Notes

Publication:

10.1016/j.ymben.2016.11.008

10.1016/j.ymben.2016.11.008

10.1186/s12864-020-06920-4

ALE, flask, and isolate counts

ALE replicate Flask count Isolate count
0 1 1
3 1 2
5 1 2
6 1 2
8 1 2
9 1 2
11 1 2
7 7 13

ALE experiment mutation counts

Mutation Type Unique Observed
Single Base Substitutions 90 154
Multiple Base Substitutions 0 0
Deletions 9 33
Insertions 2 2
Mobile Element Insertions 6 25
Amplifications 0 0
Gene Conversions 0 0
Inversions 0 0
Total 107 214

Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Unassigned Missing Coverage Evidence Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 8 31 108.7 1897362 98.8% 1874593 250.2
A3 F50 I1 R1 10 7 129.8 4078390 99.0% 4037606 150.3
A3 F50 I2 R1 86 10 20.1 639881 99.2% 634761 149.7
A5 F50 I1 R1 17 9 39.6 1287126 99.2% 1276828 149.6
A5 F50 I2 R1 17 7 44.3 1742491 98.8% 1721581 148.9
A6 F50 I1 R1 17 8 88.0 2744638 98.9% 2714446 150.5
A6 F50 I2 R1 17 7 104.3 3234874 99.4% 3215464 150.6
A8 F50 I1 R1 19 8 40.1 1389605 98.5% 1368760 149.5
A8 F50 I2 R1 19 8 32.2 1105332 98.7% 1090962 149.1
A9 F50 I1 R1 19 8 27.7 945311 98.9% 934912 149.6
A9 F50 I2 R1 20 8 42.0 1472333 98.8% 1454665 149.3
A11 F1 I1 R1 11 9 91.9 2907140 98.1% 2851904 150.5
A11 F1 I2 R1 10 8 94.0 2939901 98.9% 2907562 150.5

Mutation Needle Plot

Mutation Targets

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