Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,640,416 (T)5→6 intergenic (+325/+97) cspF → / ← b1559 CspF/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,640,4111.T100.0% 621.7 / NA 165intergenic (+320/+102)cspF/b1559CspF/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (75/90);  total (75/90)

Only 100 of 182 total aligned reads displayed.

TAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTAC‑TTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCT  >  NC_000913/1640319‑1640498
                                                                                             |                                                                                       
 aataaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTAC‑t                                                                                        >  1:5792435/1‑93 (MQ=255)
  ataaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTAC‑tt                                                                                       <  2:1564587/93‑1 (MQ=255)
   taaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGGTTTCTAC‑ttt                                                                                      >  1:3363292/1‑93 (MQ=255)
     aGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTAC‑ttttt                                                                                    <  1:5064507/93‑1 (MQ=255)
     aGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTAC‑ttttt                                                                                    >  1:4452909/1‑93 (MQ=255)
      gTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTAC‑tttttt                                                                                   >  1:2394973/1‑92 (MQ=255)
         aaCAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTAC‑ttttttctt                                                                                >  1:3087153/1‑89 (MQ=255)
         aaCAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTAC‑ttttttctt                                                                                <  1:3225602/93‑5 (MQ=255)
         aaCAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTAC‑ttttttctt                                                                                <  2:2349572/93‑5 (MQ=255)
         aaCAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTAC‑ttttttctt                                                                                >  2:2670645/1‑89 (MQ=255)
           cAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTaa                                                                               <  2:3107484/93‑1 (MQ=255)
            aGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAAc                                                                              >  2:4814396/1‑93 (MQ=255)
            aGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAAc                                                                              >  2:2984265/1‑93 (MQ=255)
            aGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAAc                                                                              >  2:1714946/1‑93 (MQ=255)
               cATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTc                                                                           >  1:2946877/1‑93 (MQ=255)
                aTGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCg                                                                          >  2:2859460/1‑93 (MQ=255)
                aTGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCg                                                                          <  2:3033718/93‑1 (MQ=255)
                  gAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCggg                                                                        <  1:1143197/93‑1 (MQ=255)
                   aGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGt                                                                       <  2:5081435/93‑1 (MQ=255)
                    gTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGta                                                                      <  2:442980/93‑1 (MQ=255)
                       ccGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATAc                                                                   >  1:1229177/1‑93 (MQ=255)
                       ccGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATAc                                                                   <  1:1854012/93‑1 (MQ=255)
                       ccGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATAc                                                                   >  2:5607039/1‑93 (MQ=255)
                         gggATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACaa                                                                 >  1:2604730/1‑93 (MQ=255)
                           gATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAg                                                               >  2:5528513/1‑93 (MQ=255)
                           gATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAg                                                               >  1:1533908/1‑93 (MQ=255)
                            aTGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGc                                                              <  2:3703619/93‑1 (MQ=255)
                             tGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAGCCCGCTCATCAGCGGGTTTTCTACGTTTTTCTTAACGTCGGGTATACAAAGcc                                                             <  2:2060371/93‑1 (MQ=255)
                              gagaTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGccc                                                            <  1:2768665/93‑1 (MQ=255)
                               agaTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCa                                                           <  2:4368100/93‑1 (MQ=255)
                               agaTTCAACATTACCATTGCCCCA‑TTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCAt                                                          <  1:176591/93‑1 (MQ=255)
                                gaTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCAt                                                          >  1:3780418/1‑93 (MQ=255)
                                gaTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCAt                                                          <  1:1351022/93‑1 (MQ=255)
                                 aTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATc                                                         <  1:3243095/93‑1 (MQ=255)
                                 aTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATc                                                         <  2:38117/93‑1 (MQ=255)
                                 aTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATc                                                         <  2:1105734/93‑1 (MQ=255)
                                   tCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGt                                                       <  2:806705/93‑1 (MQ=255)
                                    cAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGtt                                                      >  2:5193543/1‑93 (MQ=255)
                                      aCATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGa                                                    <  1:45034/93‑1 (MQ=255)
                                      aCATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGa                                                    >  2:4083854/1‑93 (MQ=255)
                                      aCATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGa                                                    <  2:2566996/93‑1 (MQ=255)
                                        aTTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaaa                                                  >  1:3686297/1‑93 (MQ=255)
                                         ttACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaaaa                                                 >  2:573130/1‑93 (MQ=255)
                                           aCCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGTGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaaaaaa                                               <  2:3438292/93‑1 (MQ=255)
                                           aCCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaaaaaa                                               >  1:5875600/1‑93 (MQ=255)
                                             cATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAAtt                                             >  2:4526400/1‑93 (MQ=255)
                                              aTTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAAttt                                            <  1:3043190/93‑1 (MQ=255)
                                                 gCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTAt                                         <  1:1966318/93‑1 (MQ=255)
                                                   cccATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGGATACAAAGCCCATCGTTGAAAAAATTTTATcc                                       >  1:5441879/1‑93 (MQ=255)
                                                    ccATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCa                                      <  2:3012426/93‑1 (MQ=255)
                                                    ccATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCa                                      <  2:5726069/93‑1 (MQ=255)
                                                     cATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCat                                     >  2:4855571/1‑93 (MQ=255)
                                                      aTTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCata                                    >  2:2177664/1‑93 (MQ=255)
                                                      aTTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCata                                    <  1:2184254/93‑1 (MQ=255)
                                                      aTTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCata                                    <  2:1700399/93‑1 (MQ=255)
                                                      aTTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCata                                    >  2:5368353/1‑93 (MQ=255)
                                                      aTTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCata                                    <  1:5523665/93‑1 (MQ=255)
                                                       tttAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCatat                                   >  1:5607864/1‑93 (MQ=255)
                                                       tttAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCatat                                   <  1:2654662/93‑1 (MQ=255)
                                                        ttAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAtt                                  >  2:4699077/1‑93 (MQ=255)
                                                           aaGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAttttt                               <  1:2524875/93‑1 (MQ=255)
                                                            aGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAtttttt                              >  1:1669208/1‑93 (MQ=255)
                                                            aGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAtttttt                              >  2:5262785/1‑93 (MQ=255)
                                                            aGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAtttttt                              <  2:4593576/93‑1 (MQ=255)
                                                             gCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATTCATAttttttt                             >  2:1151732/1‑92 (MQ=255)
                                                                 aaaaCCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGaaaa                         >  1:6158287/1‑93 (MQ=255)
                                                                 aaaaCCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGaaaa                         >  1:3569132/1‑93 (MQ=255)
                                                                  aaaCCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGaaaaa                        <  1:588359/93‑1 (MQ=255)
                                                                  aaaCCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGaaaaa                        >  2:3299977/1‑93 (MQ=255)
                                                                     cccGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGc                     <  1:3287922/93‑1 (MQ=255)
                                                                     cccGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGc                     >  1:2416039/1‑93 (MQ=255)
                                                                      ccGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGGAAAATGCa                    >  2:1501122/1‑93 (MQ=255)
                                                                      ccGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCa                    <  1:4664572/93‑1 (MQ=255)
                                                                      ccGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGGATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCa                    >  2:1943721/1‑93 (MQ=255)
                                                                        gCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCaaa                  >  1:5656607/1‑93 (MQ=255)
                                                                        gCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCaaa                  <  2:1381573/93‑1 (MQ=255)
                                                                         cTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAc                 >  1:434357/1‑93 (MQ=255)
                                                                         cTCATCAGCGGGCTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTTAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAc                 >  2:4955273/1‑93 (MQ=255)
                                                                           catcaGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcat               <  2:1232299/93‑1 (MQ=255)
                                                                           catcaGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcat               >  1:2101693/1‑93 (MQ=255)
                                                                            atcaGCGTGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcatc              <  1:5128520/93‑1 (MQ=255)
                                                                            atcaGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcatc              <  1:1501231/93‑1 (MQ=255)
                                                                            atcaGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcatc              <  2:3750277/93‑1 (MQ=255)
                                                                             tcaGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcatca             >  2:3146204/1‑93 (MQ=255)
                                                                             tcaGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcatca             >  2:2328748/1‑93 (MQ=255)
                                                                              caGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcatcat            <  1:3701982/93‑1 (MQ=255)
                                                                              caGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcatcat            <  1:41672/93‑1 (MQ=255)
                                                                              caGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcatcat            <  1:918609/93‑1 (MQ=255)
                                                                                gCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGt          <  2:4344787/93‑1 (MQ=255)
                                                                                gCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGt          <  2:4171801/93‑1 (MQ=255)
                                                                                gCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGt          >  1:811436/1‑93 (MQ=255)
                                                                                gCGGGTTTGCTACTTTTTTCATAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGt          >  2:6163916/1‑93 (MQ=255)
                                                                                gCGGGGTTTCTACTTTTTTCTTAACGTCGGGGATACAAAGCCCCTCGTTGAAAAAATTTTATCCCTATTTTTTGAAAAATGCAAACATCATGt          >  2:557512/1‑93 (MQ=255)
                                                                                 cGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTc         <  1:1374913/93‑1 (MQ=255)
                                                                                 cGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTc         <  1:4055460/93‑1 (MQ=255)
                                                                                    gTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGcc      >  2:4252605/1‑93 (MQ=255)
                                                                                     ttttCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCa     >  1:5432143/1‑93 (MQ=255)
                                                                                       ttCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATc   <  2:357876/93‑1 (MQ=255)
                                                                                       ttCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATc   <  2:18152/93‑1 (MQ=255)
                                                                                        tCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCt  >  1:1547391/1‑93 (MQ=255)
                                                                                             |                                                                                       
TAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTAC‑TTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCT  >  NC_000913/1640319‑1640498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTAC‑TTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAA  >  NC_000913/1640323‑1640508
                                                                                              |                                                                                            
AAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTAC‑TTTTT                                                                                              >  SRR4302533.4479831/1‑94 (MQ=60)
 AGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTAC‑TTTTTT                                                                                             >  SRR4302533.2409757/1‑94 (MQ=60)
 AGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTAC‑TTTTTT                                                                                             <  SRR4302533.5095101/94‑1 (MQ=60)
   TTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTAC‑TTTTTtct                                                                                           >  SRR4302533.5576249/1‑91 (MQ=60)
    TAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTT                                                                                           >  SRR4302533.3106134/1‑94 (MQ=60)
     AACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTA                                                                                          <  SRR4302533.3245489/94‑1 (MQ=60)
      ACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAA                                                                                         <  SRR4302533.5434748/94‑1 (MQ=60)
      ACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAA                                                                                         <  SRR4302533.4356365/94‑1 (MQ=60)
       CAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAAC                                                                                        >  SRR4302533.4130834/1‑94 (MQ=60)
          ACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTC                                                                                     >  SRR4302533.2964898/1‑94 (MQ=60)
           CATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCG                                                                                    <  SRR4302533.1268014/94‑1 (MQ=60)
           CATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCG                                                                                    <  SRR4302533.3094390/94‑1 (MQ=60)
           CATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCG                                                                                    <  SRR4302533.4283430/94‑1 (MQ=60)
           CATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCG                                                                                    <  SRR4302533.3335634/94‑1 (MQ=60)
            ATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGG                                                                                   <  SRR4302533.2216315/94‑1 (MQ=60)
            ATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGG                                                                                   >  SRR4302533.1875810/1‑94 (MQ=60)
             TGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGG                                                                                  <  SRR4302533.614752/94‑1 (MQ=60)
             TGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGG                                                                                  >  SRR4302533.989389/1‑94 (MQ=60)
              GAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGT                                                                                 <  SRR4302533.1150014/94‑1 (MQ=60)
                GTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTAT                                                                               <  SRR4302533.136575/94‑1 (MQ=60)
                 TCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATA                                                                              >  SRR4302533.2797317/1‑94 (MQ=60)
                  CCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATAC                                                                             >  SRR4302533.1236536/1‑94 (MQ=60)
                   CCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACA                                                                            <  SRR4302533.1865315/94‑1 (MQ=60)
                    CGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAA                                                                           <  SRR4302533.175433/94‑1 (MQ=60)
                    CGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAA                                                                           >  SRR4302533.2620731/1‑94 (MQ=60)
                      GGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCTGGTATACAAAG                                                                         >  SRR4302533.3115350/1‑94 (MQ=60)
                      GGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAG                                                                         >  SRR4302533.1543121/1‑94 (MQ=60)
                         TGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCC                                                                      <  SRR4302533.374430/94‑1 (MQ=60)
                          GAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCA                                                                     <  SRR4302533.2785654/94‑1 (MQ=60)
                           AGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATATAAAGCCCAT                                                                    <  SRR4302533.3015016/94‑1 (MQ=60)
                           AGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCAT                                                                    >  SRR4302533.3803578/1‑94 (MQ=60)
                           AGATTCAACATTACCATTGCCCCA‑TTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATC                                                                   <  SRR4302533.177701/94‑1 (MQ=60)
                            GATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATC                                                                   <  SRR4302533.3668561/94‑1 (MQ=60)
                            GATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATC                                                                   <  SRR4302533.1359147/94‑1 (MQ=60)
                             ATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCG                                                                  <  SRR4302533.3263082/94‑1 (MQ=60)
                                CAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTG                                                               <  SRR4302533.3303183/94‑1 (MQ=60)
                                  ACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAA                                                             <  SRR4302533.45346/94‑1 (MQ=60)
                                   CATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAA                                                            >  SRR4302533.3708954/1‑94 (MQ=60)
                                    ATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAA                                                           >  SRR4302533.4715460/1‑94 (MQ=60)
                                     TTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAA                                                          <  SRR4302533.2552104/94‑1 (MQ=60)
                                      TACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAA                                                         >  SRR4302533.5911119/1‑94 (MQ=60)
                                          ATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTT                                                     >  SRR4302533.1417460/1‑94 (MQ=60)
                                          ATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTT                                                     <  SRR4302533.3061819/94‑1 (MQ=60)
                                            TGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTAT                                                   >  SRR4302533.5675079/1‑94 (MQ=60)
                                             GCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATC                                                  >  SRR4302533.159970/1‑94 (MQ=60)
                                             GCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATC                                                  <  SRR4302533.1978333/94‑1 (MQ=60)
                                              CCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGGATACAAAGCCCATCGTTGAAAAAATTTTATCC                                                 >  SRR4302533.5474658/1‑94 (MQ=60)
                                                  ATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAT                                             <  SRR4302533.2197718/94‑1 (MQ=60)
                                                  ATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAT                                             >  SRR4302533.5641749/1‑94 (MQ=60)
                                                  ATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAT                                             <  SRR4302533.5557015/94‑1 (MQ=60)
                                                  ATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAT                                             >  SRR4302533.2729283/1‑94 (MQ=60)
                                                   TTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATT                                            <  SRR4302533.2670968/94‑1 (MQ=60)
                                                       AAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTT                                        >  SRR4302533.1679292/1‑94 (MQ=60)
                                                       AAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTT                                        <  SRR4302533.2540423/94‑1 (MQ=60)
                                                           ACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAA                                    <  SRR4302533.5136751/94‑1 (MQ=60)
                                                            CAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAA                                   >  SRR4302533.3591130/1‑94 (MQ=60)
                                                            CAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAA                                   >  SRR4302533.6195328/1‑94 (MQ=60)
                                                              AAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAAT                                 <  SRR4302533.591832/94‑1 (MQ=60)
                                                              AAACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAAT                                 >  SRR4302533.2055091/1‑94 (MQ=60)
                                                                ACCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGC                               >  SRR4302533.2430962/1‑94 (MQ=60)
                                                                 CCCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCA                              <  SRR4302533.3308216/94‑1 (MQ=60)
                                                                  CCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAA                             <  SRR4302533.4692833/94‑1 (MQ=60)
                                                                  CCGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAA                             >  SRR4302533.4026789/1‑94 (MQ=60)
                                                                   CGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAA                            <  SRR4302533.1513612/94‑1 (MQ=60)
                                                                   CGCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAA                            >  SRR4302533.5690790/1‑94 (MQ=60)
                                                                    GCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAC                           <  SRR4302533.5518025/94‑1 (MQ=60)
                                                                    GCTCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAC                           >  SRR4302533.436946/1‑94 (MQ=60)
                                                                      TCATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACAT                         >  SRR4302533.2114653/1‑94 (MQ=60)
                                                                        ATCAGCGTGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCA                       <  SRR4302533.5159502/94‑1 (MQ=60)
                                                                        ATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCA                       <  SRR4302533.1510279/94‑1 (MQ=60)
                                                                        ATCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCA                       <  SRR4302533.820034/94‑1 (MQ=60)
                                                                         TCAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCAT                      <  SRR4302533.720375/94‑1 (MQ=60)
                                                                          CAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATG                     <  SRR4302533.3724713/94‑1 (MQ=60)
                                                                          CAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATG                     <  SRR4302533.924007/94‑1 (MQ=60)
                                                                          CAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATG                     <  SRR4302533.41955/94‑1 (MQ=60)
                                                                          CAGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATG                     <  SRR4302533.1813080/94‑1 (MQ=60)
                                                                           AGCGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGT                    >  SRR4302533.816191/1‑94 (MQ=60)
                                                                             CGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCG                  >  SRR4302533.3439198/1‑94 (MQ=60)
                                                                             CGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCG                  <  SRR4302533.1383186/94‑1 (MQ=60)
                                                                             CGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCG                  <  SRR4302533.4080151/94‑1 (MQ=60)
                                                                             CGGGTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCG                  <  SRR4302533.4644440/94‑1 (MQ=60)
                                                                                GTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCA               >  SRR4302533.5464855/1‑94 (MQ=60)
                                                                                GTTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCA               >  SRR4302533.589062/1‑94 (MQ=60)
                                                                                GTTTTCTACTTTTTTCTTAACGTCGCGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGACGCCA               >  SRR4302533.5412111/1‑94 (MQ=60)
                                                                                 TTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCAT              >  SRR4302533.2454666/1‑94 (MQ=60)
                                                                                 TTTTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCAT              <  SRR4302533.5771642/94‑1 (MQ=60)
                                                                                   TTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCT            <  SRR4302533.4437084/94‑1 (MQ=60)
                                                                                   TTCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCT            >  SRR4302533.1556715/1‑94 (MQ=60)
                                                                                    TCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTT           >  SRR4302533.2488900/1‑94 (MQ=60)
                                                                                    TCTACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGGCGCCATCTT           >  SRR4302533.3891044/1‑94 (MQ=60)
                                                                                      TACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCA         <  SRR4302533.339516/94‑1 (MQ=60)
                                                                                      TACTTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCA         >  SRR4302533.2652460/1‑94 (MQ=60)
                                                                                        ctTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGC       >  SRR4302533.5685332/3‑94 (MQ=60)
                                                                                        ctTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGC       >  SRR4302533.1411188/3‑94 (MQ=60)
                                                                                        ctTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGC       <  SRR4302533.1822378/92‑1 (MQ=60)
                                                                                        T‑TTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCA      <  SRR4302533.3815370/94‑1 (MQ=60)
                                                                                        T‑TTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCA      <  SRR4302533.3747007/94‑1 (MQ=60)
                                                                                             TTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAA  >  SRR4302533.3809290/1‑94 (MQ=60)
                                                                                             TTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAA  >  SRR4302533.6029828/1‑94 (MQ=60)
                                                                                              |                                                                                            
AAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTAC‑TTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAA  >  NC_000913/1640323‑1640508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑