BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	1,499,498	0	T	G	71.5%	74.6 / 145.2	159	intergenic (+75/‑89)	trpS/zapE	Tryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (52/61); minor base C (16/29); total (68/91)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.29e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 159 total aligned reads displayed.

CAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑T‑CGGGCACG‑C‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATC  >  AE015451/1499385‑1499608

 agagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCAcg‑c                                                                                                        <  2:1229822/123‑1 (MQ=25)

 agagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCAc                                                                                                           <  2:1229821/121‑1 (MQ=21)

  gagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAcg‑c‑cc                                                                                                     >  1:1229880/1‑123 (MQ=21)

  gagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCAcg‑c‑c                                                                                                      >  1:1230053/1‑123 (MQ=21)

     ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAcg‑c‑ccgct                                                                                                  >  1:1230116/1‑120 (MQ=21)

     ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAcg‑c‑cc                                                                                                     <  2:1229824/121‑2 (MQ=21)

        gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTcc                                                                                                 >  1:1230191/1‑123 (MQ=18)

         aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTcc                                                                                                 >  2:1229972/1‑122 (MQ=18)

          aCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCca                                                                                               >  1:1230037/1‑123 (MQ=18)

          aCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCca                                                                                               >  1:1230068/1‑123 (MQ=18)

            gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAg                                                                                            >  2:1229984/1‑124 (MQ=18)

             aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCca                                                                                               >  1:1230034/1‑120 (MQ=18)

             aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCca                                                                                               >  2:1229942/1‑120 (MQ=18)

                ccTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCTGGTGTTCG‑CGGGCCCGCC‑CGCTCCCACAGa                                                                                           <  2:1229826/121‑1 (MQ=17)

                ccTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACt                                                                                         >  1:1230059/1‑123 (MQ=18)

                 cTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACt                                                                                         >  1:1230151/1‑122 (MQ=18)

                  ttGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGAt                                                                                      >  1:1230184/1‑124 (MQ=18)

                   tGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACG‑CTCGCTCCCACAGACTGAt                                                                                      <  1:1229827/123‑1 (MQ=18)

                    ggCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGAt                                                                                      <  1:1229828/122‑1 (MQ=18)

                    ggCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGAt                                                                                      <  1:1229830/122‑1 (MQ=18)

                    ggCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTg                                                                                    >  2:1230044/1‑124 (MQ=18)

                     gCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGa                                                                                           <  1:1229836/116‑1 (MQ=17)

                     gCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTg                                                                                    <  2:1229835/123‑1 (MQ=18)

                     gCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTAG‑CGGGCACGCC‑CGCTCCCACAGACTGAt                                                                                      <  1:1229834/121‑1 (MQ=18)

                     gCACCACAGCAGGACTGAGGCTGCCAAACGGTGGCAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAcg‑c‑ccgctc                                                                                                 <  1:1229831/109‑6 (MQ=17)

                        ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCAt                                                                                >  1:1230000/1‑124 (MQ=18)

                            agcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAg                                                                            >  2:1230074/1‑124 (MQ=18)

                             gcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAg                                                                            >  2:1230062/1‑123 (MQ=18)

                             gcagGACTGAGGATGCCAAACGGAGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCGCCGGATTTGCCGATGTTCA‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAg                                                                            <  2:1229838/123‑1 (MQ=18)

                              cagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTaa                                                                             <  2:1229841/121‑1 (MQ=18)

                              cagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTaa                                                                             <  2:1229842/121‑1 (MQ=18)

                              cagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGc                                                                           <  2:1229839/123‑1 (MQ=18)

                                ggACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCCTTAAGCggg                                                                        >  2:1230055/1‑124 (MQ=17)

                                   cTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGc                                                                       <  1:1229845/122‑1 (MQ=18)

                                     gAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCCCGCC‑CGCTcc                                                                                                 >  2:1230113/1‑94 (MQ=11)

                                     gAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTa                                                                    <  2:1229846/123‑1 (MQ=18)

                                      aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTa                                                                    <  1:1229849/122‑1 (MQ=18)

                                      aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTAt                                                                   <  2:1229850/123‑1 (MQ=18)

                                        gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGc                                                                <  2:1229852/124‑1 (MQ=18)

                                        gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTa                                                                              <  1:1229851/110‑1 (MQ=14)

                                         cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCt                                                               <  1:1229854/124‑1 (MQ=18)

                                          tGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa                                                              <  1:1229856/124‑1 (MQ=18)

                                            ccAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTAc                                                             <  2:1229858/123‑1 (MQ=18)

                                            ccAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa                                                            <  1:1229859/124‑1 (MQ=18)

                                                 cGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGGT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGa                                                        <  1:1229862/123‑1 (MQ=18)

                                                  ggTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGc                                                                <  2:1229864/114‑1 (MQ=17)

                                                  ggTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGa                                                        >  1:1230078/1‑122 (MQ=18)

                                                   gTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTa                                                                    <  2:1229866/109‑1 (MQ=14)

                                                    tGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACg                                                      <  1:1229867/122‑1 (MQ=18)

                                                      ggAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGcc                                                   <  2:1229869/123‑1 (MQ=18)

                                                      ggAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGcc                                                   <  2:1229870/123‑1 (MQ=18)

                                                       gAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACCGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc                                                  >  1:1229977/1‑123 (MQ=18)

                                                          cgggcgggCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtt                                               <  1:1229874/123‑1 (MQ=18)

                                                          cGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtt                                               <  2:1229875/123‑1 (MQ=18)

                                                          cGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCAGGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTa                                                                    <  1:1229873/102‑1 (MQ=14)

                                                              cGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc                                                  >  1:1230054/1‑116 (MQ=18)

                                                              cGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCGACAGTGACGGCCCGTTTTttg                                          >  2:1230065/1‑124 (MQ=21)

                                                                tGCCCGCGATACAGGCGCCGCGGTGCATGGCACCGGCTTTGCAGGTGTTCG‑CGGGCGCGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGttttt                                            <  2:1229877/120‑1 (MQ=21)

                                                                tGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt                                         >  1:1230260/1‑123 (MQ=21)

                                                                tGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttg                                          >  2:1230156/1‑122 (MQ=21)

                                                                  cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGc                                      >  1:1229987/1‑124 (MQ=21)

                                                                  cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGAGTGATTGCCATTAAGCGGGCCTGTCGCAACAGTGGCGGCCCGTTTTTTGTTGc                                      >  2:1230118/1‑124 (MQ=18)

                                                                   ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGcc                                     <  2:1229880/124‑1 (MQ=21)

                                                                    cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc                                                  >  1:1230091/1‑110 (MQ=21)

                                                                    cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCt                                    <  2:1229883/124‑1 (MQ=25)

                                                                    cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCt                                    >  1:1230121/1‑124 (MQ=25)

                                                                      cgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCg                                  <  2:1229884/124‑1 (MQ=25)

                                                                       gAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCGCCCACCGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGGTTTTTGTTGCCTCg                                  >  2:1230047/1‑123 (MQ=21)

                                                                         aaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCt                                    <  1:1229887/119‑1 (MQ=25)

                                                                         aaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTa                               <  1:1229886/124‑1 (MQ=25)

                                                                          aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa                              <  2:1229888/124‑1 (MQ=25)

                                                                           cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGCTTTTTGTTGCCTCGCTa                               >  1:1230106/1‑122 (MQ=25)

                                                                            aGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTa                               <  2:1229889/121‑1 (MQ=25)

                                                                              gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGcc                                                                      >  1:1230309/1‑80 (MQ=14)

                                                                              gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGaa                          <  1:1229891/124‑1 (MQ=25)

                                                                              gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGGTTTTTGTTGCCTCg                                  >  2:1230153/1‑116 (MQ=21)

                                                                                gccgcGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt                         <  1:1229893/123‑1 (MQ=25)

                                                                                  cgcgGTGCATGGCACCGGCTTTGCCGGTGTTCG‑GGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc                        <  2:1229895/122‑1 (MQ=25)

                                                                                  cgcgGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  1:1229898/123‑1 (MQ=25)

                                                                                  cgcgGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  2:1229899/123‑1 (MQ=25)

                                                                                  cgcgGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  2:1229897/123‑1 (MQ=25)

                                                                                    cgGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc                                                  >  2:1230215/1‑94 (MQ=18)

                                                                                    cgGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa                    >  1:1230125/1‑124 (MQ=25)

                                                                                     ggTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa                    >  1:1230107/1‑123 (MQ=25)

                                                                                       tGCATGGCACCGGCTTTGCCGGAGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt                         >  2:1230081/1‑116 (MQ=25)

                                                                                        gCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc                 <  1:1229901/123‑1 (MQ=25)

                                                                                            ggcaccGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc                 <  2:1229903/114‑1 (MQ=37)

                                                                                            ggcaccGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa            <  1:1229904/119‑1 (MQ=37)

                                                                                             gcaccGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa            >  2:1230095/5‑123 (MQ=37)

                                                                                                ccGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc                                                  >  1:1230354/2‑82 (MQ=21)

                                                                                                ccGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa         <  2:1229905/122‑1 (MQ=38)

                                                                                                ccGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa         >  1:1230093/2‑123 (MQ=38)

                                                                                                 cGGCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg       >  1:1230155/1‑124 (MQ=38)

                                                                                                   gCTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGc      >  1:1230043/1‑123 (MQ=38)

                                                                                                    cTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa    <  1:1229909/124‑1 (MQ=38)

                                                                                                    cTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa    <  2:1229910/124‑1 (MQ=38)

                                                                                                    cTTTGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa    <  2:1229908/124‑1 (MQ=38)

                                                                                                      ttGCCGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt     <  1:1229911/121‑1 (MQ=38)

                                                                                                       tGCCGGTGTTCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc  <  1:1229913/123‑1 (MQ=38)

                                                                                                         ccGGTGTT‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa         <  1:1229914/114‑1 (MQ=38)

CAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑T‑CGGGCACG‑C‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATC  >  AE015451/1499385‑1499608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`