Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,875,148 C→A 29.4% A178A (GCG→GCT)  dnaN ← DNA polymerase III, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,875,1480CA29.4% 558.6 / 206.2 289A178A (GCG→GCT) dnaNDNA polymerase III, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (104/100);  new base A (33/52);  total (137/152)
Fisher's exact test for biased strand distribution p-value = 7.04e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

Only 100 of 297 total aligned reads displayed.

CCAACGTGGGCGCGAATGTTGTTGCTGCCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCAGGGT  >  CP009273/3874999‑3875297
                                                                                                                                                     |                                                                                                                                                     
  aaCGTGGGCGCGAATGTTGTTGCTGCCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGc                                                                                                                                                     <  1:3087978/150‑1 (MQ=255)
  aaCGTGGGCGCGAATGTTGTTGCTGCCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGc                                                                                                                                                     <  1:4390995/150‑1 (MQ=255)
     gTGGGCGCGAATGTTGTTGCTGCCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAg                                                                                                                                                  >  2:721123/1‑150 (MQ=255)
     gTGGGCGCGAATGTTGTTGCTGCCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAg                                                                                                                                                  <  1:1528255/150‑1 (MQ=255)
       gggCGCGAATGTTGTTGCTGCCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGAc                                                                                                                                                <  1:1107153/150‑1 (MQ=255)
            cgAATGTTGTTGCTGCCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTgg                                                                                                                                           >  1:4524119/1‑150 (MQ=255)
            cgAATGTTGTTGCTGCCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTgg                                                                                                                                           >  2:125323/1‑150 (MQ=255)
                 gttgttGCTGCCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCACTCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTc                                                                                                                                      <  2:3848092/150‑1 (MQ=255)
                  ttgttgCTGCCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCg                                                                                                                                     >  1:4453520/1‑150 (MQ=255)
                    gttgCTGCCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGt                                                                                                                                   >  1:3007325/1‑150 (MQ=255)
                    gttgCTGCCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGt                                                                                                                                   >  2:804058/1‑150 (MQ=255)
                     ttgCTGCCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGtt                                                                                                                                  >  1:898113/1‑150 (MQ=255)
                     ttgCTGCCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGtt                                                                                                                                  <  1:5149072/150‑1 (MQ=255)
                      tgctgcCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTg                                                                                                                                 >  2:2416249/1‑150 (MQ=255)
                      tgctgcCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTg                                                                                                                                 <  2:4310943/150‑1 (MQ=255)
                            cAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACgg                                                                                                                           >  2:1119295/1‑150 (MQ=255)
                            cAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACgg                                                                                                                           >  1:4851496/1‑150 (MQ=255)
                            cAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACgg                                                                                                                           >  2:56649/1‑150 (MQ=255)
                              aTCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTg                                                                                                                         >  1:1577405/1‑150 (MQ=255)
                              aTCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTg                                                                                                                         <  1:3047629/150‑1 (MQ=255)
                               tCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTgc                                                                                                                        <  2:3670530/150‑1 (MQ=255)
                                cTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTgcg                                                                                                                       >  1:1990535/1‑150 (MQ=255)
                                cTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTgcg                                                                                                                       >  1:1015666/1‑150 (MQ=255)
                                cTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTgcg                                                                                                                       >  1:1015598/1‑150 (MQ=255)
                                  gTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCa                                                                                                                     >  2:1652194/1‑150 (MQ=255)
                                     cgcgCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGtt                                                                                                                  <  1:3206561/150‑1 (MQ=255)
                                      gcgcAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGttc                                                                                                                 <  1:3536076/150‑1 (MQ=255)
                                       cgcAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGttct                                                                                                                <  2:2547642/150‑1 (MQ=255)
                                        gcAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGttctt                                                                                                               <  1:1247828/150‑1 (MQ=255)
                                              gATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCtt                                                                                                         <  2:4774346/150‑1 (MQ=255)
                                                ttGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCg                                                                                                       <  1:1919144/150‑1 (MQ=255)
                                                 tGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCgg                                                                                                      >  1:2311324/1‑150 (MQ=255)
                                                    cgccgcCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGttt                                                                                                   <  2:3792010/150‑1 (MQ=255)
                                                    cgccgcCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGttt                                                                                                   <  1:1430578/150‑1 (MQ=255)
                                                    cgccgcCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCGTCGGttt                                                                                                   <  2:3063991/150‑1 (MQ=255)
                                                       cgccgTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCaa                                                                                                <  2:3937000/150‑1 (MQ=255)
                                                         ccgTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAAc                                                                                              <  2:210869/150‑1 (MQ=255)
                                                             cGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCa                                                                                          <  1:1144378/150‑1 (MQ=255)
                                                              gAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCAt                                                                                         >  2:173759/1‑150 (MQ=255)
                                                                gCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATAc                                                                                       <  2:5025768/150‑1 (MQ=255)
                                                                   tACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCAt                                                                                    >  1:2905111/1‑150 (MQ=255)
                                                                     cGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCAttt                                                                                  >  1:252594/1‑150 (MQ=255)
                                                                        aTCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAg                                                                               <  1:693536/150‑1 (MQ=255)
                                                                        aTCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAg                                                                               <  2:669844/150‑1 (MQ=255)
                                                                        aTCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAg                                                                               <  1:3497346/150‑1 (MQ=255)
                                                                          cAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTa                                                                             <  1:4363836/150‑1 (MQ=255)
                                                                          cAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTa                                                                             <  1:4961552/150‑1 (MQ=255)
                                                                           aGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTaa                                                                            >  2:1942546/1‑150 (MQ=255)
                                                                            gTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAAt                                                                           >  2:4879382/1‑150 (MQ=255)
                                                                             ttCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATa                                                                          <  1:4339986/150‑1 (MQ=255)
                                                                              tCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAg                                                                         <  2:2832501/150‑1 (MQ=255)
                                                                              tCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAg                                                                         <  2:1015665/150‑1 (MQ=255)
                                                                              tCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAg                                                                         <  2:2175760/150‑1 (MQ=255)
                                                                              tCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAg                                                                         <  1:5163605/150‑1 (MQ=255)
                                                                               cAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTCAGTAATAGc                                                                        >  2:3741255/1‑150 (MQ=255)
                                                                                 aTCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGa                                                                      >  2:5300935/1‑150 (MQ=255)
                                                                                   cACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAAc                                                                    <  1:5091010/150‑1 (MQ=255)
                                                                                    aCGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACg                                                                   <  2:1161135/150‑1 (MQ=255)
                                                                                        cTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCt                                                               <  2:3492215/150‑1 (MQ=255)
                                                                                           tACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGAt                                                            <  1:746249/150‑1 (MQ=255)
                                                                                             cgcgGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATgc                                                          <  2:2670607/150‑1 (MQ=255)
                                                                                                ggTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGcc                                                       <  1:1734916/150‑1 (MQ=255)
                                                                                                    cGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAg                                                   >  2:3412178/1‑150 (MQ=255)
                                                                                                     gATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGa                                                  >  1:3362738/1‑150 (MQ=255)
                                                                                                      aTCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGaa                                                 <  1:1272667/150‑1 (MQ=255)
                                                                                                      aTCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGaa                                                 >  2:5188019/1‑150 (MQ=255)
                                                                                                      aTCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTAAAACAGAATACAATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGaa                                                 >  1:1382582/1‑150 (MQ=255)
                                                                                                      aTCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGaa                                                 <  1:680296/150‑1 (MQ=255)
                                                                                                       tCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGaaa                                                >  2:1374821/1‑150 (MQ=255)
                                                                                                        cACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGaaaa                                               <  1:2801817/150‑1 (MQ=255)
                                                                                                        cACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGaaaa                                               >  1:3880330/1‑150 (MQ=255)
                                                                                                         aCCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAAc                                              <  2:1577405/150‑1 (MQ=255)
                                                                                                          ccGAATGGCTTNGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACt                                             >  1:2762094/1‑150 (MQ=255)
                                                                                                          ccGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACt                                             >  1:455571/1‑150 (MQ=255)
                                                                                                           cGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTg                                            <  2:4095185/150‑1 (MQ=255)
                                                                                                            gAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTgg                                           >  2:3082131/1‑150 (MQ=255)
                                                                                                            gAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTgg                                           >  2:4486740/1‑150 (MQ=255)
                                                                                                              aTGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGt                                         <  1:558038/150‑1 (MQ=255)
                                                                                                                  cTTGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCt                                     >  2:5039653/1‑150 (MQ=255)
                                                                                                                   ttGGCAAAGTTTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATATCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCtg                                    >  1:2701819/1‑149 (MQ=255)
                                                                                                                   ttGGCAAAGATTGACCAATTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCtt                                    >  2:356012/1‑150 (MQ=255)
                                                                                                                     ggCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCa                                  >  1:2242418/1‑150 (MQ=255)
                                                                                                                       cAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGGGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAAt                                <  1:3653880/150‑1 (MQ=255)
                                                                                                                        aaaGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATc                               <  1:4042307/150‑1 (MQ=255)
                                                                                                                               gACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGcg                        >  2:4538546/1‑149 (MQ=255)
                                                                                                                               gACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCt                        >  1:399196/1‑150 (MQ=255)
                                                                                                                                aCCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCtt                       <  2:2892881/150‑1 (MQ=255)
                                                                                                                                aCCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCtt                       <  2:2011454/150‑1 (MQ=255)
                                                                                                                                aCCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCtt                       >  2:3171126/1‑150 (MQ=255)
                                                                                                                                   aaTTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCAt                    >  1:534623/1‑150 (MQ=255)
                                                                                                                                   aaTTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCAt                    >  1:3776874/1‑150 (MQ=255)
                                                                                                                                    aTTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATc                   >  2:1297413/1‑150 (MQ=255)
                                                                                                                                    aTTGGCATTGAACAGACAGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATc                   <  2:4687040/150‑1 (MQ=255)
                                                                                                                                       ggCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGtt                <  1:4212892/150‑1 (MQ=255)
                                                                                                                                             gAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCTTCGTTGCCTGc          >  1:181481/1‑150 (MQ=255)
                                                                                                                                             gAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGc          >  1:614354/1‑150 (MQ=255)
                                                                                                                                                 aGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCa      >  1:4763796/1‑150 (MQ=255)
                                                                                                                                                    ccGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCAggg   <  1:2788529/150‑1 (MQ=255)
                                                                                                                                                    ccGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCAggg   <  1:362676/150‑1 (MQ=255)
                                                                                                                                                    ccGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCAggg   <  1:3117431/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCAACGTGGGCGCGAATGTTGTTGCTGCCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCAGGGT  >  CP009273/3874999‑3875297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A