Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,880,731 G→T 100% D182Y (GAT→TAT)  mnmE → tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,880,7310GT99.6% 796.3 / ‑4.4 238D182Y (GAT→TAT) mnmEtRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (95/142);  total (96/142)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 238 total aligned reads displayed.

CGACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCGATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAAT  >  CP009273/3880586‑3880875
                                                                                                                                                 |                                                                                                                                                
cGACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTAt                                                                                                                                                >  1:3373246/1‑148 (MQ=255)
 gACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTAtt                                                                                                                                               <  1:3026130/148‑1 (MQ=255)
 gACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTAtt                                                                                                                                               <  2:3564974/148‑1 (MQ=255)
   cGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTc                                                                                                                                             <  2:1008094/148‑1 (MQ=255)
    gCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTcc                                                                                                                                            <  1:1438093/148‑1 (MQ=255)
       aGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCctct                                                                                                                                         <  1:3464849/148‑1 (MQ=255)
          tCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCg                                                                                                                                      <  1:1175296/148‑1 (MQ=255)
                cAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGaa                                                                                                                                >  1:3264105/1‑148 (MQ=255)
                  ggcggcCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGaaaa                                                                                                                              <  2:1764993/148‑1 (MQ=255)
                       cccGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGa                                                                                                                         <  1:2633040/148‑1 (MQ=255)
                       cccGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGa                                                                                                                         >  2:1271478/1‑148 (MQ=255)
                         cGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAg                                                                                                                       <  2:3234911/148‑1 (MQ=255)
                          gTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGc                                                                                                                      <  1:790399/148‑1 (MQ=255)
                           ttCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGcc                                                                                                                     <  1:1595842/148‑1 (MQ=255)
                           ttCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGcc                                                                                                                     >  2:1815718/1‑148 (MQ=255)
                                  cTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCa                                                                                                              <  2:2863411/148‑1 (MQ=255)
                                  cTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCa                                                                                                              >  1:2359093/1‑148 (MQ=255)
                                   ttAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCaa                                                                                                             <  2:3395586/148‑1 (MQ=255)
                                      aCTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGa                                                                                                          >  2:65910/1‑148 (MQ=255)
                                       cTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGTCGGAAAAATTGAAGCCCAGCTCAATGAc                                                                                                         >  2:1950423/1‑148 (MQ=255)
                                       cTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGAc                                                                                                         >  2:727579/1‑148 (MQ=255)
                                           cTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTa                                                                                                     >  2:1177493/1‑148 (MQ=255)
                                           cTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTa                                                                                                     <  2:1772198/148‑1 (MQ=255)
                                           cTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTa                                                                                                     <  2:394468/148‑1 (MQ=255)
                                           cTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTa                                                                                                     <  1:3082814/148‑1 (MQ=255)
                                           cTGCAAGGCGCATTCTCCGCACGGGTTAATCATCGGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTa                                                                                                     <  2:2386296/148‑1 (MQ=255)
                                            tGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTAt                                                                                                    <  2:3336441/148‑1 (MQ=255)
                                            tGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTAt                                                                                                    <  1:2144131/148‑1 (MQ=255)
                                                 ggCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCg                                                                                               >  2:73819/1‑148 (MQ=255)
                                                    gcATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATc                                                                                            <  1:3406677/148‑1 (MQ=255)
                                                     cATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCt                                                                                           >  2:1517850/1‑148 (MQ=255)
                                                       ttCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTg                                                                                         <  2:777985/148‑1 (MQ=255)
                                                       ttCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTg                                                                                         <  2:464286/148‑1 (MQ=255)
                                                       ttCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTg                                                                                         <  2:2694253/148‑1 (MQ=255)
                                                        tctcCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGa                                                                                        >  1:3144362/1‑148 (MQ=255)
                                                        tctcCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGa                                                                                        >  1:128251/1‑148 (MQ=255)
                                                        tctcCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGa                                                                                        <  1:2126447/148‑1 (MQ=255)
                                                        tctcCGCACGGGTCAACCATCTGGTGGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCTGATGAAGAGATCGATTTCCTCTCCGACGGCAAAATTGAAGCCCAGCTTAATGACGTGATTGCCGATCTCGa                                                                                        >  1:709527/1‑148 (MQ=255)
                                                           ccGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGc                                                                                     <  2:3775197/148‑1 (MQ=255)
                                                                cGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAgtgc                                                                                <  1:3092644/148‑1 (MQ=255)
                                                                     tAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCt                                                                           >  1:2543238/1‑148 (MQ=255)
                                                                     tAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCt                                                                           >  1:3683533/1‑148 (MQ=255)
                                                                      aaTCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTg                                                                          <  2:2608801/148‑1 (MQ=255)
                                                                       atcatcTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGa                                                                         >  1:3812623/1‑148 (MQ=255)
                                                                         catcTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAg                                                                       >  1:1122312/1‑148 (MQ=255)
                                                                         catcTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAg                                                                       <  1:964091/148‑1 (MQ=255)
                                                                         catcTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAg                                                                       <  1:167655/148‑1 (MQ=255)
                                                                         catcTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAg                                                                       >  2:1386659/1‑148 (MQ=255)
                                                                         catcTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATGTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAg                                                                       <  1:2097659/148‑1 (MQ=255)
                                                                           tcTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCa                                                                     <  2:848525/148‑1 (MQ=255)
                                                                              ggTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGt                                                                  >  2:1868817/1‑148 (MQ=255)
                                                                               gTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTc                                                                 <  1:635678/148‑1 (MQ=255)
                                                                                  gAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAgg                                                              >  2:3514999/1‑148 (MQ=255)
                                                                                  gAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAgg                                                              >  2:3511486/1‑148 (MQ=255)
                                                                                  gAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATGTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAgg                                                              <  2:2059680/148‑1 (MQ=255)
                                                                                  gAAGCCCTCACCCACTTGCGCAGTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAgg                                                              <  2:129860/148‑1 (MQ=255)
                                                                                   aaGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCTATCTTGATGCAGTGCGTGCTGAAGCACGTCAggg                                                             >  1:2210025/1‑148 (MQ=255)
                                                                                      cccTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAg                                                          >  1:3054557/1‑148 (MQ=255)
                                                                                       ccTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGt                                                         <  2:3715262/148‑1 (MQ=255)
                                                                                       ccTCACCCACGTGCGCAGTTACGACGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGt                                                         <  2:2048371/148‑1 (MQ=255)
                                                                                         tCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGttt                                                       >  2:364496/1‑148 (MQ=255)
                                                                                          cACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTttg                                                      <  1:566265/148‑1 (MQ=255)
                                                                                          cACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTttg                                                      <  1:2380847/148‑1 (MQ=255)
                                                                                           aCCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTttgt                                                     >  1:1973421/1‑148 (MQ=255)
                                                                                              cACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTgc                                                  >  1:2377318/1‑148 (MQ=255)
                                                                                               aCTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTgcg                                                 >  2:1793153/1‑148 (MQ=255)
                                                                                                cTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATATTGATTCAGTGCGTGCTGAAGCACTTCAGGGTAGTTTGTTgctc                                                >  2:2502860/1‑146 (MQ=255)
                                                                                                  tGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGa                                              <  1:1813292/148‑1 (MQ=255)
                                                                                                  tGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGa                                              <  2:2472606/148‑1 (MQ=255)
                                                                                                   gcgcATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGaa                                             <  2:1098639/148‑1 (MQ=255)
                                                                                                       aTTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGa                                         <  1:3686109/148‑1 (MQ=255)
                                                                                                        tttACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGAt                                        <  2:3072791/148‑1 (MQ=255)
                                                                                                          tACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGa                                      >  2:2768355/1‑148 (MQ=255)
                                                                                                            cGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGaaa                                    <  2:1265661/148‑1 (MQ=255)
                                                                                                             gTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAg                                   <  1:3497253/148‑1 (MQ=255)
                                                                                                              tCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAgt                                  <  1:1177493/148‑1 (MQ=255)
                                                                                                              tCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAgt                                  >  2:664608/1‑148 (MQ=255)
                                                                                                               cGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAgtg                                 <  2:1077201/148‑1 (MQ=255)
                                                                                                               cGAAGCGGCAATTGATTGCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAgtg                                 <  2:1805631/148‑1 (MQ=255)
                                                                                                                  aGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAgtggtg                              <  2:529133/148‑1 (MQ=255)
                                                                                                                  aGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAgtggtg                              <  2:2282881/148‑1 (MQ=255)
                                                                                                                   gCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGa                             <  2:3259698/148‑1 (MQ=255)
                                                                                                                   gCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGa                             <  1:65910/148‑1 (MQ=255)
                                                                                                                    cGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGAt                            <  2:1030621/148‑1 (MQ=255)
                                                                                                                      gCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTg                          <  1:2761370/148‑1 (MQ=255)
                                                                                                                           tGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGa                     >  1:600886/1‑148 (MQ=255)
                                                                                                                             aTTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAGAGTGGTGATTGCCGGACg                   >  2:2205200/1‑148 (MQ=255)
                                                                                                                             aTTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACg                   >  2:1673375/1‑148 (MQ=255)
                                                                                                                              tttCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGt                  >  1:1366218/1‑148 (MQ=255)
                                                                                                                               ttCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTc                 <  2:1510261/148‑1 (MQ=255)
                                                                                                                                  cccGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTa              <  1:1029964/148‑1 (MQ=255)
                                                                                                                                  cccGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTa              >  2:2589900/1‑148 (MQ=255)
                                                                                                                                   ccGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTaa             <  1:577729/148‑1 (MQ=255)
                                                                                                                                        gAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGAGGAAAGTGGTGATTGCCGGACGTCCTAACGCCg        >  2:426457/1‑148 (MQ=255)
                                                                                                                                        gAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGAGTGCTGAAGCACGTCAGGGGAGTTGTTTGCGCGAAGGGATGAAAGTGGTGATTGCCTGACGTCCTAACTCCg        >  1:671144/1‑148 (MQ=255)
                                                                                                                                         aaGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCATTGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTTGTTTGTTGCGCGAAGGGTTGAAAGTGGTGTTTGCCGGACGTCCTAACGCCgg       <  1:1107849/148‑1 (MQ=255)
                                                                                                                                             gaTCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTaaa   <  2:3165291/148‑1 (MQ=255)
                                                                                                                                             gaTCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTaaa   <  2:2288969/148‑1 (MQ=255)
                                                                                                                                             gaTCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTaaa   >  2:890966/1‑148 (MQ=255)
                                                                                                                                              aTCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAAt  >  1:944189/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCGATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAAT  >  CP009273/3880586‑3880875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTATCGACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCGATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAATCGAG  >  CP009273/3880582‑3880879
                                                                                                                                                     |                                                                                                                                                    
TTATCGACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGTCGCATTCTCCGCACGGGTTAATCATCTCGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGCCAATAGATTTCCCCGATGAAGAGATCT                                                                                                                                                      >  SRR19973069.166179/1‑150 (MQ=60)
  ATCGACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTAT                                                                                                                                                    >  SRR19973069.3395303/1‑150 (MQ=60)
     GACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTC                                                                                                                                                 >  SRR19973069.1037759/1‑150 (MQ=60)
     GACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTC                                                                                                                                                 <  SRR19973069.3046199/150‑1 (MQ=60)
        GCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTC                                                                                                                                              <  SRR19973069.1448128/150‑1 (MQ=60)
         CCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCT                                                                                                                                             <  SRR19973069.83619/150‑1 (MQ=60)
          CAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTC                                                                                                                                            <  SRR19973069.2842660/150‑1 (MQ=60)
          CAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTC                                                                                                                                            <  SRR19973069.2842631/150‑1 (MQ=60)
           AGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCC                                                                                                                                           <  SRR19973069.3487273/150‑1 (MQ=60)
              TCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGAC                                                                                                                                        <  SRR19973069.1183373/150‑1 (MQ=60)
              TCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGAC                                                                                                                                        <  SRR19973069.1183434/150‑1 (MQ=60)
              TCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGAC                                                                                                                                        <  SRR19973069.1183501/150‑1 (MQ=60)
                  AACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAA                                                                                                                                    >  SRR19973069.3285488/1‑150 (MQ=60)
                      GGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAAT                                                                                                                                <  SRR19973069.3103291/150‑1 (MQ=60)
                           CCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTTCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAG                                                                                                                           >  SRR19973069.991259/1‑150 (MQ=60)
                           CCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAG                                                                                                                           <  SRR19973069.2650676/150‑1 (MQ=60)
                              GTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCC                                                                                                                        <  SRR19973069.796012/150‑1 (MQ=60)
                               TTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCA                                                                                                                       <  SRR19973069.1606614/150‑1 (MQ=60)
                               TTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCA                                                                                                                       <  SRR19973069.1606631/150‑1 (MQ=60)
                                TCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAG                                                                                                                      <  SRR19973069.33148/150‑1 (MQ=60)
                                    CACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCATTTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCA                                                                                                                  <  SRR19973069.3085150/150‑1 (MQ=60)
                                    CACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCA                                                                                                                  >  SRR19973069.2375118/1‑150 (MQ=60)
                                    CACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCA                                                                                                                  >  SRR19973069.2375203/1‑150 (MQ=60)
                                    CACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCA                                                                                                                  <  SRR19973069.3085241/150‑1 (MQ=60)
                                        TAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGA                                                                                                              >  SRR19973069.2451810/1‑150 (MQ=60)
                                              GCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTAT                                                                                                        <  SRR19973069.3243447/150‑1 (MQ=60)
                                               CTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATT                                                                                                       >  SRR19973069.2010331/1‑150 (MQ=60)
                                               CTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATT                                                                                                       <  SRR19973069.3103144/150‑1 (MQ=60)
                                                TGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTG                                                                                                      <  SRR19973069.2158849/150‑1 (MQ=60)
                                                  CAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCC                                                                                                    <  SRR19973069.2623887/150‑1 (MQ=60)
                                                   AAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCG                                                                                                   <  SRR19973069.2459/150‑1 (MQ=60)
                                                   AAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCG                                                                                                   >  SRR19973069.613785/1‑150 (MQ=60)
                                                        GCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTT                                                                                              <  SRR19973069.3428889/150‑1 (MQ=60)
                                                        GCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTT                                                                                              <  SRR19973069.3429185/150‑1 (MQ=60)
                                                        GCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTT                                                                                              <  SRR19973069.3790384/150‑1 (MQ=60)
                                                          ATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGA                                                                                            >  SRR19973069.1248038/1‑150 (MQ=60)
                                                          ATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGA                                                                                            >  SRR19973069.3164932/1‑150 (MQ=60)
                                                          ATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGA                                                                                            >  SRR19973069.129452/1‑150 (MQ=60)
                                                          ATTCTCCGCACGGGTCAACCATCTGGTGGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCTGATGAAGAGATCGATTTCCTCTCCGACGGCAAAATTGAAGCCCAGCTTAATGACGTGATTGCCGATCTCGA                                                                                            >  SRR19973069.714509/1‑150 (MQ=60)
                                                            TCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATG                                                                                          <  SRR19973069.699956/150‑1 (MQ=60)
                                                            TCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATG                                                                                          <  SRR19973069.2141077/150‑1 (MQ=60)
                                                            TCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATG                                                                                          <  SRR19973069.198439/150‑1 (MQ=60)
                                                                    CGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGT                                                                                  <  SRR19973069.3113025/150‑1 (MQ=60)
                                                                     GGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTG                                                                                 >  SRR19973069.599308/1‑150 (MQ=60)
                                                                       GTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCT                                                                               >  SRR19973069.3707625/1‑150 (MQ=60)
                                                                       GTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCT                                                                               >  SRR19973069.2560063/1‑150 (MQ=60)
                                                                         TAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGA                                                                             >  SRR19973069.3837300/1‑150 (MQ=60)
                                                                          AATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAA                                                                            >  SRR19973069.426900/1‑150 (MQ=60)
                                                                           ATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAG                                                                           >  SRR19973069.1130137/1‑150 (MQ=60)
                                                                            TCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGC                                                                          >  SRR19973069.3185926/1‑150 (MQ=60)
                                                                             CATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCA                                                                         <  SRR19973069.970524/150‑1 (MQ=60)
                                                                             CATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCA                                                                         <  SRR19973069.169091/150‑1 (MQ=60)
                                                                             CATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCA                                                                         <  SRR19973069.2112301/150‑1 (MQ=60)
                                                                             CATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATGTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCA                                                                         <  SRR19973069.2112148/150‑1 (MQ=60)
                                                                              ATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCAC                                                                        <  SRR19973069.1462264/150‑1 (MQ=60)
                                                                              ATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCAC                                                                        <  SRR19973069.1462266/150‑1 (MQ=60)
                                                                                   GTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAG                                                                   <  SRR19973069.639864/150‑1 (MQ=60)
                                                                                    TAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCTGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGG                                                                  <  SRR19973069.2238969/150‑1 (MQ=60)
                                                                                     AGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCTATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGG                                                                 >  SRR19973069.2225001/1‑150 (MQ=60)
                                                                                     AGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGG                                                                 >  SRR19973069.3709091/1‑150 (MQ=60)
                                                                                       AAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTA                                                               <  SRR19973069.3400045/150‑1 (MQ=60)
                                                                                        AGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAG                                                              >  SRR19973069.3074686/1‑150 (MQ=60)
                                                                                        AGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAG                                                              >  SRR19973069.3074769/1‑150 (MQ=60)
                                                                                          CCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTT                                                            >  SRR19973069.2287392/1‑150 (MQ=60)
                                                                                           CCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTT                                                           <  SRR19973069.732719/150‑1 (MQ=60)
                                                                                             TCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGT                                                         >  SRR19973069.1987135/1‑150 (MQ=60)
                                                                                              CACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTT                                                        <  SRR19973069.570198/150‑1 (MQ=60)
                                                                                              CACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTT                                                        <  SRR19973069.2396992/150‑1 (MQ=60)
                                                                                              CACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTT                                                        <  SRR19973069.570444/150‑1 (MQ=60)
                                                                                                CCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGC                                                      >  SRR19973069.2393437/1‑150 (MQ=60)
                                                                                                CCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGC                                                      >  SRR19973069.2393649/1‑150 (MQ=60)
                                                                                                 CCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCG                                                     >  SRR19973069.2147723/1‑150 (MQ=60)
                                                                                                 CCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCG                                                     >  SRR19973069.2147722/1‑150 (MQ=60)
                                                                                                      TGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAG                                                <  SRR19973069.1824306/150‑1 (MQ=60)
                                                                                                      TGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAG                                                <  SRR19973069.1826034/150‑1 (MQ=60)
                                                                                                          CATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGAT                                            <  SRR19973069.1139389/150‑1 (MQ=60)
                                                                                                           ATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATG                                           <  SRR19973069.3710217/150‑1 (MQ=60)
                                                                                                              TACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAA                                        >  SRR19973069.547383/1‑150 (MQ=60)
                                                                                                                 GTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTG                                     <  SRR19973069.3519788/150‑1 (MQ=60)
                                                                                                                  TCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGG                                    <  SRR19973069.1185639/150‑1 (MQ=60)
                                                                                                                       GCGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATT                               <  SRR19973069.66674/150‑1 (MQ=60)
                                                                                                                       GCGGCAAGTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATT                               <  SRR19973069.66794/150‑1 (MQ=60)
                                                                                                                        CGGCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTG                              <  SRR19973069.3501691/150‑1 (MQ=60)
                                                                                                                          GCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCC                            >  SRR19973069.1310694/1‑150 (MQ=60)
                                                                                                                          GCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCC                            >  SRR19973069.1860913/1‑150 (MQ=60)
                                                                                                                          GCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCC                            <  SRR19973069.2779800/150‑1 (MQ=60)
                                                                                                                          GCAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCC                            >  SRR19973069.1310874/1‑150 (MQ=60)
                                                                                                                          GCAATTGATGTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCC                            <  SRR19973069.65362/150‑1 (MQ=60)
                                                                                                                           CAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCG                           <  SRR19973069.3597302/150‑1 (MQ=60)
                                                                                                                           CAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCG                           <  SRR19973069.1396336/150‑1 (MQ=60)
                                                                                                                           CAATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCG                           <  SRR19973069.3597011/150‑1 (MQ=60)
                                                                                                                             ATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGA                         <  SRR19973069.2196041/150‑1 (MQ=60)
                                                                                                                             ATTGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGA                         >  SRR19973069.604934/1‑150 (MQ=60)
                                                                                                                               TGATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACG                       >  SRR19973069.3424163/1‑150 (MQ=60)
                                                                                                                                GATTTCCCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGT                      >  SRR19973069.1375719/1‑150 (MQ=60)
                                                                                                                                      CCCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAAC                <  SRR19973069.1037098/150‑1 (MQ=60)
                                                                                                                                       CCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACG               <  SRR19973069.581706/150‑1 (MQ=60)
                                                                                                                                       CCGATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACG               <  SRR19973069.581784/150‑1 (MQ=60)
                                                                                                                                          ATGAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGAGTGCTGAAGCACGTCAGGGGAGTTGTTTGCGCGAAGGGATGAAAGTGGTGATTGCCTGACGTCCTAACTCCG            >  SRR19973069.675759/1‑150 (MQ=60)
                                                                                                                                            GAAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGT          >  SRR19973069.668489/1‑150 (MQ=60)
                                                                                                                                             AAGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCATTGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTTGTTTGTTGCGCGAAGGGTTGAAAGTGGTGTTTGCCGGACGTCCTAACGCCGGTA         <  SRR19973069.1115577/150‑1 (MQ=60)
                                                                                                                                              AGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTCGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAA        >  SRR19973069.1121438/1‑150 (MQ=60)
                                                                                                                                              AGAGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAA        <  SRR19973069.2704804/150‑1 (MQ=60)
                                                                                                                                                AGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTCGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAAT      >  SRR19973069.883028/1‑150 (MQ=60)
                                                                                                                                                AGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAAT      <  SRR19973069.311402/150‑1 (MQ=60)
                                                                                                                                                AGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAAT      >  SRR19973069.950554/1‑150 (MQ=60)
                                                                                                                                                AGATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAAT      >  SRR19973069.951135/1‑150 (MQ=60)
                                                                                                                                                 GATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAATC     >  SRR19973069.3057809/1‑150 (MQ=60)
                                                                                                                                                 GATCTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAATC     >  SRR19973069.1786107/1‑150 (MQ=60)
                                                                                                                                                    CTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAATCGAG  >  SRR19973069.1212455/1‑150 (MQ=60)
                                                                                                                                                    CTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAATCGAG  >  SRR19973069.2968096/1‑150 (MQ=60)
                                                                                                                                                    CTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAATCGAG  >  SRR19973069.1212468/1‑150 (MQ=60)
                                                                                                                                                    CTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAATCGAG  >  SRR19973069.2361174/1‑150 (MQ=60)
                                                                                                                                                    CTATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAATCGAG  >  SRR19973069.424362/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TTATCGACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTAGAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCGATTTCCTCTCCGACGGAAAAATTGAAGCCCAGCTCAATGACGTTATTGCCGATCTTGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGCGCGAAGGGATGAAAGTGGTGATTGCCGGACGTCCTAACGCCGGTAAATCGAG  >  CP009273/3880582‑3880879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑