Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154514,808,1800A.65.7% 12.4 / 82.5 43intergenic (‑289/‑266)PP_4234/dsbD‑IIAldehyde oxidase and xanthine dehydrogenase/thiol/disulfide interchange protein 2
Reads supporting (aligned to +/- strand):  ref base A (8/0);  major base . (5/18);  minor base C (0/12);  total (13/30)
Fisher's exact test for biased strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.29e-02
Rejected as consensus: Frequency below/above cutoff threshold.

GGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCC  >  AE015451/4808062‑4808303
                                                                                                                      |                                                                                                                           
ggTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGgcgct                                                                                                                       <  2:5934068/125‑2 (MQ=255)
ggTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGgcgct                                                                                                                       <  1:5934069/125‑2 (MQ=255)
 gTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGgcgctt                                                                                                                      <  1:5934070/125‑3 (MQ=255)
 gTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGgcgctt                                                                                                                      <  2:5934071/125‑3 (MQ=255)
    ggTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGgcgcttcg                                                                                                                    <  2:5934073/124‑5 (MQ=255)
    ggTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGgcgcttcg                                                                                                                    <  2:5934072/124‑5 (MQ=255)
    ggTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGgcgctcga                                                                                                                    <  2:5934077/124‑5 (MQ=255)
    ggTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGgcgctcga                                                                                                                    <  2:5934075/124‑5 (MQ=255)
     gtgtTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCttcgcg                                                                                                                  <  1:5934078/125‑1 (MQ=255)
     gtgtTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCttcgcg                                                                                                                  <  1:5934079/125‑1 (MQ=255)
     gtgtTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCttcgcg                                                                                                                  <  1:5934080/125‑1 (MQ=255)
        tttGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCgcgg                                                                                                                 <  1:5934083/123‑1 (MQ=255)
                                 ggAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCaaa                                                                        <  2:5934107/125‑1 (MQ=17)
                                  gAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAac                                                                       >  1:5934230/1‑125 (MQ=17)
                                  gAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAac                                                                       <  1:5934109/125‑1 (MQ=17)
                                    ggTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAaca                                                                      <  2:5934113/124‑1 (MQ=17)
                                    ggTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAaca                                                                      <  2:5934112/124‑1 (MQ=17)
                                       gtTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCt                                                                   <  2:5934117/124‑1 (MQ=18)
                                       gtTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTc                                                                  >  1:5934237/1‑125 (MQ=17)
                                       gtTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGACCGAGCGCCGCCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCt                                                                   <  1:5934116/124‑1 (MQ=17)
                                              gggggATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTc                                                           <  1:5934118/125‑1 (MQ=17)
                                                 ggATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCaa                                                        <  2:5934119/125‑1 (MQ=17)
                                                  gATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAAc                                                       <  1:5934120/125‑1 (MQ=18)
                                                  gATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAAc                                                       <  1:5934121/125‑1 (MQ=18)
                                                  gATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAAc                                                       >  1:5934184/1‑125 (MQ=17)
                                                    ttttCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGc                                                     <  2:5934122/125‑1 (MQ=18)
                                                     tttCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGcc                                                    <  2:5934123/125‑1 (MQ=18)
                                                        cAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTc                                                 <  2:5934124/125‑1 (MQ=18)
                                                        cAGCGCCTGGGAGGTGTGCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCaa                                                        <  1:5934125/118‑1 (MQ=12)
                                                         aGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGc                                                     <  1:5934126/120‑1 (MQ=17)
                                                          gcgcCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTcc                                                >  2:5934130/1‑124 (MQ=18)
                                                          gcgcCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCa                                               <  1:5934128/125‑1 (MQ=18)
                                                            gcCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACt                                             <  1:5934130/125‑1 (MQ=18)
                                                            gcCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCACCGCCGTCCAc                                              <  2:5934129/124‑1 (MQ=17)
                                                             ccTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACt                                             >  1:5934299/1‑124 (MQ=17)
                                                                                                            tttacccgcGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAAc           >  1:5934268/5‑125 (MQ=255)
                                                                                                              tccccgcGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCGAAAATCCTCAGGCAAATACCTCAAAATTCCAACGCCATCCACTCAAAATTCCAAc                                 >  2:5934306/3‑101 (MQ=37)
                                                                                                                tccgcGAAGCGCCGCGCGGGCGGGGCTCGATCTCACAGGCGCTGATAAt                                                                                   >  1:5934242/2‑49 (MQ=17)
                                                                                                                 ccgcGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAAcgccgc      >  1:5934260/1‑125 (MQ=255)
                                                                                                                  cgcgAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACgccgcc     >  2:5934279/1‑125 (MQ=255)
                                                                                                                   gcgAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACgccgcc     >  1:5934226/1‑124 (MQ=255)
                                                                                                                    cgAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTc   >  2:5934288/1‑125 (MQ=255)
                                                                                                                     gAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTcc  >  2:5934181/1‑125 (MQ=255)
                                                                                                                      aaGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCaa            >  2:5934236/1‑114 (MQ=255)
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GGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCC  >  AE015451/4808062‑4808303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑