E. coli chemical tolerance: TOL putrescine experiment stats

Notes

Publication:

Lennen RM, Jensen K, Mohammed ET, Malla S, Börner RA, Chekina K, et al. Adaptive laboratory evolution reveals general and specific chemical tolerance mechanisms and enhances biochemical production [Internet]. bioRxiv. 2019. p. 634105. doi:10.1101/634105

ALE, flask, and isolate counts

ALE replicate	Flask count	Isolate count
1	1	2
2	1	3
3	1	3
4	1	3
5	1	3
6	1	3
7	1	3
7	7	20

ALE experiment mutation counts

Mutation Type	Unique	Observed
Single Base Substitutions	45	80
Multiple Base Substitutions	0	0
Deletions	21	83
Insertions	8	52
Mobile Element Insertions	7	12
Amplifications	0	0
Gene Conversions	0	0
Inversions	0	0
Total	81	227

ALE experiment functional change counts

Functional Change Type	Unique	Observed
Intergenic	20	86
Noncoding	0	0
Pseudogene	0	0
Synonymous	0	0
Nonsynonymous	0	0
Total	20	86

Mutation Needle Plot

Mutation Targets