BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	251,759	(G)_5→6	coding (862/1056 nt)	dinB →	DNA polymerase IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	251,754	1	.	G	98.2%	441.7 / 1.0	113	coding (857/1056 nt)	dinB	DNA polymerase IV
Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (62/49); total (63/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

GTCTGAATGTGAAGCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑A‑GGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGC  >  AC_000091/251665‑251838

gTCTGAATGTGAAGCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑a                                                                                        <  1:3351171/90‑1 (MQ=255)

gTCTGAATGTGAAGCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑a                                                                                        <  2:2813241/90‑1 (MQ=255)

 tCTGAATGTGAAGCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑A‑g                                                                                      <  2:2130164/90‑1 (MQ=255)

 tCTGAATGTGAAGCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑A‑g                                                                                      <  1:161700/90‑1 (MQ=255)

  cTGAATGTGAAGCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑A‑gg                                                                                     >  1:3442555/1‑90 (MQ=255)

   tGAATGTGAAGCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑A‑ggg                                                                                    >  1:250922/1‑90 (MQ=255)

   tGAATGTGAAGCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑A‑ggg                                                                                    >  1:3092629/1‑90 (MQ=255)

   tGAATGTGAAGCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑A‑ggg                                                                                    <  1:1843201/90‑1 (MQ=255)

    gAATGTGAAGCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑A‑ggg‑g                                                                                  <  1:888950/90‑1 (MQ=255)

      aTGTGAAGCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑A‑ggg‑ggg                                                                                <  2:341034/90‑2 (MQ=255)

      aTGTGAAGCGATTATCGAGCGGCTGTATCCGGAACTTGAACACCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑A‑ggg‑ggg                                                                                >  2:1216527/1‑89 (MQ=255)

        gtgAAGCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑A‑ggg‑gggtg                                                                              >  1:2833107/1‑87 (MQ=255)

         tgAAGCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑A‑ggg‑gggtga                                                                             >  2:2196410/1‑86 (MQ=255)

         tgAAGCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑A‑ggg‑gggtga                                                                             <  1:320867/90‑5 (MQ=255)

             gCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAAtt                                                                          <  2:1048831/90‑1 (MQ=255)

              cGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTa                                                                         <  2:2048308/90‑1 (MQ=255)

              cGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTa                                                                         >  1:1937093/1‑90 (MQ=255)

              cGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAAACTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTa                                                                         >  2:2532907/1‑90 (MQ=255)

               gATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTaa                                                                        >  2:3142978/1‑90 (MQ=255)

               gATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTaa                                                                        <  1:2673632/90‑1 (MQ=255)

               gATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTaa                                                                        >  2:910029/1‑90 (MQ=255)

               gATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTaa                                                                        >  1:1166145/1‑90 (MQ=255)

                aTTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTaaa                                                                       >  1:231599/1‑90 (MQ=255)

                 ttATCGAGCTGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAg                                                                      <  2:693408/90‑1 (MQ=255)

                  tATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGt                                                                     >  1:805774/1‑90 (MQ=255)

                    tCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTc                                                                   >  1:329832/1‑90 (MQ=255)

                     cGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCGG‑GGG‑GGTGAAATTAAAGTTcg                                                                  >  1:142849/1‑90 (MQ=255)

                     cGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTcg                                                                  <  1:163647/90‑1 (MQ=255)

                      gAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTcga                                                                 >  2:2680463/1‑90 (MQ=255)

                      gAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGCAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTcga                                                                 >  1:1956328/1‑90 (MQ=255)

                       aGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTcgac                                                                >  2:2903327/1‑90 (MQ=255)

                         cGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTcgacga                                                              <  1:2099370/90‑1 (MQ=255)

                          ggCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGAt                                                             <  1:1268765/90‑1 (MQ=255)

                          ggCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGAt                                                             >  2:4989/1‑90 (MQ=255)

                          ggCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGAt                                                             >  1:363103/1‑90 (MQ=255)

                           gCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGAtt                                                            <  1:2731764/90‑1 (MQ=255)

                           gCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGAtt                                                            <  1:1635370/90‑1 (MQ=255)

                            cTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGAttt                                                           >  1:3353753/1‑90 (MQ=255)

                             tGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGAtttt                                                          <  2:1579391/90‑1 (MQ=255)

                             tGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGAtttt                                                          <  1:430515/90‑1 (MQ=255)

                              gTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTc                                                         <  2:583399/90‑1 (MQ=255)

                              gTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTc                                                         <  1:743758/90‑1 (MQ=255)

                               tATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCa                                                        >  2:870860/1‑90 (MQ=255)

                                  ccGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCa                                                     >  2:3105934/1‑90 (MQ=255)

                                  ccGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCa                                                     <  2:1151920/90‑1 (MQ=255)

                                   cGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCaa                                                    >  1:1552145/1‑90 (MQ=255)

                                    ggAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCaaa                                                   <  2:2459993/90‑1 (MQ=255)

                                    ggAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCaaa                                                   >  2:885429/1‑90 (MQ=255)

                                    ggAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCaaa                                                   >  1:1490082/1‑90 (MQ=255)

                                     gAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGGTTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAac                                                  >  2:1472447/1‑90 (MQ=255)

                                        cTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAaccac                                               <  1:510732/90‑1 (MQ=255)

                                        cTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAaccac                                               <  2:242309/90‑1 (MQ=255)

                                          tGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCAccc                                             >  1:538775/1‑90 (MQ=255)

                                           gAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCa                                            <  1:1314609/90‑1 (MQ=255)

                                             aCGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAgg                                          <  1:2787043/90‑1 (MQ=255)

                                             aCGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAgg                                          <  2:1947454/90‑1 (MQ=255)

                                             aCGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑A‑GGGTGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAgg                                          <  1:1230790/90‑1 (MQ=255)

                                              cGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGa                                         <  2:3260275/90‑1 (MQ=255)

                                               gCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAg                                        >  1:924989/1‑90 (MQ=255)

                                               gCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAg                                        <  1:586737/90‑1 (MQ=255)

                                                 cGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCa                                      >  1:2289677/1‑90 (MQ=255)

                                                 cGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCa                                      <  2:2505193/90‑1 (MQ=255)

                                                 cGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCa                                      >  2:1414342/1‑90 (MQ=255)

                                                 cGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCa                                      <  1:215778/90‑1 (MQ=255)

                                                  gTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCAc                                     >  1:3084397/1‑90 (MQ=255)

                                                  gTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCAc                                     <  2:2239214/90‑1 (MQ=255)

                                                   tCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACg                                    >  1:2790046/1‑90 (MQ=255)

                                                     tGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTc                                  >  2:2693014/1‑90 (MQ=255)

                                                       gCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTg                                >  2:502196/1‑90 (MQ=255)

                                                         aaaGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGc                              >  2:3073205/1‑90 (MQ=255)

                                                          aaGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGcc                             <  2:352911/90‑1 (MQ=255)

                                                           aGGTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCcg                            >  1:698054/1‑90 (MQ=255)

                                                             gTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCcgcg                          >  1:1195193/1‑90 (MQ=255)

                                                             gTAAAACCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCcgcg                          <  1:827196/90‑1 (MQ=255)

                                                               aaaaCCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGc                        <  2:1641540/90‑1 (MQ=255)

                                                               aaaaCCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGc                        >  2:185525/1‑90 (MQ=255)

                                                                  aCCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTTAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGa                     >  2:1542987/1‑90 (MQ=255)

                                                                  aCCTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGa                     <  2:1531005/90‑1 (MQ=255)

                                                                   ccTGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGaa                    <  2:3212544/90‑1 (MQ=255)

                                                                     tGATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATa                  <  2:2790157/90‑1 (MQ=255)

                                                                      gATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATaa                 >  2:2165589/1‑90 (MQ=255)

                                                                      gATTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATaa                 <  2:216179/90‑1 (MQ=255)

                                                                       aTTTACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATaaa                >  1:1131978/1‑90 (MQ=255)

                                                                        tttACTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAg               <  2:2471854/90‑1 (MQ=255)

                                                                            cTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGa           >  2:2102287/1‑90 (MQ=255)

                                                                            cTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGa           >  1:3266432/1‑90 (MQ=255)

                                                                            cTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGa           >  1:862905/1‑90 (MQ=255)

                                                                            cTGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGa           >  2:1957159/1‑90 (MQ=255)

                                                                             tGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGAt          <  2:415934/90‑1 (MQ=255)

                                                                             tGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGAt          >  1:321718/1‑90 (MQ=255)

                                                                             tGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGAt          >  1:435156/1‑90 (MQ=255)

                                                                             tGATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGAt          >  1:1389368/1‑90 (MQ=255)

                                                                              gATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGATc         >  2:1129703/1‑90 (MQ=255)

                                                                              gATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGATc         >  2:1040658/1‑90 (MQ=255)

                                                                              gATTGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGATc         >  1:3300360/1‑90 (MQ=255)

                                                                                ttGCTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTa       <  2:1332037/90‑1 (MQ=255)

                                                                                   cTCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATc    >  2:1455350/1‑90 (MQ=255)

                                                                                    tCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCg   <  2:1219755/90‑1 (MQ=255)

                                                                                    tCGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCg   <  2:1851125/90‑1 (MQ=255)

                                                                                     cGCC‑AGGGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTCATCGc  >  2:891783/1‑90 (MQ=255)

GTCTGAATGTGAAGCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCC‑A‑GGG‑GGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGC  >  AC_000091/251665‑251838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`