Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,604,819 A→T 27.9% N258I (AAT→ATT)  lsrD → Autoinducer‑2 ABC transporter membrane subunit LsrD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,604,8190AT27.9% 10.8 / 3.5 14N258I (AAT→ATT) lsrDAutoinducer‑2 ABC transporter membrane subunit LsrD
Reads supporting (aligned to +/- strand):  ref base A (5/5);  new base T (2/2);  total (7/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01

TTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGG  >  NC_000913/1604549‑1605010
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                               
ttATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCGGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGTGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATAtt                                                                                                                                                                                             <  1:196019/275‑1 (MQ=255)
                                               aCCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTACGATCTCGGTGCGTCGTTTCTGATGCCAGCCATCACCGCCGTGGTGCTTGGCGGGGCAAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTttatt                                                                                                                                              >  1:8917/1‑275 (MQ=255)
                                                                 gtataagcgccagggCAAAGCCCGCGCGTGGCCGTTTATAGCGCGATTGCAGTGAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTTGCGTCTGCGGTCGCCGCTGTGCTGCTGGTGTCGTATTTTGGTTCAGCACGTGCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCGCCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGcaac                                                                                                                             <  1:29225/262‑1 (MQ=255)
                                                                                         cgcgTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGGGCTCGGTGCGTCGCTCCTGATGCCCGCCATCACCGCCGTGGTCCTTGGCGGGGCCGATTTTTCTGGTGGTTCCGGTTCCATTATCGGGCCCGCCCATTAGGTTTTTTTCATGGGATATTTGCAAAGAGGTTTGTATATGGCCgggtgtgc                                                                                                    >  2:51558/1‑269 (MQ=255)
                                                                                         cgcgTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGATATATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTTGTTCAGCAGGTTCCGATCTCGGTGCGTCGTCTCTGATGCCCGTCTTCACCGCCGGTGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCCGTTCCAGTATCGGCCCCGCCATGGCGGTTTGTTGAGGGGGGTATTTGGCACGAGGTTTTCAAATgctttttttatc                                                                                                    >  2:29225/1‑264 (MQ=255)
                                                                                                          cgcgATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGCTGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCATTATTTATGGTGGTTCCGGTTCCATTATCGGCACGGCCATTGCGGTTTTATTAGTGGGAGATTTGCAACCAGGTTTCCAAAGGGCCGGGTTGCCCAATTAGgtgttcgtcg                                                                                   >  2:16761/1‑269 (MQ=255)
                                                                                                          cgcgATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCCTTTTTTATGGTGGTTCCGGTTCCATTATCGGCCCCGCCATTGAGGTTTTATTGGTGGGGTTTTTGCCACCGTGTTTGCAAATGGTCGGGTTGCCCAATcccggggtgcggt                                                                                   >  2:16766/1‑263 (MQ=255)
                                                                                                                                     tgCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCATTATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTAtcgtcg                                                        <  1:16761/275‑1 (MQ=255)
                                                                                                                                     tgCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCATTATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTAtcgtcg                                                        <  1:16766/275‑1 (MQ=255)
                                                                                                                                     tgCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGGCGCCGCTGTGCTGCTGGTATCGTATTTGGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTAtcgtcg                                                        <  1:51558/275‑1 (MQ=255)
                                                                                                                                                              gCGGCTGCGGTCGCCGCGGTGCTGCTTGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGGGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCTGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCAAAATCAGGTGTCCAGCGCCCTTTCCGGTgcg                                                                     <  1:51542/237‑1 (MQ=255)
                                                                                                                                                                                           aTCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCCTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTTCGGTTCCATTATCGGCACCGCCCTTGCGGGTTTATTAGTGGGATATTTGCTACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTCCTTATAGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGtggctgg  >  1:60578/1‑275 (MQ=255)
                                                                                                                                                                                           aTCGTATTTTGGTTCAGCACGTTCCGATATCGGTGCGGCGTTTCTGATGCCCGCCATCACCGCCGGGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGGCAGTGCGGTTGTATTAGTGGGATATTTGCAACAAGGGTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTCCTTAtc                                                            >  1:52173/1‑217 (MQ=255)
                                                                                                                                                                                                                     aTCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCg                                           <  1:195647/208‑1 (MQ=255)
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                               
TTATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGG  >  NC_000913/1604549‑1605010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑