| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,735,574 | C→T | 22.8% | M146I (ATG→ATA) | rluD ← | 23S rRNA pseudouridine(1911/1915/1917) synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,735,574 | 0 | C | T | 22.8% | 27.9 / 2.8 | 17 | M146I (ATG→ATA) | rluD | 23S rRNA pseudouridine(1911/1915/1917) synthase |
| Reads supporting (aligned to +/- strand): ref base C (5/8); new base T (2/2); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
CTGGTGCGTACGTCCAGTTTCCAGACGCAACCGCAGACGCGTGTGCACACGGAAGTGTTCCATGATGCGATAGTGAGTCACCGCTGGTTTGCCCATCGGATGCACCGCCATATGGGTACGTTTGGTCGGGTGGCGACTGATTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAAGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATG > NC_000913/2735307‑2735699 | cTGGTGCGTACGTCCAATTTCCAGACCCAAACGCAGACGCGTGTGCACACGGAAGTTTTCCATGGTGCGATAGTGAGTCACCGCTGGTTTGCCCATCGGAGGCACCGCCATATGGGTACGTTTGGTCGGGTGGCGACTGATTGGCTCGGCCACCGTGCCACCTGCGGTCATATGACCAATCGCCACCGCTTCTTACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGc < 1:24018/275‑1 (MQ=255) gCGATAGTGAGTCACCGCTGGTTTGCCCATCGGATGCACCGCCATATGGGTACGTTTGGTCGGGTGGCGACTGATTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTACACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCa < 1:200759/247‑1 (MQ=255) aCCGCTGGTTTGCCCATCGGATGCACCGCCATATGGGTACGTTTGGTCGGGTGGCGACTGATTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAAGCAAc < 1:169420/273‑1 (MQ=255) gCTGGTTTGCCCATCGGATGCACCGCCATATGGGTANGTTTGGTCGGGTGGCGACTGATTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGATTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCCTCTGAGCCGGAACGGGTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGcc > 1:106220/1‑227 (MQ=255) gTCGGGTGGCGACTGATTGGCTCGTCCACCGTGCCACCTGCTGTCATATGACCAATCTCCACCGCTTCATACTCATGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCa < 1:211068/189‑1 (MQ=255) aCTGATTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACTATCAGGCCa > 2:26141/1‑142 (MQ=255) aCTGATTGGCACGTCCACCGTGCCACCTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACTATCAGGCCa > 2:26143/1‑142 (MQ=255) aTTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACTATCAGGCCAgtgg < 1:26141/142‑1 (MQ=255) aTTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACTATCAGGCCAgtgg < 1:26143/142‑1 (MQ=255) aTTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAAGCAACGCATTCAGTACCGTg > 2:202490/1‑228 (MQ=255) aTTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAAGCAACGCATTCAGTACCGTg < 1:202490/228‑1 (MQ=255) gCTCGTCCACCGTGCCACCTGCGGTCATATGACCAAGCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAAGCAACGCATTCAGTACCGTGCCATc < 1:38599/229‑1 (MQ=255) gCTCGTCCACCCTGCCACCTGCGGTCATATGACCAATCGCCACCGCTTCATTCTCACGAGTAATTTCACGCCTTTTCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAAGCAACGCAt < 1:26854/213‑1 (MQ=255) tttCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAAGCAACGCATTc > 1:229491/1‑152 (MQ=255) aCGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAAGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAg > 1:36480/1‑179 (MQ=255) cGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAAGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATg < 2:36480/179‑1 (MQ=255) tCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATa > 1:244076/1‑100 (MQ=255) | CTGGTGCGTACGTCCAGTTTCCAGACGCAACCGCAGACGCGTGTGCACACGGAAGTGTTCCATGATGCGATAGTGAGTCACCGCTGGTTTGCCCATCGGATGCACCGCCATATGGGTACGTTTGGTCGGGTGGCGACTGATTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAATGAAGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATG > NC_000913/2735307‑2735699 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |