mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | HSM_end_HSM_A18_F91_I2_S141_L004_R1_001.good.fq | 3,065,002 | 304,821,105 | 100.0% | 99.5 bases | 101 bases | 97.6% |
| errors | HSM_end_HSM_A18_F91_I2_S141_L004_R2_001.good.fq | 3,065,002 | 304,821,105 | 100.0% | 99.5 bases | 101 bases | 96.8% |
| total | 6,130,004 | 609,642,210 | 100.0% | 99.5 bases | 101 bases | 97.2% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | JE3692-4 | 6,192,177 | 98.2 | 2.5 | 100.0% | Pseudomonas putida KT2440 complete genome. |
| total | 6,192,177 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 100000 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 2620 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.088 |
| reference sequence | pr(no read start) |
|---|---|
| JE3692-4 | 0.66827 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
| Junction allow suboptimal matches | FALSE |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 20:14:08 01 Feb 2024 | 20:16:18 01 Feb 2024 | 2 minutes 10 seconds |
| Read alignment to reference genome | 20:16:18 01 Feb 2024 | 20:27:14 01 Feb 2024 | 10 minutes 56 seconds |
| Preprocessing alignments for candidate junction identification | 20:27:14 01 Feb 2024 | 20:28:26 01 Feb 2024 | 1 minute 12 seconds |
| Preliminary analysis of coverage distribution | 20:28:26 01 Feb 2024 | 20:30:10 01 Feb 2024 | 1 minute 44 seconds |
| Identifying junction candidates | 20:30:10 01 Feb 2024 | 20:30:30 01 Feb 2024 | 20 seconds |
| Re-alignment to junction candidates | 20:30:30 01 Feb 2024 | 20:34:04 01 Feb 2024 | 3 minutes 34 seconds |
| Resolving best read alignments | 20:34:04 01 Feb 2024 | 20:35:45 01 Feb 2024 | 1 minute 41 seconds |
| Creating BAM files | 20:35:45 01 Feb 2024 | 20:37:17 01 Feb 2024 | 1 minute 32 seconds |
| Tabulating error counts | 20:37:17 01 Feb 2024 | 20:38:14 01 Feb 2024 | 57 seconds |
| Re-calibrating base error rates | 20:38:14 01 Feb 2024 | 20:38:15 01 Feb 2024 | 1 second |
| Examining read alignment evidence | 20:38:15 01 Feb 2024 | 20:49:00 01 Feb 2024 | 10 minutes 45 seconds |
| Polymorphism statistics | 20:49:00 01 Feb 2024 | 20:49:01 01 Feb 2024 | 1 second |
| Output | 20:49:01 01 Feb 2024 | 20:49:25 01 Feb 2024 | 24 seconds |
| Total | 35 minutes 17 seconds | ||
