breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 119,500 A→C intergenic (+157/‑4) pdhR → / → aceE pyruvate dehydrogenase complex transcriptional repressor PdhR/pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
MC JC 1,290,924 2651 bp→80 bp coding (4‑2654/2676 nt) adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
MC JC 1,749,959 1388 bp→80 bp coding (5‑1392/1413 nt) pykF → pyruvate kinase PykF
MC JC 1,931,909 1419 bp→81 bp coding (4‑1422/1443 nt) pyk → pyruvate kinase
RA 2,560,531 G→T N312K (AAC→AAA eutH ← ethanolamine utilization protein EutH
RA 3,081,838 G→A A66T (GCA→ACA)  galP → galactose/proton symporter
RA 4,140,482 G→T R849S (CGC→AGC)  ppc ← phosphoenolpyruvate carboxylase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 380208 380542 335 43 [41] [41] 43 BW25113_RS01880/tauA PIG‑L family deacetylase/taurine ABC transporter substrate‑binding protein
* * ÷ NZ_CP009273 578583 579176 594 42 [41] [41] 42 [BW25113_RS26085]–[appY] [BW25113_RS26085],[appY]
* * ÷ NZ_CP009273 946689 949098 2410 42 [41] [39] 48 pflB–[focA] pflB,[focA]
* * ÷ NZ_CP009273 1972971 1973149 179 112 [0] [37] 49 flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* * ÷ NZ_CP009273 3798057 3798583 527 42 [40] [40] 50 [rfaS]–[rfaP] [rfaS],[rfaP]
* * ÷ NZ_CP009273 4127838 4128981 1144 44 [38] [31] 46 gldA–[fsa] gldA,[fsa]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =69 (0.670)81 (0.780) 52/430 0.9 51.6% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 83 (0.800)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =45 (0.430)100 (1.040) 67/398 0.2 75.6% coding (290/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 1205075 = 23 (0.240)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 120326145 (0.430)68 (0.710) 51/398 0.7 67.8% coding (305/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 = 1205058 23 (0.240)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 1463497NA (NA)94 (0.910) 67/430 0.3 100% intergenic (‑91/‑118) BW25113_RS07355/BW25113_RS07365 IS3‑like element IS2 family transposase/IS30‑like element IS30 family transposase
?NZ_CP009273 1590687 = 0 (0.000)pseudogene (1657/5422 nt) BW25113_RS07915 ECSE_1600 family autotransporter
* ? NZ_CP009273 = 15906910 (0.000)110 (1.060) 78/430 0.1 100% pseudogene (1653/5422 nt) BW25113_RS07915 ECSE_1600 family autotransporter
?NZ_CP009273 = 1645805 NA (NA)intergenic (+11/‑3) BW25113_RS08260/BW25113_RS25635 IS3‑like element IS2 family transposase/tyrosine‑type recombinase/integrase
* ? NZ_CP009273 = 19729700 (0.000)112 (1.080) 77/430 0.1 100% intergenic (‑516/‑264) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP