BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AC_000091	654,214	Δ1,199 bp		[dcuC]–[dcuC]	[dcuC], insH, [dcuC]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AC_000091	654214–655411	655412	2–1199	148 [0]	[0] 149	[dcuC]–[dcuC]	[dcuC],insH,[dcuC]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AC_000091	= 654213	0 (0.000)	136 (1.080)	92/168	0.1	100%	coding (70/477 nt)	dcuC	anaerobic C4‑dicarboxylate transport
*	?	AC_000091	655413 =	0 (0.000)	136 (1.080)	92/168	0.1	100%	coding (978/1092 nt)	dcuC	anaerobic C4‑dicarboxylate transport

Only 100 of 149 total aligned reads displayed.

GGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATAAGCCACTTCCAGACCAGA  >  AC_000091/655326‑655502

caccaataccagcatcaggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagccc                                                                                         <  1:1986950‑M2/3‑1 (MQ=255)

caccaataccagcatcaggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagccc                                                                                         >  1:1936842‑M2/88‑90 (MQ=255)

 accaataccagcatcaggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagcccc                                                                                        >  2:1046740‑M2/87‑90 (MQ=255)

  ccaataccagcatcaggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCt                                                                                       <  2:826721‑M2/5‑1 (MQ=255)

  ccaataccagcatcaggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCt                                                                                       <  1:106014‑M2/5‑1 (MQ=255)

  ccaataccagcatcaggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCt                                                                                       >  2:744303‑M2/86‑90 (MQ=255)

     ataccagcatcaggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAg                                                                                    >  1:2139072‑M2/83‑90 (MQ=255)

      taccagcatcaggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGc                                                                                   <  2:3256236‑M2/9‑1 (MQ=255)

      taccagcatcaggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGc                                                                                   <  1:2460619‑M2/9‑1 (MQ=255)

      taccagcatcaggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGc                                                                                   <  1:3454197‑M2/9‑1 (MQ=255)

      taccagcatcaggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGc                                                                                   <  1:1279084‑M2/9‑1 (MQ=255)

       accagcatcaggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCg                                                                                  >  2:704538‑M2/81‑90 (MQ=255)

       accagcatcaggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCg                                                                                  >  1:2969875‑M2/81‑90 (MQ=255)

       accagcatcaggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCg                                                                                  >  2:1833657‑M2/81‑90 (MQ=255)

         cagcatcaggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGaa                                                                                <  1:3092072‑M2/12‑1 (MQ=255)

         cagcatcaggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGaa                                                                                >  2:3445495‑M2/79‑90 (MQ=255)

           gcatcaggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATa                                                                              <  2:31214‑M2/14‑1 (MQ=255)

             atcaggatgatactcgccgaaccaaacgaggcagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATAcc                                                                            >  1:1656847‑M2/75‑90 (MQ=255)

              tcaggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATAccc                                                                           <  2:3342281‑M2/17‑1 (MQ=255)

              tcaggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATAccc                                                                           >  1:3374315‑M2/74‑90 (MQ=255)

                aggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCa                                                                         <  1:1544811‑M2/19‑1 (MQ=255)

                aggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCa                                                                         <  2:1676434‑M2/19‑1 (MQ=255)

                aggatgatactcgccgaaccaaacgaggtagcgatacaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCa                                                                         >  1:3299886‑M2/72‑90 (MQ=255)

                 ggatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAAaaaccccc                                                                        >  2:3424580‑M2/71‑88 (MQ=255)

                  gatgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGc                                                                       <  2:328779‑M2/21‑1 (MQ=255)

                   atgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCg                                                                      <  2:2755808‑M2/22‑1 (MQ=255)

                   atgatactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCg                                                                      <  1:265678‑M2/22‑1 (MQ=255)

                       tactcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCaa                                                                  >  2:3440959‑M2/65‑90 (MQ=255)

                         ctcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAAcc                                                                <  2:3106423‑M2/28‑1 (MQ=255)

                          tcgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACca                                                               <  2:3385637‑M2/29‑1 (MQ=255)

                           cgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACcag                                                              >  1:3083977‑M2/61‑90 (MQ=255)

                           cgccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACcag                                                              <  1:1583993‑M2/30‑1 (MQ=255)

                            gccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACcagc                                                             >  2:3219‑M2/60‑90 (MQ=255)

                             ccgaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACcagca                                                            >  2:2234793‑M2/59‑90 (MQ=255)

                               gaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCagcagc                                                          >  1:2691625‑M2/57‑90 (MQ=255)

                               gaaccaaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCagcagc                                                          >  2:1532949‑M2/57‑90 (MQ=255)

                                   caaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATca                                                      >  2:2757163‑M2/53‑90 (MQ=255)

                                    aaacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACAAGCAGCATcac                                                     >  2:2741616‑M2/52‑90 (MQ=255)

                                     aacgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATcacc                                                    >  2:2583365‑M2/51‑90 (MQ=255)

                                       cgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATcaccac                                                  <  1:1723857‑M2/42‑1 (MQ=255)

                                       cgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATcaccac                                                  >  1:370004‑M2/49‑90 (MQ=255)

                                       cgaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATcaccac                                                  <  1:589704‑M2/42‑1 (MQ=255)

                                        gaggtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACg                                                 <  2:1463751‑M2/43‑1 (MQ=255)

                                           gtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTa                                              >  2:655253‑M2/45‑90 (MQ=255)

                                           gtagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTa                                              >  1:211262‑M2/45‑90 (MQ=255)

                                            tagcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAg                                             <  1:262243‑M2/47‑1 (MQ=255)

                                             agcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGc                                            >  1:262664‑M2/43‑90 (MQ=255)

                                              gcgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCa                                           <  1:766361‑M2/49‑1 (MQ=255)

                                               cgatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCaa                                          <  2:437318‑M2/50‑1 (MQ=255)

                                                gatagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCaaa                                         >  1:110953‑M2/40‑90 (MQ=255)

                                                 atagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAAc                                        <  2:1478252‑M2/52‑1 (MQ=255)

                                                 atagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAAc                                        >  1:2376113‑M2/39‑90 (MQ=255)

                                                 atagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAAc                                        <  1:3307973‑M2/52‑1 (MQ=255)

                                                  tagaaatcagactttgaataaagctgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACg                                       <  1:1117669‑M2/53‑1 (MQ=255)

                                                  tagaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACg                                       >  2:2556888‑M2/38‑90 (MQ=255)

                                                   agaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGc                                      <  2:245869‑M2/54‑1 (MQ=255)

                                                    gaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATGCCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCa                                     <  1:1517943‑M2/55‑1 (MQ=255)

                                                    gaaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCa                                     <  2:1904650‑M2/55‑1 (MQ=255)

                                                     aaatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCAt                                    <  1:2871509‑M2/56‑1 (MQ=255)

                                                      aatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATc                                   <  2:2944674‑M2/57‑1 (MQ=255)

                                                      aatcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATc                                   <  1:1166716‑M2/57‑1 (MQ=255)

                                                       atcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCt                                  >  1:1641967‑M2/33‑90 (MQ=255)

                                                       atcagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCt                                  >  1:753185‑M2/33‑90 (MQ=255)

                                                         cagactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGc                                <  1:3416919‑M2/60‑1 (MQ=255)

                                                          agactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGTAAACGCATCTGcc                               >  1:1238191‑M2/30‑90 (MQ=255)

                                                          agactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGcc                               <  2:609722‑M2/61‑1 (MQ=255)

                                                           gactttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCa                              <  2:3029334‑M2/62‑1 (MQ=255)

                                                            actttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCAt                             >  2:2357102‑M2/28‑90 (MQ=255)

                                                              tttgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATcc                           <  2:1834731‑M2/65‑1 (MQ=255)

                                                                tgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCcg                         >  1:1151381‑M2/24‑90 (MQ=255)

                                                                tgaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCcg                         >  1:647694‑M2/24‑90 (MQ=255)

                                                                 gaataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCcgc                        >  1:2050855‑M2/23‑90 (MQ=255)

                                                                  aataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCcgcg                       >  1:906957‑M2/22‑90 (MQ=255)

                                                                   ataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGa                      <  1:1110507‑M2/70‑1 (MQ=255)

                                                                   ataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGa                      <  1:2398147‑M2/70‑1 (MQ=255)

                                                                   ataaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGa                      <  2:1759337‑M2/70‑1 (MQ=255)

                                                                    taaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGAt                     >  2:2125504‑M2/20‑90 (MQ=255)

                                                                    taaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGAt                     >  2:359048‑M2/20‑90 (MQ=255)

                                                                    taaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGAt                     <  2:1921344‑M2/71‑1 (MQ=255)

                                                                    taaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGAt                     <  2:1637703‑M2/71‑1 (MQ=255)

                                                                     aaagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATa                    >  1:3247781‑M2/19‑90 (MQ=255)

                                                                      aagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATaa                   >  1:807412‑M2/18‑90 (MQ=255)

                                                                      aagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATaa                   >  2:286347‑M2/18‑90 (MQ=255)

                                                                      aagccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATaa                   >  1:2472567‑M2/18‑90 (MQ=255)

                                                                       agccgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATAAg                  <  1:2212834‑M2/74‑1 (MQ=255)

                                                                          cgatggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATAAGCCa               >  1:133797‑M2/14‑90 (MQ=255)

                                                                            atggtgctaagTCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATAAGCCACt             <  1:2988337‑M2/79‑1 (MQ=255)

                                                                            atggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATAAGCCACt             >  2:1060067‑M2/12‑90 (MQ=255)

                                                                            atggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATAAGCCACt             >  1:2850225‑M2/12‑90 (MQ=255)

                                                                             tggtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATAAGCCACtt            >  2:2713416‑M2/11‑90 (MQ=255)

                                                                               gtgctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATAAGCCACTTcc          >  2:316752‑M2/9‑90 (MQ=255)

                                                                                 tctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATAAGCCACTTccag        <  2:301997‑M2/84‑1 (MQ=255)

                                                                                 gctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATAAGCCACTTccag        >  2:2615249‑M2/7‑90 (MQ=255)

                                                                                 gctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATAAGCCACTTccag        <  2:893742‑M2/84‑1 (MQ=255)

                                                                                  ctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATAAGCCACTTccaga       >  2:157887‑M2/6‑90 (MQ=255)

                                                                                   taagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATAAGCCACTTccagac      <  2:2625282‑M2/86‑1 (MQ=255)

                                                                                   taagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATAAGCCACTTccagac      <  2:1545005‑M2/86‑1 (MQ=255)

                                                                                     agCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATAAGCCACTTccagacca    <  2:1168507‑M2/88‑1 (MQ=255)

                                                                                      gCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATAAGCCACTTccagaccag   >  1:320832‑M2/2‑90 (MQ=255)

                                                                                       ccccTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATAAGCCACTTccagaccaga  <  2:4512/90‑1 (MQ=255)

GGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCGATAAGCCACTTCCAGACCAGA  >  AC_000091/655326‑655502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`