Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451 336,125 +T coding (210/237 nt) PP_0278 → conserved protein of unknown function

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451336,1241.T99.2% 426.3 / NA 130D70V (GAT→GTT) PP_0278conserved protein of unknown function
Reads supporting (aligned to +/- strand):  ref base . (0/1);  major base T (31/97);  minor base G (0/1);  total (31/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

GCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GA‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTG  >  AE015451/336001‑336239
                                                                                                                             |                                                                                                                   
gcgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑Ga                                                                                                                      <  2:288949/124‑1 (MQ=255)
  gTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GA‑t                                                                                                                    >  2:289149/1‑123 (MQ=255)
     gagCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GA‑tta                                                                                                                  >  1:289119/1‑120 (MQ=255)
      agCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GA‑ttacct                                                                                                               <  2:288953/124‑6 (MQ=255)
      agCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GA‑tta                                                                                                                  <  1:288954/121‑3 (MQ=255)
       gCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GA‑ttacct                                                                                                               >  2:289138/1‑118 (MQ=255)
        cccGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGCGGACGACCATAAATTCCTGGGGGCAGCC‑GA‑tt                                                                                                                   <  2:288956/118‑2 (MQ=255)
        cccGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCtgt                                                                                                              <  1:288955/124‑1 (MQ=255)
        cccGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCtg                                                                                                               <  1:288957/123‑1 (MQ=255)
            gCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCACGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑Ga                                                                                                                      >  1:289141/1‑112 (MQ=255)
             cGTCCCGGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGa                                                                                                          <  2:288960/123‑1 (MQ=255)
                 cccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACa                                                                                                        <  1:288963/121‑1 (MQ=255)
                  ccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATgtc                                                                                                    <  2:288965/124‑3 (MQ=255)
                  ccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGc                                                                                                    <  1:288964/124‑1 (MQ=255)
                    gTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGcc                                                                                                   >  1:289152/1‑123 (MQ=255)
                    gTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCa                                                                                                  <  2:288966/124‑1 (MQ=255)
                          gcgGCCTACGCGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAgg                                                                                               <  2:288969/121‑1 (MQ=255)
                          gcgGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGgcgc                                                                                            <  2:288970/124‑1 (MQ=255)
                           cgGCCTACGGGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGa                                                                                                          <  1:288973/109‑1 (MQ=255)
                           cgGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCa                                                                                                  <  2:288972/117‑1 (MQ=255)
                           cgGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCt                                                                                           <  1:288971/124‑1 (MQ=255)
                            ggCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCt                                                                                           <  1:288974/123‑1 (MQ=255)
                              ccTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCtgat                                                                                        <  2:288976/124‑1 (MQ=255)
                               cTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCtgatg                                                                                       <  1:288979/124‑1 (MQ=255)
                               cTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATCGATGAAGACCATAAGTTCCTGAGGGCAGCC‑GATTACCTGTGCGATTTGGCCAAGGCGCtgatg                                                                                       <  1:288980/124‑1 (MQ=255)
                                  cGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAAc                                                                                    >  1:289169/1‑124 (MQ=255)
                                   gAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATgg                                                                                                     <  2:288982/106‑1 (MQ=255)
                                   gAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACg                                                                                   >  1:289094/1‑124 (MQ=255)
                                    aGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGa                                                                                  <  1:288983/124‑1 (MQ=255)
                                      cATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACTATAAATTCCTGGGGGCAGCC‑GATTACTTGTGCGACATGGCCAAGGCGCTGATGAACGata                                                                                <  1:288986/124‑1 (MQ=255)
                                      cATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGata                                                                                <  2:288987/124‑1 (MQ=255)
                                      cATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGat                                                                                 <  2:288985/123‑1 (MQ=255)
                                       aTGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGa                                                                                  <  2:288988/121‑1 (MQ=255)
                                         gTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc                                                                              <  1:288989/123‑1 (MQ=255)
                                         gTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc                                                                              <  2:288991/123‑1 (MQ=255)
                                         gTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGAGATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAgg                                                                                               <  2:288992/106‑1 (MQ=255)
                                            tcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGatat                                                                               <  2:288995/119‑1 (MQ=255)
                                              gATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGAt                                                                         <  1:288996/123‑1 (MQ=255)
                                               aTCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGAt                                                                         >  2:289093/1‑122 (MQ=255)
                                                tCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGc                                                                      >  1:289148/1‑124 (MQ=255)
                                                 ccTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCgaga                                                                          <  2:288998/119‑1 (MQ=255)
                                                 ccTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGAt                                                                         <  2:288997/120‑1 (MQ=255)
                                                  cTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCa                                                                    <  1:289000/124‑1 (MQ=255)
                                                   tGATGGTCTACATCAAGCACCTGTTGCGCGAGGGCGAGATGGAGGACGACCATAAATTCCTGGGGGCAGCCGGT‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATc                                                                        <  2:289001/119‑1 (MQ=255)
                                                   tGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATGTGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCaa                                                                   <  2:289002/124‑1 (MQ=255)
                                                      tGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGGTATCGAGATCGcc                                                                     >  2:289117/1‑119 (MQ=255)
                                                       gggCTACATCGAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGt                                                               <  1:289004/124‑1 (MQ=255)
                                                        ggCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGt                                                               >  2:289183/1‑123 (MQ=255)
                                                        ggCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATAAACGATTTCGAGATCGCCAAggat                                                               >  2:289248/1‑121 (MQ=255)
                                                         gCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTaa                                                             <  1:289007/124‑1 (MQ=255)
                                                         gCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTaa                                                             <  1:289006/124‑1 (MQ=255)
                                                           tACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAgg                                                           <  2:289008/124‑1 (MQ=255)
                                                             cATCAAGCACCTGGTGCGCGAGCGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAAATCGCCAAGGGTAAGgcg                                                         <  2:289010/124‑1 (MQ=255)
                                                              aTCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGgcgc                                                        <  2:289012/124‑1 (MQ=255)
                                                              aTCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GAGTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGgcgc                                                        <  1:289011/124‑1 (MQ=255)
                                                                cAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCa                                                       <  1:289013/123‑1 (MQ=255)
                                                                 aaGCACCGGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCAt                                                      <  2:289015/123‑1 (MQ=255)
                                                                  aGCACCTGGTGCGCGAGGGCGATATTGAGGACGACCATAAATTCCTGGGGGCATCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTa                                                    <  1:289017/124‑1 (MQ=255)
                                                                    cACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAg                                                   <  2:289018/123‑1 (MQ=255)
                                                                     aCCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGc                                                 <  1:289019/124‑1 (MQ=255)
                                                                      ccTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGc                                                 <  1:289020/123‑1 (MQ=255)
                                                                      ccTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGc                                                 >  1:289415/1‑123 (MQ=255)
                                                                       cTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCa                                                <  1:289022/123‑1 (MQ=255)
                                                                         ggTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACATGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGCGTAACGCGCATTAGGCa                                                <  1:289023/121‑1 (MQ=255)
                                                                             cgcgAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTa                                                              <  2:289028/103‑1 (MQ=255)
                                                                             cgcgAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGAcgc                                         >  2:289161/1‑124 (MQ=255)
                                                                             cgcgAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGAcgc                                         <  2:289027/124‑1 (MQ=255)
                                                                             cgcgAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGAcgc                                         <  2:289026/124‑1 (MQ=255)
                                                                               cgAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGAcgcg                                        <  1:289030/123‑1 (MQ=255)
                                                                               cgAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGAcgcg                                        <  2:289031/123‑1 (MQ=255)
                                                                               cgAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCgg                                       <  1:289032/124‑1 (MQ=255)
                                                                                 aGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCgg                                       <  1:289033/122‑1 (MQ=255)
                                                                                 aGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGt                                      >  2:289207/1‑123 (MQ=255)
                                                                                 aGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTc                                     >  1:289042/1‑124 (MQ=255)
                                                                                    gCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCa                                    >  1:289165/1‑122 (MQ=255)
                                                                                    gCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCAtt                                  <  1:289035/124‑1 (MQ=255)
                                                                                     cGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCAttt                                 <  2:289036/124‑1 (MQ=255)
                                                                                       atatGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCAttt                                 >  1:289236/1‑122 (MQ=255)
                                                                                            gaggaCGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAg                          <  1:289038/124‑1 (MQ=255)
                                                                                               gacgacCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCgg                       >  2:289189/1‑124 (MQ=255)
                                                                                               gacgacCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCg                        <  1:289040/123‑1 (MQ=255)
                                                                                                acgacCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGt                                      <  1:289041/108‑1 (MQ=255)
                                                                                                      aTAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCg                <  2:289042/124‑1 (MQ=255)
                                                                                                        aaaTTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTc              <  2:289044/124‑1 (MQ=255)
                                                                                                         aaTTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGt               <  1:289045/122‑1 (MQ=255)
                                                                                                         aaTTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTcg             <  2:289046/124‑1 (MQ=255)
                                                                                                          aTTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCTAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTcgc            >  2:289150/1‑124 (MQ=255)
                                                                                                          aTTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTcgc            >  1:289201/1‑124 (MQ=255)
                                                                                                          aTTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTcgc            <  1:289047/124‑1 (MQ=255)
                                                                                                             ccTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGt               <  2:289049/118‑1 (MQ=255)
                                                                                                              cTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGaa         <  1:289050/123‑1 (MQ=255)
                                                                                                              cTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAAt        <  2:289051/124‑1 (MQ=255)
                                                                                                               tGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGaa         <  2:289054/122‑1 (MQ=255)
                                                                                                               tGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATg       <  2:289053/124‑1 (MQ=255)
                                                                                                                 ggggCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGaa         >  2:289166/1‑120 (MQ=255)
                                                                                                                 ggggCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATggg     <  1:289056/124‑1 (MQ=255)
                                                                                                                 ggggCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATgg      <  2:289055/123‑1 (MQ=255)
                                                                                                                 ggggCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATgg      <  1:289057/123‑1 (MQ=255)
                                                                                                                   ggCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATgg      >  1:289184/1‑121 (MQ=255)
                                                                                                                     cAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGTACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTg  >  2:289192/1‑123 (MQ=255)
                                                                                                                             |                                                                                                                   
GCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GA‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTG  >  AE015451/336001‑336239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑