| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,239,405 | Δ1 bp | 12.9% | coding (944/999 nt) | mglB ← | D‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,239,405 | 0 | C | . | 12.9% | 73.8 / 8.5 | 31 | coding (944/999 nt) | mglB | D‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base C (12/15); new base . (2/2); total (14/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.42e-01 | |||||||||||
GCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAATTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACACCGGAATGCTGGACTTGTTGTGTG > NC_000913/2239178‑2239609 | gCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCACCGGAGGACGGAGTCGTTGAGCTGACCGTATAATTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAAACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCACACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGt > 1:84077/1‑259 (MQ=255)gCCGGACTTTTAAACTAACGTTAGCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGGTCATTTCCAACAAGCATTCCCCGGAGGCCGGAGTCGTTGAGCTGACCATATAATTTTACCTTGTTGGCCATACAATAATGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTCCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGCCCATTTTGTTTTCGTTTTTTCCGTTGGTTCCATccccccc > 2:86059/1‑265 (MQ=255) gACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGCCCATATAATTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCAAGTTGGTGCCATCACCCGCCCCTTTAc > 1:349846/1‑275 (MQ=255) tACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATTATTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCa < 1:278154/275‑1 (MQ=255) aCCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAATTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCa < 1:86059/274‑1 (MQ=255) cTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATCATTTTACCTTGTTGGCCACACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATACGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGNTGCTTTCGCNtcggngtntccttg > 2:278153/1‑259 (MQ=255) tCATTACCAACAAGTATTTCCCGGAGGACGGAGTCGTTGAGCTGACCATATAATTTTACCGTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGGTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGtt < 1:88440/274‑1 (MQ=255) cGGAGGACGGAGTCGTTGAGCTGACCATATAATTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTTCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGCTTTTCGCCAGATCAAACGTCGCTTTCGCCTGGTTGTTAGCATCTTTCGGTCCGGTCCCCGcgcgggca > 1:107819/1‑268 (MQ=255) gTCGTTGAGCTGACCATATAAGTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAAGAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCGAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCa < 1:191848/237‑1 (MQ=255) gcAGTAATAGACTGCGCCCGATCAGTCATATTTCTTGCTGAATTCAGCCAGGTTGTCTGCATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGgttgt < 1:136812/182‑1 (MQ=255) gTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCa < 1:138556/184‑1 (MQ=255) tGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGgttgtt > 1:256990/1‑171 (MQ=255) cgcCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCAt > 2:175002/1‑174 (MQ=255) cgcCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCAt > 1:407959/1‑174 (MQ=255) cgcCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTGTGTTGTCGATTTTGCATTTGGTGGCCTCAGCCGCACCTTTCCCATCGGCCAGGTGTTTCGCCATATCAAAGGTCGCTTTCGCCTGGTCGTTAGCATCGTTc > 1:371551/1‑179 (MQ=255) cgcCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACG‑GGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCTCCAGATCAAAGGTTGCTTTCGCCTgg > 1:190111/1‑163 (MQ=255) cgcCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACG‑GGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTgg > 1:190119/1‑163 (MQ=255) cgcCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACACCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCTAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCCCCGGCTTTCCCCAGCGCCCGCGCTTCGTGCCGCGCAACGACTCCCAACACCGGACTGGCTGACTTGTtgtgtt > 2:125682/1‑272 (MQ=255) cgcCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGCTGTCTTTTTCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCAt > 1:63840/1‑105 (MQ=255) cccAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCGTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCa < 2:256989/171‑1 (MQ=255) gaaTCTGTTTTATTTCTTGCTGAATTCAGCCAGTTTGTCTTAACCTACGCCAACATAAGGTACGCGTACTGCTTTGTTGTCGATTTTCCAGTTATTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCgtc < 1:66610/135‑3 (MQ=255) gaaTCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTTGCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTCGGTGCCATCAGc < 2:63840/104‑1 (MQ=255) cAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGtt < 1:175003/174‑1 (MQ=255) cAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTATCGCCTGGTTGTTAGCATCNtt < 2:407958/174‑1 (MQ=255) cAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCAGCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACACCGGAATGCTg < 1:125682/256‑1 (MQ=255) cAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACG‑GGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGgttgt < 2:190118/163‑1 (MQ=255) aaaTCAGTCTTATTTCTTGCCGAATTCAGCCAGGTTGGCTTTATCTACGCCAACATAAGGTACG‑GGACCACTTTGTTGTCGATTTGCCAGTTGGGGCCATCAGCCGCACCCTTTTCATCGGCCACGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCAGgttgt < 2:190110/162‑1 (MQ=255) tCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAg < 1:86568/101‑1 (MQ=255) cGCAAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTTCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAACGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCCGCGCACGCGCTTCTGGCAGCGCATCGCCGCCAAACACCGGACTGCTGGACttg > 2:208199/1‑215 (MQ=255) aaCATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACACCGGAATGCTGGACTtgttgt < 1:208200/215‑1 (MQ=255) aaCATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCAACTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCa < 1:299096/118‑1 (MQ=255) | GCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAATTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACACCGGAATGCTGGACTTGTTGTGTG > NC_000913/2239178‑2239609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |