Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451 5,740,555 C→T S175N (AGC→AAC)  PP_5037 ← lipocalin family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154515,740,5550CT100.0% 765.5 / NA 224S175N (AGC→AAC) PP_5037lipocalin family lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (120/104);  total (120/104)

Only 100 of 224 total aligned reads displayed.

AGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCCGACGATGGCC  >  AE015451/5740435‑5740674
                                                                                                                        |                                                                                                                       
aGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTgg                                                                                                                      >  2:4960282/1‑124 (MQ=255)
 gCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTg                                                                                                                       <  2:4960054/122‑1 (MQ=255)
 gCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGgtg                                                                                                                    >  2:4960320/1‑125 (MQ=255)
   gTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATCTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGgtgt                                                                                                                   >  2:4960328/1‑124 (MQ=255)
    tACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCa                                                                                                                 >  2:4960300/1‑125 (MQ=255)
      ccGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCTAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATa                                                                                                               <  2:4960057/125‑1 (MQ=255)
      ccGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGgtgt                                                                                                                   <  1:4960058/121‑1 (MQ=255)
      ccGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATa                                                                                                               >  1:4960369/1‑125 (MQ=255)
       cGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATAc                                                                                                              <  1:4960060/125‑1 (MQ=255)
           gttgcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTTTCATACCCct                                                                                                          <  2:4960063/125‑1 (MQ=255)
           gttgcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCct                                                                                                          >  1:4960405/1‑125 (MQ=255)
             tgcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCctgc                                                                                                        >  2:4960262/1‑125 (MQ=255)
                gttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCtgctgc                                                                                                     <  2:4960065/125‑1 (MQ=255)
                 ttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCctgctg                                                                                                      <  2:4960067/123‑1 (MQ=255)
                 ttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGcc                                                                                                    >  1:4960360/1‑125 (MQ=255)
                   gcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCcg                                                                                                   <  2:4960069/124‑1 (MQ=255)
                       cccTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGACCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCcgcg                                                                                                 >  2:4960400/1‑122 (MQ=255)
                        ccTTCGCGTGCAAGCCCGCGAGGGGTTGCAACGCACCCCCAAACAACTCAGTGCATTTTTACTATTTGCGGATCGGACGCCCGCCAGATCAAGCGGTTGgtgt                                                                                                                   <  1:4960071/103‑1 (MQ=255)
                           tCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTgg                                                                                           >  1:4960213/1‑124 (MQ=255)
                             gcgTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGAc                                                                                         >  2:4960312/1‑124 (MQ=255)
                              cgTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATATGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGAca                                                                                        >  1:4960458/1‑124 (MQ=255)
                                tGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagc                                                                                      >  1:4960448/1‑124 (MQ=255)
                                 gCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagcag                                                                                    >  1:4960158/1‑125 (MQ=255)
                                 gCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagca                                                                                     <  1:4960074/124‑1 (MQ=255)
                                  cAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAgg                                                                                   <  1:4960076/125‑1 (MQ=255)
                                    aGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGtt                                                                                 <  2:4960078/125‑1 (MQ=255)
                                        cgcgAAGGGTTGCAACGCAGCCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa                                                                             <  1:4960082/125‑1 (MQ=255)
                                        cgcgAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa                                                                             <  2:4960083/125‑1 (MQ=255)
                                          cgAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAc                                                                            >  1:4960244/1‑124 (MQ=255)
                                             aGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGa                                                                                          <  1:4960085/107‑1 (MQ=255)
                                              gggTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGcc                                                                                                    <  2:4960088/96‑1 (MQ=255)
                                              gggTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc                                                                       >  1:4960501/1‑125 (MQ=255)
                                                gTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCcg                                                                                                   <  2:4960090/95‑1 (MQ=255)
                                                   gcaacgcaacCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATAcccc                                                                                                           >  2:4960389/1‑84 (MQ=255)
                                                   gcaacgcaacCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc                                                                  <  2:4960093/125‑1 (MQ=255)
                                                   gcaacgcaacCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc                                                                  >  2:4960161/1‑125 (MQ=255)
                                                   gcaacgcaacCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc                                                                  <  2:4960095/125‑1 (MQ=255)
                                                     aacgcaacCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCg                                                                >  1:4960324/1‑125 (MQ=255)
                                                       cgcagcCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATAccc                                                                                                            <  2:4960097/79‑1 (MQ=255)
                                                          aacCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGACCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGcc                                                                 >  2:4960382/1‑119 (MQ=255)
                                                           acCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGc                                                              >  1:4960516/1‑121 (MQ=255)
                                                            ccccAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAgtgt                                                         <  1:4960100/125‑1 (MQ=255)
                                                               cAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCgg                                                      <  2:4960101/125‑1 (MQ=255)
                                                                 aaaaaCTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGgtg                                                    >  1:4960264/1‑125 (MQ=255)
                                                                  aaaaCTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGgtg                                                    >  1:4960224/1‑124 (MQ=255)
                                                                   aaaCTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGtgtg                                                  <  1:4960103/125‑1 (MQ=255)
                                                                     aCTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTgc                                                 >  2:4960349/1‑124 (MQ=255)
                                                                      cTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCGCGCTCCCAGGCCGGCAGTGTCGGTGTgcg                                                <  2:4960106/124‑1 (MQ=255)
                                                                      cTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTgcgc                                               >  2:4960479/1‑125 (MQ=255)
                                                                      cTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTgcg                                                >  2:4960227/1‑124 (MQ=255)
                                                                      cTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTgc                                                 <  1:4960108/123‑1 (MQ=255)
                                                                       tCAGTGCATTTTTAATATTTGCTGATCGGACGCCCGCCAGGTCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCATGCCGGCAGTGTCGGTGTgcg                                                >  2:4960255/1‑123 (MQ=255)
                                                                         aGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGAc                                            >  1:4960329/1‑125 (MQ=255)
                                                                         aGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGAc                                            >  2:4960304/1‑125 (MQ=255)
                                                                          gTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTccc                                                                      >  2:4960523/1‑98 (MQ=255)
                                                                          gTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTgcgc                                               >  2:4960449/1‑121 (MQ=255)
                                                                          gTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACa                                           <  2:4960111/125‑1 (MQ=255)
                                                                           tGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAg                                          >  1:4960409/1‑125 (MQ=255)
                                                                            gCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGa                                         <  1:4960113/125‑1 (MQ=255)
                                                                             cATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAg                                          <  1:4960115/123‑1 (MQ=255)
                                                                               tttttACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGccc                                                                                              <  2:4960118/69‑1 (MQ=255)
                                                                               tttttACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATc                                      >  1:4960417/1‑125 (MQ=255)
                                                                               tttttACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATc                                      >  1:4960319/1‑125 (MQ=255)
                                                                               tttttACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGCCCGGCAGTGTCGGTGTGCGCGACa                                           >  2:4960565/1‑120 (MQ=255)
                                                                                 tttACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCa                                    <  2:4960120/125‑1 (MQ=255)
                                                                                   tACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCaaa                                  >  1:4960384/1‑125 (MQ=255)
                                                                                    aCTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAg                                 >  1:4960338/1‑125 (MQ=255)
                                                                                     cTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAgg                                <  2:4960123/125‑1 (MQ=255)
                                                                                      tATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAg                                 >  2:4960418/1‑123 (MQ=255)
                                                                                      tATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGt                               >  1:4960436/1‑125 (MQ=255)
                                                                                        tttGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTAt                             >  1:4960284/1‑125 (MQ=255)
                                                                                         ttGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTa                              <  2:4960126/123‑1 (MQ=255)
                                                                                         ttGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTAtt                            <  1:4960125/125‑1 (MQ=255)
                                                                                         ttGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTAtt                            >  1:4960402/1‑125 (MQ=255)
                                                                                          tGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTAttt                           <  2:4960128/125‑1 (MQ=255)
                                                                                           gCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTg                          <  2:4960132/125‑1 (MQ=255)
                                                                                            cTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGc                         <  1:4960134/125‑1 (MQ=255)
                                                                                             tGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCg                        >  1:4960197/1‑125 (MQ=255)
                                                                                              gATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCATGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCgg                       <  1:4960136/125‑1 (MQ=255)
                                                                                              gATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCgg                       >  2:4960184/1‑125 (MQ=255)
                                                                                               aTCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCg                        >  2:4960473/1‑123 (MQ=255)
                                                                                               aTCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGt                      <  1:4960138/125‑1 (MQ=255)
                                                                                                tCGGACGCCCGCCAGATCAAGCTGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATc                                      <  1:4960140/108‑1 (MQ=255)
                                                                                                   gACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTAtt                            <  2:4960143/115‑1 (MQ=255)
                                                                                                     cgcccgccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGc                >  1:4960484/1‑125 (MQ=255)
                                                                                                     cgcccgccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGc                <  1:4960145/125‑1 (MQ=255)
                                                                                                      gcccgccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCt               >  1:4960260/1‑125 (MQ=255)
                                                                                                       cccgccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTTCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCggg                 <  2:4960148/122‑1 (MQ=255)
                                                                                                       cccgccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGc                <  1:4960149/123‑1 (MQ=255)
                                                                                                        ccgccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGc             >  2:4960535/1‑125 (MQ=255)
                                                                                                         cgccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTcc                    >  2:4960505/1‑117 (MQ=255)
                                                                                                         cgccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGcc            >  1:4960390/1‑125 (MQ=255)
                                                                                                          gccAGATCAAGCGGTTGGTGTCATATCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACTCTCCCAGGCCGTCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCcg           <  1:4960152/125‑1 (MQ=255)
                                                                                                          gccAGATCAAGCGGTTCGTCTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGc             <  2:4960151/123‑1 (MQ=255)
                                                                                                            cAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCcgac         <  1:4960155/125‑1 (MQ=255)
                                                                                                             aGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCcgac         >  2:4960288/1‑124 (MQ=255)
                                                                                                              gATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCcgacg        >  1:4960297/1‑124 (MQ=255)
                                                                                                              gATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCCGTCCGGGCTGCcgacg        >  1:4960559/1‑124 (MQ=255)
                                                                                                                  aaGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTATGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCCGACGATGGc   <  2:4960158/125‑1 (MQ=255)
                                                                                                                    gCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGcc            >  2:4960465/1‑114 (MQ=255)
                                                                                                                        |                                                                                                                       
AGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCCGACGATGGCC  >  AE015451/5740435‑5740674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑