C321: ECNR2.1 experiment stats

Notes

Publication:

Wannier TM, Kunjapur AM, Rice DP, McDonald MJ, Desai MM, Church GM. Adaptive evolution of genomically recoded Escherichia coli. Proc Natl Acad Sci U S A. 2018;115: 3090–3095.

ALE, flask, and isolate counts

ALE replicate	Flask count	Isolate count
0	1	1
1	1	2
2	1	2
3	1	2
4	1	2
5	1	2
6	1	2
7	1	2
8	1	2
9	1	2
10	1	2
11	1	2
12	1	2
13	1	2
14	1	2
15	15	29

ALE experiment mutation counts

Mutation Type	Unique	Observed
Single Base Substitutions	731	1607
Multiple Base Substitutions	1	1
Deletions	65	212
Insertions	96	261
Mobile Element Insertions	3	4
Amplifications	0	0
Gene Conversions	0	0
Inversions	0	0
Total	896	2085

ALE experiment functional change counts

Functional Change Type	Unique	Observed
Intergenic	118	293
Noncoding	15	24
Pseudogene	39	110
Synonymous	0	0
Nonsynonymous	0	0
Total	172	427

Mutation Needle Plot

Mutation Targets