Notes

Publication:

10.1101/634105

10.1016/j.ymben.2023.01.012

ALE, flask, and isolate counts

ALE replicate Flask count Isolate count
0 1 1
1 1 1
2 1 2
3 1 3
4 1 3
5 1 3
6 1 2
7 1 3
8 1 3
9 9 21

ALE experiment mutation counts

Mutation Type Unique Observed
Single Base Substitutions 203 334
Multiple Base Substitutions 1 2
Deletions 16 56
Insertions 8 10
Mobile Element Insertions 5 9
Amplifications 0 0
Gene Conversions 0 0
Inversions 0 0
Total 233 411

Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Unassigned Missing Coverage Evidence Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4 2 422.4 11956043 98.9% 11824526 164.0
A1 F50 I1 R1 12 4 81.2 2534277 98.8% 2503865 150.3
A2 F50 I1 R1 9 3 94.9 2959037 98.9% 2926487 150.4
A2 F50 I2 R1 9 3 89.0 2774222 99.0% 2746479 150.4
A3 F50 I1 R1 9 4 86.2 2679252 99.0% 2652459 150.4
A3 F50 I2 R1 11 5 77.6 2415865 98.9% 2389290 150.3
A3 F50 I3 R1 10 4 82.5 2565293 99.0% 2539640 150.4
A4 F50 I1 R1 50 4 78.3 2441730 98.9% 2414870 150.3
A4 F50 I2 R1 52 4 78.8 2524580 99.0% 2499334 150.4
A4 F50 I3 R1 49 3 90.3 2851930 97.9% 2792039 150.3
A5 F50 I1 R1 10 4 80.0 2494037 98.9% 2466602 150.3
A5 F50 I2 R1 9 3 76.6 2394727 98.5% 2358806 150.4
A5 F50 I3 R1 10 3 83.7 2612110 98.8% 2580764 150.3
A6 F50 I1 R1 96 5 83.8 2603420 99.1% 2579989 150.4
A6 F50 I2 R1 98 6 77.8 2424726 98.7% 2393204 150.3
A7 F50 I1 R1 9 4 82.3 2573520 98.7% 2540064 150.3
A7 F50 I2 R1 8 3 134.8 4192789 99.1% 4155053 150.4
A7 F50 I3 R1 9 4 75.5 2355322 98.9% 2329413 150.4
A8 F50 I1 R1 13 4 81.0 2526580 98.9% 2498787 150.3
A8 F50 I2 R1 11 4 85.7 2669942 99.0% 2643242 150.4
A8 F50 I3 R1 12 4 80.2 2501133 98.9% 2473620 150.3

Mutation Needle Plot

Mutation Targets

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