BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	1,499,497	1	.	C	69.2%	117.0 / 208.6	172	intergenic (+74/‑90)	trpS/zapE	Tryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand): ref base . (12/41); new base C (61/58); total (73/99)
Fisher's exact test for biased strand distribution p-value = 4.46e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 182 total aligned reads displayed.

GATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑T‑CGGGCACG‑C‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGC  >  AE015451/1499373‑1499612

gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt‑t                                                                                                                          <  1:1390974/125‑1 (MQ=255)

 aTGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt‑t                                                                                                                          >  1:1391141/1‑124 (MQ=255)

  tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt‑tcg                                                                                                                        <  2:1390981/125‑3 (MQ=255)

  tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt‑t                                                                                                                          >  2:1391174/1‑123 (MQ=255)

   gCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt‑tcg                                                                                                                        <  1:1390982/124‑3 (MQ=255)

    cGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt‑tcgcg                                                                                                                      <  2:1390983/125‑5 (MQ=255)

    cGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCC‑CGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt‑tcgcgg                                                                                                                     >  1:1391011/1‑120 (MQ=37)

          ttCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCAc                                                                                                                <  2:1390991/124‑1 (MQ=25)

           tCAGAGCCTGAACGAAGCCTTGGCACCAAAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCAcg‑c                                                                                                             <  2:1390993/125‑1 (MQ=25)

            cAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCAcg‑c‑c                                                                                                           <  2:1390995/125‑1 (MQ=25)

                gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCAcg‑c‑ccgct                                                                                                       <  2:1390997/125‑5 (MQ=21)

                gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCAcg‑c‑ccg                                                                                                         >  1:1391312/1‑121 (MQ=21)

                 ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCAcg‑c‑ccgctc                                                                                                      <  1:1390998/125‑6 (MQ=21)

                   tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTccc                                                                                                    >  1:1391208/1‑125 (MQ=18)

                    gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTccc                                                                                                    >  1:1391147/1‑124 (MQ=18)

                     aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCcac                                                                                                  <  2:1391000/125‑1 (MQ=18)

                         aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAg                                                                                                >  1:1391072/1‑123 (MQ=18)

                         aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGAc                                                                                              <  2:1391002/125‑1 (MQ=18)

                           gCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAg                                                                                                >  2:1391308/1‑121 (MQ=18)

                             cTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTg                                                                                            >  1:1391520/1‑123 (MQ=18)

                             cTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTGAt                                                                                          >  1:1391160/1‑125 (MQ=18)

                             cTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTGAt                                                                                          <  1:1391003/125‑1 (MQ=18)

                                  caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCa                                                                                     <  1:1391006/125‑1 (MQ=18)

                                    ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCAtt                                                                                   >  1:1391185/1‑125 (MQ=18)

                                    ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCAtt                                                                                   <  2:1391008/125‑1 (MQ=18)

                                    ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCAtt                                                                                   <  1:1391010/125‑1 (MQ=18)

                                          cagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCC‑CGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCgg                                                                             <  2:1391011/124‑1 (MQ=18)

                                            ggACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCggg                                                                            <  1:1391014/124‑1 (MQ=18)

                                            ggACTGAGGCTGCCAAACGGCGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTAC‑G‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCCTTAAGCGGGc                                                                           >  2:1391178/1‑125 (MQ=17)

                                             gACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGcc                                                                          <  2:1391017/125‑1 (MQ=18)

                                             gACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATCAAGCGGGcc                                                                          <  2:1391015/125‑1 (MQ=18)

                                               cTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTa                                                                        <  1:1391020/125‑1 (MQ=18)

                                               cTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCt                                                                         <  1:1391018/124‑1 (MQ=18)

                                                 gAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAg                                                                                <  2:1391022/115‑1 (MQ=17)

                                                 gAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATc                                                                      >  1:1391292/1‑125 (MQ=18)

                                                  aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATc                                                                      >  2:1391166/1‑124 (MQ=18)

                                                  aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg                                                                     >  1:1391218/1‑125 (MQ=18)

                                                  aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg                                                                     >  1:1391351/1‑125 (MQ=18)

                                                   ggCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGc                                                                    <  1:1391023/125‑1 (MQ=18)

                                                       gCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGc                                                                    >  2:1391266/1‑121 (MQ=17)

                                                       gCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTAc                                                                 <  1:1391025/124‑1 (MQ=18)

                                                        ccAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGTCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTCAGCGGGCCTATCGCTACa                                                                >  2:1391290/1‑124 (MQ=17)

                                                        ccAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCGACAg                                                               >  2:1391054/1‑125 (MQ=17)

                                                         cAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGt                                                              <  2:1391027/125‑1 (MQ=18)

                                                         cAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGt                                                              <  1:1391028/125‑1 (MQ=18)

                                                          aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTg                                                             >  1:1391383/1‑125 (MQ=18)

                                                          aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTg                                                             >  2:1391061/1‑125 (MQ=18)

                                                           aaCGGTGGGAGCGGGCGTGCCCGCGGAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGa                                                            <  1:1391030/125‑1 (MQ=18)

                                                             cGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACg                                                          >  1:1391241/1‑125 (MQ=18)

                                                             cGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACg                                                          <  2:1391033/125‑1 (MQ=18)

                                                               gTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGc                                                        <  1:1391035/125‑1 (MQ=18)

                                                                 gggAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGcc                                                       <  2:1391037/124‑1 (MQ=18)

                                                                    aGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCg                                                     >  2:1391257/1‑123 (MQ=18)

                                                                          cGTGCGCGCGAAACAGGCGCCGCGGTGCATGGCACGGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttg                                              <  1:1391039/124‑1 (MQ=21)

                                                                           gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                            <  2:1391040/125‑1 (MQ=21)

                                                                            tGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                            >  1:1391275/1‑124 (MQ=21)

                                                                            tGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                            >  2:1391213/1‑124 (MQ=21)

                                                                             gCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGc                                          <  1:1391044/125‑1 (MQ=21)

                                                                             gCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGc                                          <  2:1391043/125‑1 (MQ=21)

                                                                              cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGcc                                         <  2:1391045/125‑1 (MQ=21)

                                                                              cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGcc                                         <  1:1391046/125‑1 (MQ=21)

                                                                                cgcgAAACAGGCGCCGCGGTGCATGGCACCGGTTTTGCCGGTGT‑T‑CGCGGGCACG‑C‑CTGCTCGCACAGACTGTTTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCg                                                     <  1:1391049/111‑1 (MQ=21)

                                                                                 gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCt                                        <  2:1391051/123‑1 (MQ=25)

                                                                                 gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCt                                        <  1:1391052/123‑1 (MQ=25)

                                                                                  cgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTGCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTc                                       >  1:1391187/1‑123 (MQ=25)

                                                                                   gAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCGCAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt                                             <  1:1391055/116‑1 (MQ=21)

                                                                                    aaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTa                                   >  1:1391409/1‑125 (MQ=25)

                                                                                    aaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTa                                   <  1:1391057/125‑1 (MQ=25)

                                                                                     aaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa                                  >  2:1391278/1‑125 (MQ=25)

                                                                                      aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CTGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGCGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAAc                                 >  1:1391297/1‑125 (MQ=25)

                                                                                      aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa                                  >  1:1391468/1‑124 (MQ=25)

                                                                                      aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAAc                                 >  1:1391250/1‑125 (MQ=25)

                                                                                       cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACg                                <  1:1391059/125‑1 (MQ=25)

                                                                                         ggCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGaa                              >  1:1391436/1‑125 (MQ=25)

                                                                                          gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGaa                              >  1:1391344/1‑124 (MQ=25)

                                                                                          gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt                             <  1:1391061/125‑1 (MQ=25)

                                                                                           cgccgcGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc                            <  1:1391064/125‑1 (MQ=25)

                                                                                              cgcgGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATg                         <  2:1391065/125‑1 (MQ=25)

                                                                                              cgcgGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATg                         >  2:1391318/1‑125 (MQ=25)

                                                                                               gcgGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa                        <  1:1391067/125‑1 (MQ=25)

                                                                                                cgGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGAc                       <  2:1391069/125‑1 (MQ=25)

                                                                                                cgGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGAc                       >  1:1391234/1‑125 (MQ=25)

                                                                                                 ggTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGAc                       >  1:1391294/1‑124 (MQ=25)

                                                                                                 ggTGCATGGCACCGGCTTTGCCGGTGT‑GCG‑CGGGCACG‑CGC‑GCTCCCAGAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGttttt                                                <  2:1391071/99‑1 (MQ=21)

                                                                                                  gTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc                     <  2:1391074/125‑1 (MQ=25)

                                                                                                  gTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc                     >  1:1391588/1‑125 (MQ=37)

                                                                                                      aTGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                  <  2:1391078/124‑1 (MQ=25)

                                                                                                      aTGGCACCGGCTTTGCCGGTGT‑T‑CGCGGTCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGGGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                  <  2:1391076/124‑1 (MQ=25)

                                                                                                       tggcaccGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                >  1:1391281/7‑125 (MQ=37)

                                                                                                         gcaccGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTccc                   >  1:1391238/5‑120 (MQ=37)

                                                                                                          caccGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa             <  2:1391082/122‑1 (MQ=37)

                                                                                                          caccGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTT‑TTGCCTCGCTAACGAATCATGACTCCCTTAGAAc            <  2:1391081/122‑1 (MQ=37)

                                                                                                            ccGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAAc            <  1:1391080/123‑1 (MQ=38)

                                                                                                              ggCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt         <  1:1391083/125‑1 (MQ=38)

                                                                                                                 tttGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt         >  2:1391270/1‑122 (MQ=38)

                                                                                                                 tttGCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt       >  2:1391329/1‑124 (MQ=38)

                                                                                                                  ttGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCa     <  1:1391086/125‑1 (MQ=39)

                                                                                                                  ttGCCGGTGT‑T‑CGCGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCa     <  2:1391087/125‑1 (MQ=39)

                                                                                                                  tggCCGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt       <  2:1391096/121‑1 (MQ=38)

                                                                                                                     ccGGTGT‑TCG‑CGGGCACGCC‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCTCTTAGAACGCTATCAAGc  >  2:1391225/1‑125 (MQ=39)

GATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑T‑CGGGCACG‑C‑C‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGC  >  AE015451/1499373‑1499612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`