Notes

Publication:

10.1101/634105

10.1016/j.ymben.2023.01.012

ALE, flask, and isolate counts

ALE replicate Flask count Isolate count
0 1 1
1 1 3
2 1 3
3 1 3
4 1 3
5 1 4
6 1 1
7 1 3
8 8 21

ALE experiment mutation counts

Mutation Type Unique Observed
Single Base Substitutions 21 61
Multiple Base Substitutions 0 0
Deletions 8 32
Insertions 3 10
Mobile Element Insertions 10 16
Amplifications 0 0
Gene Conversions 0 0
Inversions 0 0
Total 42 119

Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Unassigned Missing Coverage Evidence Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4 2 422.4 11956043 98.9% 11824526 164.0
A1 F50 I1 R1 11 3 51.3 1592943 99.4% 1583385 150.4
A1 F50 I2 R1 11 4 69.3 2158313 99.1% 2138888 150.3
A1 F50 I3 R1 10 3 80.8 2504129 99.4% 2489104 150.4
A2 F50 I1 R1 9 3 75.3 2379883 99.6% 2370363 150.5
A2 F50 I2 R1 8 3 96.2 3136993 99.0% 3105623 150.4
A2 F50 I3 R1 8 4 81.9 2535443 99.4% 2520230 150.4
A3 F50 I1 R1 11 3 81.9 2535046 99.5% 2522370 150.4
A3 F50 I2 R1 11 4 63.5 1968565 99.3% 1954785 150.4
A3 F50 I3 R1 10 3 49.4 1534734 99.4% 1525525 150.4
A4 F50 I1 R1 10 3 80.8 2506062 99.0% 2481001 150.4
A4 F50 I2 R1 10 4 77.9 2391561 99.4% 2377211 150.4
A4 F50 I3 R1 9 3 85.6 2686842 99.5% 2673407 150.5
A5 F50 I1 R1 9 3 87.5 2719641 99.0% 2692444 150.4
A5 F50 I2 R1 8 4 82.8 2563995 99.5% 2551175 150.4
A5 F50 I3 R1 10 3 88.9 2753582 99.5% 2739814 150.4
A5 F50 I4 R1 10 4 62.5 1948381 99.5% 1938639 150.4
A6 F50 I1 R1 12 3 66.5 2076653 99.5% 2066269 150.4
A7 F50 I1 R1 13 5 66.1 2066055 99.2% 2049526 150.4
A7 F50 I2 R1 11 3 82.1 2539120 99.5% 2526424 150.4
A7 F50 I3 R1 12 4 74.4 2474678 99.6% 2464779 150.4

Mutation Needle Plot

Mutation Targets

Entries to show