Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451 2,445,964 G→T E80* (GAG→TAG)  PP_2144 → Transcriptional regulator, TetR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154512,445,9640GT100.0% 1145.7 / NA 272E80* (GAG→TAG) PP_2144Transcriptional regulator, TetR family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (158/114);  total (158/114)

Only 100 of 272 total aligned reads displayed.

GATTACCAGCAAGGCCGGGGTCAACCTGGCGGCAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGGAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCAGCCAGAGCCAGGGTCACCTGCGGCGCTATC  >  AE015451/2445816‑2446111
                                                                                                                                                    |                                                                                                                                                   
gATTACCAGCAAGGCCGGGGTCAACCTGGCGGCAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAg                                                                                                                                                   <  1:2092701/151‑1 (MQ=255)
          aaGGCCGGGGTCAACCTGGCGGCAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAg                                                                                                                                                   >  2:2092993/1‑141 (MQ=255)
          aaGGCCGGGGTCAACCTGGCGGCAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGccc                                                                                                                                          >  2:2092958/1‑150 (MQ=255)
          aaGGCCGGGGTCAACCTGGCGGCAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCa                                                                                                                                         <  2:2092705/151‑1 (MQ=255)
           aGGCCGGGGTCAACCTGGCGGCAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCTAGGCCAAGCCGTAGCACAAGCCCAg                                                                                                                                        <  1:2092706/151‑1 (MQ=255)
            ggCCGGGGTCAACCTGGCGGCAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGc                                                                                                                                       >  2:2093019/1‑151 (MQ=255)
             gCCGGGGTCAACCTGGCGGCAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGcc                                                                                                                                      <  2:2092709/151‑1 (MQ=255)
                      aaCCTGGCGGCAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAAc                                                                                                                             >  1:2093002/1‑151 (MQ=255)
                      aaCCTGGCGGCAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAAc                                                                                                                             <  2:2092713/151‑1 (MQ=255)
                       aCCTGGCGGCAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAAct                                                                                                                            >  1:2092921/1‑151 (MQ=255)
                       aCCTGGCGGCAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAAct                                                                                                                            <  1:2092714/151‑1 (MQ=255)
                        ccTGGCGGCAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAActg                                                                                                                           >  2:2093213/1‑151 (MQ=255)
                           ggcggcAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAActgctg                                                                                                                        >  1:2092842/1‑151 (MQ=255)
                              gtcAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGaa                                                                                                                     <  2:2092719/149‑1 (MQ=255)
                              ggcAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGaa                                                                                                                     >  1:2093132/1‑151 (MQ=255)
                               gcAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGaaa                                                                                                                    <  1:2092720/151‑1 (MQ=255)
                               gcAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGaaa                                                                                                                    >  2:2092922/1‑151 (MQ=255)
                                cAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACCGCTGGaaa                                                                                                                    >  1:2093049/1‑150 (MQ=255)
                                    cAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTc                                                                                                               <  2:2092723/151‑1 (MQ=255)
                                      aCTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGt                                                                                                             >  1:2092901/1‑151 (MQ=255)
                                       cTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTa                                                                                                            >  2:2093062/1‑151 (MQ=255)
                                        tATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTag                                                                                                           <  1:2092726/151‑1 (MQ=255)
                                        tATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTag                                                                                                           >  1:2093087/1‑151 (MQ=255)
                                         aTCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTaga                                                                                                          <  2:2092728/151‑1 (MQ=255)
                                         aTCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTaga                                                                                                          >  2:2093130/1‑151 (MQ=255)
                                           cttttCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGc                                                                                                        <  1:2092730/149‑1 (MQ=255)
                                           cATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGc                                                                                                        >  2:2092944/1‑151 (MQ=255)
                                           cATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTAGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGc                                                                                                        >  2:2093399/1‑151 (MQ=255)
                                              ttCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAgg                                                                                                     >  1:2092969/1‑151 (MQ=255)
                                                cGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGgcg                                                                                                   >  1:2093060/1‑151 (MQ=255)
                                                   cTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTg                                                                                                <  2:2092735/151‑1 (MQ=255)
                                                   cTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTg                                                                                                >  1:2092861/1‑151 (MQ=255)
                                                    tCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTg                                                                                                <  1:2092738/150‑1 (MQ=255)
                                                     ccAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGc                                                                                              >  1:2092929/1‑151 (MQ=255)
                                                       aagaagGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGcc                                                                                             <  2:2092741/150‑1 (MQ=255)
                                                       aagaagGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCg                                                                                            <  1:2092742/151‑1 (MQ=255)
                                                        agaagGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGt                                                                                           >  1:2093222/1‑151 (MQ=255)
                                                           agGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCa                                                                                        <  1:2092746/151‑1 (MQ=255)
                                                             gcgcTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGc                                                                                      >  2:2092868/1‑151 (MQ=255)
                                                                cTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCcgc                                                                                   <  2:2092749/151‑1 (MQ=255)
                                                                 tGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCcgcg                                                                                  >  1:2093042/1‑151 (MQ=255)
                                                                 tGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCcgc                                                                                   >  2:2093030/1‑150 (MQ=255)
                                                                 tGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCcgc                                                                                   >  2:2093010/1‑150 (MQ=255)
                                                                  gATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCgcgc                                                                                 >  2:2092820/1‑151 (MQ=255)
                                                                   aTCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCgca                                                                                <  2:2092751/151‑1 (MQ=255)
                                                                     ccAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCgcagc                                                                              <  1:2092754/151‑1 (MQ=255)
                                                                      cAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCgcagca                                                                             >  2:2093050/1‑151 (MQ=255)
                                                                          ccGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGcaacaa                                                                         <  2:2092756/151‑1 (MQ=255)
                                                                            gTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACg                                                                       <  1:2092759/151‑1 (MQ=255)
                                                                             ttttCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGa                                                                      >  1:2092933/1‑151 (MQ=255)
                                                                                 ctcGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTg                                                                  >  1:2093053/1‑151 (MQ=255)
                                                                                   cgcgTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGt                                                                 <  1:2092764/150‑1 (MQ=255)
                                                                                   cgcgTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTc                                                                <  2:2092762/151‑1 (MQ=255)
                                                                                    gcgTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCg                                                               <  2:2092766/151‑1 (MQ=255)
                                                                                    gcgTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCg                                                               >  1:2092987/1‑151 (MQ=255)
                                                                                      gTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGAt                                                             >  2:2093155/1‑151 (MQ=255)
                                                                                       tttCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATc                                                            >  1:2093096/1‑151 (MQ=255)
                                                                                        ttCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCt                                                           <  2:2092770/151‑1 (MQ=255)
                                                                                         tCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCtt                                                          >  1:2093144/1‑151 (MQ=255)
                                                                                          ccTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTc                                                         <  1:2092772/151‑1 (MQ=255)
                                                                                          ccTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTc                                                         >  1:2093108/1‑151 (MQ=255)
                                                                                            ttGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCAt                                                       >  2:2093097/1‑151 (MQ=255)
                                                                                              ggCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATgc                                                     <  2:2092775/151‑1 (MQ=255)
                                                                                                cccATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATgcgc                                                   <  1:2092779/151‑1 (MQ=255)
                                                                                                cccATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATgcg                                                    <  2:2092778/150‑1 (MQ=255)
                                                                                                 ccATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGcc                                                  >  2:2093169/1‑151 (MQ=255)
                                                                                                  cATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGcc                                                  >  2:2093199/1‑150 (MQ=255)
                                                                                                      cTGCGCCAGTCTGGGGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGCAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAATAACGACCTGTCGATCTTCATGCGCctgctg                                             >  2:2093306/1‑151 (MQ=255)
                                                                                                      cTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCctgctg                                             >  2:2092953/1‑151 (MQ=255)
                                                                                                      cTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCctgctg                                             >  2:2092893/1‑151 (MQ=255)
                                                                                                        gcgcCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTggg                                           <  1:2092784/151‑1 (MQ=255)
                                                                                                        gcgcCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTgg                                            <  2:2092781/150‑1 (MQ=255)
                                                                                                         cgcCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGc                                          >  1:2093111/1‑151 (MQ=255)
                                                                                                            cAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTg                                       <  1:2092786/151‑1 (MQ=255)
                                                                                                             aGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTgg                                      >  1:2093323/1‑151 (MQ=255)
                                                                                                             aGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTgg                                      >  2:2093089/1‑151 (MQ=255)
                                                                                                              gTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTgg                                      >  2:2093141/1‑150 (MQ=255)
                                                                                                                cTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCTTGGCGt                                   >  1:2093154/1‑151 (MQ=255)
                                                                                                                  ggAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTc                                 >  1:2093274/1‑151 (MQ=255)
                                                                                                                    aGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTc                                                                <  1:2092791/118‑1 (MQ=255)
                                                                                                                    aGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCag                               >  2:2092981/1‑151 (MQ=255)
                                                                                                                    aGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCag                               >  2:2093242/1‑151 (MQ=255)
                                                                                                                       gTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCagcc                             <  2:2092793/150‑1 (MQ=255)
                                                                                                                       gTGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCagcc                             >  2:2093105/1‑150 (MQ=255)
                                                                                                                        tGAGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCagccag                           <  1:2092796/151‑1 (MQ=255)
                                                                                                                          aGCTGGAGCGCCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCagccagag                         >  1:2093406/1‑151 (MQ=255)
                                                                                                                                 gcgcCGCCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCAGCCAGAGCCAggg                   <  2:2092799/150‑1 (MQ=255)
                                                                                                                                  cgccgcCAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCg                                    <  2:2092801/132‑1 (MQ=255)
                                                                                                                                    ccgccAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCAGCCAGAGCCAGGGTCAc               >  1:2093164/1‑151 (MQ=255)
                                                                                                                                    ccgccAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCAGCCAGAGCCAGGGTCAc               >  1:2093126/1‑151 (MQ=255)
                                                                                                                                     cgccAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCAGCCAGAGCCAGGGTCAcc              >  1:2093260/1‑151 (MQ=255)
                                                                                                                                     cgccAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCAGCCAGAGCCAGGGTCAcc              <  1:2092803/151‑1 (MQ=255)
                                                                                                                                     cgccAGGCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCAGCCAGAGCCAGGGTCAcc              >  2:2093190/1‑151 (MQ=255)
                                                                                                                                           gCCAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCAGCCAGAGCCAGGGTCACCTgcggc        <  1:2092807/151‑1 (MQ=255)
                                                                                                                                            ccAAGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCAGCCAGAGCCAGGGTCACCTgcggcg       <  2:2092811/151‑1 (MQ=255)
                                                                                                                                            caaaGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCAGCCAGAGCCAGGGTCACCTgcggc        <  1:2092810/148‑1 (MQ=255)
                                                                                                                                              aaGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCAGCCAGAGCCAGGGTCACCTGCGgcgc      >  2:2093276/1‑150 (MQ=255)
                                                                                                                                               aGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCAGCCAGAGCCAGGGTCACCTGCGGCGCt     >  1:2093031/1‑150 (MQ=255)
                                                                                                                                               aGCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCAGCCAGAGCCAGGGTCACCTGCGGCGCTa    >  2:2092937/1‑151 (MQ=255)
                                                                                                                                                gCCGTAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCAGCCAGAGCCAGGGTCACCTGCGGCGCTa    >  2:2093184/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GATTACCAGCAAGGCCGGGGTCAACCTGGCGGCAGTCAACTATCATTTCGGCTCCAAGAAGGCGCTGATCCAGGCCGTTTTCTCGCGTTTCCTTGGCCCATTCTGCGCCAGTCTGGAGCGTGAGCTGGAGCGCCGCCAGGCCAAGCCGGAGCACAAGCCCAGCCTCGAAGAACTGCTGGAAATGCTCGTAGAGCAGGCGCTGGCCGTGCAGCCGCGCAGCAACAACGACCTGTCGATCTTCATGCGCCTGCTGGGCCTGGCGTTCAGCCAGAGCCAGGGTCACCTGCGGCGCTATC  >  AE015451/2445816‑2446111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 43 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑