E. coli chemical tolerance: TOL adipic acid experiment stats

Notes

Publication:

Lennen RM, Jensen K, Mohammed ET, Malla S, Börner RA, Chekina K, et al. Adaptive laboratory evolution reveals general and specific chemical tolerance mechanisms and enhances biochemical production [Internet]. bioRxiv. 2019. p. 634105. doi:10.1101/634105

ALE, flask, and isolate counts

ALE replicate	Flask count	Isolate count
1	1	2
2	1	3
3	1	3
4	1	2
5	1	2
6	1	3
7	1	2
8	1	3
8	8	20

ALE experiment mutation counts

Mutation Type	Unique	Observed
Single Base Substitutions	417	472
Multiple Base Substitutions	0	0
Deletions	15	91
Insertions	5	43
Mobile Element Insertions	15	28
Amplifications	0	0
Gene Conversions	0	0
Inversions	0	0
Total	452	634

ALE experiment functional change counts

Functional Change Type	Unique	Observed
Intergenic	71	153
Noncoding	1	1
Pseudogene	10	12
Synonymous	0	0
Nonsynonymous	0	0
Total	82	166

Mutation Needle Plot

Mutation Targets