Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,872,006 T→A 79.4% S492C (AGC→TGC)  gyrB ← DNA gyrase, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,872,0060TA79.4% 608.9 / 151.4 257S492C (AGC→TGC) gyrBDNA gyrase, subunit B
Reads supporting (aligned to +/- strand):  ref base T (26/27);  new base A (115/89);  total (141/116)
Fisher's exact test for biased strand distribution p-value = 3.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 262 total aligned reads displayed.

CCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAA  >  CP009273/3871861‑3872152
                                                                                                                                                 |                                                                                                                                                  
ccTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCagtg                                                                                                                                                 >  2:1517701/1‑149 (MQ=255)
ccTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCagtg                                                                                                                                                 <  2:3965597/149‑1 (MQ=255)
 cTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGa                                                                                                                                                >  1:3729195/1‑149 (MQ=255)
  tttCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGAt                                                                                                                                               >  2:3340623/1‑149 (MQ=255)
        cACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCa                                                                                                                                         >  1:2269780/1‑149 (MQ=255)
            ttGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGttt                                                                                                                                     <  2:541675/149‑1 (MQ=255)
                aCAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTcc                                                                                                                                 >  1:3933052/1‑149 (MQ=255)
                 cAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCg                                                                                                                                <  1:4461908/149‑1 (MQ=255)
                   gcggcgGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCggg                                                                                                                              >  2:1187568/1‑149 (MQ=255)
                    cggcggCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGt                                                                                                                             <  1:1680974/149‑1 (MQ=255)
                    cggcggCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGt                                                                                                                             <  1:4645463/149‑1 (MQ=255)
                         gcTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTAc                                                                                                                        <  1:4025013/149‑1 (MQ=255)
                           tGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTc                                                                                                                      <  1:868348/149‑1 (MQ=255)
                           tGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTc                                                                                                                      >  1:1028858/1‑149 (MQ=255)
                           tGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTc                                                                                                                      <  1:596256/149‑1 (MQ=255)
                           tGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTc                                                                                                                      >  2:433545/1‑149 (MQ=255)
                            gAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCg                                                                                                                     >  2:70384/1‑149 (MQ=255)
                             aGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGt                                                                                                                    <  2:2320350/149‑1 (MQ=255)
                               cGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCa                                                                                                                  <  2:2587568/149‑1 (MQ=255)
                                gATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCAc                                                                                                                 >  2:2398482/1‑149 (MQ=255)
                                gATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCAc                                                                                                                 >  1:2361831/1‑149 (MQ=255)
                                  tGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGa                                                                                                               <  2:2549379/149‑1 (MQ=255)
                                  tGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGa                                                                                                               >  2:1901212/1‑149 (MQ=255)
                                    tAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGAcc                                                                                                             <  1:2758830/149‑1 (MQ=255)
                                     aGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACGGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCg                                                                                                            >  2:121508/1‑149 (MQ=255)
                                      gACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGa                                                                                                           >  2:2106829/1‑149 (MQ=255)
                                      gACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGa                                                                                                           >  1:4076157/1‑149 (MQ=255)
                                          tGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATAcc                                                                                                       >  1:4412817/1‑149 (MQ=255)
                                                  ttCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCATACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCaa                                                                                               <  2:709250/149‑1 (MQ=255)
                                                     aaCGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAgcg                                                                                            >  1:4656890/1‑149 (MQ=255)
                                                     aaCGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAgcg                                                                                            >  1:3447650/1‑149 (MQ=255)
                                                       cGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCCTGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGcgcg                                                                                          >  1:3002653/1‑149 (MQ=255)
                                                         aTTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGt                                                                                        <  1:10894/149‑1 (MQ=255)
                                                               ggCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAg                                                                                  >  2:1283805/1‑149 (MQ=255)
                                                               ggCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAg                                                                                  <  1:4772950/149‑1 (MQ=255)
                                                                   tCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTc                                                                              >  1:1859403/1‑149 (MQ=255)
                                                                   tCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTC‑‑‑ATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGcc                                                                           >  1:3604004/1‑149 (MQ=255)
                                                                      gACGATAGAAGAAGGTCAACAGCAGCGTAAGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACAGATACCACAGCCAAGCGCGGTGATAAGCGTCGcc                                                                           >  1:3301041/1‑149 (MQ=255)
                                                                        cGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCAc                                                                         >  2:43918/1‑149 (MQ=255)
                                                                         gATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACt                                                                        <  2:877809/149‑1 (MQ=255)
                                                                          aTAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACtt                                                                       >  1:2177189/1‑149 (MQ=255)
                                                                          aTAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACtt                                                                       >  1:4575099/1‑149 (MQ=255)
                                                                            agaagaAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTcc                                                                     <  1:2526187/149‑1 (MQ=255)
                                                                            agaagaAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTcc                                                                     <  2:4628146/149‑1 (MQ=255)
                                                                             gaagaaGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCt                                                                    <  1:2089855/149‑1 (MQ=255)
                                                                              aagaagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTg                                                                   <  1:1307532/149‑1 (MQ=255)
                                                                                gaagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTgag                                                                 <  1:2426101/149‑1 (MQ=255)
                                                                                gaagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTgag                                                                 <  1:32870/149‑1 (MQ=255)
                                                                                 aagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTgaga                                                                <  2:2013813/149‑1 (MQ=255)
                                                                                  agGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaa                                                               >  1:3188952/1‑149 (MQ=255)
                                                                                   ggTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaag                                                              <  1:256305/149‑1 (MQ=255)
                                                                                    gTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaaga                                                             >  2:2091079/1‑149 (MQ=255)
                                                                                    gTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaaga                                                             <  2:1687508/149‑1 (MQ=255)
                                                                                       aaCAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagagag                                                          >  2:27429/1‑149 (MQ=255)
                                                                                        aCAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGc                                                         <  2:420567/149‑1 (MQ=255)
                                                                                        aCAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGc                                                         <  2:3655888/149‑1 (MQ=255)
                                                                                          agcagcGGACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATACCGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCAt                                                       <  2:4546028/149‑1 (MQ=255)
                                                                                           gcagcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATc                                                      <  2:4254933/149‑1 (MQ=255)
                                                                                            cagcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCt                                                     <  2:3203953/149‑1 (MQ=255)
                                                                                            cagcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCt                                                     >  1:399837/1‑149 (MQ=255)
                                                                                             agcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCtt                                                    >  1:1001906/1‑149 (MQ=255)
                                                                                              gcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTa                                                   >  2:3672077/1‑149 (MQ=255)
                                                                                               cGTACGACTGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGACGAGAGCATCGTAt                                                  <  1:3872239/149‑1 (MQ=255)
                                                                                               cGTACGAATGTGCGAGCAGTCGACGTCCGCATCGTTCATGATGATGTTGCAGTGATAACGCTGTTTGTCCGGGTTGTACTAGTCACGACCGATACCACAGCCAAGCGCGGTTATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTAt                                                  <  1:3195367/149‑1 (MQ=255)
                                                                                                gTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATc                                                 <  2:2079606/149‑1 (MQ=255)
                                                                                                gTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATc                                                 >  2:1608032/1‑149 (MQ=255)
                                                                                                gTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATc                                                 <  2:2781336/149‑1 (MQ=255)
                                                                                                    gAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAg                                             <  1:1149862/149‑1 (MQ=255)
                                                                                                    gAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAg                                             >  1:3603101/1‑149 (MQ=255)
                                                                                                      aTGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAgcg                                           >  2:928057/1‑149 (MQ=255)
                                                                                                         tgCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAgcgcgc                                        <  2:961522/149‑1 (MQ=255)
                                                                                                              gCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTtct                                   <  1:3082117/149‑1 (MQ=255)
                                                                                                              gCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTtct                                   <  1:690418/149‑1 (MQ=255)
                                                                                                               ccGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTtctc                                  >  1:2579751/1‑149 (MQ=255)
                                                                                                                cGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCg                                 <  1:4093846/149‑1 (MQ=255)
                                                                                                                 gTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGa                                >  1:1842807/1‑149 (MQ=255)
                                                                                                                  tCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGAc                               >  1:1913250/1‑149 (MQ=255)
                                                                                                                  tCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGAc                               >  2:1750851/1‑149 (MQ=255)
                                                                                                                     aCGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAATCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGtt                            >  1:4244391/1‑149 (MQ=255)
                                                                                                                     aCGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGtt                            <  2:396382/149‑1 (MQ=255)
                                                                                                                        tCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAg                         >  2:373323/1‑149 (MQ=255)
                                                                                                                        tCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAg                         <  1:4734869/149‑1 (MQ=255)
                                                                                                                           gCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAt                      <  2:1003144/149‑1 (MQ=255)
                                                                                                                           gCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAt                      <  2:1371572/149‑1 (MQ=255)
                                                                                                                            cATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAtt                     <  2:4644340/149‑1 (MQ=255)
                                                                                                                               cGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTa                  <  1:3063605/149‑1 (MQ=255)
                                                                                                                                   cATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCt              <  1:1544687/149‑1 (MQ=255)
                                                                                                                                    atgatgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCtt             >  2:2384718/1‑149 (MQ=255)
                                                                                                                                      gatgatgatgCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCa           <  2:3512511/149‑1 (MQ=255)
                                                                                                                                       atgatgatgCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAg          <  2:1619413/149‑1 (MQ=255)
                                                                                                                                        tgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACTACCGATACCACAGCCAAGCGCGGTGATAAGCTTCGCCACTTCATGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGc         >  2:4601190/1‑149 (MQ=255)
                                                                                                                                        tgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGc         >  2:4749726/1‑149 (MQ=255)
                                                                                                                                        tgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGc         >  2:2846357/1‑149 (MQ=255)
                                                                                                                                         gatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCg        >  1:912490/1‑149 (MQ=255)
                                                                                                                                          atgatgCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCgg       >  2:1495674/1‑149 (MQ=255)
                                                                                                                                          atgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACGGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCgg       >  2:4549298/1‑149 (MQ=255)
                                                                                                                                          atgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCgg       >  1:3807102/1‑149 (MQ=255)
                                                                                                                                              tgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGa   >  1:2032241/1‑149 (MQ=255)
                                                                                                                                               gCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGaa  <  2:1426820/149‑1 (MQ=255)
                                                                                                                                               gCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGaa  <  2:770476/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAA  >  CP009273/3871861‑3872152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCG  >  CP009273/3871859‑3872155
                                                                                                                                                   |                                                                                                                                                     
TGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGT                                                                                                                                                     <  SRR19973071.2608623/150‑1 (MQ=60)
  CCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGA                                                                                                                                                   <  SRR19973071.1874591/150‑1 (MQ=60)
  CCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGA                                                                                                                                                   >  SRR19973071.3742424/1‑150 (MQ=60)
  CCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGA                                                                                                                                                   >  SRR19973071.2176498/1‑150 (MQ=60)
     TTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAA                                                                                                                                                <  SRR19973071.777944/150‑1 (MQ=60)
     TTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAA                                                                                                                                                <  SRR19973071.4584084/150‑1 (MQ=60)
     TTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAA                                                                                                                                                >  SRR19973071.306039/1‑150 (MQ=60)
       CTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACG                                                                                                                                              <  SRR19973071.1593785/150‑1 (MQ=60)
        TTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGC                                                                                                                                             >  SRR19973071.4777279/1‑150 (MQ=60)
         TCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCA                                                                                                                                            >  SRR19973071.2278128/1‑150 (MQ=60)
          CACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAG                                                                                                                                           >  SRR19973071.1984103/1‑150 (MQ=60)
          CACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAG                                                                                                                                           <  SRR19973071.32128/150‑1 (MQ=60)
          CACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAG                                                                                                                                           <  SRR19973071.3954177/150‑1 (MQ=60)
          CACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAG                                                                                                                                           >  SRR19973071.4167333/1‑150 (MQ=60)
          CACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAG                                                                                                                                           <  SRR19973071.3954278/150‑1 (MQ=60)
           ACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGT                                                                                                                                          >  SRR19973071.3104063/1‑150 (MQ=60)
               TGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGT                                                                                                                                      <  SRR19973071.2098366/150‑1 (MQ=60)
                GTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTC                                                                                                                                     <  SRR19973071.304673/150‑1 (MQ=60)
                 TACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCC                                                                                                                                    >  SRR19973071.1404180/1‑150 (MQ=60)
                 TACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCC                                                                                                                                    >  SRR19973071.3946896/1‑150 (MQ=60)
                  ACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCG                                                                                                                                   >  SRR19973071.2981623/1‑150 (MQ=60)
                   CAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGG                                                                                                                                  <  SRR19973071.4477714/150‑1 (MQ=60)
                   CAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGG                                                                                                                                  >  SRR19973071.2044641/1‑150 (MQ=60)
                    AGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATTGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGG                                                                                                                                 <  SRR19973071.3569738/150‑1 (MQ=60)
                      CGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTT                                                                                                                               <  SRR19973071.4662057/150‑1 (MQ=60)
                      CGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTT                                                                                                                               <  SRR19973071.4662010/150‑1 (MQ=60)
                      CGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTT                                                                                                                               <  SRR19973071.1687166/150‑1 (MQ=60)
                        GCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGT                                                                                                                             <  SRR19973071.2424319/150‑1 (MQ=60)
                         CGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTA                                                                                                                            >  SRR19973071.3255308/1‑150 (MQ=60)
                          GGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTAC                                                                                                                           >  SRR19973071.650221/1‑150 (MQ=60)
                           GCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACT                                                                                                                          <  SRR19973071.4039098/150‑1 (MQ=60)
                           GCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACT                                                                                                                          <  SRR19973071.4039179/150‑1 (MQ=60)
                            CTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTC                                                                                                                         >  SRR19973071.1032574/1‑150 (MQ=60)
                            CTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTC                                                                                                                         >  SRR19973071.4346643/1‑150 (MQ=60)
                             TGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCG                                                                                                                        <  SRR19973071.871454/150‑1 (MQ=60)
                             TGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCG                                                                                                                        <  SRR19973071.598475/150‑1 (MQ=60)
                                 CGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCAC                                                                                                                    <  SRR19973071.4063752/150‑1 (MQ=60)
                                 CGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCAC                                                                                                                    >  SRR19973071.2370380/1‑150 (MQ=60)
                                      TAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCG                                                                                                               <  SRR19973071.2768576/150‑1 (MQ=60)
                                       AGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGA                                                                                                              >  SRR19973071.4090612/1‑150 (MQ=60)
                                          CGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATAC                                                                                                           >  SRR19973071.4266348/1‑150 (MQ=60)
                                          CGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATAC                                                                                                           >  SRR19973071.4067933/1‑150 (MQ=60)
                                           GTGACCGTGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACC                                                                                                          >  SRR19973071.4428459/1‑150 (MQ=60)
                                           GTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACC                                                                                                          >  SRR19973071.4428350/1‑150 (MQ=60)
                                                  CGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCA                                                                                                   >  SRR19973071.2617725/1‑150 (MQ=60)
                                                    TTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAG                                                                                                 <  SRR19973071.3618027/150‑1 (MQ=60)
                                                      CAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCG                                                                                               >  SRR19973071.3459820/1‑150 (MQ=60)
                                                      CAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCG                                                                                               >  SRR19973071.4673475/1‑150 (MQ=60)
                                                        ACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCCTGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCG                                                                                             >  SRR19973071.3013382/1‑150 (MQ=60)
                                                        ACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCG                                                                                             >  SRR19973071.3930813/1‑150 (MQ=60)
                                                         CGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGG                                                                                            <  SRR19973071.364356/150‑1 (MQ=60)
                                                           ATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTG                                                                                          >  SRR19973071.985258/1‑150 (MQ=60)
                                                           ATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTG                                                                                          <  SRR19973071.10970/150‑1 (MQ=60)
                                                           ATTCCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTG                                                                                          <  SRR19973071.10976/150‑1 (MQ=60)
                                                              TCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATA                                                                                       >  SRR19973071.61723/1‑150 (MQ=60)
                                                               CCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAA                                                                                      <  SRR19973071.1428266/150‑1 (MQ=60)
                                                                CGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAG                                                                                     <  SRR19973071.4558194/150‑1 (MQ=60)
                                                                CGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAG                                                                                     <  SRR19973071.3389919/150‑1 (MQ=60)
                                                                 GGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGC                                                                                    <  SRR19973071.4789798/150‑1 (MQ=60)
                                                                    ATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTC                                                                                 <  SRR19973071.2547287/150‑1 (MQ=60)
                                                                    ATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTC                                                                                 >  SRR19973071.1866278/1‑150 (MQ=60)
                                                                    ATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTC                                                                                 <  SRR19973071.1215572/150‑1 (MQ=60)
                                                                    ATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTC‑‑‑ATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCC                                                                              >  SRR19973071.3616547/1‑150 (MQ=60)
                                                                    ATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTC‑‑‑ATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCC                                                                              >  SRR19973071.3616576/1‑150 (MQ=60)
                                                                      CTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGC                                                                               >  SRR19973071.1712775/1‑150 (MQ=60)
                                                                       TGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCC                                                                              >  SRR19973071.1714231/1‑150 (MQ=60)
                                                                       TGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCC                                                                              >  SRR19973071.3312681/1‑150 (MQ=60)
                                                                       TGACGATAGAAGAAGGTCAACAGCAGCGTAAGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACAGATACCACAGCCAAGCGCGGTGATAAGCGTCGCC                                                                              >  SRR19973071.3312740/1‑150 (MQ=60)
                                                                           GATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTT                                                                          >  SRR19973071.3165649/1‑150 (MQ=60)
                                                                           GATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTT                                                                          >  SRR19973071.4591405/1‑150 (MQ=60)
                                                                           GATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTT                                                                          >  SRR19973071.2185240/1‑150 (MQ=60)
                                                                              AGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCT                                                                       <  SRR19973071.2535377/150‑1 (MQ=60)
                                                                               GAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTG                                                                      <  SRR19973071.2097555/150‑1 (MQ=60)
                                                                                AAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGA                                                                     <  SRR19973071.1312313/150‑1 (MQ=60)
                                                                                 AGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAG                                                                    <  SRR19973071.2482786/150‑1 (MQ=60)
                                                                                  GAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGA                                                                   <  SRR19973071.33097/150‑1 (MQ=60)
                                                                                  GAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGA                                                                   <  SRR19973071.33084/150‑1 (MQ=60)
                                                                                  GAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGA                                                                   <  SRR19973071.2434875/150‑1 (MQ=60)
                                                                                   AAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAA                                                                  >  SRR19973071.3200046/1‑150 (MQ=60)
                                                                                    AGGTCAACTGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAG                                                                 <  SRR19973071.122094/150‑1 (MQ=60)
                                                                                     GGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGA                                                                <  SRR19973071.257283/150‑1 (MQ=60)
                                                                                     GGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGA                                                                >  SRR19973071.2778100/1‑150 (MQ=60)
                                                                                     GGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGA                                                                <  SRR19973071.257270/150‑1 (MQ=60)
                                                                                     GGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGA                                                                >  SRR19973071.378206/1‑150 (MQ=60)
                                                                                          ACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCA                                                           <  SRR19973071.3519530/150‑1 (MQ=60)
                                                                                           CAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCAT                                                          >  SRR19973071.4600783/1‑150 (MQ=60)
                                                                                           CAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCAT                                                          >  SRR19973071.1032696/1‑150 (MQ=60)
                                                                                           CAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCAT                                                          >  SRR19973071.1032680/1‑150 (MQ=60)
                                                                                            AGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATC                                                         >  SRR19973071.2219909/1‑150 (MQ=60)
                                                                                            AGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATC                                                         >  SRR19973071.567814/1‑150 (MQ=60)
                                                                                             GCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCT                                                        <  SRR19973071.4712/150‑1 (MQ=60)
                                                                                             GCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCT                                                        >  SRR19973071.401076/1‑150 (MQ=60)
                                                                                              CAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTT                                                       >  SRR19973071.1005549/1‑150 (MQ=60)
                                                                                               AGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTA                                                      <  SRR19973071.4628882/150‑1 (MQ=60)
                                                                                               AGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTA                                                      >  SRR19973071.2322785/1‑150 (MQ=60)
                                                                                                 CGTACGACTGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGACGAGAGCATCGTATC                                                    <  SRR19973071.3885938/150‑1 (MQ=60)
                                                                                                 CGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATC                                                    <  SRR19973071.1006538/150‑1 (MQ=60)
                                                                                                 CGTACGAATGTGCGAGCAGTCGACGTCCGCATCGTTCATGATGATGTTGCAGTGATAACGCTGTTTGTCCGGGTTGTACTAGTCACGACCGATACCACAGCCAAGCGCGGTTATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATC                                                    <  SRR19973071.3206481/150‑1 (MQ=60)
                                                                                                  GTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCG                                                   >  SRR19973071.4102859/1‑150 (MQ=60)
                                                                                                  GTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCG                                                   >  SRR19973071.378898/1‑150 (MQ=60)
                                                                                                     CGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAG                                                >  SRR19973071.3615667/1‑150 (MQ=60)
                                                                                                      GAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGC                                               <  SRR19973071.1153865/150‑1 (MQ=60)
                                                                                                       AATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCG                                              >  SRR19973071.3260869/1‑150 (MQ=60)
                                                                                                                GCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTC                                     <  SRR19973071.95344/150‑1 (MQ=60)
                                                                                                                GCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTC                                     <  SRR19973071.692867/150‑1 (MQ=60)
                                                                                                                GCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTC                                     <  SRR19973071.3093021/150‑1 (MQ=60)
                                                                                                                GCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTC                                     >  SRR19973071.2589105/1‑150 (MQ=60)
                                                                                                                GCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTC                                     >  SRR19973071.2589098/1‑150 (MQ=60)
                                                                                                                  CGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGA                                   >  SRR19973071.1849826/1‑150 (MQ=60)
                                                                                                                  CGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGA                                   <  SRR19973071.4108392/150‑1 (MQ=60)
                                                                                                                  CGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGA                                   >  SRR19973071.1849639/1‑150 (MQ=60)
                                                                                                                  CGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGA                                   <  SRR19973071.3875043/150‑1 (MQ=60)
                                                                                                                   GTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGAC                                  >  SRR19973071.1920257/1‑150 (MQ=60)
                                                                                                                   GTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGAC                                  >  SRR19973071.1920070/1‑150 (MQ=60)
                                                                                                                      GACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAATCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTT                               >  SRR19973071.4259466/1‑150 (MQ=60)
                                                                                                                      GACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTT                               <  SRR19973071.1977924/150‑1 (MQ=60)
                                                                                                                      GACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTT                               >  SRR19973071.4259432/1‑150 (MQ=60)
                                                                                                                      GACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTT                               >  SRR19973071.523444/1‑150 (MQ=60)
                                                                                                                          TCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGG                           <  SRR19973071.4751628/150‑1 (MQ=60)
                                                                                                                             GCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATT                        >  SRR19973071.2237547/1‑150 (MQ=60)
                                                                                                                                 CGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAC                    <  SRR19973071.3074473/150‑1 (MQ=60)
                                                                                                                                 CGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAC                    <  SRR19973071.3074468/150‑1 (MQ=60)
                                                                                                                                     CATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTT                <  SRR19973071.1550215/150‑1 (MQ=60)
                                                                                                                                       TGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCA              <  SRR19973071.3911536/150‑1 (MQ=60)
                                                                                                                                        GATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAG             <  SRR19973071.2571878/150‑1 (MQ=60)
                                                                                                                                          TGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCG           >  SRR19973071.915331/1‑150 (MQ=60)
                                                                                                                                          TGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCG           >  SRR19973071.915786/1‑150 (MQ=60)
                                                                                                                                          TGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCG           >  SRR19973071.915790/1‑150 (MQ=60)
                                                                                                                                           GATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGG          >  SRR19973071.3820617/1‑150 (MQ=60)
                                                                                                                                            ATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGC         >  SRR19973071.2423756/1‑150 (MQ=60)
                                                                                                                                              GATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAG       >  SRR19973071.4707268/1‑150 (MQ=60)
                                                                                                                                               ATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGA      >  SRR19973071.2039663/1‑150 (MQ=60)
                                                                                                                                                  CAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATC   <  SRR19973071.3743011/150‑1 (MQ=60)
                                                                                                                                                  CAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATC   <  SRR19973071.3742861/150‑1 (MQ=60)
                                                                                                                                                   AGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCG  >  SRR19973071.2103756/1‑150 (MQ=60)
                                                                                                                                                   AGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCG  >  SRR19973071.459904/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
TGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCG  >  CP009273/3871859‑3872155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑