| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AC_000091 | 3,912,304 | (G)8→10 | coding (1261/1455 nt) | xylB → | xylulokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AC_000091 | 3,912,296 | 1 | . | G | 95.6% | 163.8 / 2.4 | 45 | coding (1253/1455 nt) | xylB | xylulokinase |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (34/9); total (34/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01 | |||||||||||
| * | AC_000091 | 3,912,296 | 2 | . | G | 82.2% | 140.3 / 30.9 | 45 | coding (1253/1455 nt) | xylB | xylulokinase |
| Reads supporting (aligned to +/- strand): ref base . (4/4); new base G (30/7); total (34/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.51e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
AGTGTTACGTTGATTGGGGGCGGGGCGCGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑AC‑‑‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATCCAGAGAAATCGCTCATTGAAT > AC_000091/3912208‑3912376 || aGTGTTACGTTGATTGGGGGCGGGGCGCGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑AC‑‑‑g > 1:1027450/1‑90 (MQ=255)aGTGTTACGATGATTGGGGGCGGGGCGCGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGG‑‑AC‑‑‑g > 2:2001457/1‑90 (MQ=255) ttACGTTGATTGGGGGCGGGGCGCGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑AC‑‑‑ggg‑‑gg < 1:1133411/90‑1 (MQ=255) ttACGTTGATTGGGGGCGGGGCGCGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑AC‑‑‑ggg‑‑gg < 1:1544427/90‑1 (MQ=255) tACGTTGATTGGGGGCGGGGCGCGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑AC‑‑‑ggg‑‑ggg > 2:2736502/1‑90 (MQ=255) aCGTTGATTGGGGGCGGGGCGCGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑AC‑‑‑ggg‑‑gggg > 2:3345207/1‑90 (MQ=255) aCGTTGATTGGGGGCGGGGCGCGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑AC‑‑‑ggg‑‑gggg > 2:3264051/1‑90 (MQ=255) aCGTTGATTGGGGGCGGGGCGCGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑AC‑‑‑ggg‑‑gggg < 2:1242052/90‑1 (MQ=255) cGTTGATTGGGGGCGGGGCGCGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑AC‑‑‑ggg‑‑ggggg > 1:3360916/1‑90 (MQ=255) cGTTGATTGGGGGCGGGGCGCGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑AC‑‑‑ggg‑‑ggggg > 2:1693407/1‑90 (MQ=255) gTTGATTGGGGGCGGGGCGCGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑AC‑‑‑ggg‑‑gggggg > 2:555778/1‑89 (MQ=255) tGATTGGGGGCGGGGCGCGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑AC‑‑‑ggg‑‑gggggggg > 1:2247940/1‑87 (MQ=255) tGATTGGGGGCGGGGCGCGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑AC‑‑‑ggg‑‑ggggggga < 2:3220283/90‑4 (MQ=255) gATTGGGGGCGGGGCGCGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑AC‑‑‑ggg‑‑ggggggggt > 2:1265173/1‑86 (MQ=255) cggggcgCGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGGGGGGCCa > 2:2366078/1‑90 (MQ=255) ggggcgCGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGGCAg > 2:1807728/1‑90 (MQ=255) ggggcgCGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACG‑‑GGG‑‑GGGGGATGTGGGGCCAGc > 1:966595/1‑90 (MQ=255) gcgcGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGGGGGGCCCGCAc > 2:3329704/1‑90 (MQ=255) cgcgTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACG‑‑GGG‑‑GGGGGATGTGGGGCCAGCACTg > 1:3153/1‑90 (MQ=255) cgTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTgg > 1:1079373/1‑90 (MQ=255) gTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGGTGTGGGGCCAGCCCgggg > 1:2088209/1‑90 (MQ=255) tAGGGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGA‑‑ACG‑‑GGG‑‑GGGGGATGTGGTGCCAGCACTGGgcg > 1:727582/1‑90 (MQ=255) aGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCCCTGGgcg > 1:2978450/1‑90 (MQ=255) aGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGgcg > 1:2935777/1‑90 (MQ=255) aGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGgcg > 2:368097/1‑90 (MQ=255) gTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGGTGTGGGGCCACCACTGGgcgc > 1:2773717/1‑90 (MQ=255) tACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCaa > 1:3033129/1‑90 (MQ=255) tACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACG‑‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAg > 1:1052472/1‑90 (MQ=255) aCTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑AC‑‑‑GGGGTGGGGGATGTGGGGCCAGCACTGGGCGCAGCAAg < 2:1129706/90‑1 (MQ=255) gCGTCAGATGCTGGCGGATATCAGCGGGCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTg > 1:1120095/1‑90 (MQ=255) cGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCCCTGGGGGCAGCAAGGCTgg > 2:1667442/1‑90 (MQ=255) cGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTgg > 2:3052329/1‑90 (MQ=255) aGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGGGGGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGgcgc > 1:1992777/1‑90 (MQ=255) aGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCCCTGGGCGCAGCAAGGCTGGCGCa > 2:2941113/1‑90 (MQ=255) gATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAg > 1:3164085/1‑90 (MQ=255) ttggtGGGGGATAACAGGGGTCAGCTGCGGGATTTCCGTGGGG‑‑‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGa < 2:3033644/86‑1 (MQ=255) gCTGGCGGATAGCAGGGGTAAGCAGCTCGATTCCCGT‑‑CCGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATc < 1:2318127/90‑1 (MQ=255) ggCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGGGCAGATcgcg > 1:3065447/1‑90 (MQ=255) gCGGATATCGGCGGCCAGCAGCTCGATTCCCGT‑‑GCG‑‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCgcggc < 1:161556/90‑1 (MQ=255) gCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACG‑‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCgcggc < 2:1421603/90‑1 (MQ=255) ggATATCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCgcggcg > 1:631781/1‑90 (MQ=255) catgtCAGCGGTCCGCAGCTCGATTTCCGT‑‑CCGG‑GGG‑‑GGGGGAGGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGa < 2:1691572/86‑1 (MQ=255) atatCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGGTGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGGGCAGAACGGGGCGaa > 2:369434/1‑90 (MQ=255) atatCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGaa > 1:86250/1‑90 (MQ=255) atCAGCGGTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGGTGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATc > 1:1491286/1‑90 (MQ=255) tCAGCGGTCAGCAGCTGGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATcc < 2:2347514/90‑1 (MQ=255) ccAGCGGTCAGCGGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATcc < 2:3424629/89‑1 (MQ=255) gCGGTCAGGAGTTCGATTGCCGG‑‑GCGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATCCaga < 1:183400/90‑1 (MQ=255) cGGTCAGCAGCTCGATTCCCGT‑‑GCGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATCCagag < 1:1212330/90‑1 (MQ=255) ggTCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATCCAgaga > 1:3132632/1‑90 (MQ=255) ggTCAGCAGCTCGATTACCGT‑‑ACG‑‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATCCAGAGaa > 1:1045414/1‑90 (MQ=255) ggTCAGCAGCCCGATTCCCGT‑‑GCGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATCCAgaga < 1:527234/90‑1 (MQ=255) tCAGCAGCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATCCAGAGaaa > 1:2058251/1‑90 (MQ=255) cagcagCTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATCCAGAGAAAt > 2:3267444/1‑90 (MQ=255) gcagcTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATCCAGAGAAATCg < 2:2529101/90‑1 (MQ=255) agcTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATCCAGAGAAATCGCt > 2:1367405/1‑90 (MQ=255) agcTCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATCCAGAGAAATCGCt > 1:2553579/1‑90 (MQ=255) tCGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATCCAGAGAAATCGCTCAt > 1:1859607/1‑90 (MQ=255) cGATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATCCAGAGAAATCGCTCAtt > 2:1103684/1‑90 (MQ=255) gATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATCCAGAGAAATCGCTCATTg > 1:374193/1‑90 (MQ=255) gATTACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATCCAGAGAAATCGCTCATTg > 1:2118409/1‑90 (MQ=255) ttACCGT‑‑ACGG‑GGG‑‑GGGGGGTGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATCCAGGGAAATCGCTCATTGaa > 2:1211640/1‑90 (MQ=255) ttACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATCCAAAGAAAACGCTCATTaaa > 2:29164/1‑90 (MQ=255) tACCGT‑‑ACGG‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATCCAGAGAAATCGCTCATTGAAt > 2:719541/1‑90 (MQ=255) || AGTGTTACGTTGATTGGGGGCGGGGCGCGTAGTGAGTACTGGCGTCAGATGCTGGCGGATATCAGCGGTCAGCAGCTCGATTACCGT‑‑AC‑‑‑GGG‑‑GGGGGATGTGGGGCCAGCACTGGGCGCAGCAAGGCTGGCGCAGATCGCGGCGAATCCAGAGAAATCGCTCATTGAAT > AC_000091/3912208‑3912376 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |