Notes

Publication:

10.1101/634105

10.1016/j.ymben.2023.01.012

ALE, flask, and isolate counts

ALE replicate Flask count Isolate count
0 1 1
1 1 3
2 1 1
3 1 3
4 1 3
5 1 2
6 1 3
7 1 3
8 1 2
9 9 21

ALE experiment mutation counts

Mutation Type Unique Observed
Single Base Substitutions 22 59
Multiple Base Substitutions 2 3
Deletions 10 56
Insertions 4 6
Mobile Element Insertions 6 24
Amplifications 0 0
Gene Conversions 0 0
Inversions 0 0
Total 44 148

Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Unassigned Missing Coverage Evidence Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4 2 422.4 11956043 98.9% 11824526 164.0
A1 F50 I1 R1 12 4 61.5 1908208 99.4% 1896758 150.5
A1 F50 I2 R1 11 4 64.3 1995241 99.4% 1983269 150.5
A1 F50 I3 R1 12 4 65.6 2034438 99.5% 2024265 150.5
A2 F50 I1 R1 10 5 62.8 1944469 99.5% 1934746 150.5
A3 F50 I1 R1 11 4 63.1 1970573 98.9% 1948896 150.5
A3 F50 I2 R1 12 4 62.6 1936456 99.5% 1926773 150.5
A3 F50 I3 R1 12 4 59.6 1849432 99.4% 1838335 150.5
A4 F50 I1 R1 18 3 50.4 1572418 98.9% 1555121 150.4
A4 F50 I2 R1 12 3 66.3 2058453 99.4% 2046102 150.5
A4 F50 I3 R1 12 4 66.2 2053263 99.5% 2042996 150.5
A5 F50 I1 R1 13 4 62.8 1954599 99.3% 1940916 150.4
A5 F50 I2 R1 12 4 66.2 2059288 99.3% 2044872 150.4
A6 F50 I1 R1 13 5 52.4 1628453 99.2% 1615425 150.4
A6 F50 I2 R1 10 4 51.5 1603299 98.9% 1585662 150.4
A6 F50 I3 R1 19 5 48.5 1507762 99.1% 1494192 150.4
A7 F50 I1 R1 11 6 54.7 1701520 99.2% 1687907 150.4
A7 F50 I2 R1 11 5 42.5 1317570 99.2% 1307029 150.5
A7 F50 I3 R1 10 4 59.1 1843964 98.9% 1823680 150.5
A8 F50 I1 R1 11 4 64.6 2002243 99.4% 1990229 150.5
A8 F50 I2 R1 11 4 55.7 1728806 99.3% 1716704 150.5

Mutation Needle Plot

Mutation Targets

Entries to show