| ALE replicate | Flask count | Isolate count |
|---|---|---|
| 1 | 8 | 16 |
| 2 | 4 | 8 |
| 3 | 9 | 18 |
| 4 | 5 | 10 |
| 5 | 11 | 22 |
| 6 | 11 | 22 |
| 6 | 48 | 96 |
| Mutation Type | Unique | Observed |
|---|---|---|
| Single Base Substitutions | 332 | 1200 |
| Multiple Base Substitutions | 2 | 2 |
| Deletions | 116 | 424 |
| Insertions | 40 | 140 |
| Mobile Element Insertions | 171 | 525 |
| Amplifications | 12 | 32 |
| Gene Conversions | 4 | 15 |
| Inversions | 0 | 0 |
| Total | 677 | 2338 |
| ALE, Flask, Isolate | Predicted Mutations | Unassigned Missing Coverage Evidence | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|---|
| A1 F500 I1 R1 | 3 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A1 F500 I2 R1 | 5 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A1 F1000 I1 R1 | 4 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A1 F1000 I2 R1 | 6 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A1 F1500 I1 R1 | 5 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A1 F1500 I2 R1 | 7 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A1 F2000 I1 R1 | 6 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A1 F2000 I2 R1 | 7 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A1 F5000 I1 R1 | 15 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A1 F5000 I2 R1 | 15 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A1 F10000 I1 R1 | 27 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A1 F10000 I2 R1 | 28 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A1 F15000 I1 R1 | 36 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A1 F15000 I2 R1 | 36 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A1 F20000 I1 R1 | 44 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A1 F20000 I2 R1 | 42 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A2 F500 I1 R1 | 4 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A2 F500 I2 R1 | 3 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A2 F1000 I1 R1 | 5 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A2 F1000 I2 R1 | 4 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A2 F1500 I1 R1 | 5 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A2 F1500 I2 R1 | 5 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A2 F2000 I1 R1 | 6 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A2 F2000 I2 R1 | 5 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A3 F500 I1 R1 | 1 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A3 F500 I2 R1 | 4 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A3 F1000 I1 R1 | 7 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A3 F1000 I2 R1 | 5 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A3 F1500 I1 R1 | 8 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A3 F1500 I2 R1 | 6 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A3 F2000 I1 R1 | 10 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A3 F2000 I2 R1 | 11 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A3 F5000 I1 R1 | 17 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A3 F5000 I2 R1 | 17 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A3 F10000 I1 R1 | 27 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A3 F10000 I2 R1 | 30 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A3 F15000 I1 R1 | 38 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A3 F15000 I2 R1 | 39 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A3 F20000 I1 R1 | 48 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A3 F20000 I2 R1 | 53 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A3 F30000 I1 R1 | 55 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A3 F30000 I2 R1 | 73 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A4 F500 I1 R1 | 2 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A4 F500 I2 R1 | 2 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A4 F1000 I1 R1 | 4 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A4 F1000 I2 R1 | 3 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A4 F1500 I1 R1 | 7 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A4 F1500 I2 R1 | 8 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A4 F2000 I1 R1 | 9 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A4 F2000 I2 R1 | 8 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A4 F5000 I1 R1 | 14 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A4 F5000 I2 R1 | 13 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F500 I1 R1 | 3 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F500 I2 R1 | 3 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F1000 I1 R1 | 5 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F1000 I2 R1 | 4 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F1500 I1 R1 | 6 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F1500 I2 R1 | 7 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F2000 I1 R1 | 9 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F2000 I2 R1 | 9 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F5000 I1 R1 | 15 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F5000 I2 R1 | 14 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F10000 I1 R1 | 28 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F10000 I2 R1 | 36 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F15000 I1 R1 | 37 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F15000 I2 R1 | 35 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F20000 I1 R1 | 44 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F20000 I2 R1 | 44 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F30000 I1 R1 | 79 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F30000 I2 R1 | 65 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F40000 I1 R1 | 72 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F40000 I2 R1 | 75 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F50000 I1 R1 | 89 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A5 F50000 I2 R1 | 94 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F500 I1 R1 | 3 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F500 I2 R1 | 1 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F1000 I1 R1 | 4 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F1000 I2 R1 | 5 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F1500 I1 R1 | 6 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F1500 I2 R1 | 6 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F2000 I1 R1 | 8 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F2000 I2 R1 | 9 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F5000 I1 R1 | 12 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F5000 I2 R1 | 16 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F10000 I1 R1 | 24 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F10000 I2 R1 | 27 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F15000 I1 R1 | 34 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F15000 I2 R1 | 38 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F20000 I1 R1 | 43 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F20000 I2 R1 | 47 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F30000 I1 R1 | 61 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F30000 I2 R1 | 60 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F40000 I1 R1 | 81 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F40000 I2 R1 | 68 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F50000 I1 R1 | 93 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |
| A6 F50000 I2 R1 | 77 | 0 | 0.0 | 0 | 0.0% | 0 | 0.0 |