| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 4,808,171 | 0 | C | . | 63.6% | ‑5.6 / 16.1 | 11 | intergenic (‑280/‑275) | PP_4234/dsbD‑II | Aldehyde oxidase and xanthine dehydrogenase/thiol/disulfide interchange protein 2 |
| Reads supporting (aligned to +/- strand): ref base C (0/4); new base . (2/5); total (2/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTGTGTATAGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCC > AE015451/4808023‑4808279 | ggTGTGTATAGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGcgccg < 1:4092511/151‑1 (MQ=255) gtgtATAGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGccgccc < 1:4092512/151‑1 (MQ=255) gtgtATAGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGccgccc < 1:4092513/151‑1 (MQ=255) tgtATAGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGccgcccg < 2:4092514/151‑1 (MQ=255) gtATAGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGccgcgcgg > 1:4092551/1‑150 (MQ=255) gtATAGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGccgcgcgg < 2:4092515/151‑2 (MQ=255) tataGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGcgccgcgcggg < 1:4092516/151‑6 (MQ=255) tataGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGccgccccc > 2:4092593/1‑148 (MQ=255) ataGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCcgcg < 2:4092517/150‑1 (MQ=255) taGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGcgccgcgcgggcg < 2:4092518/151‑8 (MQ=255) gggCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCcgcgcggc < 1:4092519/151‑5 (MQ=255) gCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCt < 2:4092531/151‑1 (MQ=25) cAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAAc < 1:4092532/151‑1 (MQ=25) cgcCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCaaaa < 1:4092533/141‑1 (MQ=21) aGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTc > 1:4092572/1‑151 (MQ=21) gtgtTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCaa > 2:4092569/1‑151 (MQ=21) gCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCa < 2:4092534/140‑1 (MQ=21) gggggATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAAc < 1:4092535/151‑1 (MQ=25) gggggATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCGGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAAc < 2:4092536/151‑1 (MQ=21) tttCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGccc < 2:4092537/151‑1 (MQ=21) | GGTGTGTATAGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCC > AE015451/4808023‑4808279 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 44 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |