| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,173,361 | Δ2 bp | 100% | intergenic (‑488/+920) | gatD ← / ← gatB | galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,173,361 | 0 | C | . | 94.7% | 73.6 / ‑1.0 | 19 | intergenic (‑488/+921) | gatD/gatB | galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base . (10/8); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
| * | NC_000913 | 2,173,362 | 0 | C | . | 94.7% | 71.1 / ‑1.0 | 19 | intergenic (‑489/+920) | gatD/gatB | galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (10/8); minor base A (1/0); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCC > NC_000913/2173093‑2173585 || gCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGCTGATGCTCATAATGATGACACCCGAGCTTAACGTGCGGAACAGCTTTCCACGATGCACGCCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCCCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGCTaccaccgccga > 1:50918/1‑270 (MQ=255) tAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATg > 1:214216/1‑217 (MQ=255) acaGGGTATAGCTCAAAAGGTGGAAACCCAGGATTAAGGAGCGGAACAGATTTCCACGCTGCACGGCGACGGCCACCGCCACGAAGACGCCGCAGGTGGCAGGATAGCCAAACTGCATCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGCGGGATAAAAATCAGGCGTGCCGATACCA‑‑CCGCCGTATGTCCCATCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGGCCGCCGAACTTCGCCTGTAAACGACTACGAGc < 2:50918/274‑1 (MQ=255) tGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGACGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAACAATCAGTCTTGCCGATaccacc > 1:165422/1‑182 (MQ=255) tGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAACAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCATGACCGCCGACCTTCGCCTg > 1:185814/1‑250 (MQ=255) tGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCg > 1:125940/1‑275 (MQ=255) tCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGCCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt < 1:284594/274‑1 (MQ=255) atgaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCCGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGCCCACGGACCTGCTGAGCGAggggggggta > 2:284593/1‑269 (MQ=255) cccGAGATTACGGTGCGGAACAGATTTCCCGGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGACCGCCAAACGGCAGAACCTGATTACCCGCCACACAAACAGCAATTAAAATGGTGAGTGAGATAAAAATCAGGCTTGCCGATaccaccgccgta < 1:241974/171‑7 (MQ=255) ggggAACAGATTTCCACAATGCACGGCGACCGCCATCGCCCCGAAGAAGCCGATGGTGGCAAGACCGCCAAACGGCAGCACCTGATTACCCGGCAAACAAACAGCAATTAAAATTGTGAGTGGGATAAAAACCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTaa < 1:348281/273‑1 (MQ=255) ccACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGAGGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc < 1:346506/244‑1 (MQ=255) aagCCGATGGGGGAAAGACGGCCAAACTGAAGAACCTGATTACCCGGAAAACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACTA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGGTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTGTTTACCGCCAg < 2:125940/275‑1 (MQ=255) ggCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTAc > 1:365347/1‑139 (MQ=255) ggCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGACGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAAACCGCGAGGATGCCGATAAt > 1:20988/1‑275 (MQ=255) cacaAACATCAAATTAAATGGTGAGTGGGATAAAAATCAGGGGTGCCGAGACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGAGCAAGGGCGATCAGGAACTCCTGAGCGGCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatga < 1:1534/171‑1 (MQ=255) cacaAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc < 2:365346/139‑1 (MQ=255) aaaCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGCTCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCAGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTCATCACCCGTGGGATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGACGCAATACACATTTCACATCGTAACCCGCCAGCATGCCGATAACCAGccc > 1:80070/1‑274 (MQ=255) aaaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGCCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTACACGACTACGAGCCTGCTTAGCGATGGTGGTTAAACCATCCCTGATGGGTTTAATc > 1:241918/1‑167 (MQ=255) gATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAAc > 2:348846/1‑73 (MQ=255) aaaaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGATCTCCt < 1:348847/73‑1 (MQ=255) aGGCTTTCCGATA‑‑ACACCGCCGTATGTCCCAGCAGCAATGTCTGATCAATGCCTATCAGGAACTTCTATCTGCCGAACTTCCCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGtt > 2:321677/1‑120 (MQ=255) tGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTag > 1:129568/1‑153 (MQ=255) || GCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCC > NC_000913/2173093‑2173585 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |