120/1‑94 (MQ=60)
                                                                         GATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGGGCCCACGGCGACCAGTTTGCGTCGT                      >  SRR4302533.5594641/1‑94 (MQ=60)
                                                                         GATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGG                      <  SRR4302533.2635721/94‑1 (MQ=60)
                                                                          ATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGT                     >  SRR4302533.137463/1‑94 (MQ=60)
                                                                          ATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGT                     >  SRR4302533.5530974/1‑94 (MQ=60)
                                                                          ATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGT                     <  SRR4302533.170296/94‑1 (MQ=60)
                                                                            GGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCG                   >  SRR4302533.5109885/1‑94 (MQ=60)
                                                                            GGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCG                   >  SRR4302533.3563213/1‑94 (MQ=60)
                                                                              CCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTA                 >  SRR4302533.5510733/1‑94 (MQ=60)
                                                                              CCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTA                 <  SRR4302533.2248166/94‑1 (MQ=60)
                                                                               CACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTAT                >  SRR4302533.968595/1‑94 (MQ=60)
                                                                               CACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTAT                >  SRR4302533.1725830/1‑94 (MQ=60)
                                                                                ACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATC               >  SRR4302533.6116365/1‑94 (MQ=60)
                                                                                ACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATC               >  SRR4302533.6199815/1‑94 (MQ=60)
                                                                                 CGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCC              >  SRR4302533.5017326/1‑94 (MQ=60)
                                                                                  GGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCC             >  SRR4302533.543517/1‑94 (MQ=60)
                                                                                  GGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCC             >  SRR4302533.4139386/1‑94 (MQ=60)
                                                                                  GGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCC             <  SRR4302533.3974507/94‑1 (MQ=60)
                                                                                  GGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCC             >  SRR4302533.1300652/1‑94 (MQ=60)
                                                                                     ATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTC          <  SRR4302533.3479273/94‑1 (MQ=60)
                                                                                      TTGGTGCGCCGACTGGATAAACCTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGCCCAGTTTGCGTCGGTCGTATCCCGTCG         <  SRR4302533.3611735/94‑1 (MQ=60)
                                                                                      TTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCG         <  SRR4302533.3884001/94‑1 (MQ=60)
                                                                                      TTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCG         <  SRR4302533.1875747/94‑1 (MQ=60)
                                                                                        GGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTG       <  SRR4302533.539228/94‑1 (MQ=60)
                                                                                           GCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAAC    <  SRR4302533.569554/94‑1 (MQ=60)
                                                                                             GCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGA  >  SRR4302533.2779385/1‑94 (MQ=60)
                                                                                             |                                                                                             
TGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAACGA  >  NC_000913/1633497‑1633683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑