BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	1,139,752	(G)_5→6	coding (805/942 nt)	flgJ →	muramidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	1,139,747	1	.	G	100.0%	419.5 / NA	107	Q267R (CAG→CGG)	flgJ	muramidase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (51/56); total (51/56)

Only 100 of 122 total aligned reads displayed.

TCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACA‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAA  >  AC_000091/1139659‑1139831

 cGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACA‑gg                                                                                    >  1:2681524/1‑90 (MQ=255)

 cGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACA‑gg                                                                                    >  2:1355500/1‑90 (MQ=255)

 cGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACAGCCGCGAGTGCGGAACA‑gg                                                                                    >  1:1031164/1‑90 (MQ=255)

  gTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACA‑ggg                                                                                   <  2:208758/90‑1 (MQ=255)

   tATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACA‑gggg                                                                                  >  2:1705795/1‑90 (MQ=255)

   tATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACA‑gggg                                                                                  <  2:1824111/90‑1 (MQ=255)

   tATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACA‑gggg                                                                                  <  1:2060131/90‑1 (MQ=255)

   tATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCAGCGAGGGCGGAACA‑gggg                                                                                  >  2:712078/1‑90 (MQ=255)

    aTCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACA‑ggggg                                                                                 <  1:3369335/90‑1 (MQ=255)

    aTCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACA‑ggggg                                                                                 >  1:2273685/1‑90 (MQ=255)

     tCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACA‑gggggg                                                                                <  2:2626015/90‑2 (MQ=255)

       tGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACA‑ggggggcg                                                                              >  2:2440090/1‑87 (MQ=255)

          aaGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAg                                                                            >  2:3128022/1‑90 (MQ=255)

          aaGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAg                                                                            >  2:2735381/1‑90 (MQ=255)

           aGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAgg                                                                           >  2:2441231/1‑90 (MQ=255)

           aGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAgg                                                                           <  1:3264644/90‑1 (MQ=255)

              cTTGTCGGATTACGTTGGTCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGccc                                                                        >  2:343570/1‑90 (MQ=255)

              cTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGccc                                                                        >  1:1383943/1‑90 (MQ=255)

              cTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGccc                                                                        >  1:2550522/1‑90 (MQ=255)

               ttGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCt                                                                       <  1:1473889/90‑1 (MQ=255)

               ttGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGAGCAGGCCCt                                                                       <  2:2309410/90‑1 (MQ=255)

               ttGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGGGCGGAACAGGGGGGCGCAGGACCt                                                                       >  1:3216578/1‑90 (MQ=255)

                tGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTa                                                                      >  1:1234229/1‑90 (MQ=255)

                tGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACCCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTa                                                                      <  2:2259655/90‑1 (MQ=255)

                 gTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTAc                                                                     <  2:1970259/90‑1 (MQ=255)

                 gTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTAc                                                                     >  2:256822/1‑90 (MQ=255)

                 gTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTAc                                                                     <  2:1399983/90‑1 (MQ=255)

                 gTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTAc                                                                     >  1:1952792/1‑90 (MQ=255)

                 gTCGGATTACGTTGGGCTGTTAACGCGGAACCCGCGCTACGCCGCCGTGACGGCCGCCGCGAGTGCGGAATAGGGGGGCGCAGGCCCAAc                                                                     >  2:1198500/1‑90 (MQ=255)

                  tCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACa                                                                    <  2:3394991/90‑1 (MQ=255)

                  tCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACa                                                                    >  1:1297205/1‑90 (MQ=255)

                   cGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAg                                                                   <  1:2844247/90‑1 (MQ=255)

                   cGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAg                                                                   >  2:2215013/1‑90 (MQ=255)

                   cGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAg                                                                   >  1:1425111/1‑90 (MQ=255)

                     gATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGa                                                                 >  2:3245080/1‑90 (MQ=255)

                      aTTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGAc                                                                >  2:2449166/1‑90 (MQ=255)

                       ttACGTTGGGCTGTTAACGCGTACCCCGCGCTATGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGAcg                                                               <  2:516491/90‑1 (MQ=255)

                       ttACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGAcg                                                               >  1:2931162/1‑90 (MQ=255)

                       ttACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGAcg                                                               <  2:246189/90‑1 (MQ=255)

                       ttACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGAcg                                                               >  1:1521525/1‑90 (MQ=255)

                        tACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGAcgc                                                              <  2:1503532/90‑1 (MQ=255)

                        tACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGAcgc                                                              >  1:1995824/1‑90 (MQ=255)

                        tACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGAcgc                                                              <  1:737777/90‑1 (MQ=255)

                          cGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCgg                                                            <  2:569112/90‑1 (MQ=255)

                           gTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCggg                                                           >  1:777509/1‑90 (MQ=255)

                           gTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCggg                                                           >  1:1003913/1‑90 (MQ=255)

                           gTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCggg                                                           >  2:1696437/1‑90 (MQ=255)

                             tGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCt                                                         >  1:1052808/1‑90 (MQ=255)

                               ggCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTAt                                                       >  2:522849/1‑90 (MQ=255)

                                gCTGTTAACGCGTACCCCGCGCTACGCCGCCGTGACGACCNCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATg                                                      <  2:1818826/90‑1 (MQ=255)

                                gCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATg                                                      >  2:2659622/1‑90 (MQ=255)

                                 cTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGc                                                     <  2:319373/90‑1 (MQ=255)

                                 cTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGc                                                     >  1:2254211/1‑90 (MQ=255)

                                     tAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGGACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCAcc                                                 <  2:820384/90‑1 (MQ=255)

                                     tAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCAcc                                                 >  1:2444978/1‑90 (MQ=255)

                                     tAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCAcc                                                 <  2:3018536/90‑1 (MQ=255)

                                      aaCGCGTACCCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCg                                                <  2:491291/90‑1 (MQ=255)

                                      aaCGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCg                                                <  1:2303409/90‑1 (MQ=255)

                                       aCGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGa                                               <  2:640772/90‑1 (MQ=255)

                                       aCGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGa                                               <  2:2317274/90‑1 (MQ=255)

                                       aCGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGa                                               <  2:2077352/90‑1 (MQ=255)

                                          cgTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATcc                                            >  1:1044267/1‑90 (MQ=255)

                                            tAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTc                                          >  1:689455/1‑90 (MQ=255)

                                            tAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTc                                          >  1:3457536/1‑90 (MQ=255)

                                             aaCCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCa                                         >  2:2941866/1‑90 (MQ=255)

                                              aCCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCAc                                        <  1:2934256/90‑1 (MQ=255)

                                               cccGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACt                                       <  1:356582/90‑1 (MQ=255)

                                                ccGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTa                                      >  2:1338844/1‑90 (MQ=255)

                                                 cgcgCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTAt                                     >  2:1497738/1‑90 (MQ=255)

                                                  gcgcTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATg                                    <  2:2229111/90‑1 (MQ=255)

                                                    gcTCCGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGcc                                  <  1:501054/90‑1 (MQ=255)

                                                    gcTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGcc                                  >  2:2898892/1‑90 (MQ=255)

                                                     cTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGccc                                 <  2:1964040/90‑1 (MQ=255)

                                                     cTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGccc                                 <  1:215788/90‑1 (MQ=255)

                                                       aCGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGc                               <  1:602367/90‑1 (MQ=255)

                                                        cgccgcCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCa                              <  2:32073/90‑1 (MQ=255)

                                                        cgccgcCGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCa                              <  1:1167240/90‑1 (MQ=255)

                                                         gccgccGTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCaa                             >  1:2560284/1‑90 (MQ=255)

                                                           cgccgTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAAc                           <  1:159000/90‑1 (MQ=255)

                                                            gccgTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACt                          <  2:340404/90‑1 (MQ=255)

                                                              cgTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCa                        >  1:477909/1‑90 (MQ=255)

                                                              cgTGACGACCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCa                        <  1:2094695/90‑1 (MQ=255)

                                                                    gacCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACa                  >  2:2911931/1‑90 (MQ=255)

                                                                    gacCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACa                  <  1:25498/90‑1 (MQ=255)

                                                                    gacCGCCGCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACa                  <  2:805569/90‑1 (MQ=255)

                                                                      ccgccgCGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGCTCCTCACTATGCCCGCAAACTCACCAACATg                <  2:2874618/90‑1 (MQ=255)

                                                                       cgccgcGAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGa               >  1:3366042/1‑90 (MQ=255)

                                                                          cgcgAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTc            <  1:1034959/90‑1 (MQ=255)

                                                                           gcgAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTca           <  2:2814296/90‑1 (MQ=255)

                                                                            cgAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGNCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTcag          <  2:3373989/90‑1 (MQ=255)

                                                                            cgAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTcag          >  1:663604/1‑90 (MQ=255)

                                                                            cgAGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTcag          >  2:1341179/1‑90 (MQ=255)

                                                                              aGTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTcagca        <  2:1598544/90‑1 (MQ=255)

                                                                               gTGCGGAACAGGGGGGCGCAGGCCCTTCAGGACGCGGGCTATGCCCCCGATCTTCACTATGCCCGCAAACTCACCAACATGATTcagcag       <  1:271501/90‑1 (MQ=255)

                                                                               gTGCGGAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTcagcag       <  1:1550475/90‑1 (MQ=255)

                                                                                   ggAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGa   >  1:277847/1‑90 (MQ=255)

                                                                                   ggAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGa   <  2:1408146/90‑1 (MQ=255)

                                                                                   ggAACAGGGGGGCACAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGa   >  2:1635069/1‑90 (MQ=255)

                                                                                    gAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGaa  >  2:390745/1‑90 (MQ=255)

                                                                                    gAACAGGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGaa  >  1:492321/1‑90 (MQ=255)

TCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACA‑GGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAA  >  AC_000091/1139659‑1139831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`