Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,872,006 T→A 100% S492C (AGC→TGC)  gyrB ← DNA gyrase, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,872,0060TA100.0% 632.2 / NA 191S492C (AGC→TGC) gyrBDNA gyrase, subunit B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (88/103);  total (88/103)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 192 total aligned reads displayed.

CTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCA  >  CP009273/3871862‑3872149
                                                                                                                                                |                                                                                                                                               
  ttcttcACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGAt                                                                                                                                            >  1:903192/1‑148 (MQ=255)
   tcttcACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATa                                                                                                                                           <  1:3369222/148‑1 (MQ=255)
    cttcACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATaa                                                                                                                                          >  2:1350453/1‑148 (MQ=255)
         cTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAg                                                                                                                                     <  2:1817143/148‑1 (MQ=255)
          tttGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGt                                                                                                                                    <  1:2660920/148‑1 (MQ=255)
          tgtGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGGTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGt                                                                                                                                    <  2:70236/146‑1 (MQ=255)
           ttGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGtt                                                                                                                                   <  2:904370/148‑1 (MQ=255)
           ttGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGtt                                                                                                                                   <  1:2348377/148‑1 (MQ=255)
            tGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGttt                                                                                                                                  <  1:1966059/148‑1 (MQ=255)
            tGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAAAGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGttt                                                                                                                                  <  1:3327406/148‑1 (MQ=255)
             gTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTg                                                                                                                                 >  1:206575/1‑148 (MQ=255)
              gaCAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTgc                                                                                                                                <  1:403857/147‑2 (MQ=255)
               aCAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTc                                                                                                                               <  2:2427119/148‑1 (MQ=255)
               aCAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTc                                                                                                                               <  1:8304/148‑1 (MQ=255)
                  gcggcgGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCgg                                                                                                                            >  1:1187884/1‑148 (MQ=255)
                     gcggcTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGtt                                                                                                                         <  1:2135343/148‑1 (MQ=255)
                      cggcTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTg                                                                                                                        <  1:2388000/148‑1 (MQ=255)
                        gcTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTa                                                                                                                      >  1:506146/1‑148 (MQ=255)
                              cGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTc                                                                                                                <  1:417768/148‑1 (MQ=255)
                                   tAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGAACTCGTCACGAc                                                                                                           >  1:2293348/1‑148 (MQ=255)
                                    aGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGAcc                                                                                                          >  2:1266966/1‑148 (MQ=255)
                                      aCGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGa                                                                                                        >  1:2120341/1‑148 (MQ=255)
                                      aCGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGa                                                                                                        <  2:1012594/148‑1 (MQ=255)
                                       cGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGAt                                                                                                       <  2:2609610/148‑1 (MQ=255)
                                          gACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATAcc                                                                                                    <  1:2619627/148‑1 (MQ=255)
                                            ccGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACcac                                                                                                  <  2:660195/148‑1 (MQ=255)
                                             cgcgTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACcaca                                                                                                 <  1:3109922/148‑1 (MQ=255)
                                             cgcgTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACcaca                                                                                                 <  1:1362412/148‑1 (MQ=255)
                                                 ttCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCa                                                                                             <  2:2319432/148‑1 (MQ=255)
                                                  tCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCaa                                                                                            >  1:1126786/1‑148 (MQ=255)
                                                   cAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAg                                                                                           >  2:1795482/1‑148 (MQ=255)
                                                      cGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAgcgc                                                                                        >  1:3019962/1‑148 (MQ=255)
                                                        aTTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCgg                                                                                      >  1:948361/1‑148 (MQ=255)
                                                        aTTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCgg                                                                                      <  2:2738510/148‑1 (MQ=255)
                                                        aTTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCgg                                                                                      <  2:982403/148‑1 (MQ=255)
                                                               gCATCTGCCGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAg                                                                               >  2:2207886/1‑148 (MQ=255)
                                                               gCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAg                                                                               >  2:1412913/1‑148 (MQ=255)
                                                                 aTCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCg                                                                             <  1:812772/148‑1 (MQ=255)
                                                                 aTCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCg                                                                             <  1:940538/148‑1 (MQ=255)
                                                                  tCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGt                                                                            >  1:626834/1‑148 (MQ=255)
                                                                         aTAGAAGAAGGTCAACAGCAGCGTACGGATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACt                                                                     >  2:620920/1‑148 (MQ=255)
                                                                         aTAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACt                                                                     <  2:1303223/148‑1 (MQ=255)
                                                                          tAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACtt                                                                    >  1:1846634/1‑148 (MQ=255)
                                                                            gaagaaGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTcc                                                                  <  2:2494070/148‑1 (MQ=255)
                                                                            gaagaaGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTcc                                                                  <  2:1579958/148‑1 (MQ=255)
                                                                            gaagaaGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTcc                                                                  <  2:3322106/148‑1 (MQ=255)
                                                                             aagaagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCt                                                                 >  1:2171479/1‑148 (MQ=255)
                                                                              agaagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTg                                                                <  2:3201870/148‑1 (MQ=255)
                                                                                  ggTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaa                                                            >  2:1761015/1‑148 (MQ=255)
                                                                                  ggTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaa                                                            >  2:801386/1‑148 (MQ=255)
                                                                                      aaCAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagaga                                                        <  1:1929256/148‑1 (MQ=255)
                                                                                      aaCAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagaga                                                        <  2:2778896/148‑1 (MQ=255)
                                                                                        cagcagCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGc                                                      >  1:1636651/1‑148 (MQ=255)
                                                                                           cagcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATc                                                   <  1:1795482/148‑1 (MQ=255)
                                                                                             gcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCtt                                                 <  2:2405037/148‑1 (MQ=255)
                                                                                              cGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTa                                                <  2:1651094/148‑1 (MQ=255)
                                                                                              cGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTa                                                <  1:1309238/148‑1 (MQ=255)
                                                                                                tACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATc                                              >  1:1696648/1‑148 (MQ=255)
                                                                                                 aCGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCg                                             >  2:1952548/1‑148 (MQ=255)
                                                                                                  cGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGa                                            <  1:1600593/148‑1 (MQ=255)
                                                                                                   gAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGaa                                           <  1:429468/148‑1 (MQ=255)
                                                                                                     aTGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAgc                                         >  2:3193209/1‑148 (MQ=255)
                                                                                                     aTGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAgc                                         >  1:2355496/1‑148 (MQ=255)
                                                                                                     aTGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAgc                                         >  1:3512598/1‑148 (MQ=255)
                                                                                                     aTGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAgc                                         >  1:3512602/1‑148 (MQ=255)
                                                                                                             gCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTtc                                 <  2:1906883/148‑1 (MQ=255)
                                                                                                              ccGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTtct                                >  1:986109/1‑148 (MQ=255)
                                                                                                                   gACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACg                           <  1:657581/148‑1 (MQ=255)
                                                                                                                    aCGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGt                          >  2:90141/1‑148 (MQ=255)
                                                                                                                    aCGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGt                          <  1:2034540/148‑1 (MQ=255)
                                                                                                                    aCGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGt                          <  1:260718/148‑1 (MQ=255)
                                                                                                                    aCGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGt                          <  1:2560894/148‑1 (MQ=255)
                                                                                                                     cGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGtt                         >  1:2150080/1‑148 (MQ=255)
                                                                                                                       tCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGa                       <  2:2064567/148‑1 (MQ=255)
                                                                                                                       tCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGa                       >  1:1471006/1‑148 (MQ=255)
                                                                                                                        ccGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCt                                                  >  2:425867/1‑120 (MQ=255)
                                                                                                                          gCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGa                    <  2:511241/148‑1 (MQ=255)
                                                                                                                          gCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGa                    <  2:1104641/148‑1 (MQ=255)
                                                                                                                          gCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGa                    >  2:1082455/1‑148 (MQ=255)
                                                                                                                           cATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTCTCGAAGCGCGCTTTCTCGAGGTTGAGGAt                   >  2:2986612/1‑148 (MQ=255)
                                                                                                                           cATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAt                   >  2:373303/1‑148 (MQ=255)
                                                                                                                               ggTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTTAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTa               <  2:1126786/148‑1 (MQ=255)
                                                                                                                               ggTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTTAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTa               <  2:3068444/148‑1 (MQ=255)
                                                                                                                               ggTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTa               <  1:741322/148‑1 (MQ=255)
                                                                                                                               ggTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTa               <  1:804237/148‑1 (MQ=255)
                                                                                                                                gTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAc              <  2:1080467/148‑1 (MQ=255)
                                                                                                                                gTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAc              <  2:1285973/148‑1 (MQ=255)
                                                                                                                                  cATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAccc            >  2:2960886/1‑148 (MQ=255)
                                                                                                                                  cATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAccc            >  2:1211022/1‑148 (MQ=255)
                                                                                                                                  cATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAccc            <  2:1389729/148‑1 (MQ=255)
                                                                                                                                    tgatgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCtt          <  2:2249291/148‑1 (MQ=255)
                                                                                                                                       tgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAg       <  2:117478/148‑1 (MQ=255)
                                                                                                                                        gatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGc      >  2:660652/1‑148 (MQ=255)
                                                                                                                                         atgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCg     >  1:2023487/1‑148 (MQ=255)
                                                                                                                                         atgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCg     >  1:2821403/1‑148 (MQ=255)
                                                                                                                                          tgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCgg    >  1:396780/1‑148 (MQ=255)
                                                                                                                                            atgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAt                   >  2:3359437/1‑131 (MQ=255)
                                                                                                                                            atgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAt                   >  2:3359496/1‑131 (MQ=255)
                                                                                                                                            atgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAt                   <  1:3359437/131‑1 (MQ=255)
                                                                                                                                            atgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCa  >  1:2162693/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCA  >  CP009273/3871862‑3872149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCG  >  CP009273/3871857‑3872155
                                                                                                                                                     |                                                                                                                                                     
CTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCA                                                                                                                                                       >  SRR19973065.1632855/1‑150 (MQ=60)
   GCCTTTCTTCACTGTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTG                                                                                                                                                    <  SRR19973065.2724065/150‑1 (MQ=60)
     CTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGAT                                                                                                                                                  <  SRR19973065.2931503/150‑1 (MQ=60)
     CTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGAT                                                                                                                                                  >  SRR19973065.909003/1‑150 (MQ=60)
        TCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAAC                                                                                                                                               <  SRR19973065.3389828/150‑1 (MQ=60)
         CTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACG                                                                                                                                              >  SRR19973065.2241570/1‑150 (MQ=60)
             ACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGT                                                                                                                                          <  SRR19973065.901486/150‑1 (MQ=60)
               TTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTT                                                                                                                                        <  SRR19973065.2677379/150‑1 (MQ=60)
                TTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTG                                                                                                                                       >  SRR19973065.208144/1‑150 (MQ=60)
                TTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTG                                                                                                                                       <  SRR19973065.2363011/150‑1 (MQ=60)
                 TGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGT                                                                                                                                      <  SRR19973065.1978891/150‑1 (MQ=60)
                 TGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAAAGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGT                                                                                                                                      <  SRR19973065.3347711/150‑1 (MQ=60)
                   TACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCC                                                                                                                                    <  SRR19973065.2036010/150‑1 (MQ=60)
                   GACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGCCC                                                                                                                                    <  SRR19973065.406684/150‑1 (MQ=60)
                    ACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCG                                                                                                                                   <  SRR19973065.8307/150‑1 (MQ=60)
                    ACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCG                                                                                                                                   <  SRR19973065.8433/150‑1 (MQ=60)
                     CAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGG                                                                                                                                  >  SRR19973065.1195395/1‑150 (MQ=60)
                          GCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGT                                                                                                                             <  SRR19973065.2149084/150‑1 (MQ=60)
                          GCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGT                                                                                                                             <  SRR19973065.2725818/150‑1 (MQ=60)
                           CGGCTGAGCGATGTAGACGTGACCGCGTTCTACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTA                                                                                                                            <  SRR19973065.916230/150‑1 (MQ=60)
                           CGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTA                                                                                                                            <  SRR19973065.2402984/150‑1 (MQ=60)
                           CGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTA                                                                                                                            <  SRR19973065.2402979/150‑1 (MQ=60)
                           CGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTA                                                                                                                            <  SRR19973065.1308112/150‑1 (MQ=60)
                           CGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTA                                                                                                                            >  SRR19973065.509519/1‑150 (MQ=60)
                           CGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTA                                                                                                                            <  SRR19973065.617642/150‑1 (MQ=60)
                                   CGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCAC                                                                                                                    >  SRR19973065.1969163/1‑150 (MQ=60)
                                   CGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCAC                                                                                                                    <  SRR19973065.420688/150‑1 (MQ=60)
                                   CGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCAC                                                                                                                    >  SRR19973065.2351461/1‑150 (MQ=60)
                                      TGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGAACTCGTCACGAC                                                                                                                 >  SRR19973065.2307798/1‑150 (MQ=60)
                                         AGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGA                                                                                                              >  SRR19973065.2133968/1‑150 (MQ=60)
                                         AGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGA                                                                                                              >  SRR19973065.2133989/1‑150 (MQ=60)
                                         AGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGA                                                                                                              >  SRR19973065.2134014/1‑150 (MQ=60)
                                               GACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCAC                                                                                                        <  SRR19973065.2635844/150‑1 (MQ=60)
                                               GACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCAC                                                                                                        <  SRR19973065.2636603/150‑1 (MQ=60)
                                                  CGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGC                                                                                                     <  SRR19973065.3128881/150‑1 (MQ=60)
                                                  CGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGC                                                                                                     <  SRR19973065.1371432/150‑1 (MQ=60)
                                                     GTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAA                                                                                                  >  SRR19973065.3087201/1‑150 (MQ=60)
                                                     GTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAA                                                                                                  >  SRR19973065.1134115/1‑150 (MQ=60)
                                                         AACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGC                                                                                              >  SRR19973065.3038465/1‑150 (MQ=60)
                                                           CGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGG                                                                                            >  SRR19973065.954628/1‑150 (MQ=60)
                                                            GATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGT                                                                                           <  SRR19973065.1453215/150‑1 (MQ=60)
                                                              TTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGA                                                                                         <  SRR19973065.488214/150‑1 (MQ=60)
                                                                  CGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAG                                                                                     >  SRR19973065.400108/1‑150 (MQ=60)
                                                                  CGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAG                                                                                     >  SRR19973065.3224717/1‑150 (MQ=60)
                                                                     CATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGT                                                                                  >  SRR19973065.630908/1‑150 (MQ=60)
                                                                      ATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTC                                                                                 <  SRR19973065.947549/150‑1 (MQ=60)
                                                                      ATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTC                                                                                 <  SRR19973065.946749/150‑1 (MQ=60)
                                                                      ATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTC                                                                                 <  SRR19973065.818131/150‑1 (MQ=60)
                                                                             GATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTT                                                                          >  SRR19973065.1858684/1‑150 (MQ=60)
                                                                             GATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTT                                                                          >  SRR19973065.1858801/1‑150 (MQ=60)
                                                                               TAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGGGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCC                                                                        >  SRR19973065.1667643/1‑150 (MQ=60)
                                                                                AGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCT                                                                       >  SRR19973065.2185413/1‑150 (MQ=60)
                                                                                AGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCT                                                                       >  SRR19973065.2185369/1‑150 (MQ=60)
                                                                                           AACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGC                                                            >  SRR19973065.1647641/1‑150 (MQ=60)
                                                                                           AACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGC                                                            <  SRR19973065.1941897/150‑1 (MQ=60)
                                                                                            acagccccctacgacggcGCGAGCCCTCGACGTCCCCCTCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCA                                                           <  SRR19973065.3261147/132‑1 (MQ=60)
                                                                                             CAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCAT                                                          >  SRR19973065.1721149/1‑150 (MQ=60)
                                                                                                CAGCGTACGATTGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTT                                                       <  SRR19973065.1807266/150‑1 (MQ=60)
                                                                                                 agttcagacgtgtgctcttccgatctGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTA                                                      <  SRR19973065.428829/124‑1 (MQ=60)
                                                                                                   CGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATC                                                    >  SRR19973065.1348911/1‑150 (MQ=60)
                                                                                                   CGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATC                                                    <  SRR19973065.1317883/150‑1 (MQ=60)
                                                                                                   CGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATC                                                    >  SRR19973065.1707970/1‑150 (MQ=60)
                                                                                                    GTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCG                                                   >  SRR19973065.645693/1‑150 (MQ=60)
                                                                                                       CGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAG                                                >  SRR19973065.1899380/1‑150 (MQ=60)
                                                                                                       CGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAG                                                <  SRR19973065.205657/150‑1 (MQ=60)
                                                                                                       CGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAG                                                <  SRR19973065.1611288/150‑1 (MQ=60)
                                                                                                        GAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGC                                               >  SRR19973065.2369047/1‑150 (MQ=60)
                                                                                                        GAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGC                                               <  SRR19973065.432449/150‑1 (MQ=60)
                                                                                                        GAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGC                                               >  SRR19973065.3533901/1‑150 (MQ=60)
                                                                                                        GAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGC                                               >  SRR19973065.3533898/1‑150 (MQ=60)
                                                                                                        GAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGC                                               >  SRR19973065.3533897/1‑150 (MQ=60)
                                                                                                        GAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGC                                               >  SRR19973065.2370142/1‑150 (MQ=60)
                                                                                                         AATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCG                                              >  SRR19973065.1488070/1‑150 (MQ=60)
                                                                                                          ATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGC                                             >  SRR19973065.341521/1‑150 (MQ=60)
                                                                                                                 AGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCT                                      >  SRR19973065.992660/1‑150 (MQ=60)
                                                                                                                 AGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCT                                      >  SRR19973065.992609/1‑150 (MQ=60)
                                                                                                                  GCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTC                                     <  SRR19973065.73762/150‑1 (MQ=60)
                                                                                                                     GTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGAC                                  >  SRR19973065.1620469/1‑150 (MQ=60)
                                                                                                                        GACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTT                               >  SRR19973065.2163908/1‑150 (MQ=60)
                                                                                                                        GACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTT                               >  SRR19973065.2153823/1‑150 (MQ=60)
                                                                                                                        GACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTT                               <  SRR19973065.661922/150‑1 (MQ=60)
                                                                                                                         ACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTG                              <  SRR19973065.2047586/150‑1 (MQ=60)
                                                                                                                         ACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTG                              <  SRR19973065.2576906/150‑1 (MQ=60)
                                                                                                                         ACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTG                              <  SRR19973065.262517/150‑1 (MQ=60)
                                                                                                                          CGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGA                             >  SRR19973065.1480433/1‑150 (MQ=60)
                                                                                                                              CGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAT                         <  SRR19973065.2619221/150‑1 (MQ=60)
                                                                                                                                gtgctcttccgatctTGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGGGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTT                       <  SRR19973065.3379978/135‑1 (MQ=60)
                                                                                                                                gtgctcttccgatctTGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTT                       <  SRR19973065.3380037/135‑1 (MQ=60)
                                                                                                                                 ATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTT                      <  SRR19973065.812654/150‑1 (MQ=60)
                                                                                                                                    GGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACC                   <  SRR19973065.746283/150‑1 (MQ=60)
                                                                                                                                    GGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACC                   <  SRR19973065.809553/150‑1 (MQ=60)
                                                                                                                                    GGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACC                   <  SRR19973065.808637/150‑1 (MQ=60)
                                                                                                                                      TCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCT                 <  SRR19973065.2376601/150‑1 (MQ=60)
                                                                                                                                         TGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCA              <  SRR19973065.3353785/150‑1 (MQ=60)
                                                                                                                                            TGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCG           >  SRR19973065.2838833/1‑150 (MQ=60)
                                                                                                                                            TGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCG           >  SRR19973065.2036498/1‑150 (MQ=60)
                                                                                                                                             GATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGG          >  SRR19973065.399550/1‑150 (MQ=60)
                                                                                                                                               TGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCA        >  SRR19973065.396158/1‑150 (MQ=60)
                                                                                                                                               TGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCA        >  SRR19973065.2176591/1‑150 (MQ=60)
                                                                                                                                               TGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCA        >  SRR19973065.2176585/1‑150 (MQ=60)
                                                                                                                                                 ATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGA      >  SRR19973065.916082/1‑150 (MQ=60)
                                                                                                                                                     AGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCG  <  SRR19973065.2695779/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCG  >  CP009273/3871857‑3872155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑