| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 4,808,180 | 0 | A | . | 57.1% | 1.1 / 37.1 | 17 | intergenic (‑289/‑266) | PP_4234/dsbD‑II | Aldehyde oxidase and xanthine dehydrogenase/thiol/disulfide interchange protein 2 |
| Reads supporting (aligned to +/- strand): ref base A (3/0); major base . (2/6); minor base C (0/6); total (5/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCC > AE015451/4808062‑4808303 | ggTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGgcgc < 1:3613018/124‑1 (MQ=255) gTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGgcgct < 2:3613020/124‑2 (MQ=255) gtgtgtGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGgcgcttcg < 2:3613026/123‑5 (MQ=37) gtgtTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGgcgctcga < 1:3613024/123‑5 (MQ=255) tttGGCGGGAGGTTTGCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCgcgg < 2:3613028/123‑1 (MQ=37) ggCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCgcgg < 1:3613030/120‑1 (MQ=37) gtgtTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAacac > 1:3613046/1‑124 (MQ=17) gtgtTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAac < 2:3613036/122‑1 (MQ=17) gggggATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGTGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAaca < 2:3613038/114‑1 (MQ=9) ggggATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTc < 1:3613039/124‑1 (MQ=18) tttCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGc < 1:3613040/124‑1 (MQ=18) cgcCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCa < 1:3613041/114‑1 (MQ=12) gcCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCa > 1:3613118/1‑123 (MQ=17) gcCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCAc < 2:3613042/124‑1 (MQ=17) ggtttacccgcGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTc > 1:3613100/7‑123 (MQ=255) acccgcGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCCAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAAcgc > 2:3613122/2‑124 (MQ=255) acccgcGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAAcgc > 2:3613085/2‑124 (MQ=255) aaGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTcc > 1:3613062/1‑124 (MQ=255) aaGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTcc > 2:3613108/1‑124 (MQ=255) aaGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTcc > 2:3613158/1‑124 (MQ=255) | GGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCC > AE015451/4808062‑4808303 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |