Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,872,006 T→A 94.3% S492C (AGC→TGC)  gyrB ← DNA gyrase, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,872,0060TA94.3% 611.9 / 21.2 194S492C (AGC→TGC) gyrBDNA gyrase, subunit B
Reads supporting (aligned to +/- strand):  ref base T (6/5);  new base A (83/100);  total (89/105)
Fisher's exact test for biased strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 200 total aligned reads displayed.

CTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCA  >  CP009273/3871862‑3872149
                                                                                                                                                |                                                                                                                                               
cTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCagtg                                                                                                                                              <  1:510336/148‑1 (MQ=255)
cTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCagtg                                                                                                                                              >  1:784981/1‑148 (MQ=255)
cTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCagtg                                                                                                                                              <  1:834559/148‑1 (MQ=255)
cTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCagtg                                                                                                                                              <  1:1562719/148‑1 (MQ=255)
 tttCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTC‑‑CATCGGTCATGATGATGATGCAGTGATa                                                                                                                                           <  2:2352906/148‑1 (MQ=255)
   tcttcACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATa                                                                                                                                           <  2:2812232/148‑1 (MQ=255)
      tcACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACg                                                                                                                                        >  2:2217589/1‑148 (MQ=255)
      tcACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACg                                                                                                                                        >  2:647159/1‑148 (MQ=255)
      tcACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACg                                                                                                                                        >  1:970043/1‑148 (MQ=255)
         cTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAg                                                                                                                                     <  1:2607806/148‑1 (MQ=255)
         cTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAg                                                                                                                                     >  2:1086308/1‑148 (MQ=255)
          tttGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGt                                                                                                                                    >  1:940395/1‑148 (MQ=255)
          tttGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGt                                                                                                                                    <  1:3140222/148‑1 (MQ=255)
          tttGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGt                                                                                                                                    <  2:2552967/148‑1 (MQ=255)
                  gcggcgGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCgg                                                                                                                            <  2:584048/148‑1 (MQ=255)
                  gcggcgGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCgg                                                                                                                            <  2:2193696/148‑1 (MQ=255)
                   cggcggCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCggg                                                                                                                           >  2:2461005/1‑148 (MQ=255)
                   cggcggCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCggg                                                                                                                           >  2:1395674/1‑148 (MQ=255)
                   cggcggCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCggg                                                                                                                           <  2:2833596/148‑1 (MQ=255)
                      cggcTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTg                                                                                                                        <  2:1574850/148‑1 (MQ=255)
                        gcTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCTGGTTGTa                                                                                                                      <  1:592622/148‑1 (MQ=255)
                         cTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTAc                                                                                                                     >  1:623894/1‑148 (MQ=255)
                          tGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACt                                                                                                                    <  1:2861813/148‑1 (MQ=255)
                             gCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGt                                                                                                                 <  2:3280969/148‑1 (MQ=255)
                                 tGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACg                                                                                                             >  2:2673514/1‑148 (MQ=255)
                                     gACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCg                                                                                                         <  1:1187647/148‑1 (MQ=255)
                                      aCGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGa                                                                                                        <  1:235350/148‑1 (MQ=255)
                                        gTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATa                                                                                                      <  1:2508286/148‑1 (MQ=255)
                                         tGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATAc                                                                                                     <  2:1808475/148‑1 (MQ=255)
                                           aCCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACca                                                                                                   >  2:2513511/1‑148 (MQ=255)
                                           aCCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGAATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACca                                                                                                   <  1:73665/148‑1 (MQ=255)
                                                 ttCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCa                                                                                             >  2:2344218/1‑148 (MQ=255)
                                                 ttCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCa                                                                                             <  1:813497/148‑1 (MQ=255)
                                                     aCGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAgcg                                                                                         >  2:1580541/1‑148 (MQ=255)
                                                       gATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGcgcg                                                                                       >  1:1719827/1‑148 (MQ=255)
                                                       gATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGcgcg                                                                                       >  1:969689/1‑148 (MQ=255)
                                                          ttCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAg                                                                                                                                     <  2:1855862/99‑1 (MQ=255)
                                                          ttCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAg                                                                                                                                     >  1:1856351/1‑99 (MQ=255)
                                                          ttCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTg                                                                                    <  2:1455589/148‑1 (MQ=255)
                                                              ggCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATaa                                                                                >  1:1549723/1‑148 (MQ=255)
                                                               gCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAg                                                                               <  2:663010/148‑1 (MQ=255)
                                                               gCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAg                                                                               >  1:884947/1‑148 (MQ=255)
                                                                 aTCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCg                                                                             <  2:1668760/148‑1 (MQ=255)
                                                                  tCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACCGCCAAGCGCGGTGATAAGCGt                                                                            <  2:3027880/148‑1 (MQ=255)
                                                                  tCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATCAGCGt                                                                            >  1:3196950/1‑148 (MQ=255)
                                                                          tAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACtt                                                                    >  1:3260930/1‑148 (MQ=255)
                                                                          tAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACtt                                                                    >  1:2722408/1‑148 (MQ=255)
                                                                          tAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACtt                                                                    >  1:460359/1‑148 (MQ=255)
                                                                           agaagaAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTc                                                                   >  2:2902208/1‑148 (MQ=255)
                                                                           agaagaAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTc                                                                   <  2:2857158/148‑1 (MQ=255)
                                                                           agaagaAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTc                                                                   >  1:1339572/1‑148 (MQ=255)
                                                                           agaagaAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTc                                                                   <  2:1031734/148‑1 (MQ=255)
                                                                           agaagaAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTc                                                                   <  2:238005/148‑1 (MQ=255)
                                                                            gaagaaGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTcc                                                                  <  2:3185269/148‑1 (MQ=255)
                                                                             aagaagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCt                                                                 >  1:419970/1‑148 (MQ=255)
                                                                              agaagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTg                                                                >  2:2819807/1‑148 (MQ=255)
                                                                                   gTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATACCGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaag                                                           <  2:2269317/148‑1 (MQ=255)
                                                                                   gTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaag                                                           <  2:3126483/148‑1 (MQ=255)
                                                                                   gTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaag                                                           <  1:111606/148‑1 (MQ=255)
                                                                                      aaCAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagaga                                                        <  1:66891/148‑1 (MQ=255)
                                                                                       aCAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagagag                                                       <  1:3057656/148‑1 (MQ=255)
                                                                                        cagcagCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGc                                                      <  2:2533589/148‑1 (MQ=255)
                                                                                           cagcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATc                                                   <  2:230640/148‑1 (MQ=255)
                                                                                            agcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCt                                                  <  2:2600256/148‑1 (MQ=255)
                                                                                            agcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCt                                                  <  2:968754/148‑1 (MQ=255)
                                                                                               gTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTAt                                               <  2:623876/148‑1 (MQ=255)
                                                                                               gTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTAt                                               <  2:337130/148‑1 (MQ=255)
                                                                                               gTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTAt                                               <  1:1416056/148‑1 (MQ=255)
                                                                                               gTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTAt                                               <  2:1985838/148‑1 (MQ=255)
                                                                                               gTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTAt                                               <  1:2616513/148‑1 (MQ=255)
                                                                                                 aCGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCg                                             >  2:1888593/1‑148 (MQ=255)
                                                                                                 aCGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCg                                             >  2:849395/1‑148 (MQ=255)
                                                                                                 aCGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCg                                             >  1:1655122/1‑148 (MQ=255)
                                                                                                    aaTGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAg                                          <  2:1415152/148‑1 (MQ=255)
                                                                                                      tgtgCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAgcg                                        <  1:1737263/148‑1 (MQ=255)
                                                                                                       gtgCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAgcgc                                       >  1:1955972/1‑148 (MQ=255)
                                                                                                         gCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAgcgcgc                                     <  1:1700407/148‑1 (MQ=255)
                                                                                                          cGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCt                                    <  1:2287269/148‑1 (MQ=255)
                                                                                                           gAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCtt                                   <  2:2945275/148‑1 (MQ=255)
                                                                                                           gAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCtt                                   <  2:1298148/148‑1 (MQ=255)
                                                                                                              ccGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTtct                                >  2:769740/1‑148 (MQ=255)
                                                                                                              ccGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTtct                                <  2:1571690/148‑1 (MQ=255)
                                                                                                                    aCGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGt                          >  1:2421961/1‑148 (MQ=255)
                                                                                                                     cGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGtt                         >  2:1887265/1‑148 (MQ=255)
                                                                                                                        ccGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAg                      >  1:2031031/1‑148 (MQ=255)
                                                                                                                         cGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAgg                     <  1:1142623/148‑1 (MQ=255)
                                                                                                                           cATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAt                   >  1:3260130/1‑148 (MQ=255)
                                                                                                                           cATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAt                   >  1:1228160/1‑148 (MQ=255)
                                                                                                                            aTCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAtt                  <  2:2778975/148‑1 (MQ=255)
                                                                                                                                gTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAc              <  2:784981/148‑1 (MQ=255)
                                                                                                                                 tCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAcc             >  2:2727543/1‑148 (MQ=255)
                                                                                                                                 tCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAcc             <  2:1726059/148‑1 (MQ=255)
                                                                                                                                  cATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAccc            >  2:1597022/1‑148 (MQ=255)
                                                                                                                                    tgatgatgatgCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCtt          >  1:3063223/1‑148 (MQ=255)
                                                                                                                                     gatgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTc         >  1:1012730/1‑148 (MQ=255)
                                                                                                                                     gatgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTc         <  1:1449604/148‑1 (MQ=255)
                                                                                                                                          tgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCgg    <  1:1460012/148‑1 (MQ=255)
                                                                                                                                          tgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCgg    >  1:2192026/1‑148 (MQ=255)
                                                                                                                                            atgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCa  >  1:1186307/1‑148 (MQ=255)
                                                                                                                                            atgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCa  <  2:324756/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCA  >  CP009273/3871862‑3872149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATC  >  CP009273/3871857‑3872154
                                                                                                                                                     |                                                                                                                                                    
CTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCA                                                                                                                                                      >  SRR19973070.1068434/1‑150 (MQ=60)
 TTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAATGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCAGCATCGGACATGATGATGATGCAG                                                                                                                                                     >  SRR19973070.1999281/1‑150 (MQ=60)
 TTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAG                                                                                                                                                     <  SRR19973070.2792310/150‑1 (MQ=60)
 TTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAG                                                                                                                                                     <  SRR19973070.826858/150‑1 (MQ=60)
   GCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTG                                                                                                                                                   >  SRR19973070.790161/1‑150 (MQ=60)
   GCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTG                                                                                                                                                   >  SRR19973070.790445/1‑150 (MQ=60)
     CTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGAT                                                                                                                                                 <  SRR19973070.513850/150‑1 (MQ=60)
     CTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGAT                                                                                                                                                 <  SRR19973070.1573718/150‑1 (MQ=60)
     CTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGAT                                                                                                                                                 <  SRR19973070.1573256/150‑1 (MQ=60)
     CTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGAT                                                                                                                                                 <  SRR19973070.840204/150‑1 (MQ=60)
      TTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATA                                                                                                                                                >  SRR19973070.2466126/1‑150 (MQ=60)
       TTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAA                                                                                                                                               >  SRR19973070.1468972/1‑150 (MQ=60)
        TCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAAC                                                                                                                                              <  SRR19973070.2413409/150‑1 (MQ=60)
         CTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACG                                                                                                                                             >  SRR19973070.976970/1‑150 (MQ=60)
         CTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACG                                                                                                                                             >  SRR19973070.975665/1‑150 (MQ=60)
         CTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACG                                                                                                                                             <  SRR19973070.3084532/150‑1 (MQ=60)
             ACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGT                                                                                                                                         >  SRR19973070.946932/1‑150 (MQ=60)
             ACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGT                                                                                                                                         >  SRR19973070.2670875/1‑150 (MQ=60)
             ACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGT                                                                                                                                         >  SRR19973070.947098/1‑150 (MQ=60)
              CTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTT                                                                                                                                        <  SRR19973070.2624831/150‑1 (MQ=60)
               TTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTT                                                                                                                                       <  SRR19973070.3160511/150‑1 (MQ=60)
                     CAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGG                                                                                                                                 <  SRR19973070.732985/150‑1 (MQ=60)
                         GGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTG                                                                                                                             >  SRR19973070.2014721/1‑150 (MQ=60)
                            GGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTAC                                                                                                                          >  SRR19973070.628285/1‑150 (MQ=60)
                            GGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTAC                                                                                                                          >  SRR19973070.628303/1‑150 (MQ=60)
                             GCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCTGGTTGTACT                                                                                                                         <  SRR19973070.596724/150‑1 (MQ=60)
                               TGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCG                                                                                                                       <  SRR19973070.1455467/150‑1 (MQ=60)
                               TGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCG                                                                                                                       <  SRR19973070.2880357/150‑1 (MQ=60)
                               TGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCG                                                                                                                       <  SRR19973070.1221559/150‑1 (MQ=60)
                               TGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCG                                                                                                                       >  SRR19973070.1680218/1‑150 (MQ=60)
                               TGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCG                                                                                                                       <  SRR19973070.2880360/150‑1 (MQ=60)
                               TGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCG                                                                                                                       <  SRR19973070.2889905/150‑1 (MQ=60)
                               TGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCG                                                                                                                       >  SRR19973070.831791/1‑150 (MQ=60)
                                   CGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCAC                                                                                                                   <  SRR19973070.426632/150‑1 (MQ=60)
                                   CGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAAAGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCAC                                                                                                                   >  SRR19973070.226184/1‑150 (MQ=60)
                                          GACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGAT                                                                                                            <  SRR19973070.1195994/150‑1 (MQ=60)
                                           ACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATA                                                                                                           <  SRR19973070.236995/150‑1 (MQ=60)
                                             GTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACC                                                                                                         <  SRR19973070.2524599/150‑1 (MQ=60)
                                             GTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACC                                                                                                         <  SRR19973070.859787/150‑1 (MQ=60)
                                              TGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCA                                                                                                        >  SRR19973070.1319063/1‑150 (MQ=60)
                                               GACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCAC                                                                                                       <  SRR19973070.1169383/150‑1 (MQ=60)
                                                ACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGAATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACA                                                                                                      <  SRR19973070.74461/150‑1 (MQ=60)
                                                     GTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAC                                                                                                 >  SRR19973070.3148658/1‑150 (MQ=60)
                                                      TTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAG                                                                                                <  SRR19973070.819066/150‑1 (MQ=60)
                                                         AACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGC                                                                                             >  SRR19973070.2558001/1‑150 (MQ=60)
                                                          ACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCG                                                                                            >  SRR19973070.976670/1‑150 (MQ=60)
                                                          ACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCG                                                                                            >  SRR19973070.1731723/1‑150 (MQ=60)
                                                          ACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCG                                                                                            >  SRR19973070.976610/1‑150 (MQ=60)
                                                           CGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGG                                                                                           >  SRR19973070.413646/1‑150 (MQ=60)
                                                             ATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTagatcggaagagcacacgtctgaactccagtcactgatctcacgatc                                                                                         >  SRR19973070.1868571/1‑103 (MQ=60)
                                                             ATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTagatcggaagagcacacgtctgaactccagtcactgatctcacgatc                                                                                         >  SRR19973070.1869063/1‑103 (MQ=60)
                                                                 CCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAA                                                                                     >  SRR19973070.1560622/1‑150 (MQ=60)
                                                                  CGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAG                                                                                    >  SRR19973070.891185/1‑150 (MQ=60)
                                                                     CATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATCAGCGT                                                                                 >  SRR19973070.3217638/1‑150 (MQ=60)
                                                                     CATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGT                                                                                 >  SRR19973070.1424832/1‑150 (MQ=60)
                                                                             GATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTT                                                                         >  SRR19973070.2739978/1‑150 (MQ=60)
                                                                             GATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTT                                                                         >  SRR19973070.3281834/1‑150 (MQ=60)
                                                                             GATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTT                                                                         >  SRR19973070.3281926/1‑150 (MQ=60)
                                                                             GATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTT                                                                         >  SRR19973070.463650/1‑150 (MQ=60)
                                                                              ATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTC                                                                        >  SRR19973070.1348780/1‑150 (MQ=60)
                                                                               TAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCC                                                                       >  SRR19973070.1006859/1‑150 (MQ=60)
                                                                                AGAAGAAGGTCAACAGCAGCGTACGACTGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCT                                                                      <  SRR19973070.2149786/150‑1 (MQ=60)
                                                                                AGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCT                                                                      >  SRR19973070.423032/1‑150 (MQ=60)
                                                                                   AGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAG                                                                   <  SRR19973070.1923361/150‑1 (MQ=60)
                                                                                        GTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAG                                                              <  SRR19973070.112640/150‑1 (MQ=60)
                                                                                           AACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGC                                                           <  SRR19973070.67632/150‑1 (MQ=60)
                                                                                            ACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCA                                                          <  SRR19973070.3077201/150‑1 (MQ=60)
                                                                                             CAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCAT                                                         <  SRR19973070.800102/150‑1 (MQ=60)
                                                                                                    GTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCG                                                  >  SRR19973070.1666531/1‑150 (MQ=60)
                                                                                                    GTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCG                                                  <  SRR19973070.2633582/150‑1 (MQ=60)
                                                                                                    GTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCG                                                  <  SRR19973070.2633228/150‑1 (MQ=60)
                                                                                                    GTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCG                                                  <  SRR19973070.1425755/150‑1 (MQ=60)
                                                                                                          ATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGC                                            >  SRR19973070.1969142/1‑150 (MQ=60)
                                                                                                           TGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCG                                           <  SRR19973070.1749298/150‑1 (MQ=60)
                                                                                                             TGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCT                                         <  SRR19973070.1656999/150‑1 (MQ=60)
                                                                                                              GCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTT                                        <  SRR19973070.1712123/150‑1 (MQ=60)
                                                                                                              GCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTT                                        <  SRR19973070.1712203/150‑1 (MQ=60)
                                                                                                               CGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTT                                       <  SRR19973070.2302481/150‑1 (MQ=60)
                                                                                                                    CGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGA                                  >  SRR19973070.2342646/1‑150 (MQ=60)
                                                                                                                       CGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGT                               >  SRR19973070.2437890/1‑150 (MQ=60)
                                                                                                                        GACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTT                              >  SRR19973070.1491869/1‑150 (MQ=60)
                                                                                                                           GTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAG                           >  SRR19973070.2044773/1‑150 (MQ=60)
                                                                                                                            TCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGG                          <  SRR19973070.559283/150‑1 (MQ=60)
                                                                                                                              CGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAT                        >  SRR19973070.1236759/1‑150 (MQ=60)
                                                                                                                              CGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAT                        >  SRR19973070.3281122/1‑150 (MQ=60)
                                                                                                                              CGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAT                        >  SRR19973070.3281107/1‑150 (MQ=60)
                                                                                                                              CGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAT                        <  SRR19973070.1150552/150‑1 (MQ=60)
                                                                                                                               GCATCGGTCATGATGAGGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGCTACCACAGCCAAGCGCGGTGATACGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAGT                       <  SRR19973070.629178/150‑1 (MQ=60)
                                                                                                                                     GTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTACTCGACGTTGAGGATTTTACCC                 >  SRR19973070.1098927/1‑150 (MQ=60)
                                                                                                                                       CATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTT               >  SRR19973070.3082841/1‑150 (MQ=60)
                                                                                                                                        ATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTC              >  SRR19973070.1019925/1‑150 (MQ=60)
                                                                                                                                         TGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCA             <  SRR19973070.468457/150‑1 (MQ=60)
                                                                                                                                         TGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCA             >  SRR19973070.113/1‑150 (MQ=60)
                                                                                                                                          GATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAG            <  SRR19973070.1459684/150‑1 (MQ=60)
                                                                                                                                          GATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAG            <  SRR19973070.174717/150‑1 (MQ=60)
                                                                                                                                             GATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGG         >  SRR19973070.2206502/1‑150 (MQ=60)
                                                                                                                                              ATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGC        >  SRR19973070.3197024/1‑150 (MQ=60)
                                                                                                                                               TGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCA       >  SRR19973070.1194646/1‑150 (MQ=60)
                                                                                                                                               TGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCA       >  SRR19973070.1195229/1‑150 (MQ=60)
                                                                                                                                               TGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCA       <  SRR19973070.1470199/150‑1 (MQ=60)
                                                                                                                                                    CAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATC  <  SRR19973070.1405081/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATC  >  CP009273/3871857‑3872154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑