| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,000,980 | A→T | 13.9% | L26H (CTT→CAT) | yigE ← | DUF2233 domain‑containing protein YigE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,000,980 | 0 | A | T | 13.9% | 69.3 / 2.1 | 29 | L26H (CTT→CAT) | yigE | DUF2233 domain‑containing protein YigE |
| Reads supporting (aligned to +/- strand): ref base A (10/14); new base T (2/2); total (13/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
TGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGACACAC > NC_000913/4000744‑4001227 | tGTACAAACCGAGCGGCTCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCCCCTGACCCTGACTATTAATATCCGCCAGCAGTTCATGTAACCTTCCCCACGCTTCGACATTTGGTTGTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGAGTAACGGTATACGCCTGTACGGTCATCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAg < 1:393060/275‑1 (MQ=255) gaccctgacCCCTAATATCCGCCAGCAGCGCATGTAACCTTCACCACGCTTCGCCATTGGCTCTGTGCCAGCACATTTTCACCCGCTCCGTTAGCAGCTAACCGGTATTCGCCTGTACGGTCAGCGTCGCACCTGAACGTGCGCAATCATCTGCGGCAACAGCCAACAAGGGACCTAAGGTGAGGGCGCGACAACTCCGTCTGAGATACAAGGTGATCATTCCTTTACCAATGAGTAACTGATGCGACATGAGAGGTCCTGGGGGTGCGAgtttt > 2:73916/1‑275 (MQ=255) acTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGAtt > 1:70128/1‑201 (MQ=255) ttAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGATATTCAAg < 2:70128/201‑1 (MQ=255) tAATATCCGCCAGCAGAGCAGGTAACGTTCCCCACGCTTCGACATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGGGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTc < 1:20951/210‑1 (MQ=255) gCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAg < 2:173527/146‑1 (MQ=255) tCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGAtt > 2:156916/1‑168 (MQ=255) cACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAg < 1:156917/168‑1 (MQ=255) tCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGGTTGAGGATTAACGGTATACGCCTGTACGGGCAGCGTCGGATCTGGGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGGGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCACCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTCAACTa < 1:73916/275‑1 (MQ=255) aaCGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGATGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGa > 2:146464/1‑142 (MQ=255) aaCGGTATACGCCTGTACGGTCAGCGGCGGGTGTGGGAGTGCGCAATCATCCGCATCAACAGCAAACAACGGATGTAAGGGGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGAACATGCCTTTACCAATGAGTAGCTGa > 2:146453/1‑142 (MQ=255) gTATACGTCTGTACGGGCCGCGTCGGATCTTAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGATGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCCAGGTGATCATTCCTTTACCAATGAGTAGCTGATgcg < 1:146454/142‑1 (MQ=255) gTATACGCCTGTACGGGCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGATGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATgcg < 1:146465/142‑1 (MQ=255) cTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGCCAACAGCAAACAAGGGAAGTAAGGTGGGGGCGAGAAACATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTAAGGCGCCATCATAGATCCTGGATGAGGGCTTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAATTTCa > 1:143136/1‑209 (MQ=255) tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACACGGGAAGTAAGGTGAGGGCGAGAACAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTGGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTTTTGCCGACAAACTTTTGCTGGCGTAACATGCGCGCACGAt > 1:239788/1‑275 (MQ=255) tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACATTGACGAGTAACAAAAAGCGCGAAGTTCAACTAt > 1:165126/1‑211 (MQ=255) tACGGTAAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCTTGTACCAATGAGTAGCTGATGCGCCATTATAGGTCTTGGATGTGGGATTTTTTTATCCTGTTAGCGAACTTGACGAGTACCAAAAAACGCGAAGTTCa > 1:345051/1‑206 (MQ=255) aCGGTAGGCGTCGGATCCGGGGGTGCGCAATCATCAGCGGCAACAGCAAAGAAGAGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGGTGCGCCATTATAGGTCCTGGATGTGGGATTGTTTTATCCTTTTAGCGACCTTGACTAGTACCAAAAGGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGAt < 1:148096/274‑1 (MQ=255) gtaaGCGTCGGATCAGAGAGTGCGCAATAATCAGCGTGTACAGCAAACAAGTGAAGTAAGGTGAGGGCGAGNAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAACGAGTAGCTCATGAGCCATTATAGGTCCTGGATGTGGGATTTGTTTATCCTGTTATCGACCTAACCGGGTACAAAAAAGCNCGAATTTCAACTa < 2:345050/203‑1 (MQ=255) gTCAGCGTCGGATCTGAGAGTTCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGAGCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGAt < 1:189329/271‑1 (MQ=255) gTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTGACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGNCGGCAAAATTTTGCTGGCGTAACATGCGCGCACGAt < 1:150164/271‑1 (MQ=255) cGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGTAAAATCCGTTTGAGATGCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATGTTTGTAGCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAGTTTTg < 1:57148/243‑1 (MQ=255) cgcAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAgg > 1:170334/1‑72 (MQ=255) cgcAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTACTGGATGTGGGATTTTTTTATCCTGTTAGCGACCt > 1:107476/1‑152 (MQ=255) gcAATCATCAGCGGCAACAGCAAAAAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGGGATTGAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATTTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGac < 1:370063/275‑1 (MQ=255) atcatcAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGAc < 2:107476/152‑1 (MQ=255) agatggCGGCAAAAGCAAACAAATGAAGTAAGGTGGAGGCGGGAAAAATCCGGTTGGGATTCAAGGTTAGCATTCCTTTACCAAGTAGTAGCAGATCGGGCATTATAGGTACTGGATGTGGGATTTTTTTATCCTATACGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTGGACGGAAAAATTTTGCGGGCGTAATATGCGCGCACGATCACTCTAAGAGGACATTCTCCTTGGacacac < 2:64502/269‑1 (MQ=255) aCAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCt > 1:104100/1‑82 (MQ=255) aGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGTGCGAAGTTCACCTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGATATTCGCCTTGGac > 1:64502/1‑257 (MQ=255) | TGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTCAACTATTGTTCTGTGGTGTTCTGTTGCGTGTTGACGGCAAAATTTTGCTGGCGTAACATGCGCGCACGATCACTCTAAGAGGACATTCGCCTTGGACACAC > NC_000913/4000744‑4001227 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |