Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	1,072,535	(G)5→6	coding (47/387 nt)	ycdK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	1,072,530	1	.	G	100.0%	310.6 / NA	80	F18L (TTC→CTC)	ycdK	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (41/39);  total (41/39)

GTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAA‑‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGA  >  AC_000091/1072441‑1072613                                                                                           |                                                                                     gTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGaa                                                                                       >  2:2012956/1‑90 (MQ=255) gTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGaa                                                                                       <  1:1910364/90‑1 (MQ=255)  tCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAA‑‑g                                                                                    <  2:2962034/90‑1 (MQ=255)   cATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAA‑‑gg                                                                                   >  1:2087799/1‑90 (MQ=255)   cATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAA‑‑gg                                                                                   <  2:2517817/90‑1 (MQ=255)    aTCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAA‑‑ggg                                                                                  >  2:2294482/1‑90 (MQ=255)      cGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAA‑‑ggggg                                                                                >  1:3388216/1‑90 (MQ=255)         cAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAA‑‑ggggggcc                                                                             <  2:3449278/90‑4 (MQ=255)         cAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAA‑‑ggggggcc                                                                             <  2:2796904/90‑4 (MQ=255)          aaaCAGCACGTTATTATGTCGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAA‑‑ggggggcca                                                                            <  2:3395919/90‑5 (MQ=255)            aCAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGc                                                                           >  1:323972/1‑90 (MQ=255)             cAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCg                                                                          >  2:2828009/1‑90 (MQ=255)             cAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCg                                                                          >  1:1754572/1‑90 (MQ=255)             cAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCg                                                                          >  1:1648871/1‑90 (MQ=255)              aGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCgg                                                                         >  2:736048/1‑90 (MQ=255)              aGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCgg                                                                         >  2:1792829/1‑90 (MQ=255)               gCACGTTATTATGTTGATCAAAATCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGt                                                                        <  2:2088853/90‑1 (MQ=255)               gCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGt                                                                        >  2:3075030/1‑90 (MQ=255)                 aCGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTgc                                                                      <  2:1297841/90‑1 (MQ=255)                 aCGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTgc                                                                      >  2:764079/1‑90 (MQ=255)                 aCGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTgc                                                                      <  1:1111511/90‑1 (MQ=255)                 aCGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGCGCCGGGAACGAAG‑GGGGGCCAGCGGTgc                                                                      >  2:598194/1‑90 (MQ=255)                  cGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTgcg                                                                     >  1:2135635/1‑90 (MQ=255)                   gTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTgcgc                                                                    >  2:3342132/1‑90 (MQ=255)                    ttattaTGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCgct                                                                   <  2:1371214/90‑1 (MQ=255)                    ttattaTGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCgct                                                                   >  1:2807105/1‑90 (MQ=255)                    tgattatGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCgct                                                                   <  2:2111436/88‑1 (MQ=255)                       ttatGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCgctgct                                                                <  1:1282752/90‑1 (MQ=255)                          tGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGcc                                                             <  1:314840/90‑1 (MQ=255)                          tGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGcc                                                             <  1:2583504/90‑1 (MQ=255)                          tGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGcc                                                             >  2:206984/1‑90 (MQ=255)                           gTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCa                                                            <  1:3425980/90‑1 (MQ=255)                            ttGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAg                                                           >  1:1225407/1‑90 (MQ=255)                            ttGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAg                                                           <  1:1976246/90‑1 (MQ=255)                              gATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCg                                                         <  2:2359529/90‑1 (MQ=255)                              gATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCg                                                         >  1:2315171/1‑90 (MQ=255)                                 cAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGa                                                      <  1:2375596/90‑1 (MQ=255)                                  aaaaGCCAGCGTACCGGAGACATACACCGCGCCATCAGCCAGCGTGCCGGCAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGaa                                                     <  2:3384463/90‑1 (MQ=255)                                    aaGCCAGCGTACCGGAGACATACACCCCGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCCGCTGCCAGCGGGaata                                                   <  1:1841095/90‑1 (MQ=255)                                    aaGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGaata                                                   >  2:2174444/1‑90 (MQ=255)                                    aaGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGaata                                                   >  1:1970771/1‑90 (MQ=255)                                     aGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGaataa                                                  >  1:1058947/1‑90 (MQ=255)                                      gCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGaataat                                                 <  1:494830/90‑1 (MQ=255)                                       ccAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAAtt                                                >  2:2080835/1‑90 (MQ=255)                                        cAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTa                                               >  2:231457/1‑90 (MQ=255)                                         aGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTAc                                              >  1:2011614/1‑90 (MQ=255)                                         aGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTAc                                              <  1:2236729/90‑1 (MQ=255)                                         aGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTAc                                              >  1:1478694/1‑90 (MQ=255)                                         aGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTAc                                              <  1:542084/90‑1 (MQ=255)                                           cGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACgg                                            >  2:2582922/1‑90 (MQ=255)                                           cGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACgg                                            >  1:1334895/1‑90 (MQ=255)                                             tACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGAt                                          <  1:3371053/90‑1 (MQ=255)                                              aCCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGAtt                                         >  2:3342660/1‑90 (MQ=255)                                              aCCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGAtt                                         >  1:434668/1‑90 (MQ=255)                                               ccGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGAtt                                         <  1:1743676/90‑1 (MQ=255)                                               ccGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGAttt                                        <  1:2895373/90‑1 (MQ=255)                                                cGGAGACATGCCCCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGAtttt                                       <  2:2640491/90‑1 (MQ=255)                                                  gagaCATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTgg                                     <  2:1215626/90‑1 (MQ=255)                                                     aCATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCAt                                  <  1:809416/90‑1 (MQ=255)                                                      cATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATc                                 >  2:1961227/1‑90 (MQ=255)                                                      cATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATc                                 >  1:1713868/1‑90 (MQ=255)                                                       aTACACCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCg                                <  2:769396/90‑1 (MQ=255)                                                         acacCACGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGtt                              >  2:453852/1‑90 (MQ=255)                                                          caccacGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTa                             <  2:3394406/90‑1 (MQ=255)                                                          caccacGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTa                             >  2:1765993/1‑90 (MQ=255)                                                          caccacGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTa                             >  2:2120939/1‑90 (MQ=255)                                                            ccacGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTaaa                           <  2:3281073/90‑1 (MQ=255)                                                              acGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACt                         >  2:1838655/1‑90 (MQ=255)                                                               cGCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTc                        >  2:2024094/1‑90 (MQ=255)                                                                gCCATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTcc                       >  1:2046190/1‑90 (MQ=255)                                                                  cATCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCtt                     >  2:2130434/1‑90 (MQ=255)                                                                   aTCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTa                    >  1:161654/1‑90 (MQ=255)                                                                    tCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTaa                   <  2:2683953/90‑1 (MQ=255)                                                                    tCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTaa                   <  2:2282020/90‑1 (MQ=255)                                                                    tCAGCCAGCGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTaa                   <  1:925550/90‑1 (MQ=255)                                                                     cagccagcGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAg                  >  2:1706189/1‑90 (MQ=255)                                                                     cagccagcGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAg                  <  2:3170229/90‑1 (MQ=255)                                                                      agccagcGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGc                 <  2:1327497/90‑1 (MQ=255)                                                                      agccagcGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGc                 >  1:735954/1‑90 (MQ=255)                                                                         cagcGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGat              >  1:398714/1‑90 (MQ=255)                                                                          agcGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGata             >  2:1190583/1‑90 (MQ=255)                                                                           gcGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGatat            <  2:1800172/90‑1 (MQ=255)                                                                            cGTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATg           <  1:827748/90‑1 (MQ=255)                                                                             gTGCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGa          <  2:1753903/90‑1 (MQ=255)                                                                               gCCGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGc        <  2:2337553/90‑1 (MQ=255)                                                                                ccGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCa       >  1:2428210/1‑90 (MQ=255)                                                                                 cGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa      <  2:2275603/90‑1 (MQ=255)                                                                                 cGGGAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa      >  2:1277233/1‑90 (MQ=255)                                                                                  gggAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaaa     <  2:1000411/90‑1 (MQ=255)                                                                                  gggAACGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaaa     <  2:666938/90‑1 (MQ=255)                                                                                     aaCGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGa  <  1:1683749/90‑1 (MQ=255)                                                                                     aaCGAAG‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGa  <  2:1966676/90‑1 (MQ=255)                                                                                           |                                                                                     GTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAA‑‑GGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGA  >  AC_000091/1072441‑1072613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑