Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451 5,740,555 C→T S175N (AGC→AAC)  PP_5037 ← lipocalin family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154515,740,5550CT100.0% 540.3 / NA 175S175N (AGC→AAC) PP_5037lipocalin family lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (92/83);  total (92/83)

Only 100 of 175 total aligned reads displayed.

AGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCCGACGAT  >  AE015451/5740435‑5740670
                                                                                                                        |                                                                                                                   
  cGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGgtg                                                                                                                <  1:4385712/124‑1 (MQ=255)
   gTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGgt                                                                                                                 <  1:4385713/122‑1 (MQ=255)
   ctACCGGGGACGCGTCGCAGCCCTTCGCGTGCTAGCCCGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCCGATCGGACGCCCGCCAGATCAAGCGGTTGgtgt                                                                                                               >  2:4385817/2‑124 (MQ=37)
     aCCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTc                                                                                                              >  2:4385756/1‑123 (MQ=255)
        ggggTTGCGTTGCAGCCCTTCGCGTGCAAGACCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCAt                                                                                                            >  1:4385945/1‑122 (MQ=255)
         gggTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATAcc                                                                                                         <  1:4385716/124‑1 (MQ=255)
           gttgcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCAt                                                                                                            >  1:4385903/1‑119 (MQ=255)
           gttgcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATAcccc                                                                                                       <  2:4385718/124‑1 (MQ=255)
           gttgcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATAcccc                                                                                                       <  1:4385719/124‑1 (MQ=255)
            ttgcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCct                                                                                                      <  1:4385721/124‑1 (MQ=255)
               cgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCctgc                                                                                                    <  1:4385723/123‑1 (MQ=255)
                  tgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAGCCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCctgc                                                                                                    <  1:4385727/120‑1 (MQ=255)
                   gcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCtgctgc                                                                                                 <  2:4385728/122‑1 (MQ=255)
                    cAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAACAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCcg                                                                                               <  2:4385729/123‑1 (MQ=255)
                    cAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCcg                                                                                               >  2:4385957/1‑123 (MQ=255)
                         cTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCt                                                                                         >  2:4385790/1‑124 (MQ=255)
                         cTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCt                                                                                         <  1:4385732/124‑1 (MQ=255)
                             gcgTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCt                                                                                         <  2:4385735/120‑1 (MQ=255)
                              cgTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGcc                                                                                           >  1:4385861/1‑117 (MQ=255)
                              cgTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGAc                                                                                     >  2:4385863/1‑123 (MQ=255)
                                tGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcag                                                                                   >  1:4385901/1‑123 (MQ=255)
                                  cAAGCCCGCGAAGGGTTGCAACGCACCCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGcc                                                                                                <  2:4385737/108‑1 (MQ=255)
                                        cgcgATGGGTTGCAACGCACCCCCAAAAAACCCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtct                                                                           <  2:4385739/123‑1 (MQ=255)
                                         gcgAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa                                                                         >  1:4385989/1‑124 (MQ=255)
                                         gcgAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa                                                                         <  2:4385740/124‑1 (MQ=255)
                                          cgAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGAca                                                                                    <  2:4385743/112‑1 (MQ=255)
                                          cgAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtctc                                                                          <  1:4385741/122‑1 (MQ=255)
                                           gAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACg                                                                       >  1:4385820/1‑124 (MQ=255)
                                           gAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACg                                                                       >  2:4385866/1‑124 (MQ=255)
                                           gAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTCATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcag                                                                                   >  2:4385956/1‑112 (MQ=255)
                                            aaGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACg                                                                       >  1:4386064/1‑123 (MQ=255)
                                            aaGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGc                                                                      <  1:4385744/124‑1 (MQ=255)
                                              gggTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTc                                                                    >  2:4385914/1‑124 (MQ=255)
                                               ggTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc                                                                   >  1:4385893/1‑124 (MQ=255)
                                                gTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc                                                                   >  1:4385992/1‑123 (MQ=255)
                                                 ttGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCa                                                                 >  1:4386036/1‑124 (MQ=255)
                                                 ttGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCa                                                                 <  1:4385746/124‑1 (MQ=255)
                                                 ttGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCa                                                                 >  1:4385888/1‑124 (MQ=255)
                                                     aacgcaacCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATa                                                                                                           <  2:4385750/78‑1 (MQ=255)
                                                     aacgcaacCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGcc                                                             <  1:4385749/124‑1 (MQ=255)
                                                      acgcaacCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCg                                                            >  2:4385876/1‑124 (MQ=255)
                                                       cgcaacCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCgg                                                           >  1:4386019/1‑124 (MQ=255)
                                                       cgcaacCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCCCACGCTccc                                                                  >  2:4386157/1‑117 (MQ=255)
                                                           acCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCa                                                         >  1:4386119/1‑122 (MQ=255)
                                                             cccAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGAAAGCAGGTTCTCACGCTCCCAGGCCGGCAgtgg                                                     <  1:4385753/124‑2 (MQ=255)
                                                              ccAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCtgctgc                                                                                                 <  1:4385754/79‑1 (MQ=255)
                                                               cAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCg                                                   >  2:4385853/1‑124 (MQ=255)
                                                                aaaaaaCTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCgg                                                  <  1:4385756/124‑1 (MQ=255)
                                                                 aaaaaCTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGgt                                                 >  1:4385849/1‑124 (MQ=255)
                                                                  aaaaCTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGgtg                                                <  1:4385758/124‑1 (MQ=255)
                                                                   aaaCTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGgtgt                                               >  1:4385993/1‑124 (MQ=255)
                                                                   aaaCTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGgtgt                                               <  2:4385759/124‑1 (MQ=255)
                                                                    aaCTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGcc                                                                                           >  2:4385881/1‑79 (MQ=255)
                                                                      cTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTgcg                                            <  1:4385764/124‑1 (MQ=255)
                                                                      cTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTgcg                                            <  2:4385763/124‑1 (MQ=255)
                                                                      cTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTgc                                             >  2:4385952/1‑123 (MQ=255)
                                                                       tCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGgtg                                                <  1:4385765/119‑1 (MQ=255)
                                                                         aGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCctgc                                                                                                    <  1:4385768/65‑1 (MQ=255)
                                                                         aGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGa                                         >  1:4385787/1‑124 (MQ=255)
                                                                         aGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGa                                         >  1:4385996/1‑124 (MQ=255)
                                                                           tGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACa                                       <  1:4385770/124‑1 (MQ=255)
                                                                            gCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCGAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAg                                      >  1:4385933/1‑124 (MQ=255)
                                                                            gCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCg                                                            <  2:4385771/102‑1 (MQ=255)
                                                                              aTTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTgcgc                                           >  1:4386114/1‑117 (MQ=255)
                                                                                ttttACTATTTGCTGTTCTGACGCCAGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATc                                  <  2:4385773/124‑1 (MQ=255)
                                                                                ttttACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGGGTGCGCGACAGAATc                                  >  1:4386015/1‑124 (MQ=255)
                                                                                 tttACTATTCGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc                                                              >  1:4386018/1‑95 (MQ=255)
                                                                                  ttACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCa                                <  2:4385774/124‑1 (MQ=255)
                                                                                     cTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAAt                                   >  2:4385937/1‑118 (MQ=255)
                                                                                     cTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAg                             >  2:4385965/1‑124 (MQ=255)
                                                                                      tATTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCctgct                                                                                                   <  1:4385777/53‑1 (MQ=255)
                                                                                       aTTTGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGa                                     >  2:4386028/1‑114 (MQ=255)
                                                                                         ttGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAgg                            <  1:4385778/121‑1 (MQ=255)
                                                                                         ttGCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGAGCGCGACAGAATCCAAAGGTAt                         >  1:4385923/1‑124 (MQ=255)
                                                                                           gCTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAgg                                                                               >  2:4386020/1‑68 (MQ=255)
                                                                                            cTGATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTAt                         <  2:4385779/121‑1 (MQ=255)
                                                                                              gATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCg                    >  2:4385987/1‑124 (MQ=255)
                                                                                              gATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCg                    >  2:4385897/1‑124 (MQ=255)
                                                                                              gATCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCAGTGTGCGCGACAGAATCCAAAGGTATTTGCg                    >  2:4385877/1‑124 (MQ=255)
                                                                                               aTCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTg                      <  1:4385781/121‑1 (MQ=255)
                                                                                               aTCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCgg                   <  1:4385783/124‑1 (MQ=255)
                                                                                                tCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGc                     >  2:4385846/1‑121 (MQ=255)
                                                                                                tCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGt                  >  1:4385974/1‑124 (MQ=255)
                                                                                                tCGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGt                  >  1:4385949/1‑124 (MQ=255)
                                                                                                 cGGACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTc                 >  1:4385938/1‑124 (MQ=255)
                                                                                                  ggACGCCCGCCAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCa                                <  2:4385785/108‑1 (MQ=255)
                                                                                                     cgcccgccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCg                    >  2:4386043/1‑117 (MQ=255)
                                                                                                      gcccgccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGc            <  2:4385788/124‑1 (MQ=255)
                                                                                                      gcccgccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGc            <  1:4385789/124‑1 (MQ=255)
                                                                                                        ccgccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTg          <  1:4385792/124‑1 (MQ=255)
                                                                                                          gccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCggg             <  2:4385793/119‑1 (MQ=255)
                                                                                                           ccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGa                                                                                      >  2:4386091/1‑45 (MQ=255)
                                                                                                           ccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGcc        >  2:4386076/1‑123 (MQ=255)
                                                                                                           ccAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCAGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGcc        >  2:4385916/1‑123 (MQ=255)
                                                                                                            cAGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCACCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCcga      <  1:4385796/124‑1 (MQ=255)
                                                                                                             aGATCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAAACCAAAGGTATTTGCGGTCCGGGCTGCcgac     >  2:4385999/1‑124 (MQ=255)
                                                                                                               aTCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCcg       >  2:4385959/1‑120 (MQ=255)
                                                                                                                tCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCCGACGAt  >  1:4385961/1‑124 (MQ=255)
                                                                                                                tCAAGCGGTTGGTGTCATACCCCTGCTGCCGCGACCTGTACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCCGACGAt  >  1:4386350/1‑124 (MQ=255)
                                                                                                                     cGGTTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCcga      <  1:4385798/115‑1 (MQ=255)
                                                                                                                        |                                                                                                                   
AGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGCGACAGAATCCAAAGGTATTTGCGGTCCGGGCTGCCGACGAT  >  AE015451/5740435‑5740670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑