Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451 5,740,555 C→T S175N (AGC→AAC)  PP_5037 ← lipocalin family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154515,740,5550CT99.7% 980.0 / NA 343S175N (AGC→AAC) PP_5037lipocalin family lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (175/167);  minor base A (0/1);  total (175/168)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 343 total aligned reads displayed.

GCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCCGACGATGGCCGT  >  AE015451/5740436‑5740676
                                                                                                                       |                                                                                                                         
gCGTACCGGGGTTGCGTTGCAGCCCTTGGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTgg                                                                                                                        <  2:7228386/123‑1 (MQ=255)
    aCCGGGGTTGCGTGGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTc                                                                                                                    <  2:7228390/123‑1 (MQ=255)
      cGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCAt                                                                                                                  <  1:7228391/123‑1 (MQ=255)
        gggTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATAccc                                                                                                              <  2:7228395/125‑1 (MQ=255)
          gttgcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATAcccc                                                                                                             >  2:7228783/1‑124 (MQ=255)
          gttgcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATAcccc                                                                                                             >  2:7228859/1‑124 (MQ=255)
          gttgcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATAcccc                                                                                                             >  2:7228932/1‑124 (MQ=255)
           ttgcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCctg                                                                                                           >  1:7228745/1‑125 (MQ=255)
             gcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCctgct                                                                                                         <  1:7228399/125‑1 (MQ=255)
              cgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATAcccc                                                                                                             <  2:7228403/120‑1 (MQ=255)
               gttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCtgctgc                                                                                                       >  2:7228964/1‑125 (MQ=255)
                ttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCtgctgc                                                                                                       <  1:7228406/124‑1 (MQ=255)
                ttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGcc                                                                                                      >  1:7228943/1‑125 (MQ=255)
                    aGCCCTTCGCCTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCgcgc                                                                                                  >  2:7228611/1‑125 (MQ=255)
                       ccTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCt                                                                                               <  2:7228412/125‑1 (MQ=255)
                         ttCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTg                                                                                              <  1:7228416/124‑1 (MQ=255)
                              gTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagc                                                                                        <  1:7228420/125‑1 (MQ=255)
                               tGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagca                                                                                       >  2:7228688/1‑125 (MQ=255)
                                  aaGCCCGCGAAGGGTTGCAACGCAACCCCAAAAATCTCAGTGCATTTTTACTATTTGCTGATCGGACGCCAGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGt                                                                                    <  1:7228425/125‑1 (MQ=255)
                                   aGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGtt                                                                                   >  1:7228961/1‑125 (MQ=255)
                                   aGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGtt                                                                                   <  2:7228428/125‑1 (MQ=255)
                                   aGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGt                                                                                    >  1:7229107/1‑124 (MQ=255)
                                      ccGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtct                                                                                 <  1:7228430/124‑1 (MQ=255)
                                      ccGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCACATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGAc                                                                                           >  2:7228792/1‑114 (MQ=255)
                                       cgcgAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa                                                                               >  2:7229001/1‑125 (MQ=255)
                                        gcgAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa                                                                               <  2:7228434/124‑1 (MQ=255)
                                         cgAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACg                                                                             >  1:7228896/1‑125 (MQ=255)
                                         cgAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACg                                                                             >  1:7228776/1‑125 (MQ=255)
                                          gAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtctc                                                                                <  1:7228439/121‑1 (MQ=255)
                                          gAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAc                                                                              >  2:7228979/1‑123 (MQ=255)
                                           aaGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa                                                                               <  2:7228443/121‑1 (MQ=255)
                                           aaGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCt                                                                           <  1:7228444/125‑1 (MQ=255)
                                            aGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTc                                                                          >  2:7228942/1‑125 (MQ=255)
                                               gTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCa                                                                       <  1:7228448/125‑1 (MQ=255)
                                                 tGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtctc                                                                                >  2:7228710/1‑114 (MQ=255)
                                                   caacgcaacCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGcc                                                                   >  1:7228793/1‑125 (MQ=255)
                                                   caacgcaacCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc                                                                    <  1:7228454/124‑1 (MQ=255)
                                                    aacgcaacCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCg                                                                  >  1:7229072/1‑125 (MQ=255)
                                                      cgcaacCCCAAAAAGCTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTccc                                                                        <  2:7228455/117‑1 (MQ=255)
                                                      cgcaacCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCATGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGc                                                                >  2:7228882/1‑125 (MQ=255)
                                                           ccccAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAgtgt                                                           >  2:7228835/1‑125 (MQ=255)
                                                           ccccAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACACCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAAGCTccc                                                                        >  2:7228926/1‑112 (MQ=255)
                                                            cccAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagc                                                                                        >  2:7228850/1‑95 (MQ=255)
                                                             ccAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATAACCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCg                                                         <  2:7228462/125‑1 (MQ=255)
                                                              cAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCgg                                                        <  1:7228464/125‑1 (MQ=255)
                                                              cAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCgg                                                        >  2:7228722/1‑125 (MQ=255)
                                                               aaaaaaCTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATa                                                                                                                 >  2:7228815/1‑67 (MQ=255)
                                                               aaaaaaCTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTc                                                          <  2:7228470/122‑1 (MQ=255)
                                                                  aaaCTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGgtgt                                                     >  1:7228974/1‑124 (MQ=255)
                                                                  aaaCTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGtgtg                                                    <  1:7228475/125‑1 (MQ=255)
                                                                    aCTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTgcg                                                  <  2:7228476/125‑1 (MQ=255)
                                                                      tCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGcgcg                                                >  2:7228771/1‑125 (MQ=255)
                                                                      tCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGCTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTgcgc                                                 >  2:7228753/1‑124 (MQ=255)
                                                                       cAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc                                                                         <  2:7228482/99‑1 (MQ=255)
                                                                       cAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGcgcg                                                <  1:7228483/124‑1 (MQ=255)
                                                                           gCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGAGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGa                                           >  2:7228677/1‑125 (MQ=255)
                                                                            cATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTgcgc                                                 >  2:7228869/1‑118 (MQ=255)
                                                                             aTTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAAt                                         >  1:7228868/1‑125 (MQ=255)
                                                                             aTTTTTACTATTGGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGaa                                          <  1:7228489/124‑1 (MQ=255)
                                                                              tttttACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAAt                                         <  2:7228491/124‑1 (MQ=255)
                                                                                  tACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCaaa                                    >  1:7228733/1‑125 (MQ=255)
                                                                                  tACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCAGCGTGCGCGACAGAATCGaaa                                    <  1:7228493/125‑1 (MQ=255)
                                                                                   aCTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAg                                   <  1:7228496/125‑1 (MQ=255)
                                                                                     tATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAgg                                  >  1:7228667/1‑124 (MQ=255)
                                                                                     tATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGt                                 <  2:7228500/125‑1 (MQ=255)
                                                                                      aTTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAgg                                  <  2:7228505/123‑1 (MQ=255)
                                                                                       tttGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTa                                <  1:7228509/124‑1 (MQ=255)
                                                                                       tttGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTAt                               <  2:7228510/125‑1 (MQ=255)
                                                                                       tttGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTAt                               >  1:7228736/1‑125 (MQ=255)
                                                                                        ttGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTAtt                              <  1:7228513/125‑1 (MQ=255)
                                                                                          gCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTAtt                              <  2:7228517/123‑1 (MQ=255)
                                                                                          gCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTg                            >  1:7228998/1‑125 (MQ=255)
                                                                                           cTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCACGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTAttt                             >  1:7228699/1‑123 (MQ=255)
                                                                                            tGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTAttt                             <  2:7228521/122‑1 (MQ=255)
                                                                                            tGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCg                          >  2:7229051/1‑125 (MQ=255)
                                                                                            tGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCg                          >  2:7229085/1‑125 (MQ=255)
                                                                                             gATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCgg                         >  1:7228819/1‑125 (MQ=255)
                                                                                              aTCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGc                           >  1:7228911/1‑122 (MQ=255)
                                                                                               tCGGACGCCCGCCGGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCgg                         <  1:7228525/123‑1 (MQ=255)
                                                                                               tCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTc                       >  1:7229195/1‑125 (MQ=255)
                                                                                                cGGACGCCCGGCAGATCAAGCGGTTGGTGTCATACCCCTGCGGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCgg                         <  1:7228530/122‑1 (MQ=255)
                                                                                                cGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCgg                                                        <  2:7228531/91‑1 (MQ=255)
                                                                                                  gACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCgg                    >  2:7228906/1‑125 (MQ=255)
                                                                                                  gACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCgg                    <  2:7228535/125‑1 (MQ=255)
                                                                                                    cgcccgccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGc                  >  1:7228881/1‑125 (MQ=255)
                                                                                                    cgcccgccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGc                  <  2:7228538/125‑1 (MQ=255)
                                                                                                      cccgccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTg                <  1:7228542/125‑1 (MQ=255)
                                                                                                       ccgccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGc               >  2:7228840/1‑125 (MQ=255)
                                                                                                        cgccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGcc              <  2:7228546/125‑1 (MQ=255)
                                                                                                         gccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTg                            <  1:7228548/110‑1 (MQ=255)
                                                                                                         gccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCcg             >  1:7228982/1‑125 (MQ=255)
                                                                                                         gccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCcg             >  1:7228839/1‑125 (MQ=255)
                                                                                                          ccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCCGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTGTTTGCGGTCCGGGCTGCcga            >  1:7228936/1‑125 (MQ=255)
                                                                                                          ccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCcg             >  1:7229031/1‑124 (MQ=255)
                                                                                                          ccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCcg             >  1:7229289/1‑124 (MQ=255)
                                                                                                           cAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCcgac           <  1:7228551/125‑1 (MQ=255)
                                                                                                               tCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCCGACGATg       <  2:7228555/125‑1 (MQ=255)
                                                                                                                 aaGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCCGACGATGGc     <  1:7228557/125‑1 (MQ=255)
                                                                                                                  aGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCCGACGATGGcc    >  1:7228917/1‑125 (MQ=255)
                                                                                                                    cGGTTGGTGTCATACCCCTGTTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCCGACGATGGCCGt  <  1:7228561/125‑1 (MQ=255)
                                                                                                                       |                                                                                                                         
GCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCCGACGATGGCCGT  >  AE015451/5740436‑5740676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑