Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AC_0000911,163,0180TG23.6% 226.3 / 16.8 106A37A (GCA→GCC) fhuEferric‑rhodotorulic acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base T (24/57);  new base G (25/0);  total (49/57)
Fisher's exact test for biased strand distribution p-value = 4.96e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 121 total aligned reads displayed.

GCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCT  >  AC_000091/1162929‑1163107
                                                                                         |                                                                                         
gCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGt                                                                                           <  1:2878123/90‑1 (MQ=255)
  agagGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGgc                                                                                         >  2:2736984/1‑90 (MQ=255)
   gagGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTgca                                                                                        >  2:575652/1‑90 (MQ=255)
   gagGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGgca                                                                                        >  1:3369063/1‑90 (MQ=255)
    agGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTgcag                                                                                       <  2:1964250/90‑1 (MQ=255)
    agGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTgcag                                                                                       <  1:3155574/90‑1 (MQ=255)
      gTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGgcagca                                                                                     >  1:1852837/1‑90 (MQ=255)
        aGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCaaa                                                                                   >  2:1990308/1‑90 (MQ=255)
        aGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCaaa                                                                                   >  2:370953/1‑90 (MQ=255)
         gACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCaaaa                                                                                  <  1:2597375/90‑1 (MQ=255)
           cGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGGAGCcaaaaa                                                                                >  1:2368213/1‑88 (MQ=255)
           cGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGc                                                                                >  2:412020/1‑90 (MQ=255)
                cGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAg                                                                           <  2:2812966/90‑1 (MQ=255)
                cGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGGAGCAAAAGCGGCAg                                                                           >  1:3478610/1‑90 (MQ=255)
                 gCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGa                                                                          <  2:726631/90‑1 (MQ=255)
                   tGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAg                                                                        <  1:2904057/90‑1 (MQ=255)
                   tGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAACGGCAGAAg                                                                        >  1:688462/1‑90 (MQ=255)
                    gTAATCATTTTCGCCATCATCTGGAGCTGTGTATGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAgg                                                                       <  2:1578754/90‑1 (MQ=255)
                       aTCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCATTGGCTGGTGCAGCAAAAGCGGCAGAAGGTaa                                                                    <  2:1311264/90‑1 (MQ=255)
                       aTCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTaa                                                                    <  2:3154765/90‑1 (MQ=255)
                       aTCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTaa                                                                    <  2:107127/90‑1 (MQ=255)
                        tCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAACGGCAGAAGGGaaa                                                                   >  2:3256415/1‑89 (MQ=255)
                          aTTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGGAATAg                                                                 >  1:3095880/1‑90 (MQ=255)
                          aTTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCGGGAAAAGCGGCCGGAGGTAATAg                                                                 >  1:1069/1‑90 (MQ=255)
                           ttttCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGt                                                                <  2:196163/90‑1 (MQ=255)
                           ttttCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGt                                                                <  2:1101661/90‑1 (MQ=255)
                            tttCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCACAAGCGGCAGAAGGTAATAGTg                                                               <  2:3104621/90‑1 (MQ=255)
                            tttCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAATGTAATAGTg                                                               >  1:2262569/1‑90 (MQ=255)
                            tttCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTg                                                               <  2:2942048/90‑1 (MQ=255)
                             ttCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTCCAGCAAAAGCGGCAGAAGCTAATAGTgt                                                              <  2:1396427/90‑2 (MQ=255)
                                gCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAg                                                           <  2:876665/90‑1 (MQ=255)
                                 ccaccaTCTGGAGCTGTGGCTGAACCCTCAACAATCCCCGTTTCTTCAGGGGCTGGTGCAGCAAAAGCGGCAGAAGTTAATAGTGCCAGt                                                          <  2:2600016/90‑1 (MQ=255)
                                 ccATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGt                                                          <  2:1182334/90‑1 (MQ=255)
                                 ccATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGt                                                          >  1:309383/1‑90 (MQ=255)
                                 ccATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCCGGAAAAGCGGCAGAAGGTAAAAGTGCCAga                                                          >  2:2286808/1‑89 (MQ=255)
                                  catcatCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTg                                                         <  2:1833077/90‑1 (MQ=255)
                                  catcatCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGGAATAGTGCCAGTg                                                         >  1:270173/1‑90 (MQ=255)
                                  catcatCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGAGGCAGAAGGTAATAGGGCCAGTg                                                         >  2:2156293/1‑90 (MQ=255)
                                    tcatcTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCt                                                       <  2:2235256/90‑1 (MQ=255)
                                     catcTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTa                                                      <  2:2608680/90‑1 (MQ=255)
                                      atcTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCGGCAAAAGCGGCGGAAGGGAATAGTGCCAGTGCTAt                                                     >  1:993194/1‑90 (MQ=255)
                                      atcTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTAt                                                     <  2:1807207/90‑1 (MQ=255)
                                      atcTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGGAATAGTGCCCGTGCTAt                                                     >  1:2531474/1‑90 (MQ=255)
                                       tcTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCTGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATg                                                    >  2:1802401/1‑90 (MQ=255)
                                       tcTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGAGCCAGTGCTATg                                                    >  1:3330063/1‑90 (MQ=255)
                                        cTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCCAAAGCGGCAGAAAGGAATAGTGCCAGTGCTATGc                                                   >  1:2782855/1‑90 (MQ=255)
                                         tGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCGGCAGAAGGGAATAGGGCCAGTGCTATGCa                                                  >  2:686862/1‑90 (MQ=255)
                                         tGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAACGGCAGAAGGGAATAGTGCCAGTGCTATGCa                                                  >  2:522338/1‑90 (MQ=255)
                                           gAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCGGCAGAAAGGGATAATGCCGGTGCTATGGaaa                                                >  2:772340/1‑89 (MQ=255)
                                            aGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAAcc                                               >  1:3260000/1‑90 (MQ=255)
                                             gCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCg                                              <  1:1634391/90‑1 (MQ=255)
                                             gCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCg                                              <  1:3087914/90‑1 (MQ=255)
                                              cTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCgg                                             >  1:808148/1‑90 (MQ=255)
                                               tgtgGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGc                                            <  1:1153814/90‑1 (MQ=255)
                                                 tgGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCaa                                          <  2:2906664/90‑1 (MQ=255)
                                                 tgGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCaa                                          <  1:1672355/90‑1 (MQ=255)
                                                  ggCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCGGCAGAAGGGAATAGGGCCAGTGCTATGCAACCGGCAag                                         >  1:2433443/1‑90 (MQ=255)
                                                   gCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAagt                                        <  2:1265479/90‑1 (MQ=255)
                                                   gCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCGGCAGAAGGGAATAGTGCCAGTGCTATGCAACCGGCAagt                                        >  2:1313315/1‑90 (MQ=255)
                                                     tGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCGGCAGAAGGAAATAGTGCCAGTGCTATGCAACCCGCAagtag                                      >  2:3060627/1‑90 (MQ=255)
                                                      gAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGGAATAGTGCCAGTGCTATGCAACCGGCAagtagt                                     >  1:2007814/1‑90 (MQ=255)
                                                      gAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAagtagt                                     >  2:2124208/1‑90 (MQ=255)
                                                       aaCCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTg                                    <  2:1537720/90‑1 (MQ=255)
                                                       aaCCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCGGCAGAAGGTAATAGGGCCAGTGCTATGCAACCGGCAAGTAGTg                                    >  1:1758325/1‑90 (MQ=255)
                                                        aCCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGa                                   <  1:2616210/90‑1 (MQ=255)
                                                         cccTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCGGCAGAAGGGAATAGTGCCAGTGCTATGCAACCGGCAAGGAGTGAc                                  >  2:2300391/1‑90 (MQ=255)
                                                          ccTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACg                                 <  1:3482900/90‑1 (MQ=255)
                                                                caaTCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTgg                           >  1:605437/1‑90 (MQ=255)
                                                                 aaTCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGt                          >  1:2108091/1‑90 (MQ=255)
                                                                 aaTCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGt                          >  1:1889945/1‑90 (MQ=255)
                                                                    cACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCGGCAGAAGGGAATAGTGCCAGTGCTATGCAACCGGCAAGGAGTGACGGGTTGGTGAt                       >  2:1839496/1‑90 (MQ=255)
                                                                     aCCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATg                      <  2:1502516/90‑1 (MQ=255)
                                                                     aCCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATg                      <  1:1141939/90‑1 (MQ=255)
                                                                        gTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGGCGGTTTGGTGATGGCt                   >  1:612609/1‑90 (MQ=255)
                                                                         tttCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCtt                  <  2:2548343/90‑1 (MQ=255)
                                                                          ttcttcAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTg                 >  1:1912019/1‑90 (MQ=255)
                                                                            cttcAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGat               <  1:1412183/90‑1 (MQ=255)
                                                                            cttcAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGat               <  2:565258/90‑1 (MQ=255)
                                                                             ttcAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGata              >  1:2845909/1‑90 (MQ=255)
                                                                             ttcAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGGAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGata              >  1:2081373/1‑90 (MQ=255)
                                                                               cAGTGGCTGGGGCAGCAAAAGCGGCAGAAGGGAATAGTGCCCGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATAtt            >  2:3334681/1‑90 (MQ=255)
                                                                                aGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTg           <  1:1802050/90‑1 (MQ=255)
                                                                                aGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTg           <  2:612853/90‑1 (MQ=255)
                                                                                 gTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGa          <  1:2037015/90‑1 (MQ=255)
                                                                                 gTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGa          >  1:2476407/1‑90 (MQ=255)
                                                                                 gTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGa          <  2:1835910/90‑1 (MQ=255)
                                                                                 gTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGGTTTGGGGTGGCTTTGTAttta          >  2:3210747/1‑88 (MQ=255)
                                                                                  tGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGAt         >  1:1405444/1‑90 (MQ=255)
                                                                                  tGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGAt         <  2:1275197/90‑1 (MQ=255)
                                                                                   ggCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGAtt        >  1:2093458/1‑90 (MQ=255)
                                                                                   ggCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGAtt        <  1:1150709/90‑1 (MQ=255)
                                                                                   ggCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGAtt        <  2:1086013/90‑1 (MQ=255)
                                                                                    gCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTa       >  2:3377605/1‑90 (MQ=255)
                                                                                      tGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATc     <  1:109423/90‑1 (MQ=255)
                                                                                        gTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATccc   <  2:1071702/90‑1 (MQ=255)
                                                                                         tGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCt  <  2:1968186/90‑1 (MQ=255)
                                                                                         tGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCt  >  1:628864/1‑90 (MQ=255)
                                                                                         tGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCt  >  1:2273434/1‑90 (MQ=255)
                                                                                         tGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCt  <  1:2207659/90‑1 (MQ=255)
                                                                                         tGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCt  >  1:1453430/1‑90 (MQ=255)
                                                                                         |                                                                                         
GCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCT  >  AC_000091/1162929‑1163107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑