Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,870,295 A→T 23.6% I6I (ATT→ATA)  yidA ← sugar phosphate phosphatase; substrates include erythrose 4‑P and mannose 1‑P phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,870,2950AT23.6% 754.0 / 256.3 343I6I (ATT→ATA) yidAsugar phosphate phosphatase; substrates include erythrose 4‑P and mannose 1‑P phosphatase
Reads supporting (aligned to +/- strand):  ref base A (113/149);  new base T (37/44);  total (150/193)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 350 total aligned reads displayed.

TCTTTCAGGTAGTTGTGCACACCTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCC  >  CP009273/3870146‑3870443
                                                                                                                                                     |                                                                                                                                                    
tctTTCAGGTAGTTGTGCACACCTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCa                                                                                                                                                      <  2:3146394/150‑1 (MQ=255)
 ctTTCAGGTAGTTGTGCACACCTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCaa                                                                                                                                                     >  1:2563971/1‑150 (MQ=255)
   ttCAGGTAGTTGTGCACACCTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATg                                                                                                                                                   >  1:3112668/1‑150 (MQ=255)
    tCAGGTAGTTGTGCACACCTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGa                                                                                                                                                  >  1:48035/1‑150 (MQ=255)
     cAGGTAGTTGTGCACACCTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAg                                                                                                                                                 >  2:1499845/1‑150 (MQ=255)
      aGGTAGTTGTGCACACCTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGt                                                                                                                                                >  1:3214519/1‑150 (MQ=255)
       ggTAGTTGTGCACACCTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGtt                                                                                                                                               >  2:1697409/1‑150 (MQ=255)
        gTAGTTGTGCACACCTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGTGGTGATCGGGCCCCGGAAGTGTGCCAACCATATCGTAAGCAATGAGttt                                                                                                                                              >  1:1685848/1‑150 (MQ=255)
           gTTGTGCACACCTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCTATGAGTTTAAt                                                                                                                                           <  1:4634544/150‑1 (MQ=255)
           gTTGTGCACACCTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAAt                                                                                                                                           <  2:167319/150‑1 (MQ=255)
            ttGTGCACCCCTGCATACGGGCGACCCGTCGTTAGCACGACTTTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTCACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATa                                                                                                                                          <  1:96485/150‑1 (MQ=255)
            ttGTGCACACCTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATa                                                                                                                                          <  1:4311629/150‑1 (MQ=255)
             tgtgCACACCTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAg                                                                                                                                         <  1:1000903/150‑1 (MQ=255)
                 cacaCCTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCTATGAGTTTAATAGCCAt                                                                                                                                     <  2:4839266/150‑1 (MQ=255)
                 cacaCCTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCAt                                                                                                                                     <  2:1049686/150‑1 (MQ=255)
                  acacCTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAAAGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATATCAATGAGTTTAATAGCCATg                                                                                                                                    >  1:1947343/1‑150 (MQ=255)
                   cacCTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCTATGAGGTTAATAGCCATGa                                                                                                                                   >  2:2893649/1‑150 (MQ=255)
                    acCTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGGGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAg                                                                                                                                  >  1:3919800/1‑150 (MQ=255)
                     ccTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCCTATCGATAGCTATGAGTTTAATAGCCAGGAGt                                                                                                                                 >  2:52465/1‑150 (MQ=255)
                     ccTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGt                                                                                                                                 <  1:4733934/150‑1 (MQ=255)
                      cTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGtt                                                                                                                                <  1:4028395/150‑1 (MQ=255)
                      cTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGtt                                                                                                                                <  1:1569363/150‑1 (MQ=255)
                      cTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGtt                                                                                                                                <  2:4105044/150‑1 (MQ=255)
                      cTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGtt                                                                                                                                >  2:4222270/1‑150 (MQ=255)
                       tGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCTATGAGTTTAATAGCCATGAGTTc                                                                                                                               >  1:2500663/1‑150 (MQ=255)
                          aTACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCTATGAGTTTAATAGCCATGAGTTcccc                                                                                                                            <  2:1713310/150‑1 (MQ=255)
                           tACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCa                                                                                                                           >  1:2330427/1‑150 (MQ=255)
                           tACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCa                                                                                                                           <  2:1579721/150‑1 (MQ=255)
                                gCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAg                                                                                                                      <  1:1129036/150‑1 (MQ=255)
                                gCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAg                                                                                                                      >  2:248695/1‑150 (MQ=255)
                                  gACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGAt                                                                                                                    >  1:325733/1‑150 (MQ=255)
                                      cgtcgtTAGCACGACATTCACGCAACGGGCGCGAGCTGCTGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCTATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGt                                                                                                                >  1:3086641/1‑150 (MQ=255)
                                      cgtcgtGAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGt                                                                                                                <  2:3865957/150‑1 (MQ=255)
                                       gtcgtTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGtt                                                                                                               >  1:4517797/1‑150 (MQ=255)
                                       gtcgtTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGtt                                                                                                               >  1:2908319/1‑150 (MQ=255)
                                       gtcgtTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCAGTGTTAACGGCGGGTGAAATGGTGTGATCGGGCAGAAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGtt                                                                                                               >  1:2112980/1‑150 (MQ=255)
                                          gtTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCCGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCt                                                                                                            >  2:4705986/1‑150 (MQ=255)
                                          gtTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCt                                                                                                            <  1:276942/150‑1 (MQ=255)
                                          gtTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGACAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTCGATTGGTTCCt                                                                                                            >  2:652200/1‑150 (MQ=255)
                                            tAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGa                                                                                                          >  1:1834423/1‑150 (MQ=255)
                                               cACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCTATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGAccc                                                                                                       <  1:2233273/150‑1 (MQ=255)
                                                aCGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCa                                                                                                      <  1:2949601/150‑1 (MQ=255)
                                                 cGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCAt                                                                                                     <  2:2962740/150‑1 (MQ=255)
                                                 cGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCAt                                                                                                     >  1:3285710/1‑150 (MQ=255)
                                                    cATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCt                                                                                                  >  1:862386/1‑150 (MQ=255)
                                                     aTTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTa                                                                                                 <  2:1412343/150‑1 (MQ=255)
                                                     aTTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTa                                                                                                 <  2:2279545/150‑1 (MQ=255)
                                                      ttCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTaa                                                                                                >  2:3478691/1‑150 (MQ=255)
                                                       tCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAAc                                                                                               >  1:1609470/1‑150 (MQ=255)
                                                          cGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAAcgcg                                                                                            >  1:1396215/1‑150 (MQ=255)
                                                          cGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAAcgcg                                                                                            >  1:1666040/1‑150 (MQ=255)
                                                            ccACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGAt                                                                                          >  2:4488486/1‑150 (MQ=255)
                                                               cGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTcc                                                                                       <  1:298076/150‑1 (MQ=255)
                                                               cGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTcc                                                                                       <  1:2136618/150‑1 (MQ=255)
                                                                gggCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCTATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCg                                                                                      <  2:4102501/150‑1 (MQ=255)
                                                                gggCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCg                                                                                      >  1:509423/1‑150 (MQ=255)
                                                                gggCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCg                                                                                      <  2:3410335/150‑1 (MQ=255)
                                                                gggCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCg                                                                                      <  1:1933287/150‑1 (MQ=255)
                                                                     cgAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCaa                                                                                 <  2:914354/150‑1 (MQ=255)
                                                                     cgAGCTGCGGCAATCGAATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCaa                                                                                 >  1:4086988/1‑150 (MQ=255)
                                                                      gAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCaaa                                                                                >  1:3640518/1‑150 (MQ=255)
                                                                        gCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCaaaaa                                                                              >  2:3214975/1‑150 (MQ=255)
                                                                           gCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCTATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCa                                                                           <  2:768982/150‑1 (MQ=255)
                                                                           gCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCa                                                                           >  2:260199/1‑150 (MQ=255)
                                                                             ggCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCaaaa                                                                               >  2:4458129/1‑144 (MQ=255)
                                                                             ggCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCaaaa                                                                               <  1:4458129/144‑1 (MQ=255)
                                                                                 aTCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTacaa                                                                     >  1:3319065/1‑150 (MQ=255)
                                                                                   cGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTacaaca                                                                   >  2:954606/1‑150 (MQ=255)
                                                                                     cATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCTATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACAcc                                                                 >  1:622065/1‑150 (MQ=255)
                                                                                      aTTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCTGTACAACAccc                                                                >  2:1224704/1‑150 (MQ=255)
                                                                                      aTTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACAccc                                                                >  1:133004/1‑150 (MQ=255)
                                                                                         tttAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCTATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAg                                                             >  1:1755628/1‑150 (MQ=255)
                                                                                                   ggTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAgggg                                                   <  2:3920666/150‑1 (MQ=255)
                                                                                                    gTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAggggg                                                  >  1:2358589/1‑150 (MQ=255)
                                                                                                      gAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCTATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGAt                                                >  2:2063188/1‑150 (MQ=255)
                                                                                                           ggTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCTATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAAc                                           >  2:463873/1‑150 (MQ=255)
                                                                                                            gtgtGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAAcg                                          <  2:3349511/150‑1 (MQ=255)
                                                                                                                gATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCTATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAAcgcgcg                                      <  2:2975665/150‑1 (MQ=255)
                                                                                                                 aTCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGt                                     >  1:4228869/1‑150 (MQ=255)
                                                                                                                   cGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTg                                   <  2:673068/150‑1 (MQ=255)
                                                                                                                   cGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTg                                   <  2:2005348/150‑1 (MQ=255)
                                                                                                                    gggCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGc                                  <  2:269041/150‑1 (MQ=255)
                                                                                                                        agcagAAGGGTGCCATCCATATCGATAGCTATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGc                              <  2:3543940/150‑1 (MQ=255)
                                                                                                                        agcagAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGc                              >  2:3251811/1‑150 (MQ=255)
                                                                                                                         gcagAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCt                             <  2:2201930/150‑1 (MQ=255)
                                                                                                                             aaGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCCCt                         >  1:2198502/1‑150 (MQ=255)
                                                                                                                              aGGGTGCCATCCATATCGATAGCTATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCCCtt                        <  2:417840/150‑1 (MQ=255)
                                                                                                                              aGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCCCtt                        <  1:3672226/150‑1 (MQ=255)
                                                                                                                              aGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCCCtt                        <  1:192658/150‑1 (MQ=255)
                                                                                                                                ggTGCCATCCATATCGATAGCTATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCCCtttt                      <  1:3854375/150‑1 (MQ=255)
                                                                                                                                ggTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGGTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCCCtttt                      <  2:1318445/150‑1 (MQ=255)
                                                                                                                                 gTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCCCttttt                     <  2:2404318/150‑1 (MQ=255)
                                                                                                                                   gCCATCCATATCGATAGCTATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCCCTTTTTGc                   >  1:2786337/1‑150 (MQ=255)
                                                                                                                                     catccatATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCtt                 >  1:3947544/1‑150 (MQ=255)
                                                                                                                                         catATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCg             <  1:3759265/150‑1 (MQ=255)
                                                                                                                                          atatCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGATAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCgg            <  1:2923527/150‑1 (MQ=255)
                                                                                                                                           tatCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGa           >  2:1147627/1‑150 (MQ=255)
                                                                                                                                              cGATAGCAATGAGATTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCCTGCTAACGCGATTCCGCTCAAAAATCTGTACACCACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAaga        <  1:715661/150‑1 (MQ=255)
                                                                                                                                                  aGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGttt    <  2:48091/150‑1 (MQ=255)
                                                                                                                                                    cAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTcc  >  1:1502205/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCTTTCAGGTAGTTGTGCACACCTGCATACGGGCGACCCGTCGTTAGCACGACATTCACGCCACGGGCGCGAGCTGCGGCAATCGCATTTTTAACGGCGGGTGAAATGGTGTGATCGGGCAGCAGAAGGGTGCCATCCATATCGATAGCAATGAGTTTAATAGCCATGAGTTCCCCAGGTAGATTGGTTCCTGACCCATGCTAACGCGATTCCGCTCAAAAATCAGTACAACACCCGAGGGAAAAGGGGGATGCAACGCGCGTTGCGTGCTCCCTTTTTGCTTAGCGGAAGAGTTTCC  >  CP009273/3870146‑3870443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A