Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,880,731 G→T 100% D182Y (GAT→TAT)  mnmE → tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,880,7310GT97.2% 677.6 / 8.4 216D182Y (GAT→TAT) mnmEtRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase
Reads supporting (aligned to +/- strand):  ref base G (6/0);  new base T (104/106);  total (110/106)
Fisher's exact test for biased strand distribution p-value = 2.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 216 total aligned reads displayed.

CGACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCGATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAAT  >  CP009273/3880586‑3880875
                                                                                                                                                 |                                                                                                                                                
cGACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTAt                                                                                                                                                <  1:1936026/148‑1 (MQ=255)
   cGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTc                                                                                                                                             <  2:3411599/148‑1 (MQ=255)
       aGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCctct                                                                                                                                         <  2:1242189/148‑1 (MQ=255)
        gTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCtctc                                                                                                                                        <  2:1834819/148‑1 (MQ=255)
        gTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCtctc                                                                                                                                        <  2:2233052/148‑1 (MQ=255)
        gTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCtctc                                                                                                                                        <  1:1692023/148‑1 (MQ=255)
          tCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCg                                                                                                                                      <  1:1903682/148‑1 (MQ=255)
               aCAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGa                                                                                                                                 <  1:3414290/148‑1 (MQ=255)
                cAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGaa                                                                                                                                >  2:2352537/1‑148 (MQ=255)
                   gcggcCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGaaaaa                                                                                                                             >  2:2499358/1‑148 (MQ=255)
                      gcCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTg                                                                                                                          >  2:1158210/1‑148 (MQ=255)
                          gTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGc                                                                                                                      <  1:2941302/148‑1 (MQ=255)
                             cGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCa                                                                                                                   >  2:2890783/1‑148 (MQ=255)
                              ggCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAg                                                                                                                  <  2:2120669/148‑1 (MQ=255)
                              ggCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTGACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATGTCCTCTCCGACGGAAAAATTGAAGCCCAg                                                                                                                  <  1:3152376/148‑1 (MQ=255)
                               gCACTTAACTCGCTACAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGc                                                                                                                 >  2:789519/1‑148 (MQ=255)
                                cACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCt                                                                                                                >  2:2719911/1‑148 (MQ=255)
                                  cTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCa                                                                                                              <  1:353145/148‑1 (MQ=255)
                                  cTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCa                                                                                                              <  1:2121159/148‑1 (MQ=255)
                                   ttAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCaa                                                                                                             <  1:1994243/148‑1 (MQ=255)
                                     aaCTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATg                                                                                                           <  2:91286/148‑1 (MQ=255)
                                      aCTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGa                                                                                                          <  1:3141419/148‑1 (MQ=255)
                                       cTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGAc                                                                                                         >  2:3472225/1‑148 (MQ=255)
                                        tCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACg                                                                                                        >  2:3106811/1‑148 (MQ=255)
                                            tGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTAt                                                                                                    >  2:1168692/1‑148 (MQ=255)
                                             gCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTAtt                                                                                                   >  2:1141942/1‑148 (MQ=255)
                                                  gcgcATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGa                                                                                              <  1:3445619/148‑1 (MQ=255)
                                                    gcATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATc                                                                                            <  2:276877/148‑1 (MQ=255)
                                                    gcATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATc                                                                                            <  2:2647825/148‑1 (MQ=255)
                                                     cATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCt                                                                                           >  2:3079628/1‑148 (MQ=255)
                                                     cATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCt                                                                                           >  1:596411/1‑148 (MQ=255)
                                                      aTTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCtt                                                                                          >  2:1637166/1‑148 (MQ=255)
                                                      aTTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCtt                                                                                          >  2:786386/1‑148 (MQ=255)
                                                        tctcCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGa                                                                                        <  1:1233353/148‑1 (MQ=255)
                                                            cGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCa                                                                                    <  2:1395143/148‑1 (MQ=255)
                                                            cGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCa                                                                                    <  2:3414840/148‑1 (MQ=255)
                                                               aCGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAgtg                                                                                 >  2:101760/1‑148 (MQ=255)
                                                               aCGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAgtg                                                                                 >  2:2604154/1‑148 (MQ=255)
                                                                cGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAgtgc                                                                                >  2:524889/1‑148 (MQ=255)
                                                                  ggTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAgtgcgt                                                                              >  1:694397/1‑148 (MQ=255)
                                                                       atcatcTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGa                                                                         >  2:2582372/1‑148 (MQ=255)
                                                                          atcTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGc                                                                      <  1:2725708/148‑1 (MQ=255)
                                                                           tcTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCa                                                                     >  1:1417755/1‑148 (MQ=255)
                                                                           tcTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCa                                                                     <  1:1565983/148‑1 (MQ=255)
                                                                           tcTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCa                                                                     >  2:1504923/1‑148 (MQ=255)
                                                                              ggTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGt                                                                  <  2:612767/148‑1 (MQ=255)
                                                                              ggTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGt                                                                  <  2:727057/148‑1 (MQ=255)
                                                                                tAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCa                                                                <  1:2275489/148‑1 (MQ=255)
                                                                                tAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCa                                                                >  2:3391529/1‑148 (MQ=255)
                                                                                    aGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGt                                                            <  1:1168692/148‑1 (MQ=255)
                                                                                      cccTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAg                                                          >  1:544480/1‑148 (MQ=255)
                                                                                      cccTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAg                                                          >  1:1414544/1‑148 (MQ=255)
                                                                                       ccTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGt                                                         <  2:1534508/148‑1 (MQ=255)
                                                                                       ccTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGt                                                         <  2:186499/148‑1 (MQ=255)
                                                                                        cTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGtt                                                        <  1:2357916/148‑1 (MQ=255)
                                                                                           aCCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTttgt                                                     >  2:1545978/1‑148 (MQ=255)
                                                                                            cccACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTttgtt                                                    <  1:1125563/148‑1 (MQ=255)
                                                                                            cccACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTttgtt                                                    <  1:1125396/148‑1 (MQ=255)
                                                                                             ccACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTttgttg                                                   <  1:2604259/148‑1 (MQ=255)
                                                                                               aCTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTgcg                                                 >  1:3060933/1‑148 (MQ=255)
                                                                                                cTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTgcgc                                                >  1:510168/1‑148 (MQ=255)
                                                                                                  tGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGa                                              <  2:1356012/148‑1 (MQ=255)
                                                                                                  tGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGa                                              >  1:299339/1‑148 (MQ=255)
                                                                                                        tttACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGAt                                        <  2:2008460/148‑1 (MQ=255)
                                                                                                         ttACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATg                                       <  1:1850479/148‑1 (MQ=255)
                                                                                                         ttACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCGATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTCGTTTGTTGCGCGAAGGGATg                                       >  2:1705751/1‑148 (MQ=255)
                                                                                                             gTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAg                                   <  2:578103/148‑1 (MQ=255)
                                                                                                               cGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAgtg                                 >  1:1738933/1‑148 (MQ=255)
                                                                                                                gAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAgtgg                                <  2:3026798/148‑1 (MQ=255)
                                                                                                                 aaGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAgtggt                               >  1:1331177/1‑148 (MQ=255)
                                                                                                                 aaGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAgtggt                               >  1:1464435/1‑148 (MQ=255)
                                                                                                                 aaGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAgtggt                               >  2:3307792/1‑148 (MQ=255)
                                                                                                                   gCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGa                             <  1:2719911/148‑1 (MQ=255)
                                                                                                                    cGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGAt                            >  2:142521/1‑148 (MQ=255)
                                                                                                                     ggCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGAtt                           <  2:519832/148‑1 (MQ=255)
                                                                                                                       cAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGc                         >  1:1023257/1‑148 (MQ=255)
                                                                                                                       cAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGc                         >  1:1023219/1‑148 (MQ=255)
                                                                                                                       cAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGc                         >  2:1380109/1‑148 (MQ=255)
                                                                                                                        aaTTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGcc                        <  1:3309845/148‑1 (MQ=255)
                                                                                                                         aTTGATTTCCCCGATGAAGAGATCGATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCg                       >  2:1882131/1‑148 (MQ=255)
                                                                                                                         aTTGATTTCCCCGATGAAGAGATCGATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCg                       >  2:1196424/1‑148 (MQ=255)
                                                                                                                          ttGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCgg                      <  1:2466930/148‑1 (MQ=255)
                                                                                                                           tGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGa                     <  2:2982027/148‑1 (MQ=255)
                                                                                                                           tGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGa                     >  2:542383/1‑148 (MQ=255)
                                                                                                                            gATTTCGCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGAc                    <  1:2361121/148‑1 (MQ=255)
                                                                                                                             aTTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACg                   <  2:2682712/148‑1 (MQ=255)
                                                                                                                             aTTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACg                   <  2:322410/148‑1 (MQ=255)
                                                                                                                               ttCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTc                 >  1:802407/1‑148 (MQ=255)
                                                                                                                                tCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTcc                <  1:2900331/148‑1 (MQ=255)
                                                                                                                                   ccGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTaa             >  2:3010895/1‑148 (MQ=255)
                                                                                                                                    cGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAAc            >  1:2687437/1‑148 (MQ=255)
                                                                                                                                    cGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAAc            >  2:3151085/1‑148 (MQ=255)
                                                                                                                                    cGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAAc            >  2:3150601/1‑148 (MQ=255)
                                                                                                                                     gATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACg           >  1:2349617/1‑148 (MQ=255)
                                                                                                                                      aTGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGc          >  2:1841010/1‑148 (MQ=255)
                                                                                                                                       tGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGcc         >  2:2276406/1‑148 (MQ=255)
                                                                                                                                         aaGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCgg       >  1:150677/1‑148 (MQ=255)
                                                                                                                                          agagATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGt      <  2:3478188/148‑1 (MQ=255)
                                                                                                                                           gagaTCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTa     >  1:1135736/1‑148 (MQ=255)
                                                                                                                                              aTCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAAt  >  2:1935852/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCGATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAAT  >  CP009273/3880586‑3880875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATCGACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCGATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAATCGAG  >  CP009273/3880583‑3880879
                                                                                                                                                    |                                                                                                                                                    
TATCGACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTA                                                                                                                                                     <  SRR19973065.737450/150‑1 (MQ=60)
 ATCGACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTAT                                                                                                                                                    <  SRR19973065.117990/150‑1 (MQ=60)
   CGACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTT                                                                                                                                                  <  SRR19973065.1948699/150‑1 (MQ=60)
    GACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTC                                                                                                                                                 <  SRR19973065.1257010/150‑1 (MQ=60)
    GACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTC                                                                                                                                                 <  SRR19973065.1256936/150‑1 (MQ=60)
        CCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCT                                                                                                                                             <  SRR19973065.316351/150‑1 (MQ=60)
           GTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCG                                                                                                                                          <  SRR19973065.1703319/150‑1 (MQ=60)
             TCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGAC                                                                                                                                        <  SRR19973065.1916229/150‑1 (MQ=60)
             TCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGAC                                                                                                                                        <  SRR19973065.1916192/150‑1 (MQ=60)
               GGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGG                                                                                                                                      <  SRR19973065.1439025/150‑1 (MQ=60)
               GGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGG                                                                                                                                      <  SRR19973065.1908456/150‑1 (MQ=60)
                 AACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAA                                                                                                                                    <  SRR19973065.2178741/150‑1 (MQ=60)
                  ACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAA                                                                                                                                   <  SRR19973065.3435154/150‑1 (MQ=60)
                  ACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAA                                                                                                                                   <  SRR19973065.3435257/150‑1 (MQ=60)
                      GCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATT                                                                                                                               <  SRR19973065.3478010/150‑1 (MQ=60)
                      GCGGCCCGTTCGACACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAAGT                                                                                                                               <  SRR19973065.3520307/150‑1 (MQ=60)
                        GGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGA                                                                                                                             >  SRR19973065.1726648/1‑150 (MQ=60)
                         GCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAA                                                                                                                            >  SRR19973065.27397/1‑150 (MQ=60)
                             GTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCC                                                                                                                        >  SRR19973065.2424967/1‑150 (MQ=60)
                             GTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCC                                                                                                                        <  SRR19973065.2959158/150‑1 (MQ=60)
                                 GGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTGACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATGTCCTCTCCGACGGAAAAATTGAAGCCCAGCT                                                                                                                    <  SRR19973065.3171501/150‑1 (MQ=60)
                                     CTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAAT                                                                                                                <  SRR19973065.355620/150‑1 (MQ=60)
                                     CTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAAT                                                                                                                <  SRR19973065.932557/150‑1 (MQ=60)
                                     CTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAAT                                                                                                                >  SRR19973065.198622/1‑150 (MQ=60)
                                     CTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAAT                                                                                                                <  SRR19973065.2134814/150‑1 (MQ=60)
                                      TTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATG                                                                                                               <  SRR19973065.2007168/150‑1 (MQ=60)
                                      TTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATG                                                                                                               <  SRR19973065.1483937/150‑1 (MQ=60)
                                        AACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGAC                                                                                                             <  SRR19973065.2539406/150‑1 (MQ=60)
                                         ACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACG                                                                                                            <  SRR19973065.3160514/150‑1 (MQ=60)
                                                     GCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATC                                                                                                <  SRR19973065.3466624/150‑1 (MQ=60)
                                                      CGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCT                                                                                               <  SRR19973065.1141527/150‑1 (MQ=60)
                                                      CGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCT                                                                                               >  SRR19973065.600112/1‑150 (MQ=60)
                                                       GCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTT                                                                                              <  SRR19973065.1123957/150‑1 (MQ=60)
                                                           TCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATG                                                                                          <  SRR19973065.1241430/150‑1 (MQ=60)
                                                                 CACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGC                                                                                    >  SRR19973065.1697392/1‑150 (MQ=60)
                                                                   CGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGT                                                                                  >  SRR19973065.699062/1‑150 (MQ=60)
                                                                   CGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGT                                                                                  >  SRR19973065.699021/1‑150 (MQ=60)
                                                                          ATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAG                                                                           <  SRR19973065.490927/150‑1 (MQ=60)
                                                                            CATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCA                                                                         >  SRR19973065.1427011/1‑150 (MQ=60)
                                                                            CATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCA                                                                         >  SRR19973065.1427004/1‑150 (MQ=60)
                                                                             ATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCAC                                                                        <  SRR19973065.2742663/150‑1 (MQ=60)
                                                                             ATCTGGTAGAAGCCCTCACCCACTTGCGCATGTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCAC                                                                        <  SRR19973065.2742612/150‑1 (MQ=60)
                                                                              TCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACG                                                                       <  SRR19973065.1576393/150‑1 (MQ=60)
                                                                              TCTGGTAGAAGACCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACG                                                                       <  SRR19973065.2089992/150‑1 (MQ=60)
                                                                                   TAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGG                                                                  <  SRR19973065.2289848/150‑1 (MQ=60)
                                                                                      AAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTA                                                               <  SRR19973065.3011497/150‑1 (MQ=60)
                                                                                      AAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTA                                                               >  SRR19973065.2945863/1‑150 (MQ=60)
                                                                                       AGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAG                                                              >  SRR19973065.548003/1‑150 (MQ=60)
                                                                                       AGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAG                                                              <  SRR19973065.1176142/150‑1 (MQ=60)
                                                                                       AGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAG                                                              >  SRR19973065.1423785/1‑150 (MQ=60)
                                                                                          CCTCACCCACTTGCGCATTTACGTCGCAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTT                                                           <  SRR19973065.1669453/150‑1 (MQ=60)
                                                                                          CCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTT                                                           <  SRR19973065.3050547/150‑1 (MQ=60)
                                                                                          CCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTT                                                           >  SRR19973065.2317444/1‑150 (MQ=60)
                                                                                           CTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTG                                                          <  SRR19973065.2372572/150‑1 (MQ=60)
                                                                                               CCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGC                                                      <  SRR19973065.926095/150‑1 (MQ=60)
                                                                                               CCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGC                                                      <  SRR19973065.1132719/150‑1 (MQ=60)
                                                                                               CCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGC                                                      <  SRR19973065.1132766/150‑1 (MQ=60)
                                                                                               CCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGC                                                      <  SRR19973065.1132887/150‑1 (MQ=60)
                                                                                                CCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCG                                                     >  SRR19973065.3079648/1‑150 (MQ=60)
                                                                                                CCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCG                                                     <  SRR19973065.2620427/150‑1 (MQ=60)
                                                                                                CCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCG                                                     >  SRR19973065.3079589/1‑150 (MQ=60)
                                                                                                 CACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGC                                                    >  SRR19973065.513550/1‑150 (MQ=60)
                                                                                                 CACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGC                                                    >  SRR19973065.515151/1‑150 (MQ=60)
                                                                                                  ACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCG                                                   >  SRR19973065.625882/1‑150 (MQ=60)
                                                                                                   CTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGA                                                  >  SRR19973065.301278/1‑150 (MQ=60)
                                                                                                      GCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGG                                               <  SRR19973065.123040/150‑1 (MQ=60)
                                                                                                       CGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGG                                              >  SRR19973065.3354075/1‑150 (MQ=60)
                                                                                                         CATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGAT                                            >  SRR19973065.2680086/1‑150 (MQ=60)
                                                                                                          ATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATG                                           >  SRR19973065.1343604/1‑150 (MQ=60)
                                                                                                            TTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAA                                         <  SRR19973065.1862673/150‑1 (MQ=60)
                                                                                                              ACGTCGAAGGGTCTATTGATTTCCCCGATGAAGAGATATATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGAAGTTATTGCCGATCTTGATGCAGTGCGTTCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGATAG                                       <  SRR19973065.2856842/150‑1 (MQ=60)
                                                                                                              ACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAG                                       <  SRR19973065.2565181/150‑1 (MQ=60)
                                                                                                                GTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTG                                     >  SRR19973065.585904/1‑150 (MQ=60)
                                                                                                                GTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTG                                     >  SRR19973065.1750635/1‑150 (MQ=60)
                                                                                                                GTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTG                                     >  SRR19973065.1750421/1‑150 (MQ=60)
                                                                                                                  CGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGT                                   >  SRR19973065.3097216/1‑150 (MQ=60)
                                                                                                                  CGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGT                                   >  SRR19973065.1339983/1‑150 (MQ=60)
                                                                                                                  CGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGT                                   >  SRR19973065.1473838/1‑150 (MQ=60)
                                                                                                                      GCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATT                               <  SRR19973065.2736828/150‑1 (MQ=60)
                                                                                                                        GGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGC                             >  SRR19973065.1030035/1‑150 (MQ=60)
                                                                                                                        GGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGC                             >  SRR19973065.1030006/1‑150 (MQ=60)
                                                                                                                        GGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGC                             >  SRR19973065.1029997/1‑150 (MQ=60)
                                                                                                                           AATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGG                          <  SRR19973065.3330018/150‑1 (MQ=60)
                                                                                                                             TTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGAC                        <  SRR19973065.2482479/150‑1 (MQ=60)
                                                                                                                               GATTTCGCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGT                      <  SRR19973065.2375810/150‑1 (MQ=60)
                                                                                                                               GATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGT                      <  SRR19973065.2375729/150‑1 (MQ=60)
                                                                                                                                ATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTC                     >  SRR19973065.807712/1‑150 (MQ=60)
                                                                                                                                   TCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTA                  <  SRR19973065.2918023/150‑1 (MQ=60)
                                                                                                                                     CCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAAC                >  SRR19973065.2704116/1‑150 (MQ=60)
                                                                                                                                      CCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACG               >  SRR19973065.2364254/1‑150 (MQ=60)
                                                                                                                                        GATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCC             >  SRR19973065.3266678/1‑150 (MQ=60)
                                                                                                                                         ATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCG            <  SRR19973065.2117314/150‑1 (MQ=60)
                                                                                                                                          TGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGG           >  SRR19973065.151944/1‑150 (MQ=60)
                                                                                                                                          TGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGG           >  SRR19973065.152864/1‑150 (MQ=60)
                                                                                                                                           GAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATATTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGT          <  SRR19973065.2367176/150‑1 (MQ=60)
                                                                                                                                            AAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTA         >  SRR19973065.1143099/1‑150 (MQ=60)
                                                                                                                                            AAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTA         >  SRR19973065.1143118/1‑150 (MQ=60)
                                                                                                                                                   CTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAATCGAG  >  SRR19973065.2222911/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
TATCGACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCGATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAATCGAG  >  CP009273/3880583‑3880879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑