BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	1,464,573	Δ1 bp	coding (735/1314 nt)	paaK →	phenylacetyl‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	1,464,570	0	G	.	100.0%	515.0 / NA	98	coding (732/1314 nt)	paaK	phenylacetyl‑CoA ligase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (43/55); total (43/55)

Only 100 of 105 total aligned reads displayed.

GCTGAGCCGTGGACACAGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGATGGGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATCCTGAAATTGTTAATC  >  AC_000091/1464481‑1464655

gCTGAGCCGTGGACTCAGGACATGCGTAACGAGATTGAGCGTTGCCTGGGGATCACGGCACTGGATATTTATGGCCTGTCAGAAGTGATg                                                                                       <  2:310882/90‑1 (MQ=255)

gCTGAGCCGTGGACACAGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGATg                                                                                       >  2:402170/1‑90 (MQ=255)

 cTGAGCCGTGGACACAGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGATgg                                                                                      <  1:3173700/90‑1 (MQ=255)

  tGAGCCGTGGACACAGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGATggg                                                                                     >  1:1567528/1‑90 (MQ=255)

   gAGCCGTGGACACAGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGATgggc                                                                                    >  2:1846557/1‑89 (MQ=255)

    aGCCGTGGACACAGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGATgggcc                                                                                   >  1:429661/1‑88 (MQ=255)

    aGCCGTGGACACAGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGATgggcc                                                                                   >  2:2451474/1‑88 (MQ=255)

       cGTGGACACAGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCggg                                                                               <  1:657580/90‑1 (MQ=255)

       cGTGGACACAGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCggg                                                                               >  2:255875/1‑90 (MQ=255)

       cGTGGACACAGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCggg                                                                               >  1:536681/1‑90 (MQ=255)

        gTGGACACAGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCgggg                                                                              <  1:1437393/90‑1 (MQ=255)

          ggACACAGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGt                                                                            >  2:1035663/1‑90 (MQ=255)

            acacAGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTgg                                                                          >  2:2357797/1‑90 (MQ=255)

             cacaGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGc                                                                         <  1:1415227/90‑1 (MQ=255)

              acaGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGgcggcg                                                                        >  1:2461014/1‑90 (MQ=255)

               caGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGa                                                                       <  1:720880/90‑1 (MQ=255)

               caGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGa                                                                       <  1:533083/90‑1 (MQ=255)

                aGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGAt                                                                      <  1:1190490/90‑1 (MQ=255)

                aGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGAt                                                                      >  2:180724/1‑90 (MQ=255)

                aGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGAGGCGAt                                                                      >  1:2680930/1‑90 (MQ=255)

                     aTGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGGACTGGATATTTATGGCCTGTCAGAAATGAT‑GGGCCGGGGGTGGCGATGGAgt                                                                 >  1:63855/1‑90 (MQ=255)

                       gCGTAAAGAGATTGAGCGTCGCCTTGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAgtgt                                                               <  1:2636053/90‑1 (MQ=255)

                       gCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAgtgt                                                               <  2:3034715/90‑1 (MQ=255)

                         gTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCt                                                             >  1:1479919/1‑90 (MQ=255)

                           aaaGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTgg                                                           >  1:1122840/1‑90 (MQ=255)

                           aaaGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTgg                                                           >  2:1046509/1‑90 (MQ=255)

                            aaGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGa                                                          >  2:1737833/1‑90 (MQ=255)

                               agaTTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAAc                                                       >  2:1389118/1‑90 (MQ=255)

                                 aTTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTa                                                     <  1:399092/90‑1 (MQ=255)

                                 aTTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGCGTCTGGAAACTa                                                     >  2:2177401/1‑90 (MQ=255)

                                   tGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTAcc                                                   <  2:2780634/90‑1 (MQ=255)

                                      gCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGAc                                                <  2:1282580/90‑1 (MQ=255)

                                      gCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGAc                                                <  2:2241208/90‑1 (MQ=255)

                                       cGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACg                                               <  2:201990/90‑1 (MQ=255)

                                        gTCGCCTGGGGATCACCGCTCTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACgg                                              <  1:2992726/90‑1 (MQ=255)

                                          cGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGcc                                            >  1:305773/1‑90 (MQ=255)

                                          cGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGcc                                            >  2:3294674/1‑90 (MQ=255)

                                           gCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGccc                                           >  1:40832/1‑90 (MQ=255)

                                           gCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGccc                                           <  1:906131/90‑1 (MQ=255)

                                            ccTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCa                                          <  1:3438706/90‑1 (MQ=255)

                                            ccTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCa                                          <  1:2460888/90‑1 (MQ=255)

                                            ccTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCa                                          >  2:3170006/1‑90 (MQ=255)

                                              tGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAAc                                        <  2:940200/90‑1 (MQ=255)

                                              tGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAAc                                        <  1:3460593/90‑1 (MQ=255)

                                                gggATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATTGAGTGTCTGGAAACTACCGACGGCCCAACCa                                      >  1:1848789/1‑90 (MQ=255)

                                                gggATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCa                                      >  1:488232/1‑90 (MQ=255)

                                                gggATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCa                                      >  1:1170232/1‑90 (MQ=255)

                                                 ggATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCAt                                     >  1:435700/1‑90 (MQ=255)

                                                   aTCACCGCACTTGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCAAACCAttt                                   <  2:2837729/90‑1 (MQ=255)

                                                   aTCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCAttt                                   <  1:966252/90‑1 (MQ=255)

                                                   aTCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCAttt                                   <  1:1197989/90‑1 (MQ=255)

                                                   aTCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCAttt                                   <  2:3235886/90‑1 (MQ=255)

                                                    tCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTg                                  >  1:1803546/1‑90 (MQ=255)

                                                    tCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTg                                  >  2:1089475/1‑90 (MQ=255)

                                                    tCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTg                                  >  2:209972/1‑90 (MQ=255)

                                                    tCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTg                                  >  2:1567742/1‑90 (MQ=255)

                                                    tCACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTg                                  >  2:329152/1‑90 (MQ=255)

                                                     cACCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCCACCATTTgg                                 >  1:855766/1‑90 (MQ=255)

                                                      aCCGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTggg                                <  2:164369/90‑1 (MQ=255)

                                                       ccGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGa                               <  2:1623038/90‑1 (MQ=255)

                                                        cGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGaa                              <  1:908011/90‑1 (MQ=255)

                                                        cGCACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGaa                              <  2:3167822/90‑1 (MQ=255)

                                                          cACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGa                            <  1:3112747/90‑1 (MQ=255)

                                                          cACTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGa                            <  1:459611/90‑1 (MQ=255)

                                                           aCTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGGCCAACCATTTGGGAAGAt                           >  1:2399573/1‑90 (MQ=255)

                                                           aCTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGAt                           >  2:32920/1‑90 (MQ=255)

                                                           aCTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGAt                           <  2:2055668/90‑1 (MQ=255)

                                                           aCTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGAt                           <  2:2583632/90‑1 (MQ=255)

                                                            cTGGATGTTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGAtg                          >  2:3477288/1‑89 (MQ=255)

                                                            cTGGATATTTATGTCCTGTCAGAAGTGAT‑GGGCCGGGGTTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATc                          <  1:333602/90‑1 (MQ=255)

                                                            cTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATc                          >  2:563464/1‑90 (MQ=255)

                                                            cTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATc                          <  2:689461/90‑1 (MQ=255)

                                                            cTGGATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGAGGCGATGGAGTGGCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATc                          >  2:2902920/1‑90 (MQ=255)

                                                              ggATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCAt                        <  1:1108511/90‑1 (MQ=255)

                                                              ggATATTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGGGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCAt                        >  1:1485185/1‑90 (MQ=255)

                                                                atatTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCAttt                      <  2:2314369/90‑1 (MQ=255)

                                                                atatTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCAttt                      <  1:2018719/90‑1 (MQ=255)

                                                                 tatTTATGGCCTGTCAGAAGTGAT‑GGGCCGGGGTTGGCGAGGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTc                     <  2:2450439/90‑1 (MQ=255)

                                                                   tttATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTa                   >  2:605419/1‑90 (MQ=255)

                                                                   tttATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTa                   <  1:2058412/90‑1 (MQ=255)

                                                                   tttATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTa                   <  2:3424032/90‑1 (MQ=255)

                                                                    ttATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTAt                  >  2:43212/1‑90 (MQ=255)

                                                                     tATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATc                 <  2:3258466/90‑1 (MQ=255)

                                                                     tATGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATc                 <  1:3126169/90‑1 (MQ=255)

                                                                      aTGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATcc                <  1:2055045/90‑1 (MQ=255)

                                                                       tGGCCTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATCCt               >  1:353552/1‑90 (MQ=255)

                                                                           cTGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATCCTGaaa           <  1:43212/90‑1 (MQ=255)

                                                                            tGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATCCTGAAAt          <  1:358624/90‑1 (MQ=255)

                                                                            tGTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATCCTGAAAt          >  1:3124067/1‑90 (MQ=255)

                                                                             gTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATCCTGAAAtt         <  2:1298902/90‑1 (MQ=255)

                                                                             gTCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATCCTGAAAtt         <  1:2479164/90‑1 (MQ=255)

                                                                              tCAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATCCTGAAATTg        <  2:800802/90‑1 (MQ=255)

                                                                               cAGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATCCTGAAATTGt       >  2:3164344/1‑90 (MQ=255)

                                                                                aGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATCCTGAAATTGtt      >  2:632569/1‑90 (MQ=255)

                                                                                aGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATCCTGAAATTGtt      <  1:1047171/90‑1 (MQ=255)

                                                                                aGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATCCTGAAATTGtt      <  1:624342/90‑1 (MQ=255)

                                                                                aGAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATCCTGAAACTGtt      <  2:2587447/90‑1 (MQ=255)

                                                                                 gAAGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATCCTGAAATTGTTa     <  2:2474460/90‑1 (MQ=255)

                                                                                  aaGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATCCTGAAATTGTTaa    <  2:1256345/90‑1 (MQ=255)

                                                                                  aaGTGAT‑GGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATCCTGAAATTGTTaa    <  2:1917001/90‑1 (MQ=255)

GCTGAGCCGTGGACACAGGCCATGCGTAAAGAGATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTGTCAGAAGTGATGGGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTATCCTGAAATTGTTAATC  >  AC_000091/1464481‑1464655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`