Notes

Publication:

10.1101/634105

10.1016/j.ymben.2023.01.012

ALE, flask, and isolate counts

ALE replicate Flask count Isolate count
0 1 1
1 1 3
2 1 3
3 1 3
4 1 3
5 1 3
6 1 3
7 1 2
8 8 21

ALE experiment mutation counts

Mutation Type Unique Observed
Single Base Substitutions 151 331
Multiple Base Substitutions 0 0
Deletions 13 52
Insertions 17 33
Mobile Element Insertions 12 17
Amplifications 0 0
Gene Conversions 0 0
Inversions 0 0
Total 193 433

Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Unassigned Missing Coverage Evidence Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4 2 422.4 11956043 98.9% 11824526 164.0
A1 F50 I1 R1 10 3 63.0 1960998 99.0% 1941388 150.4
A1 F50 I2 R1 11 6 50.1 1602994 96.6% 1548492 150.2
A1 F50 I3 R1 9 4 65.6 2090459 96.9% 2025654 150.3
A2 F50 I1 R1 10 5 79.4 2510432 97.7% 2452692 150.3
A2 F50 I2 R1 8 5 43.8 1437549 94.1% 1352733 150.1
A2 F50 I3 R1 8 5 73.5 2346383 96.6% 2266605 150.3
A3 F50 I1 R1 8 3 55.4 1768540 97.7% 1727863 150.0
A3 F50 I2 R1 8 3 63.6 2046281 96.8% 1980800 150.0
A3 F50 I3 R1 8 3 70.6 2241301 97.0% 2174061 150.3
A4 F50 I1 R1 10 4 81.7 2677361 94.0% 2516719 150.1
A4 F50 I2 R1 11 3 63.7 2086091 94.4% 1969269 150.2
A4 F50 I3 R1 9 4 61.5 1934856 98.3% 1901963 150.3
A5 F50 I1 R1 121 5 78.3 2497591 96.8% 2417668 150.2
A5 F50 I2 R1 119 5 75.2 2391858 97.0% 2320102 150.3
A5 F50 I3 R1 122 5 62.2 2004626 95.6% 1916422 150.2
A6 F50 I1 R1 10 4 66.6 2095785 97.9% 2051773 150.3
A6 F50 I2 R1 11 4 62.0 1929183 99.2% 1913749 150.3
A6 F50 I3 R1 10 3 72.0 2263010 98.2% 2222275 150.3
A7 F50 I1 R1 7 3 76.1 2385381 98.4% 2347214 150.4
A7 F50 I2 R1 8 3 91.5 2843738 99.0% 2815300 150.4

Mutation Needle Plot

Mutation Targets

Entries to show