Notes

Publication:

10.1101/634105

10.1016/j.ymben.2023.01.012

ALE, flask, and isolate counts

ALE replicate Flask count Isolate count
0 1 1
1 1 1
2 1 3
3 1 3
4 1 3
5 1 3
6 1 3
7 1 2
8 1 2
9 9 21

ALE experiment mutation counts

Mutation Type Unique Observed
Single Base Substitutions 28 84
Multiple Base Substitutions 0 0
Deletions 15 51
Insertions 3 3
Mobile Element Insertions 11 21
Amplifications 0 0
Gene Conversions 0 0
Inversions 0 0
Total 57 159

Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Unassigned Missing Coverage Evidence Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4 2 422.4 11956043 98.9% 11824526 164.0
A1 F50 I1 R1 13 4 57.3 1801602 98.2% 1769173 150.3
A2 F50 I1 R1 11 3 49.7 1834398 98.7% 1810550 150.3
A2 F50 I2 R1 15 5 64.1 1999225 98.9% 1977233 150.3
A2 F50 I3 R1 11 4 55.0 1722361 98.6% 1698247 150.2
A3 F50 I1 R1 14 5 63.9 1999218 98.4% 1967230 150.3
A3 F50 I2 R1 13 5 61.3 1914960 98.8% 1891980 150.3
A3 F50 I3 R1 13 4 75.6 2362085 98.7% 2331377 150.3
A4 F50 I1 R1 10 3 70.5 2290837 95.9% 2196912 150.2
A4 F50 I2 R1 11 4 66.1 2134085 96.6% 2061526 150.2
A4 F50 I3 R1 9 5 85.7 2842947 99.0% 2814517 150.4
A5 F50 I1 R1 14 4 91.5 2893852 97.8% 2830187 150.2
A5 F50 I2 R1 11 3 78.5 2456180 98.8% 2426705 150.3
A5 F50 I3 R1 14 3 71.3 2231481 98.6% 2200240 150.3
A6 F50 I1 R1 12 3 71.9 2265445 98.3% 2226932 150.4
A6 F50 I2 R1 12 3 76.6 2571344 97.1% 2496775 150.1
A6 F50 I3 R1 14 4 62.1 2083843 98.4% 2050501 150.3
A7 F50 I1 R1 14 4 34.4 1090939 97.7% 1065847 150.2
A7 F50 I2 R1 12 3 55.0 1726278 98.4% 1698657 150.3
A8 F50 I1 R1 10 4 37.2 1161946 98.8% 1148002 150.4
A8 F50 I2 R1 11 6 25.2 794835 97.6% 775758 150.2

Mutation Needle Plot

Mutation Targets

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