Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AC_000091119,3840TG27.8% 277.5 / 18.6 115V35G (GTG→GGG) ampEpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (7/76);  new base G (32/0);  total (39/76)
Fisher's exact test for biased strand distribution p-value = 5.46e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 133 total aligned reads displayed.

AACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTAT  >  AC_000091/119295‑119473
                                                                                         |                                                                                         
aaCCTTACTGGTGTTAATTTTCGAGCGTCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGt                                                                                           <  2:2853977/90‑1 (MQ=255)
aaCCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGt                                                                                           <  2:2411555/90‑1 (MQ=255)
   cTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGaa                                                                                        >  1:1955251/1‑90 (MQ=255)
    ttACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGaaa                                                                                       >  1:822886/1‑90 (MQ=255)
     tACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAAc                                                                                      <  1:28284/90‑1 (MQ=255)
     tACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAAc                                                                                      <  2:2174712/90‑1 (MQ=255)
       cTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACAt                                                                                    <  1:99455/90‑1 (MQ=255)
        tGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAAAAtt                                                                                   >  2:2529208/1‑90 (MQ=255)
         ggTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACAttt                                                                                  <  1:2204030/90‑1 (MQ=255)
         ggTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAAAAttt                                                                                  >  1:1931962/1‑90 (MQ=255)
         ggTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGAACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAAAAttt                                                                                  >  2:304538/1‑90 (MQ=255)
           tgtTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACAttttt                                                                                <  1:3234750/90‑1 (MQ=255)
           tgtTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAAAAttttt                                                                                >  1:2279578/1‑90 (MQ=255)
               aaTTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTtctct                                                                            <  1:536906/90‑1 (MQ=255)
               aaTTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTtctct                                                                            <  2:102922/90‑1 (MQ=255)
                aTTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTtttctc                                                                           >  1:2175080/1‑90 (MQ=255)
                 ttttCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTCTCTCg                                                                          >  2:1338637/1‑90 (MQ=255)
                   ttCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCggg                                                                        <  1:458210/90‑1 (MQ=255)
                    tCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGgc                                                                       <  1:1991104/90‑1 (MQ=255)
                     cGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTCTCGGgcg                                                                      >  1:946798/1‑90 (MQ=255)
                      gAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGgcgc                                                                     <  2:3283934/90‑1 (MQ=255)
                      gAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAAAATTTTTTTCTCGGgcgc                                                                     >  1:710293/1‑90 (MQ=255)
                      gAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAAAATTTTTTTTTCGGgggc                                                                     >  2:2971501/1‑90 (MQ=255)
                       aGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCa                                                                    <  2:18261/90‑1 (MQ=255)
                        gcgcCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCAc                                                                   <  2:138698/90‑1 (MQ=255)
                        gcgcCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCAc                                                                   <  2:1625477/90‑1 (MQ=255)
                        gcgcCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAACATTTTTTTCTCCGGCGCAc                                                                   >  1:2867948/1‑90 (MQ=255)
                          gcCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAAAATTTTTCTCTCGGGCGCACGt                                                                 >  1:3442559/1‑90 (MQ=255)
                           ccTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTTTCTCGGGGGCCCGtt                                                                >  1:3272139/1‑90 (MQ=255)
                           ccTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGtt                                                                <  2:62918/90‑1 (MQ=255)
                           ccTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGtt                                                                <  1:1018812/90‑1 (MQ=255)
                           ccTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGtt                                                                <  1:1922591/90‑1 (MQ=255)
                            cTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTa                                                               <  2:518033/90‑1 (MQ=255)
                            cTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTa                                                               <  2:3151397/90‑1 (MQ=255)
                             tGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAg                                                              <  2:1876953/90‑1 (MQ=255)
                              gTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAAAATTTTTCTCTCGGGCGCACGTTAgg                                                             >  1:2614792/1‑90 (MQ=255)
                               tttAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTCTCGGGCGCACGTTAGGc                                                            >  1:836237/1‑90 (MQ=255)
                               tttAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGCGAAAAATTTTTTTCTCGGGGGCCCGGTAGGc                                                            >  1:686404/1‑90 (MQ=255)
                               tttAAGTTGGGCGAGCACTGGCAGCTTAATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAAAATTTTTCTCTCGGGCGGCCGTTAggg                                                            >  2:3448639/1‑89 (MQ=255)
                                  aaGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACCATTTTTTCTCCGGCGCACGTTAGGGATg                                                         >  2:2976950/1‑90 (MQ=255)
                                   aGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTCTCTCGGGCGCCCGTTAGGCATGa                                                        >  2:81430/1‑90 (MQ=255)
                                    gTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAAAATTTTTCTTTCGGGGGCCCGGTAGGCCTGAc                                                       >  1:1853565/1‑90 (MQ=255)
                                     ttGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGAcc                                                      <  1:1008858/90‑1 (MQ=255)
                                     ttGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGAcc                                                      <  2:824467/90‑1 (MQ=255)
                                     ttGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAAAATTTTTCTCTCGGGCGCACGGTAGGCATGAcc                                                      >  2:1911847/1‑90 (MQ=255)
                                        ggCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCatt                                                   <  1:2455257/90‑1 (MQ=255)
                                          cGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCattat                                                 <  1:1797751/90‑1 (MQ=255)
                                          cGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAACATTTTTCTCTCGGGCGCCCGTTAGGCATGACCattat                                                 >  1:180205/1‑90 (MQ=255)
                                          cGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTCTCTCGGGGGCACGGTAGGCATGACCattat                                                 >  1:1670553/1‑90 (MQ=255)
                                            aGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTCTTCTCTCGGGCGCACGTTAGGCATGACCATTATTg                                               <  2:2529535/90‑1 (MQ=255)
                                              cACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCg                                             <  1:448703/90‑1 (MQ=255)
                                              cACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTCTCTCGGGCGCCCGTTAGGCATGACCATTATTGCg                                             >  1:1371211/1‑90 (MQ=255)
                                                cTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGAt                                           <  1:275666/90‑1 (MQ=255)
                                                cTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGAt                                           <  1:1947659/90‑1 (MQ=255)
                                                cTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTCTCGGGCGCACGTTTGGCATGACCCTTATTGCggt                                           >  1:2316011/1‑88 (MQ=255)
                                                 tGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAACATTTTTCTCTCTGGCGCCCGTTAGGCATGACCATTATTGCGATg                                          >  1:3254822/1‑90 (MQ=255)
                                                   gCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATggg                                        <  2:710990/90‑1 (MQ=255)
                                                   gCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATggg                                        <  2:3087854/90‑1 (MQ=255)
                                                   gCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAAAATTTTTCTCTCGGGCGCACGGTAGGCATGACCATTATTGCGATggg                                        >  1:835653/1‑90 (MQ=255)
                                                     aGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCg                                      <  1:2311469/90‑1 (MQ=255)
                                                     aGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAAAATTTTTCTCTCGGGCGCACGGTAGGCATGACCCTTATTTCGATGGGCg                                      >  2:2981495/1‑90 (MQ=255)
                                                        ttGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGa                                   <  2:615856/90‑1 (MQ=255)
                                                               tcGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACtttttt                            <  2:456892/90‑1 (MQ=255)
                                                                 gTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTAc                          <  1:2561627/90‑1 (MQ=255)
                                                                 gTCTTGAAGCGTTCTTTCGGCGGGGGGAACATTTTTTTCTCGGGGGCGCGTTAGGCATGACCATTATTGCGATGGGGGTGACTTTTTTAc                          >  1:770579/1‑90 (MQ=255)
                                                                 gTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGGGTGACgttttttc                          >  1:2506414/1‑88 (MQ=255)
                                                                 gTCTTGAAGCGTTCTTTCGGCGGGGGAAAAATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACtttttttc                          >  1:1772787/1‑88 (MQ=255)
                                                                  tCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACt                         <  1:2909553/90‑1 (MQ=255)
                                                                   cTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTCTCTCGGGGGCACGTTAGGCATGACCATTATTGCGATGGGCGGGACTTTTTTTCTg                        >  2:3239352/1‑90 (MQ=255)
                                                                    ttGAAGCGTTCTTTCGGCGGGTGGAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGGTGGGCGTGACTTTTTTTCTGt                       >  1:2174315/1‑90 (MQ=255)
                                                                    ttGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGt                       <  2:2768195/90‑1 (MQ=255)
                                                                     tGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGCCCATTATTGCGATGGGCGTGACTTTTTTACTGtt                      <  2:352791/90‑1 (MQ=255)
                                                                     tGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGtt                      <  1:1760404/90‑1 (MQ=255)
                                                                       aaGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTAc                    <  2:2211413/90‑1 (MQ=255)
                                                                        aGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGTACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTAcg                   <  1:539189/90‑1 (MQ=255)
                                                                          cGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTAcgcg                 <  2:1322518/90‑1 (MQ=255)
                                                                          cGTTCTTTCGGCGGGGGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTAcgcg                 >  1:2025834/1‑90 (MQ=255)
                                                                           gTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACgcgc                <  2:3432441/90‑1 (MQ=255)
                                                                             tCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCAt              <  1:3428256/90‑1 (MQ=255)
                                                                              cTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATTGGCGTGACTTTTTTACTGTTACGCGCAtt             <  2:1676260/90‑1 (MQ=255)
                                                                              cTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCAtt             <  2:2260909/90‑1 (MQ=255)
                                                                              cTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCAtt             <  1:422561/90‑1 (MQ=255)
                                                                              cTTTCGGCGGGGGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCAtt             >  1:601489/1‑90 (MQ=255)
                                                                               tttCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTg            <  1:2707610/90‑1 (MQ=255)
                                                                               tttCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTg            <  2:3353960/90‑1 (MQ=255)
                                                                                ttCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGc           <  1:1501360/90‑1 (MQ=255)
                                                                                ttCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGc           <  1:2730058/90‑1 (MQ=255)
                                                                                ttCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGc           <  1:1184844/90‑1 (MQ=255)
                                                                                 tCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCa          <  1:1015457/90‑1 (MQ=255)
                                                                                 tCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCa          >  1:470185/1‑90 (MQ=255)
                                                                                 tCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCa          <  1:2752337/90‑1 (MQ=255)
                                                                                    gcggGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAggg       <  2:3392071/90‑1 (MQ=255)
                                                                                     cggGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGa      <  2:3069410/90‑1 (MQ=255)
                                                                                     cggGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGa      >  2:2826593/1‑90 (MQ=255)
                                                                                     cggGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGa      <  2:879949/90‑1 (MQ=255)
                                                                                       ggTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGt    <  2:3367934/90‑1 (MQ=255)
                                                                                       ggTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGt    <  1:2694919/90‑1 (MQ=255)
                                                                                        gTGAAACATTTTTCTCTTGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTa   >  2:3447648/1‑90 (MQ=255)
                                                                                        gTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTa   <  2:1905415/90‑1 (MQ=255)
                                                                                         tGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTAt  >  1:1552976/1‑90 (MQ=255)
                                                                                         |                                                                                         
AACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTAT  >  AC_000091/119295‑119473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑