Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451 4,345,003 A→G E193E (GAA→GAG)  PP_3818 → Periplamic phosphate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154514,345,0030AG100.0% 303.4 / NA 306E193E (GAA→GAG) PP_3818Periplamic phosphate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (173/133);  total (173/133)

Only 100 of 306 total aligned reads displayed.

ACATTTTCTCTGGGCAGATCCAGCGCTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAACCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGTTTCAGCAGCCTGGCCGCCGTACACGGGG  >  AE015451/4344882‑4345124
                                                                                                                         |                                                                                                                         
  aTTTTCTCTGGGCAGATCCAGCGCTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCt                                                                                                                        <  1:5362040/123‑1 (MQ=255)
  aTTTTCTCTGGGCAGATCCAGCGCTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTca                                                                                                                      >  1:5362335/1‑125 (MQ=255)
     ttCTCTGGGCAGATCCAGCGCTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTcagca                                                                                                                   >  2:5362438/1‑125 (MQ=255)
     ttCTCTGGGCAGATCCAGCGCTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTcagca                                                                                                                   <  2:5362044/125‑1 (MQ=255)
      tctcTGGGCAGATCCAGCGCTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTcagcag                                                                                                                  >  1:5362380/1‑125 (MQ=255)
       ctctGGGCAGATCCAGCGCTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAgg                                                                                                                 >  1:5362424/1‑125 (MQ=255)
        tctGGGCAGATCCTGCGCTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAggg                                                                                                                <  1:5362048/125‑1 (MQ=255)
        tctGGGCAGATCCAGCGCTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTcagca                                                                                                                   >  2:5362498/1‑122 (MQ=255)
        tctGGGCAGATCCAGCGCTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAgg                                                                                                                 <  2:5362049/124‑1 (MQ=255)
          tGGGCAGATCCAGCGCTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCg                                                                                                              >  2:5362334/1‑125 (MQ=255)
          tGGGCAGATCCAGCGCTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCg                                                                                                              >  2:5362269/1‑125 (MQ=255)
            ggCAGATCCAGCGCTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCg                                                                                                              >  2:5362426/1‑123 (MQ=255)
             gCAGATCCAGCGCTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGc                                                                                                           <  2:5362052/125‑1 (MQ=255)
                 aTCCAGCGCTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTc                                                                                                       >  1:5362272/1‑125 (MQ=255)
                  tCCAGCGCTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCg                                                                                                      <  2:5362057/125‑1 (MQ=255)
                   ccAGCGCTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCgg                                                                                                     <  1:5362058/125‑1 (MQ=255)
                        gcTGGGAACAACTGGGCATACCTGGTGGGGCCATTCACCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGGGAGCTTTCGGc                                                                                                    <  2:5362063/121‑1 (MQ=255)
                         cTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCt                                                                                                          >  2:5362610/1‑114 (MQ=255)
                         cTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCgg                                                                                                     >  2:5362236/1‑119 (MQ=255)
                         cTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAagg                                                                                               <  1:5362066/125‑1 (MQ=255)
                         cTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCCAGc                                                                                                           >  1:5362478/1‑113 (MQ=255)
                          tGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAagg                                                                                               >  1:5362437/1‑124 (MQ=255)
                            ggAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCaa                                                                                                 >  2:5362671/1‑120 (MQ=255)
                             gAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAaggcgag                                                                                           >  1:5362370/1‑125 (MQ=255)
                              aacaacTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAaggcgagg                                                                                          <  2:5362070/125‑1 (MQ=255)
                              aacaacTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAaggcgagg                                                                                          >  1:5362505/1‑125 (MQ=255)
                               acaacTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAaggcgaggc                                                                                         <  1:5362071/125‑1 (MQ=255)
                                  acTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGTCGt                                                                                       >  2:5362350/1‑124 (MQ=255)
                                  acTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGtt                                                                                      <  2:5362075/125‑1 (MQ=255)
                                   cTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCt                                                                                                          >  2:5362577/1‑104 (MQ=255)
                                   cTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGttt                                                                                     <  2:5362079/125‑1 (MQ=255)
                                   cTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGttt                                                                                     <  1:5362081/125‑1 (MQ=255)
                                    tGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACAGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTc                                                                                    <  2:5362085/125‑1 (MQ=255)
                                      ggCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGa                                                                                  <  1:5362086/125‑1 (MQ=255)
                                       gCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAg                                                                                 >  1:5362222/1‑125 (MQ=255)
                                        cATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGt                                                                                >  2:5362563/1‑125 (MQ=255)
                                          tACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTcc                                                                              >  1:5362870/1‑125 (MQ=255)
                                          tACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTcc                                                                              >  1:5362815/1‑125 (MQ=255)
                                          tACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTcc                                                                              <  1:5362089/125‑1 (MQ=255)
                                              tggtggGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTg                                                                          >  2:5362400/1‑125 (MQ=255)
                                               ggtggGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGa                                                                         <  1:5362094/125‑1 (MQ=255)
                                                gtggGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGAc                                                                        >  1:5362568/1‑125 (MQ=255)
                                                gtggGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGAc                                                                        <  1:5362097/125‑1 (MQ=255)
                                                 tggGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACg                                                                       >  2:5362309/1‑125 (MQ=255)
                                                  ggggCCATTCAGCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAaggcgagg                                                                                          <  2:5362098/105‑1 (MQ=255)
                                                    ggCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAAt                                                                    >  2:5362202/1‑125 (MQ=255)
                                                    ggCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAAt                                                                    <  1:5362103/125‑1 (MQ=255)
                                                     gCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAAtt                                                                   <  2:5362105/125‑1 (MQ=255)
                                                       cATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTgg                                                                 >  2:5362467/1‑125 (MQ=255)
                                                       cATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTgg                                                                 >  1:5362433/1‑125 (MQ=255)
                                                        aTTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTggc                                                                >  1:5362443/1‑125 (MQ=255)
                                                          tCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTggcgg                                                              <  2:5362109/125‑1 (MQ=255)
                                                                 tATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCAcgcg                                                        >  2:5362447/1‑124 (MQ=255)
                                                                  aTGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTg                                                      <  1:5362112/125‑1 (MQ=255)
                                                                   tGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTgg                                                     <  1:5362116/125‑1 (MQ=255)
                                                                   tGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTg                                                      <  2:5362117/124‑1 (MQ=255)
                                                                       cGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTac                                                 <  1:5362120/125‑1 (MQ=255)
                                                                         tGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTac                                                 >  1:5362326/1‑123 (MQ=255)
                                                                          gacgacCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTaccga                                              <  1:5362125/125‑1 (MQ=255)
                                                                           acgacCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCAcgcg                                                        >  1:5362240/1‑114 (MQ=255)
                                                                           acgacCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTaccgac                                             <  2:5362126/125‑1 (MQ=255)
                                                                              acCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGc                                          >  1:5362393/1‑125 (MQ=255)
                                                                               ccGTTCCGGCACTTGCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTaccgac                                             <  2:5362131/121‑1 (MQ=255)
                                                                                cGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCa                                        <  1:5362134/125‑1 (MQ=255)
                                                                                  ttCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAgg                                      >  2:5362492/1‑125 (MQ=255)
                                                                                     cGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCAAGGCCa                                   <  1:5362138/125‑1 (MQ=255)
                                                                                      ggCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAgg                                      <  1:5362142/121‑1 (MQ=255)
                                                                                       gCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCTGCACGCGTGGGTACCGACCGCCAGGCCATc                                 >  2:5362597/1‑125 (MQ=255)
                                                                                       gCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATc                                 >  1:5362661/1‑125 (MQ=255)
                                                                                       gCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATc                                 <  2:5362143/125‑1 (MQ=255)
                                                                                       gCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATc                                 >  2:5362376/1‑125 (MQ=255)
                                                                                         aatttCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGCGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCg                                >  2:5362411/3‑124 (MQ=255)
                                                                                         aCTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCgg                               >  1:5362348/1‑125 (MQ=255)
                                                                                         aCTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCgg                               >  2:5362487/1‑125 (MQ=255)
                                                                                         aCTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCg                                <  2:5362147/124‑1 (MQ=255)
                                                                                          cTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCa                                   >  1:5362626/1‑120 (MQ=255)
                                                                                          cTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGt                              <  1:5362148/125‑1 (MQ=255)
                                                                                          cTTTCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGGTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCAt                                  >  1:5362522/1‑121 (MQ=255)
                                                                                           tttCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCAt                                  >  1:5362719/1‑120 (MQ=255)
                                                                                             tCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGTCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGTTTc                           >  1:5362470/1‑125 (MQ=255)
                                                                                             tCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGTTTc                           >  1:5362461/1‑125 (MQ=255)
                                                                                             tCGAGACCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGACTCCAGTGACGAATTGGCGGCAcgc                                                         >  2:5362550/1‑95 (MQ=255)
                                                                                               gagaCCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGTTTcag                         <  2:5362152/125‑1 (MQ=255)
                                                                                               gagaCCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGTTTcag                         >  1:5362296/1‑125 (MQ=255)
                                                                                                agaCCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCCGGCCATCGGTTTcagc                        >  2:5362274/1‑125 (MQ=255)
                                                                                                 gaCCTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGACACGCGTGGGTACCGACCGCCAGGCCATCGGTTTcagca                       >  1:5362538/1‑125 (MQ=255)
                                                                                                   ccTTCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGTTTCagcagc                     >  1:5362402/1‑125 (MQ=255)
                                                                                                     ttCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGTTTCAGCAGCCt                   <  2:5362156/125‑1 (MQ=255)
                                                                                                     ttCAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGTTTCAGCAGCCt                   >  1:5362552/1‑125 (MQ=255)
                                                                                                     tacAAGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGTTTCAGCAGcc                    >  2:5362507/3‑124 (MQ=255)
                                                                                                        aaGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGTTTCAGCAGCCTGgc                <  1:5362157/125‑1 (MQ=255)
                                                                                                         aGGCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGTTTCAGCAGCCTGgcc               >  1:5362587/1‑125 (MQ=255)
                                                                                                          ggCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGTTTca                          <  2:5362162/113‑1 (MQ=255)
                                                                                                          ggCACTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGTTTCAGCAGCCTGgccg              <  1:5362163/125‑1 (MQ=255)
                                                                                                             aCTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGTTTCAGCAGCCTGGccgccg           <  2:5362166/125‑1 (MQ=255)
                                                                                                             aCTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGTTTCAGCAGCCTGGccgccg           >  1:5362513/1‑125 (MQ=255)
                                                                                                             aCTGGTGCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGGACCCACCGCCAGGCCATCGGTTTcagcag                      >  2:5362804/1‑114 (MQ=255)
                                                                                                                   gCTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGTTTCAGCAGCCTGGCCGCCGTACACg     <  1:5362170/125‑1 (MQ=255)
                                                                                                                    cTGGAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGTTTCAGCAGCCTGGCCGCCGTACACgg    <  2:5362172/125‑1 (MQ=255)
                                                                                                                      ggAGCCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGTTTCAGCAGCCTGGCCGCCGTACACgggg  >  2:5362520/1‑125 (MQ=255)
                                                                                                                         |                                                                                                                         
ACATTTTCTCTGGGCAGATCCAGCGCTGGGAACAACTGGGCATACCTGGTGGGGCCATTCATCTGTATGCCCGTGACGACCGTTCCGGCACTTTCGAGACCTTCAAGGCACTGGTGCTGGAACCTCAGCAGGGCGAGCTTTCGGCCAAGGCGAGGCGTTTCGAGTCCAGTGACGAATTGGCGGCACGCGTGGGTACCGACCGCCAGGCCATCGGTTTCAGCAGCCTGGCCGCCGTACACGGGG  >  AE015451/4344882‑4345124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑