breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 46 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*JE3692‑4699,7220AG51.5% 13.0 / 101.4 66intergenic (+115/‑129)PP_t06/PP_23SDtRNA‑Ala/23S ribosomal RNA
*JE3692‑41,327,1210CT47.3% 99.6 / 235.7 146intergenic (+97/‑196)PP_16SE/PP_23SE16S ribosomal RNA/23S ribosomal RNA
*JE3692‑44,360,8490AG45.3% 78.5 / 212.0 137intergenic (‑70/+404)PP_3820/PP_3821Group II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase
*JE3692‑44,479,7900AG36.1% 146.9 / 29.0 83intergenic (+27/‑110)PP_3958/PP_3959Na(+)/H(+) antiporter NhaA 2/Voltage‑gated chloride channel family protein
*JE3692‑4195,6340CT36.1% 314.1 / 217.0 192T380T (ACC→ACTPP_0168putative surface adhesion protein
*JE3692‑44,479,8070GC35.9% 142.3 / 55.2 83intergenic (+44/‑93)PP_3958/PP_3959Na(+)/H(+) antiporter NhaA 2/Voltage‑gated chloride channel family protein
*JE3692‑45,999,9700CT32.5% 154.8 / 73.6 80intergenic (+188/‑358)PP_5246/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
*JE3692‑45,999,9710AG32.5% 152.2 / 74.6 80intergenic (+189/‑357)PP_5246/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
*JE3692‑41,927,1720TC32.4% 59.5 / 13.9 37intergenic (+15/+46)PP_1720/PP_1721Alcohol dehydrogenase, zinc‑containing/putative phosphoserine phosphatase
*JE3692‑4196,3510CG32.1% 308.2 / 222.9 187T619T (ACC→ACGPP_0168putative surface adhesion protein
*JE3692‑45,999,9870A.32.0% 194.9 / 104.5 75intergenic (+205/‑341)PP_5246/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
*JE3692‑45,999,9810TC31.6% 155.1 / 58.3 76intergenic (+199/‑347)PP_5246/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
*JE3692‑41,904,6320AC31.2% 152.4 / 20.7 77intergenic (+70/‑450)PP_t18/PP_1703tRNA‑Asp/assimilatory nitrate reductase/sulfite reductase
*JE3692‑45,999,9650CA30.6% 165.2 / 70.2 72intergenic (+183/‑363)PP_5246/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
*JE3692‑43,636,9540GC30.0% 67.0 / 25.6 56G226G (GGC→GGGPP_3195conserved protein of unknown function
*JE3692‑4196,3900TC28.2% 312.8 / 142.0 164D632D (GAT→GACPP_0168putative surface adhesion protein
*JE3692‑42,982,6130CA26.7% 166.1 / 31.1 75intergenic (+101/+48)PP_2603/PP_2604conserved protein of unknown function/major facilitator family transporter
*JE3692‑44,479,7970CA26.5% 194.6 / 20.9 83intergenic (+34/‑103)PP_3958/PP_3959Na(+)/H(+) antiporter NhaA 2/Voltage‑gated chloride channel family protein
*JE3692‑4884,0070CT26.1% 178.1 / 24.3 100intergenic (+245/‑83)PP_0766/PP_0767conserved exported protein of unknown function/Transcriptional regulator, LuxR family
*JE3692‑42,750,7940AG26.0% 188.5 / 14.3 77intergenic (+5/+30)PP_2399/PP_2400conserved protein of unknown function/conserved protein of unknown function

Marginal new junction evidence (lowest skew 10 of 301 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? JE3692‑4 = 55666840 (0.000)52 (0.460)
+GCC
40/186 3.1 100% intergenic (‑84/+87) PP_4887/PP_4888 conserved protein of unknown function/putative methyl‑accepting chemotaxis transducer
?JE3692‑4 5566685 = 0 (0.000)intergenic (‑85/+86) PP_4887/PP_4888 conserved protein of unknown function/putative methyl‑accepting chemotaxis transducer
* ? JE3692‑4 = 49927291 (0.010)52 (0.460)
+GGC
39/186 3.2 98.2% intergenic (‑24/+44) PP_4387/PP_4388 conserved exported protein of unknown function/Flagellar hook protein FlgE
?JE3692‑4 4992730 = 1 (0.010)intergenic (‑25/+43) PP_4387/PP_4388 conserved exported protein of unknown function/Flagellar hook protein FlgE
* ? JE3692‑4 = 16449990 (0.000)41 (0.350) 34/190 4.2 100% intergenic (‑258/‑188) PP_1443/araE (Ppu opt) DNA‑binding, ATP‑dependent protease/–
?JE3692‑4 = 1652985 0 (0.000)intergenic (+174/+34) nahF (Ppu opt)/PP_1445 –/carbohydrate‑selective porin
* ? JE3692‑4 = 611136NA (NA)12 (0.110) 12/186 10.5 NA noncoding (237/1314 nt) mobile_element repeat region
?JE3692‑4 = 611169 NA (NA)noncoding (270/1314 nt) mobile_element repeat region
* ? JE3692‑4 171653 =NA (NA)9 (0.080) 9/188 12.0 NA noncoding (265/1522 nt) PP_16SA 16S ribosomal RNA
?JE3692‑4 171682 = NA (NA)noncoding (294/1522 nt) PP_16SA 16S ribosomal RNA
* ? JE3692‑4 2092023 =NA (NA)9 (0.080) 9/190 12.1 NA noncoding (628/1445 nt) mobile_element repeat region
?JE3692‑4 2092043 = NA (NA)noncoding (648/1445 nt) mobile_element repeat region
* ? JE3692‑4 = 178786NA (NA)10 (0.090) 8/182 12.3 NA noncoding (154/2902 nt) PP_23SB 23S ribosomal RNA
?JE3692‑4 = 178803 NA (NA)noncoding (171/2902 nt) PP_23SB 23S ribosomal RNA
* ? JE3692‑4 = 3012136107 (0.920)8 (0.070) 8/188 12.6 6.6% coding (3085/3621 nt) PP_2627 conserved membrane protein of unknown function
?JE3692‑4 = 3012162 121 (1.060)coding (3111/3621 nt) PP_2627 conserved membrane protein of unknown function
* ? JE3692‑4 = 2556265NA (NA)8 (0.070) 7/180 12.8 NA intergenic (+160/‑84) PP_t36/PP_23SF tRNA‑Ala/23S ribosomal RNA
?JE3692‑4 = 2556295 NA (NA)intergenic (+190/‑54) PP_t36/PP_23SF tRNA‑Ala/23S ribosomal RNA
* ? JE3692‑4 = 175674NA (NA)8 (0.070) 7/184 13.0 NA noncoding (2472/2902 nt) PP_23SA 23S ribosomal RNA
?JE3692‑4 = 175695 NA (NA)noncoding (2493/2902 nt) PP_23SA 23S ribosomal RNA