Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,652,626 T→C E180E (GAA→GAG)  rspA ← starvation sensing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,652,6260TC99.5% 672.0 / ‑4.5 208E180E (GAA→GAG) rspAstarvation sensing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (106/101);  minor base A (0/1);  total (106/102)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 225 total aligned reads displayed.

CAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCC  >  NC_000913/1652536‑1652715
                                                                                          |                                                                                         
cagcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctc                                                                                         <  2:6255899/93‑1 (MQ=255)
 agcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctct                                                                                        <  1:6217219/93‑1 (MQ=255)
 agcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctct                                                                                        <  1:3332423/93‑1 (MQ=255)
    aaaTGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTcc                                                                                     <  1:108707/93‑1 (MQ=255)
    aaaTGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTcc                                                                                     >  2:1157479/1‑93 (MQ=255)
     aaTGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCg                                                                                    <  2:1440537/93‑1 (MQ=255)
      aTGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCgg                                                                                   <  1:4957687/93‑1 (MQ=255)
       tGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGc                                                                                  >  1:6287190/1‑93 (MQ=255)
       tGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGc                                                                                  >  1:779525/1‑93 (MQ=255)
          tCATTAAAACCAATCTTGTTACGTACCGCGTCAAAGAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCAc                                                                               <  1:2768175/93‑1 (MQ=255)
          tCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCAc                                                                               >  2:1081227/1‑93 (MQ=255)
          tCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCAc                                                                               >  2:3111278/1‑93 (MQ=255)
          tCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCAc                                                                               <  1:3118324/93‑1 (MQ=255)
          tCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCAc                                                                               >  2:2239542/1‑93 (MQ=255)
           cATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACt                                                                              <  1:1014641/93‑1 (MQ=255)
           cATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACt                                                                              >  1:4508235/1‑93 (MQ=255)
            aTTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTg                                                                             <  2:203009/93‑1 (MQ=255)
            aTTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTg                                                                             >  1:3550906/1‑93 (MQ=255)
            aTTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGGATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTg                                                                             >  2:408014/1‑93 (MQ=255)
            aTTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGGATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTg                                                                             >  1:1960283/1‑93 (MQ=255)
             ttAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGt                                                                            >  2:4920070/1‑93 (MQ=255)
               aaaaCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTcc                                                                          <  1:1505073/93‑1 (MQ=255)
               aaaaCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTcc                                                                          <  1:5947741/93‑1 (MQ=255)
               aaaaCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTcc                                                                          >  2:2956338/1‑93 (MQ=255)
               aaaaCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTcc                                                                          <  2:2913119/93‑1 (MQ=255)
                 aaCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCtt                                                                        <  1:680933/93‑1 (MQ=255)
                 aaCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCtt                                                                        >  2:4177852/1‑93 (MQ=255)
                  aCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCttt                                                                       >  2:1590716/1‑93 (MQ=255)
                   ccAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTg                                                                      >  1:1691974/1‑93 (MQ=255)
                     aaaCTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGt                                                                    <  1:209862/93‑1 (MQ=255)
                     aaaCTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGt                                                                    >  1:1308600/1‑93 (MQ=255)
                      aaCTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGtt                                                                   <  2:4860129/93‑1 (MQ=255)
                        cTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGc                                                                 >  2:3517158/1‑93 (MQ=255)
                         ttGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCg                                                                >  2:5032380/1‑93 (MQ=255)
                          tGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCgg                                                               <  2:248098/93‑1 (MQ=255)
                          tGTTACGTACCGCGTCAAACAAGTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCgg                                                               <  1:1485212/93‑1 (MQ=255)
                           gTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCggg                                                              >  1:3785298/1‑93 (MQ=255)
                            ttACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGt                                                             >  1:2658817/1‑93 (MQ=255)
                            ttACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGt                                                             <  2:6108957/93‑1 (MQ=255)
                            ttACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGt                                                             <  2:4224827/93‑1 (MQ=255)
                             tACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGtt                                                            <  2:5952273/93‑1 (MQ=255)
                              aCGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTc                                                           >  1:4032749/1‑93 (MQ=255)
                                gTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCAt                                                         <  2:1664716/93‑1 (MQ=255)
                                gTACCGCGTCAAACAATTTCGGCATGAAAGCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCAt                                                         <  1:1797134/93‑1 (MQ=255)
                                  aCCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                                       >  2:554361/1‑93 (MQ=255)
                                    cgcgTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGc                                                     <  2:3418646/93‑1 (MQ=255)
                                     gcgTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGcc                                                    >  1:5154968/1‑93 (MQ=255)
                                      cgTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCa                                                   <  1:2601741/93‑1 (MQ=255)
                                        tCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGa                                                 >  1:5097390/1‑93 (MQ=255)
                                         cAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGac                                                <  2:2582177/93‑1 (MQ=255)
                                          aaaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacc                                               >  1:5193578/1‑93 (MQ=255)
                                          aaaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacc                                               >  2:3240063/1‑93 (MQ=255)
                                          aaaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacc                                               >  1:1581754/1‑93 (MQ=255)
                                            aCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctt                                             >  2:3382306/1‑93 (MQ=255)
                                             cAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGaccttt                                            >  2:2669065/1‑93 (MQ=255)
                                             cAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGaccttt                                            >  1:894667/1‑93 (MQ=255)
                                             cAATTTCGGCATGAAATCGAGGTATTTCTCCATCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGaccttt                                            <  2:5505091/93‑1 (MQ=255)
                                                tttCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacc                                         <  2:1500013/93‑1 (MQ=255)
                                                  tCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacctt                                       <  2:884232/93‑1 (MQ=255)
                                                   cGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttaccttt                                      <  1:6007510/93‑1 (MQ=255)
                                                    ggCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTc                                     <  1:4144804/93‑1 (MQ=255)
                                                    ggCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTc                                     <  1:835961/93‑1 (MQ=255)
                                                    ggCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTc                                     >  1:5387872/1‑93 (MQ=255)
                                                        tGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACa                                 >  1:4263418/1‑93 (MQ=255)
                                                         gAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTCTACCTTTCGACAt                                <  1:81895/93‑1 (MQ=255)
                                                          aaaTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATg                               <  1:5597277/93‑1 (MQ=255)
                                                           aaTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGc                              >  2:4632159/1‑93 (MQ=255)
                                                             tCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCg                            >  2:1406524/1‑93 (MQ=255)
                                                              cGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGt                           <  1:258390/93‑1 (MQ=255)
                                                              cGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGt                           <  2:5097633/93‑1 (MQ=255)
                                                               gAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTa                          >  1:3700005/1‑93 (MQ=255)
                                                               gAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTa                          <  1:4804242/93‑1 (MQ=255)
                                                               gAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGCTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGtg                          >  2:2697647/1‑92 (MQ=255)
                                                               gAGGTATTTCTCCGTCGACCACAGCTGCTCGTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTa                          <  1:1302818/93‑1 (MQ=255)
                                                                aGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAg                         <  2:516033/93‑1 (MQ=255)
                                                                aGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAg                         >  2:5076423/1‑93 (MQ=255)
                                                                aGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAg                         <  1:6205800/93‑1 (MQ=255)
                                                                aGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAg                         >  2:4490080/1‑93 (MQ=255)
                                                                 ggTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAgg                        <  1:5716248/93‑1 (MQ=255)
                                                                  gTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggt                       >  2:4548379/1‑93 (MQ=255)
                                                                  gTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggt                       <  2:2390254/93‑1 (MQ=255)
                                                                   tATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtg                      <  1:5067571/93‑1 (MQ=255)
                                                                   tATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtg                      <  2:2327700/93‑1 (MQ=255)
                                                                   tATTTCTCCGTCGACCACAGCTGATCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtg                      <  2:4311421/93‑1 (MQ=255)
                                                                    aTTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtgg                     >  2:4737287/1‑93 (MQ=255)
                                                                     ttgctcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtggt                    <  2:3577580/90‑1 (MQ=255)
                                                                       tctcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGttt                  >  2:1787835/1‑93 (MQ=255)
                                                                        ctcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGtttt                 <  1:964533/93‑1 (MQ=255)
                                                                        ctcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGtttt                 >  1:2454280/1‑93 (MQ=255)
                                                                          ccGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCa               >  1:5783979/1‑93 (MQ=255)
                                                                           cGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCAt              >  2:5473229/1‑93 (MQ=255)
                                                                            gTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATa             <  1:2876371/93‑1 (MQ=255)
                                                                             tCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAc            >  2:3829228/1‑93 (MQ=255)
                                                                                gccACTGCTGCTCTGCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGTCCTTTCCCTTTCGACATGCCGTAGGTGGTTTTCATACCAg         <  2:1911141/92‑1 (MQ=255)
                                                                                aCCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAg         >  1:2851106/1‑93 (MQ=255)
                                                                                 ccACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGTGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATGCCAgg        <  2:1795037/93‑1 (MQ=255)
                                                                                      gctgctCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTc   >  2:1125329/1‑93 (MQ=255)
                                                                                      gctgctCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTc   >  2:435183/1‑93 (MQ=255)
                                                                                      gctgctCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTc   <  2:5686056/93‑1 (MQ=255)
                                                                                       cTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTcc  >  1:1783516/1‑93 (MQ=255)
                                                                                          |                                                                                         
CAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCC  >  NC_000913/1652536‑1652715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGCAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCAC  >  NC_000913/1652533‑1652719
                                                                                             |                                                                                             
ATGCAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGC                                                                                               >  SRR4302533.4549394/1‑94 (MQ=60)
 TGCAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCT                                                                                              <  SRR4302533.1108772/94‑1 (MQ=60)
   CAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCT                                                                                            >  SRR4302533.3628447/1‑94 (MQ=60)
    AGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTT                                                                                           <  SRR4302533.6254498/94‑1 (MQ=60)
    AGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTT                                                                                           <  SRR4302533.1801801/94‑1 (MQ=60)
    AGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTT                                                                                           <  SRR4302533.3353016/94‑1 (MQ=60)
      CAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCC                                                                                         <  SRR4302533.4506007/94‑1 (MQ=60)
       AAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCG                                                                                        <  SRR4302533.109393/94‑1 (MQ=60)
         ATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGC                                                                                      >  SRR4302533.6324699/1‑94 (MQ=60)
         ATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGC                                                                                      >  SRR4302533.784117/1‑94 (MQ=60)
         ATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGC                                                                                      <  SRR4302533.4987722/94‑1 (MQ=60)
         ATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGC                                                                                      <  SRR4302533.107417/94‑1 (MQ=60)
          TGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCC                                                                                     >  SRR4302533.2800482/1‑94 (MQ=60)
           GTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCA                                                                                    <  SRR4302533.3178195/94‑1 (MQ=60)
             TCATTAAAACCAATCTTGTTACGTACCGCGTCAAAGAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACT                                                                                  <  SRR4302533.2785156/94‑1 (MQ=60)
             TCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACT                                                                                  <  SRR4302533.3137498/94‑1 (MQ=60)
             TCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACT                                                                                  >  SRR4302533.4535538/1‑94 (MQ=60)
              CATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTG                                                                                 >  SRR4302533.3572780/1‑94 (MQ=60)
              CATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTG                                                                                 <  SRR4302533.603622/94‑1 (MQ=60)
              CATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTG                                                                                 <  SRR4302533.1020595/94‑1 (MQ=60)
              CATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGGATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTG                                                                                 >  SRR4302533.1972246/1‑94 (MQ=60)
               ATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGT                                                                                >  SRR4302533.2010392/1‑94 (MQ=60)
                 TAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCC                                                                              <  SRR4302533.6203207/94‑1 (MQ=60)
                  AAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCT                                                                             <  SRR4302533.5983716/94‑1 (MQ=60)
                  AAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCT                                                                             <  SRR4302533.1514152/94‑1 (MQ=60)
                   AAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTT                                                                            <  SRR4302533.5149803/94‑1 (MQ=60)
                   AAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTT                                                                            <  SRR4302533.851633/94‑1 (MQ=60)
                    AACCAAACTTGTTACGTGCCGCGTCAAACCATTTCGGAATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTT                                                                           <  SRR4302533.3362232/94‑1 (MQ=60)
                    AACCAAACTTGTTACGTACCGCGTCAGACAATTTCGTCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTT                                                                           >  SRR4302533.5719861/1‑94 (MQ=60)
                    AACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTT                                                                           <  SRR4302533.1741609/94‑1 (MQ=60)
                    AACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTT                                                                           <  SRR4302533.684963/94‑1 (MQ=60)
                     ACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTG                                                                          <  SRR4302533.5033994/94‑1 (MQ=60)
                     ACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTG                                                                          >  SRR4302533.1702222/1‑94 (MQ=60)
                       CAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGT                                                                        >  SRR4302533.6316439/1‑94 (MQ=60)
                       CAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGT                                                                        >  SRR4302533.1316456/1‑94 (MQ=60)
                        AAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTT                                                                       <  SRR4302533.2163980/94‑1 (MQ=60)
                        AAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTT                                                                       <  SRR4302533.211187/94‑1 (MQ=60)
                         AACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTG                                                                      >  SRR4302533.833308/1‑94 (MQ=60)
                           CTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCG                                                                    >  SRR4302533.4240307/1‑94 (MQ=60)
                            TTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGG                                                                   >  SRR4302533.3505312/1‑94 (MQ=60)
                            TTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGG                                                                   >  SRR4302533.1520220/1‑94 (MQ=60)
                             TGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGG                                                                  <  SRR4302533.3341891/94‑1 (MQ=60)
                             TGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGG                                                                  >  SRR4302533.3808474/1‑94 (MQ=60)
                             TGTTACGTACCGCGTCAAACAAGTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGG                                                                  <  SRR4302533.1494177/94‑1 (MQ=60)
                              GTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGT                                                                 >  SRR4302533.2675149/1‑94 (MQ=60)
                              GTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGT                                                                 <  SRR4302533.1915635/94‑1 (MQ=60)
                               TTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTT                                                                <  SRR4302533.2646104/94‑1 (MQ=60)
                                TACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTC                                                               >  SRR4302533.4057298/1‑94 (MQ=60)
                                  CGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCAT                                                             <  SRR4302533.9507/94‑1 (MQ=60)
                                   GTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATA                                                            >  SRR4302533.2610474/1‑94 (MQ=60)
                                   GTACCGCGTCAAACAATTTCGGCATGAAAGCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATA                                                            <  SRR4302533.1808069/94‑1 (MQ=60)
                                     ACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAG                                                          <  SRR4302533.3771404/94‑1 (MQ=60)
                                       CGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCC                                                        >  SRR4302533.4798724/1‑94 (MQ=60)
                                       CGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCC                                                        >  SRR4302533.5186098/1‑94 (MQ=60)
                                       CGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCC                                                        <  SRR4302533.1450428/94‑1 (MQ=60)
                                         CGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAG                                                      <  SRR4302533.3832775/94‑1 (MQ=60)
                                         CGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAG                                                      <  SRR4302533.2617728/94‑1 (MQ=60)
                                          GTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGA                                                     >  SRR4302533.5128187/1‑94 (MQ=60)
                                          GTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGA                                                     >  SRR4302533.5389980/1‑94 (MQ=60)
                                           GCAAACAATTTCGGCATGAAATCGAGGTATTCCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGAC                                                    <  SRR4302533.2985282/94‑1 (MQ=60)
                                            CAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACC                                                   >  SRR4302533.5224904/1‑94 (MQ=60)
                                            CAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACC                                                   >  SRR4302533.4117275/1‑94 (MQ=60)
                                            CAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACC                                                   <  SRR4302533.573736/94‑1 (MQ=60)
                                            CAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACC                                                   >  SRR4302533.1591281/1‑94 (MQ=60)
                                             AAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCT                                                  <  SRR4302533.2693310/94‑1 (MQ=60)
                                               ACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTT                                                <  SRR4302533.2888/94‑1 (MQ=60)
                                               ACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTT                                                >  SRR4302533.899925/1‑94 (MQ=60)
                                                 AATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTAC                                              >  SRR4302533.3961392/1‑94 (MQ=60)
                                                  ATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACC                                             >  SRR4302533.693035/1‑94 (MQ=60)
                                                    TTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTT                                           <  SRR4302533.6184422/94‑1 (MQ=60)
                                                      CGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTC                                         <  SRR4302533.6043907/94‑1 (MQ=60)
                                                      CGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTC                                         >  SRR4302533.1607974/1‑94 (MQ=60)
                                                      CGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTC                                         >  SRR4302533.5457128/1‑94 (MQ=60)
                                                      CGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTC                                         >  SRR4302533.5420248/1‑94 (MQ=60)
                                                      CGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTC                                         >  SRR4302533.4971878/1‑94 (MQ=60)
                                                       GGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCG                                        <  SRR4302533.840859/94‑1 (MQ=60)
                                                       GGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCG                                        <  SRR4302533.4169925/94‑1 (MQ=60)
                                                         CATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGAC                                      >  SRR4302533.4547541/1‑94 (MQ=60)
                                                         CATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGAC                                      >  SRR4302533.1312576/1‑94 (MQ=60)
                                                          ATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACA                                     >  SRR4302533.4289103/1‑94 (MQ=60)
                                                          ATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACA                                     >  SRR4302533.6333516/1‑94 (MQ=60)
                                                            GAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTCTACCTTTCGACATG                                   <  SRR4302533.82414/94‑1 (MQ=60)
                                                             AAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGC                                  <  SRR4302533.5631094/94‑1 (MQ=60)
                                                               ATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCG                                >  SRR4302533.2750554/1‑94 (MQ=60)
                                                                 CGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTA                              <  SRR4302533.259963/94‑1 (MQ=60)
                                                                 CGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTA                              >  SRR4302533.3722731/1‑94 (MQ=60)
                                                                  GAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAG                             <  SRR4302533.4833335/94‑1 (MQ=60)
                                                                  GAGGTATTTCTCCGTCGACCACAGCTGCTCGTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAG                             <  SRR4302533.1310649/94‑1 (MQ=60)
                                                                   AGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGG                            >  SRR4302533.2177280/1‑94 (MQ=60)
                                                                   AGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGG                            <  SRR4302533.6243018/94‑1 (MQ=60)
                                                                    GGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGT                           <  SRR4302533.5750772/94‑1 (MQ=60)
                                                                     GTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTG                          >  SRR4302533.5206436/1‑94 (MQ=60)
                                                                     GTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTG                          <  SRR4302533.95663/94‑1 (MQ=60)
                                                                      TATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGG                         <  SRR4302533.5098175/94‑1 (MQ=60)
                                                                          TCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTT                     >  SRR4302533.2469428/1‑94 (MQ=60)
                                                                           CTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTC                    <  SRR4302533.6251398/94‑1 (MQ=60)
                                                                           CTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTC                    <  SRR4302533.970195/94‑1 (MQ=60)
                                                                            TCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCA                   >  SRR4302533.5818910/1‑94 (MQ=60)
                                                                             CCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCAT                  >  SRR4302533.5840410/1‑94 (MQ=60)
                                                                             CCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCAT                  <  SRR4302533.690935/94‑1 (MQ=60)
                                                                               GTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAC                <  SRR4302533.511901/94‑1 (MQ=60)
                                                                               GTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAC                <  SRR4302533.2894071/94‑1 (MQ=60)
                                                                                 CGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCA              <  SRR4302533.2545349/94‑1 (MQ=60)
                                                                                  GACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCTGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAG             >  SRR4302533.820604/1‑94 (MQ=60)
                                                                                  GACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAG             >  SRR4302533.5790038/1‑94 (MQ=60)
                                                                                  GACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAG             >  SRR4302533.2868648/1‑94 (MQ=60)
                                                                                  GACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAG             >  SRR4302533.1360017/1‑94 (MQ=60)
                                                                                  GACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAG             >  SRR4302533.5148673/1‑94 (MQ=60)
                                                                                    CCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGG           >  SRR4302533.5667421/1‑94 (MQ=60)
                                                                                       CAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATT        <  SRR4302533.1215493/94‑1 (MQ=60)
                                                                                         GCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCC      <  SRR4302533.1717934/94‑1 (MQ=60)
                                                                                         GCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCC      <  SRR4302533.1507990/94‑1 (MQ=60)
                                                                                         GCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCC      >  SRR4302533.1794363/1‑94 (MQ=60)
                                                                                          CTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCG     >  SRR4302533.1503846/1‑94 (MQ=60)
                                                                                          CTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCG     >  SRR4302533.428637/1‑94 (MQ=60)
                                                                                            GCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCA   >  SRR4302533.3441315/1‑94 (MQ=60)
                                                                                             GTCTTCCGGCCACTGTCCTGTGGTTGCGGGTTCATAAGCCAGTGCTTTACCTTTCGACATGCGGTAGGTGGTTTTCATACCAGGGATTCCGCAC  <  SRR4302533.2816201/94‑1 (MQ=60)
                                                                                             CTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCAC  >  SRR4302533.1801619/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATGCAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCAC  >  NC_000913/1652533‑1652719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑