Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,872,006 T→A 100% S492C (AGC→TGC)  gyrB ← DNA gyrase, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,872,0060TA98.9% 600.3 / ‑2.4 181S492C (AGC→TGC) gyrBDNA gyrase, subunit B
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base A (70/109);  total (70/111)
Fisher's exact test for biased strand distribution p-value = 5.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 181 total aligned reads displayed.

TTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAG  >  CP009273/3871863‑3872150
                                                                                                                                               |                                                                                                                                                
tttCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGa                                                                                                                                              <  2:2468197/148‑1 (MQ=255)
tttCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGa                                                                                                                                              <  1:3482999/148‑1 (MQ=255)
 ttcttcACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGAt                                                                                                                                             <  1:979825/148‑1 (MQ=255)
 ttcttcACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGAt                                                                                                                                             <  1:3276229/148‑1 (MQ=255)
 ttcttcACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGATCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGAt                                                                                                                                             >  1:1246708/1‑148 (MQ=255)
 ttcttcACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGATCAACGATTTCCGGCATCAGACGATAGAAGAAGGTCAACAGAAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCAGGAGGATGATGCAGTGAt                                                                                                                                             >  1:3107433/1‑148 (MQ=255)
  tcttcACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATa                                                                                                                                            <  2:252428/148‑1 (MQ=255)
  tcttcACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATa                                                                                                                                            >  1:3245495/1‑148 (MQ=255)
   cttcACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATaa                                                                                                                                           <  2:1388646/148‑1 (MQ=255)
   cttcACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATaa                                                                                                                                           <  1:1729770/148‑1 (MQ=255)
       aCTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCa                                                                                                                                       <  2:871137/148‑1 (MQ=255)
        cTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAg                                                                                                                                      <  2:3294877/148‑1 (MQ=255)
         tttGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGt                                                                                                                                     >  2:3029378/1‑148 (MQ=255)
               cAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTcc                                                                                                                               >  1:1451369/1‑148 (MQ=255)
               cAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTcc                                                                                                                               >  1:1959103/1‑148 (MQ=255)
                     cggcTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTg                                                                                                                         <  1:1031399/148‑1 (MQ=255)
                     cggcTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTg                                                                                                                         <  1:3701153/148‑1 (MQ=255)
                       gcTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTa                                                                                                                       <  2:1942735/148‑1 (MQ=255)
                       gcTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTa                                                                                                                       <  1:998334/148‑1 (MQ=255)
                        cTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTAc                                                                                                                      >  2:3321410/1‑148 (MQ=255)
                         tGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACt                                                                                                                     <  1:3315354/148‑1 (MQ=255)
                           aGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCg                                                                                                                   >  1:2398620/1‑148 (MQ=255)
                            gCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGt                                                                                                                  <  1:3473096/148‑1 (MQ=255)
                             cGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTc                                                                                                                 <  2:2288107/148‑1 (MQ=255)
                             cGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTc                                                                                                                 <  2:2284200/148‑1 (MQ=255)
                              gATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCa                                                                                                                <  2:2995215/148‑1 (MQ=255)
                                     aCGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGa                                                                                                         <  2:3017514/148‑1 (MQ=255)
                                          aCCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACca                                                                                                    <  2:2467558/148‑1 (MQ=255)
                                                 tCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCaa                                                                                             <  2:2716631/148‑1 (MQ=255)
                                                 tCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCaa                                                                                             >  2:2178296/1‑148 (MQ=255)
                                                  cAACGATTTCCGTCATCTGACGATAGAAGATGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAg                                                                                            <  1:542776/148‑1 (MQ=255)
                                                  cAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAg                                                                                            <  2:401115/148‑1 (MQ=255)
                                                    aCGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAgcg                                                                                          >  2:3075614/1‑148 (MQ=255)
                                                     cGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAgcgc                                                                                         >  1:2450993/1‑148 (MQ=255)
                                                      gATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTAGTACTCGTCACGACCGATACCACAGCCAAGcgcg                                                                                        <  2:2440817/148‑1 (MQ=255)
                                                        tttCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGt                                                                                      <  1:1225443/148‑1 (MQ=255)
                                                          tCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCTGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGa                                                                                    <  2:2733575/148‑1 (MQ=255)
                                                           ccGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGAt                                                                                   <  2:476521/148‑1 (MQ=255)
                                                              gCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACTAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAg                                                                                <  2:813852/148‑1 (MQ=255)
                                                                aTCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCg                                                                              <  1:1081997/148‑1 (MQ=255)
                                                                 tCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGt                                                                             <  1:1084283/148‑1 (MQ=255)
                                                                        aTAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACt                                                                      >  1:2689915/1‑148 (MQ=255)
                                                                        aTAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACt                                                                      >  1:3764102/1‑148 (MQ=255)
                                                                         tAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTTTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACtt                                                                     <  2:620005/148‑1 (MQ=255)
                                                                         tAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACtt                                                                     <  1:3444919/148‑1 (MQ=255)
                                                                         tAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACtt                                                                     >  2:1526371/1‑148 (MQ=255)
                                                                          agaagaAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTc                                                                    <  2:1720320/148‑1 (MQ=255)
                                                                          agaagaAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTc                                                                    <  2:2343004/148‑1 (MQ=255)
                                                                              gaagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTga                                                                >  1:1439903/1‑148 (MQ=255)
                                                                                agGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTgaga                                                              <  1:1145849/148‑1 (MQ=255)
                                                                                agGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTgaga                                                              <  2:1041956/148‑1 (MQ=255)
                                                                                   tCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaaga                                                           <  2:2894457/148‑1 (MQ=255)
                                                                                    cAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagag                                                          <  2:536885/148‑1 (MQ=255)
                                                                                    cAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagag                                                          <  2:2902384/148‑1 (MQ=255)
                                                                                     aaCAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagaga                                                         <  2:1826490/148‑1 (MQ=255)
                                                                                      aCAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagagag                                                        >  1:1323232/1‑148 (MQ=255)
                                                                                       cagcagCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGc                                                       <  1:2446280/148‑1 (MQ=255)
                                                                                       cagcagCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGc                                                       >  1:1645018/1‑148 (MQ=255)
                                                                                        agcagcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCa                                                      >  2:558286/1‑148 (MQ=255)
                                                                                        agcagcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGAGAAGCGTCGCCACTTCCTGAGAAGAGAGCa                                                      >  2:558289/1‑148 (MQ=255)
                                                                                         gcagcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCAt                                                     <  1:1784899/148‑1 (MQ=255)
                                                                                         gcagcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCAt                                                     <  1:3444082/148‑1 (MQ=255)
                                                                                          cagcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATc                                                    <  1:1466033/148‑1 (MQ=255)
                                                                                          cagcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATc                                                    <  2:3739210/148‑1 (MQ=255)
                                                                                             cGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTa                                                 >  1:2231407/1‑148 (MQ=255)
                                                                                             cGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTa                                                 >  2:3740448/1‑148 (MQ=255)
                                                                                              gTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTAt                                                <  2:2023395/148‑1 (MQ=255)
                                                                                                 cGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGa                                             <  2:200421/148‑1 (MQ=255)
                                                                                                  tattgtgCGAGACGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGTTACGACTGCCAAGCGCGGTGATAAGCGTCGCCACTTGCTGAGAAGTGAGCATCTTATCTaa                                            <  2:3482208/145‑1 (MQ=255)
                                                                                                  gAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGTTAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGaa                                            <  2:353981/148‑1 (MQ=255)
                                                                                                    aTGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAgc                                          >  1:1134482/1‑148 (MQ=255)
                                                                                                        gCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAgcgcgc                                      <  2:3194077/148‑1 (MQ=255)
                                                                                                          gAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCtt                                    <  2:1695040/148‑1 (MQ=255)
                                                                                                             ccGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTtct                                 >  1:632662/1‑148 (MQ=255)
                                                                                                             ccGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTtct                                 >  1:2549611/1‑148 (MQ=255)
                                                                                                             ccGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTtct                                 >  1:2763758/1‑148 (MQ=255)
                                                                                                                  gaggTCCGCATCGGTCATGTTTATTATGCAGTTTTAACGCAGTTTGTCCGGGTTGTACTGGTCACGACCGATACCACAGCCAAGCGCGGTGATTAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGAct                            <  1:150501/145‑2 (MQ=255)
                                                                                                                  gACGTCCACATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACg                            <  2:3275719/148‑1 (MQ=255)
                                                                                                                    cGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGtt                          >  1:3185064/1‑148 (MQ=255)
                                                                                                                      tCCGCATCGGTCATGCTGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGa                        <  2:2821755/148‑1 (MQ=255)
                                                                                                                          cATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAt                    <  1:1844658/148‑1 (MQ=255)
                                                                                                                           aTCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAtt                   <  1:2275694/148‑1 (MQ=255)
                                                                                                                            tCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAttt                  <  2:613563/148‑1 (MQ=255)
                                                                                                                             cGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAtttt                 <  1:307737/148‑1 (MQ=255)
                                                                                                                              ggTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTa                <  2:1850538/148‑1 (MQ=255)
                                                                                                                              ggTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTa                <  2:146770/148‑1 (MQ=255)
                                                                                                                               gTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAc               <  1:848421/148‑1 (MQ=255)
                                                                                                                                 cATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAccc             <  2:3107431/148‑1 (MQ=255)
                                                                                                                                 cATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAccc             >  1:2955225/1‑148 (MQ=255)
                                                                                                                                 cATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAccc             >  1:2955482/1‑148 (MQ=255)
                                                                                                                                 cATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAccc             >  2:2918620/1‑148 (MQ=255)
                                                                                                                                 cATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAccc             >  1:130881/1‑148 (MQ=255)
                                                                                                                                   tgatgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCtt           >  2:1157709/1‑148 (MQ=255)
                                                                                                                                    gatgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCttt                                   <  2:2520330/123‑1 (MQ=255)
                                                                                                                                    gatgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTc          >  1:3439024/1‑148 (MQ=255)
                                                                                                                                    gatgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTc          >  2:208210/1‑148 (MQ=255)
                                                                                                                                     atgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCa         >  2:1939137/1‑148 (MQ=255)
                                                                                                                                     atgatgatgCAGTGATAACGCAGTTTGTCCGGGTGGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCa         >  1:3558012/1‑148 (MQ=255)
                                                                                                                                        atgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCg      >  1:3293616/1‑148 (MQ=255)
                                                                                                                                            tgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAg  >  2:2429112/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                
TTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAG  >  CP009273/3871863‑3872150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCG  >  CP009273/3871859‑3872155
                                                                                                                                                   |                                                                                                                                                     
TGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGT                                                                                                                                                     <  SRR19973069.3252962/150‑1 (MQ=60)
   CTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGAT                                                                                                                                                  >  SRR19973069.148081/1‑150 (MQ=60)
   CTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGAT                                                                                                                                                  >  SRR19973069.3127873/1‑150 (MQ=60)
   CTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGAT                                                                                                                                                  >  SRR19973069.1863461/1‑150 (MQ=60)
   CTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGATCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGAT                                                                                                                                                  >  SRR19973069.1255166/1‑150 (MQ=60)
   CTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGATCAACGATTTCCGGCATCAGACGATAGAAGAAGGTCAACAGAAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCAGGAGGATGATGCAGTGAT                                                                                                                                                  >  SRR19973069.3127875/1‑150 (MQ=60)
    TTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATA                                                                                                                                                 <  SRR19973069.3505487/150‑1 (MQ=60)
    TTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATA                                                                                                                                                 >  SRR19973069.3266738/1‑150 (MQ=60)
     TTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAA                                                                                                                                                <  SRR19973069.986429/150‑1 (MQ=60)
     TTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAA                                                                                                                                                <  SRR19973069.3297681/150‑1 (MQ=60)
       CTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACG                                                                                                                                              <  SRR19973069.1400388/150‑1 (MQ=60)
       CTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACG                                                                                                                                              <  SRR19973069.1741900/150‑1 (MQ=60)
       CTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACG                                                                                                                                              >  SRR19973069.2900871/1‑150 (MQ=60)
          CACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAG                                                                                                                                           <  SRR19973069.2125852/150‑1 (MQ=60)
          CACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAG                                                                                                                                           >  SRR19973069.2624308/1‑150 (MQ=60)
                 TACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCC                                                                                                                                    >  SRR19973069.1972679/1‑150 (MQ=60)
                 TACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCC                                                                                                                                    >  SRR19973069.1461501/1‑150 (MQ=60)
                         CGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTA                                                                                                                            <  SRR19973069.1038541/150‑1 (MQ=60)
                         CGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTA                                                                                                                            <  SRR19973069.3725342/150‑1 (MQ=60)
                           GCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACT                                                                                                                          <  SRR19973069.1005165/150‑1 (MQ=60)
                           GCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACT                                                                                                                          <  SRR19973069.1004943/150‑1 (MQ=60)
                             TGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCG                                                                                                                        >  SRR19973069.2414865/1‑150 (MQ=60)
                             TGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCG                                                                                                                        <  SRR19973069.3337093/150‑1 (MQ=60)
                                GCTATGTAGACGTTACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCA                                                                                                                     <  SRR19973069.1679174/150‑1 (MQ=60)
                                GCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCA                                                                                                                     <  SRR19973069.3495551/150‑1 (MQ=60)
                                  GATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACG                                                                                                                   >  SRR19973069.1756902/1‑150 (MQ=60)
                                  GATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACG                                                                                                                   <  SRR19973069.82387/150‑1 (MQ=60)
                                  GATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACG                                                                                                                   >  SRR19973069.275171/1‑150 (MQ=60)
                                                CGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGC                                                                                                     <  SRR19973069.3577705/150‑1 (MQ=60)
                                                      CAACGATTTCCGTCATCTGACGATAGAAGATGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCG                                                                                               <  SRR19973069.546587/150‑1 (MQ=60)
                                                      CAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCG                                                                                               <  SRR19973069.126971/150‑1 (MQ=60)
                                                       AACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGC                                                                                              >  SRR19973069.2467478/1‑150 (MQ=60)
                                                            TTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGA                                                                                         <  SRR19973069.1233814/150‑1 (MQ=60)
                                                              TCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATA                                                                                       <  SRR19973069.3496357/150‑1 (MQ=60)
                                                              TCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATA                                                                                       <  SRR19973069.3495256/150‑1 (MQ=60)
                                                                    ATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTC                                                                                 <  SRR19973069.1089551/150‑1 (MQ=60)
                                                                    ATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTC                                                                                 <  SRR19973069.1089558/150‑1 (MQ=60)
                                                                    ATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTC                                                                                 <  SRR19973069.3049470/150‑1 (MQ=60)
                                                                     TCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCG                                                                                <  SRR19973069.1091856/150‑1 (MQ=60)
                                                                          CGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACT                                                                           >  SRR19973069.3788566/1‑150 (MQ=60)
                                                                          CGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACT                                                                           >  SRR19973069.3788570/1‑150 (MQ=60)
                                                                          CGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACT                                                                           >  SRR19973069.2707936/1‑150 (MQ=60)
                                                                             TAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCC                                                                        <  SRR19973069.3467271/150‑1 (MQ=60)
                                                                               GAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTG                                                                      >  SRR19973069.1326828/1‑150 (MQ=60)
                                                                                AAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGA                                                                     >  SRR19973069.1450066/1‑150 (MQ=60)
                                                                                AAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGA                                                                     >  SRR19973069.1449945/1‑150 (MQ=60)
                                                                                    AGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAG                                                                 >  SRR19973069.1187485/1‑150 (MQ=60)
                                                                                    AGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAG                                                                 <  SRR19973069.1153848/150‑1 (MQ=60)
                                                                                    AGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAG                                                                 <  SRR19973069.957037/150‑1 (MQ=60)
                                                                                       TCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGA                                                              <  SRR19973069.2326538/150‑1 (MQ=60)
                                                                                        CAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAG                                                             >  SRR19973069.1332325/1‑150 (MQ=60)
                                                                                         AACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGC                                                            >  SRR19973069.605531/1‑150 (MQ=60)
                                                                                         AACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGC                                                            >  SRR19973069.1656348/1‑150 (MQ=60)
                                                                                           CAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCAT                                                          <  SRR19973069.2462750/150‑1 (MQ=60)
                                                                                            AGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATC                                                         <  SRR19973069.2280132/150‑1 (MQ=60)
                                                                                             GCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCT                                                        <  SRR19973069.3466386/150‑1 (MQ=60)
                                                                                             GCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCT                                                        <  SRR19973069.3466432/150‑1 (MQ=60)
                                                                                             GCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCT                                                        <  SRR19973069.1797469/150‑1 (MQ=60)
                                                                                              CAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTT                                                       <  SRR19973069.1476264/150‑1 (MQ=60)
                                                                                               AGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTA                                                      >  SRR19973069.2246474/1‑150 (MQ=60)
                                                                                                 CGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATC                                                    >  SRR19973069.3305300/1‑150 (MQ=60)
                                                                                                  GTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCG                                                   >  SRR19973069.3227630/1‑150 (MQ=60)
                                                                                                      GAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGC                                               >  SRR19973069.1142402/1‑150 (MQ=60)
                                                                                                      GAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGC                                               >  SRR19973069.3169634/1‑150 (MQ=60)
                                                                                                      GAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGC                                               >  SRR19973069.1142297/1‑150 (MQ=60)
                                                                                                               AGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCT                                      >  SRR19973069.2782199/1‑150 (MQ=60)
                                                                                                               AGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCT                                      >  SRR19973069.636835/1‑150 (MQ=60)
                                                                                                               AGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCT                                      >  SRR19973069.2566457/1‑150 (MQ=60)
                                                                                                                  CGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGA                                   >  SRR19973069.1525662/1‑150 (MQ=60)
                                                                                                                      GAGGTCCGCATCGGTCATGTTTATTATGCAGTTTTAACGCAGTTTGTCCGGGTTGTACTGGTCACGACCGATACCACAGCCAAGCGCGGTGATTAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACTTT                               <  SRR19973069.151828/150‑1 (MQ=60)
                                                                                                                      GACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTT                               >  SRR19973069.3205763/1‑150 (MQ=60)
                                                                                                                            CGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAT                         <  SRR19973069.1151774/150‑1 (MQ=60)
                                                                                                                              CATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTT                       <  SRR19973069.1857553/150‑1 (MQ=60)
                                                                                                                               ATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTT                      <  SRR19973069.2291137/150‑1 (MQ=60)
                                                                                                                                 CGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAC                    <  SRR19973069.309805/150‑1 (MQ=60)
                                                                                                                                   GTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCC                  >  SRR19973069.132106/1‑150 (MQ=60)
                                                                                                                                   GTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCC                  >  SRR19973069.2448409/1‑150 (MQ=60)
                                                                                                                                   GTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCC                  <  SRR19973069.854370/150‑1 (MQ=60)
                                                                                                                                   GTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCC                  >  SRR19973069.2974839/1‑150 (MQ=60)
                                                                                                                                   GTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCC                  >  SRR19973069.2975096/1‑150 (MQ=60)
                                                                                                                                      ATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTagatcggaagagcacacgtctga               >  SRR19973069.2537090/1‑127 (MQ=60)
                                                                                                                                      ATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTC               >  SRR19973069.3423455/1‑150 (MQ=60)
                                                                                                                                      ATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTC               >  SRR19973069.3461357/1‑150 (MQ=60)
                                                                                                                                       TGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCA              >  SRR19973069.3583593/1‑150 (MQ=60)
                                                                                                                                       TGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCA              <  SRR19973069.3330545/150‑1 (MQ=60)
                                                                                                                                       TGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTGGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCA              >  SRR19973069.3581160/1‑150 (MQ=60)
                                                                                                                                          TGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCG           >  SRR19973069.3315184/1‑150 (MQ=60)
                                                                                                                                                  CAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATC   >  SRR19973069.1953126/1‑150 (MQ=60)
                                                                                                                                                  CAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATC   <  SRR19973069.1202423/150‑1 (MQ=60)
                                                                                                                                                   AGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCG  >  SRR19973069.1033275/1‑150 (MQ=60)
                                                                                                                                                   AGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCG  <  SRR19973069.3003169/150‑1 (MQ=60)
                                                                                                                                                   AGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCG  <  SRR19973069.3004158/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
TGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCG  >  CP009273/3871859‑3872155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑